{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:50:29Z","timestamp":1791557429198,"version":"4.3.4"},"reference-count":44,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"name":"Beijing Municipal Natural Science Foundation","award":["23JCZXJC00370"],"award-info":[{"award-number":["23JCZXJC00370"]}]},{"name":"Beijing Municipal Natural Science Foundation","award":["J230002"],"award-info":[{"award-number":["J230002"]}]},{"name":"Natural Science Foundation of Tianjin City","award":["22JCZDJC00230"],"award-info":[{"award-number":["22JCZDJC00230"]}]},{"name":"Tianjin Natural Science Foundation for Distinguished Young Scholars","award":["23JCJQJC00210"],"award-info":[{"award-number":["23JCJQJC00210"]}]},{"name":"Shanghai \"Pioneer Program\" Bio-Hybrid Robot Project","award":["25XF3200800"],"award-info":[{"award-number":["25XF3200800"]}]},{"name":"National Basic Research Program of China (973 Program)","award":["2021YFA0909300"],"award-info":[{"award-number":["2021YFA0909300"]}]},{"name":"National Natural Science Foundation of China (NSFC)","award":["32122047"],"award-info":[{"award-number":["32122047"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Natural Science Foundation of China (NSFC)","award":["32571637"],"award-info":[{"award-number":["32571637"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Project of National Engineering Research Center for Petroleum Refining Technology and Catalyst"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Engineered yeast-living drugs offer significant potential for oral protein therapy but remain limited by poor intestinal retention and lack of precise spatiotemporal control over therapeutic delivery. Here, we developed an ingestible hydrogel-encapsulated optogenetic yeast system controlled by a wearable electronic device for programmable interleukin-10 (IL-10) delivery in inflammatory bowel disease (IBD). We engineered Pichia pastoris with an EL222-based blue-light-inducible circuit achieving rapid, stringent IL-10 secretion (50% saturated concentration within just 1 h upon induction). The yeast was encapsulated in a photo-crosslinkable HAMA hydrogel that was gelated within 60 s upon blue-light exposure, exhibiting robust mechanical stability and tissue adhesion. A lightweight (2.27 g) wearable Bluetooth-controlled electronic device delivered targeted blue light transdermally, enabling simultaneous in situ hydrogel crosslinking and optogenetic activation in yeast cells. This system significantly extended the time yeast stays in the mouse gut, with reporter gene expression lasting over 5 h, while the unencapsulated control group lasted less than 2 h. In DSS-induced colitis mice, our strategy reduced proinflammatory cytokines (IL-1\u03b2, IL-6, and TNF-\u03b1) by &amp;gt;60%, restored intestinal barrier integrity, and normalized gut microbiota diversity to near-healthy levels, which performed significantly better than in other groups. This integrated \u201cextracorporeal instruction\u2212intracorporeal response\u201d paradigm establishes a digitally programmable platform for precision live biotherapeutics. However, limited blue-light tissue penetration remains a key constraint; future work should focus on near-infrared photosensitive systems, programmable-degradation hydrogels, and wireless closed\u2011loop feedback to advance clinical translation.<\/jats:p>","DOI":"10.1021\/acsami.6c10319","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T17:03:11Z","timestamp":1786726991000},"page":"45037-45053","source":"Crossref","is-referenced-by-count":1,"title":["Ingestible Hydrogel-Encapsulated Optogenetic Yeast Controlled by Wearable Electronics Enables Programmable IL-10 Delivery for Inflammatory Bowel Disease Therapy"],"prefix":"10.1021","volume":"18","author":[{"given":"Chao","family":"Zhang","sequence":"first","affiliation":[{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]},{"name":"Hainan University , , ,","place":["Haikou, China, 570228"]},{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxiang","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]},{"name":"Hainan University , , ,","place":["Haikou, China, 570228"]},{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Jiang","sequence":"additional","affiliation":[{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Geriatrics Institute , , ,","place":["Tianjin, China, 300052"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijie","family":"Feng","sequence":"additional","affiliation":[{"name":"Hainan University , , ,","place":["Haikou, China, 570228"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Shi","sequence":"additional","affiliation":[{"name":"Peking University Third Hospital , , ,","place":["Beijing, China, 100191"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0867-1370","authenticated-orcid":true,"given":"Duo","family":"Liu","sequence":"additional","affiliation":[{"name":"Hainan University , , ,","place":["Haikou, China, 570228"]},{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]},{"name":"Hainan University , , ,","place":["Haikou, China, 570228"]},{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]},{"name":"Longyan University , , ,","place":["Fujian, China, 364012"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"2026091814061815000_cit1","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1002\/yea.70009","article-title":"The power of yeast","volume":"42","author":"Nielsen","year":"2025","journal-title":"Yeast"},{"key":"2026091814061815000_cit2","doi-asserted-by":"publisher","first-page":"5867","DOI":"10.1002\/jcp.29583","article-title":"Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins","volume":"235","author":"Karbalaei","year":"2020","journal-title":"J. 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