{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T08:52:40Z","timestamp":1791967960261,"version":"4.3.4"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"40","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100010870","name":"Qingdao Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["26-1-5-cspz-23-nsh"],"award-info":[{"award-number":["26-1-5-cspz-23-nsh"]}],"id":[{"id":"10.13039\/501100010870","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFE0104100"],"award-info":[{"award-number":["2024YFE0104100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011324","name":"Qingdao University","doi-asserted-by":"publisher","award":["YX2024201"],"award-info":[{"award-number":["YX2024201"]}],"id":[{"id":"10.13039\/501100011324","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22274083"],"award-info":[{"award-number":["22274083"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22574089"],"award-info":[{"award-number":["22574089"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2022LZY022"],"award-info":[{"award-number":["ZR2022LZY022"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2023LZY005"],"award-info":[{"award-number":["ZR2023LZY005"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Qingdao City","award":["2022-4-005-YY"],"award-info":[{"award-number":["2022-4-005-YY"]}]},{"name":"State Key Laboratory of BioFibers and EcoTextiles, Qingdao University","award":["TSKT202101"],"award-info":[{"award-number":["TSKT202101"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Wound infections caused by fungi and bacteria remain a major global health challenge. In this study, 2,6-dimethoxy-1,4-benzoquinone (DMBQ) was isolated from Ilicis Rotundae Cortex (IRC). The compound yields inhibitory effects on Candida albicans (C. albicans), Staphylococcus aureus (S. aureus), and Escherichia coli (E. coli). Specifically, DMBQ disrupts the cell membrane structure of fungi and bacteria, alters membrane permeability, and induces intracellular reactive oxygen species (ROS) accumulation. In addition, DMBQ effectively suppresses the biosynthesis of fungal ergosterol and bacterial fatty acids. The synergistic action of these multiple mechanisms contributes to the antimicrobial effects of DMBQ. We further fabricated DMBQ-loaded photocurable hydrogel microneedles, HA-GMA-DMBQ MNs (HGD MNs). The covalently cross-linked network of HGD MNs boosts mechanical strength, allowing efficient stratum corneum penetration. HGD MNs exert certain in vitro antimicrobial activity, regulate the wound inflammatory microenvironment in vivo, promote CD31-mediated angiogenesis, and accelerate infected wound healing. Collectively, this work identifies DMBQ as a natural antimicrobial agent, and its combination with hydrogel microneedles offers an effective route for swift therapy of infected wounds.<\/jats:p>","DOI":"10.1021\/acsami.6c10793","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:03:14Z","timestamp":1790787794000},"page":"54432-54455","source":"Crossref","is-referenced-by-count":0,"title":["Identification of a Benzoquinone Antimicrobial from\n                    <i>Ilicis Rotundae Cortex<\/i>\n                    and Its Microneedle-Based Delivery for Infected Wound Healing"],"prefix":"10.1021","volume":"18","author":[{"given":"Wei","family":"Pan","sequence":"first","affiliation":[{"name":"Qingdao University , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Qingdao University , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengyuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Qingdao University , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengjie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Qingdao University , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Rafiq","sequence":"additional","affiliation":[{"name":"Qingdao University , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Yu","sequence":"additional","affiliation":[{"name":"Qingdao University , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0586-3337","authenticated-orcid":true,"given":"Hailin","family":"Cong","sequence":"additional","affiliation":[{"name":"Qingdao University , , ,","place":["Qingdao, China, 266071"]},{"name":"School of Materials Science and Engineering, Shandong University of Technology , ,","place":["Zibo, China, 255000"]},{"name":"Zaozhuang University , , ,","place":["Zaozhuang, China, 277160"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"2026101404075288600_cit1","doi-asserted-by":"publisher","first-page":"3495","DOI":"10.1021\/acs.chemrev.0c00922","article-title":"Ethnobotany and the role of plant natural products in antibiotic drug discovery","volume":"121","author":"Porras","year":"2021","journal-title":"Chem. Rev."