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Addressing the needs of chikungunya fever outbreak control, here, we developed a flexible, ultrasensitive dual-mode lateral flow immunoassay (LFA) capable of simultaneous detection of chikungunya virus (CHIKV) antigen and the inflammatory cytokine IL-6 across clinical samples. An enhanced colorimetric-fluorescent dual-signal magnetic probe (MAu2QD3) was synthesized via electrostatic self-assembly. By tuning the number of loaded signal layers (Au and CdSe\/ZnS particles), the LFA platform achieved controllable adjustment of dual-signal output intensities. In parallel, an artificial intelligence-based dual-mode quantitative model was built to automatically integrate colorimetric and fluorescent readouts, yielding accurate target concentration estimates. In naked-eye mode, the assay exhibited visual limits of detection (LODs) of 50 pg\u00b7mL\u20131 for both CHIKV and IL-6. With portable instrument assistance, the colorimetric sensitivity of MAu2QD3-LFA increased by at least 4-fold, while fluorescent detection further reduced the LODs to 0.28 pg\u00b7mL\u20131 for CHIKV and 0.16 pg\u00b7mL\u20131 for IL-6. Validated using 91 clinical samples, the system achieved a sensitivity of 98.2% and a specificity of 100%, enabling reliable acquisition of both pathogen and host immune information. These results highlight strong potential for early surveillance and precise diagnosis of infectious diseases.<\/jats:p>","DOI":"10.1021\/acsami.6c10007","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T08:01:53Z","timestamp":1785139313000},"page":"40426-40442","source":"Crossref","is-referenced-by-count":0,"title":["Dual-Mode Field-Deployable Platform Based on Self-Assembled Magnetic Probes for Simultaneous Monitoring of Chikungunya Virus and Host Immune Status"],"prefix":"10.1021","volume":"18","author":[{"given":"Junyan","family":"Ai","sequence":"first","affiliation":[{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510515"]},{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxuan","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]},{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510515"]},{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meirou","family":"Lu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]},{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510515"]},{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9627-107X","authenticated-orcid":true,"given":"Chongwen","family":"Wang","sequence":"additional","affiliation":[{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510515"]},{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7764-3213","authenticated-orcid":true,"given":"Bing","family":"Gu","sequence":"additional","affiliation":[{"name":"Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510515"]},{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]},{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"issue":"1","key":"2026080518073724600_cit1","doi-asserted-by":"publisher","first-page":"3418","DOI":"10.1038\/s41467-025-58609-5","article-title":"The overlapping global distribution of dengue, chikungunya, Zika and yellow fever","volume":"16","author":"Lim","year":"2025","journal-title":"Nat. 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