},{"key":"2026101404075288600_cit2","doi-asserted-by":"publisher","first-page":"5265","DOI":"10.1039\/c5cs00025d","article-title":"Engineered biosynthesis of natural products in heterologous hosts","volume":"44","author":"Luo","year":"2015","journal-title":"Chem. Soc. Rev."},{"key":"2026101404075288600_cit3","doi-asserted-by":"publisher","first-page":"e30","DOI":"10.1016\/S1473-3099(16)30323-1","article-title":"Potential of marine natural products against drug-resistant fungal, viral, and parasitic infections","volume":"17","author":"Abdelmohsen","year":"2017","journal-title":"Lancet Infect. Dis."},{"key":"2026101404075288600_cit4","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1126\/science.aay9173","article-title":"Total synthesis of the complex taxane diterpene canataxpropellane","volume":"367","author":"Schneider","year":"2020","journal-title":"Science"},{"key":"2026101404075288600_cit5","doi-asserted-by":"publisher","DOI":"10.1126\/science.adk5382","article-title":"Artemisinins ameliorate polycystic ovarian syndrome by mediating LONP1-CYP11A1 interaction","volume":"384","author":"Liu","year":"2024","journal-title":"Science"},{"key":"2026101404075288600_cit6","doi-asserted-by":"publisher","first-page":"1130","DOI":"10.1038\/s41565-021-00950-z","article-title":"Immunogenic camptothesome nanovesicles comprising sphingomyelin-derived camptothecin bilayers for safe and synergistic cancer immunochemotherapy","volume":"16","author":"Wang","year":"2021","journal-title":"Nat. Nanotechnol."},{"key":"2026101404075288600_cit7","doi-asserted-by":"publisher","first-page":"1790","DOI":"10.1126\/science.1058092","article-title":"Biosynthesis of complex polyketides in a metabolically engineered strain of E coli","volume":"291","author":"Pfeifer","year":"2001","journal-title":"Science"},{"key":"2026101404075288600_cit8","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1038\/s41586-022-05157-3","article-title":"A microbial supply chain for production of the anti-cancer drug vinblastine","volume":"609","author":"Zhang","year":"2022","journal-title":"Nature"},{"key":"2026101404075288600_cit9","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1021\/acs.jnatprod.5c01368","article-title":"The role of natural product chemistry in drug discovery: Two decades of progress and perspectives","volume":"89","author":"Butler","year":"2026","journal-title":"J. Nat. Prod."},{"key":"2026101404075288600_cit10","doi-asserted-by":"publisher","first-page":"3434","DOI":"10.1021\/acs.jmedchem.5c03455","article-title":"Discovery and structural optimization of spirodioxynaphthalene-based Mdr1 efflux pump inhibitor to combat azole resistance in Candida albicans","volume":"69","author":"Zhang","year":"2026","journal-title":"J. Med. Chem."},{"key":"2026101404075288600_cit11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2021\/9570776","article-title":"Rapid identification of the chemical components of Ilex rotunda Thunb using UPLC-Q-TOF-MS\/MS","volume":"2021","author":"Chen","year":"2021","journal-title":"J. Chem."},{"key":"2026101404075288600_cit12","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2019.152941","article-title":"Extract of Ilex rotunda Thunb alleviates experimental colitis-associated cancer via suppressing inflammation-induced miR-31-5p\/YAP overexpression","volume":"62","author":"Chen","year":"2019","journal-title":"Phytomedicine"},{"key":"2026101404075288600_cit13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2022.115419","article-title":"A systematic review: Botany, phytochemistry, traditional uses, pharmacology, toxicology, quality control and pharmacokinetics of Ilex rotunda Thunb","volume":"298","author":"Zeng","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"2026101404075288600_cit14","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2022.109610","article-title":"Anti-inflammatory activity of caffeic acid derivatives from Ilex rotunda","volume":"115","author":"Kim","year":"2023","journal-title":"Int. Immunopharmacol."},{"key":"2026101404075288600_cit15","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1080\/13880209.2017.1279673","article-title":"Antibacterial activities of chemical constituents from the aerial parts of Hedyotis pilulifera","volume":"55","author":"Nguyen","year":"2017","journal-title":"Pharm. Biol."},{"key":"2026101404075288600_cit16","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/s41589-022-01040-4","article-title":"Expanding the search for small-molecule antibacterials by multidimensional profiling","volume":"18","author":"Ortmayr","year":"2022","journal-title":"Nat. Chem. Biol."},{"key":"2026101404075288600_cit17","doi-asserted-by":"publisher","first-page":"3420","DOI":"10.1038\/s41467-025-58804-4","article-title":"Pre-trained molecular representations enable antimicrobial discovery","volume":"16","author":"Olayo-Alarcon","year":"2025","journal-title":"Nat. Commun."},{"key":"2026101404075288600_cit18","doi-asserted-by":"publisher","first-page":"726","DOI":"10.1038\/s41570-021-00313-1","article-title":"Towards the sustainable discovery and development of new antibiotics","volume":"5","author":"Miethke","year":"2021","journal-title":"Nat. Rev. Chem."},{"key":"2026101404075288600_cit19","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202503517","article-title":"Unilateral surface-crystal-engineering induced dual-bionic janus multifunctional wound dressing for infected burn wound healing","volume":"35","author":"Lv","year":"2025","journal-title":"Adv. Funct. Mater."},{"key":"2026101404075288600_cit20","doi-asserted-by":"publisher","first-page":"13022","DOI":"10.1021\/acsnano.2c05557","article-title":"Bacterial growth-induced tobramycin smart release self-healing hydrogel for Pseudomonas aeruginosa-infected burn wound healing","volume":"16","author":"Huang","year":"2022","journal-title":"ACS Nano"},{"key":"2026101404075288600_cit21","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1016\/j.bioactmat.2023.03.005","article-title":"Microenvironment responsive nanocomposite hydrogel with NIR photothermal therapy, vascularization and anti-inflammation for diabetic infected wound healing","volume":"26","author":"Zhu","year":"2023","journal-title":"Bioact. Mater."},{"key":"2026101404075288600_cit22","doi-asserted-by":"publisher","first-page":"1102","DOI":"10.1126\/science.abg6017","article-title":"Fungi prevent intestinal healing","volume":"371","author":"Chiaro","year":"2021","journal-title":"Science"},{"key":"2026101404075288600_cit23","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1186\/s12951-024-02390-y","article-title":"Construction of programmed time-released multifunctional hydrogel with antibacterial and anti-inflammatory properties for impaired wound healing","volume":"22","author":"Peng","year":"2024","journal-title":"J. Nanobiotechnol."},{"key":"2026101404075288600_cit24","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2025.123432","article-title":"AIE-active PVA\/berberine antibacterial hydrogel for wound healing, visual monitoring pH and dehydration","volume":"323","author":"Gu","year":"2025","journal-title":"Biomaterials"},{"key":"2026101404075288600_cit25","doi-asserted-by":"publisher","DOI":"10.1016\/j.cej.2025.162544","article-title":"A near-infrared responsive hydrogel loaded with Prussian blue-based nanocarriers for CO gas therapy of infected wounds","volume":"512","author":"Li","year":"2025","journal-title":"Chem. Eng. J."},{"key":"2026101404075288600_cit26","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202404813","article-title":"Calcium peroxide-based hydrogels enable biphasic release of hydrogen peroxide for infected wound healing","volume":"11","author":"Huang","year":"2024","journal-title":"Adv. Sci."},{"key":"2026101404075288600_cit27","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1038\/s41579-024-01035-z","article-title":"The wound microbiota: microbial mechanisms of impaired wound healing and infection","volume":"22","author":"Uberoi","year":"2024","journal-title":"Nat. Rev. Microbiol."},{"key":"2026101404075288600_cit28","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201900764","article-title":"Bioinorganics and wound healing","volume":"8","author":"Dalisson","year":"2019","journal-title":"Adv. Healthc. Mater."},{"key":"2026101404075288600_cit29","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202214454","article-title":"In situ enzyme-induced self-assembly of antimicrobial-antioxidative peptides to promote wound healing","volume":"33","author":"Teng","year":"2023","journal-title":"Adv. Funct. Mater."},{"key":"2026101404075288600_cit30","doi-asserted-by":"publisher","first-page":"1784","DOI":"10.1016\/j.apsb.2019.12.014","article-title":"Self-assembled natural phytochemicals for synergistically antibacterial application from the enlightenment of traditional Chinese medicine combination","volume":"10","author":"Tian","year":"2020","journal-title":"Acta Pharm. Sin. B"},{"key":"2026101404075288600_cit31","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2024.215907","article-title":"Recent advances in NIR-II fluorescence\/photoacoustic dual-modality imaging probes","volume":"514","author":"Pan","year":"2024","journal-title":"Coord. Chem. Rev."},{"key":"2026101404075288600_cit32","doi-asserted-by":"publisher","first-page":"3507","DOI":"10.1016\/j.bioactmat.2021.03.023","article-title":"Chinese herb microneedle patch for wound healing","volume":"6","author":"Chi","year":"2021","journal-title":"Bioact. Mater."},{"key":"2026101404075288600_cit33","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202307585","article-title":"Chlorella-loaded antibacterial microneedles for microacupuncture oxygen therapy of diabetic bacterial infected wounds","volume":"36","author":"Gao","year":"2024","journal-title":"Adv. Mater."},{"key":"2026101404075288600_cit34","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202316565","article-title":"Rolling stone gathers moss: Rolling microneedles generate meta microfluidic microneedles (MMM)","volume":"34","author":"Zhou","year":"2024","journal-title":"Adv. Funct. Mater."},{"key":"2026101404075288600_cit35","doi-asserted-by":"publisher","first-page":"8336","DOI":"10.1007\/s12274-022-4508-y","article-title":"Rapidly separable bubble microneedle patch for effective local anesthesia","volume":"15","author":"Yang","year":"2022","journal-title":"Nano Res."},{"key":"2026101404075288600_cit36","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202501015","article-title":"Microneedle-enabled breakthroughs in nucleic acid therapeutics","volume":"14","author":"Wu","year":"2025","journal-title":"Adv. Healthc. Mater."},{"key":"2026101404075288600_cit37","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1016\/j.jconrel.2022.06.021","article-title":"Research progress of microneedles in the treatment of melanoma","volume":"348","author":"Li","year":"2022","journal-title":"J. Control. Release"},{"key":"2026101404075288600_cit38","doi-asserted-by":"publisher","first-page":"1008","DOI":"10.1038\/s41551-021-00720-1","article-title":"Cryomicroneedles for transdermal cell delivery","volume":"5","author":"Chang","year":"2021","journal-title":"Nat. Biomed. Eng."},{"key":"2026101404075288600_cit39","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202402009","article-title":"Multifunctional dopamine-based hydrogel microneedle electrode for continuous ketone sensing","volume":"36","author":"Ausri","year":"2024","journal-title":"Adv. Mater."},{"key":"2026101404075288600_cit40","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202417774","article-title":"Amyloid-templated ceria nanozyme reinforced microneedle for diabetic wound treatments","volume":"37","author":"Xuan","year":"2025","journal-title":"Adv. Mater."},{"key":"2026101404075288600_cit41","doi-asserted-by":"publisher","first-page":"26733","DOI":"10.1021\/acsnano.4c06921","article-title":"Hydrogel microneedle patches loaded with stem cell mitochondria-enriched microvesicles boost the chronic wound healing","volume":"18","author":"Yao","year":"2024","journal-title":"ACS Nano"},{"key":"2026101404075288600_cit42","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202210903","article-title":"Bioinspired adaptable indwelling microneedles for treatment of diabetic ulcers","volume":"35","author":"Zhang","year":"2023","journal-title":"Adv. Mater."},{"key":"2026101404075288600_cit43","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2025.123134","article-title":"Recent advances in smart hydrogels derived from polysaccharides and their applications for wound dressing and healing","volume":"318","author":"Tian","year":"2025","journal-title":"Biomaterials"},{"key":"2026101404075288600_cit44","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120602","article-title":"Hyaluronic acid and chondroitin sulfate (meth)acrylate-based hydrogels for tissue engineering: Synthesis, characteristics and pre-clinical evaluation","volume":"268","author":"Schuurmans","year":"2021","journal-title":"Biomaterials"},{"key":"2026101404075288600_cit45","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.bioactmat.2022.02.015","article-title":"Bottlebrush inspired injectable hydrogel for rapid prevention of postoperative and recurrent adhesion","volume":"16","author":"Gao","year":"2022","journal-title":"Bioact. Mater."}],"container-title":["ACS Applied Materials &amp; Interfaces"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsami.6c10793","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/18\/40\/54432\/68715607\/am-2026-10793f.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/18\/40\/54432\/68715607\/am-2026-10793f.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T08:29:26Z","timestamp":1791966566000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aamick\/article\/18\/40\/54432\/5442642\/Identification-of-a-Benzoquinone-Antimicrobial"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":45,"journal-issue":{"issue":"40","published-online":{"date-parts":[[2026,9,30]]},"published-print":{"date-parts":[[2026,10,14]]}},"URL":"https:\/\/doi.org\/10.1021\/acsami.6c10793","relation":{},"ISSN":["1944-8244","1944-8252"],"issn-type":[{"value":"1944-8244","type":"print"},{"value":"1944-8252","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]}}}