{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T08:37:20Z","timestamp":1793176640604,"version":"4.5.0"},"reference-count":58,"publisher":"American Chemical Society (ACS)","issue":"42","license":[{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:00:00Z","timestamp":1792108800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100007859","name":"University of Kansas","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007859","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Delivery of CRISPR-Cas9 ribonucleoprotein (RNP) using synthetic materials can bypass the host translational machinery and limit the duration of genomic exposure, minimizing the risk of off-target mutations. Despite their advantages, lipid nanoparticle (LNP) platforms for CRISPR-Cas9 RNP delivery that achieve highly efficient editing at low doses remain limited. In the present study, we developed a library of ionizable lipids (ILs) and corresponding LNPs to enhance the delivery and editing efficiencies of CRISPR-Cas9 RNP complexes. Following comprehensive in vitro screening and formulation optimization, an optimal LNP candidate containing the C12A9 IL (denoted as LNPC12A9) was identified that achieved \u223c90% EGFP disruption in HEK293T-EGFP cells at an RNP concentration of 30 nM and \u223c60% at 15 nM. The editing performance was consistently observed at an RNP concentration of 15 nM when editing the Gcgr and HPRT1 genes across multiple hepatic cell lines. Moreover, coencapsulation of RNP and a single-stranded oligodeoxynucleotide (ssODN) template for homology-directed repair (HDR) within LNPC12A9 induced an HDR rate of 37.8%. Systemic administration of LNPC12A9 into C57BL\/6J mice led to liver tropism and a total hepatic Pcsk9 gene disruption rate of 1.39%. In conclusion, this study provides insights into the construction of an LNP platform to improve the delivery efficiency of CRISPR-Cas9 RNP, thereby facilitating the development of LNPs for safe, precise, and tissue-specific Cas9 RNP-based genome editing therapies.<\/jats:p>","DOI":"10.1021\/acsami.6c11615","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T15:01:49Z","timestamp":1792162909000},"page":"57043-57056","source":"Crossref","is-referenced-by-count":0,"title":["High-Throughput Screening of a Lipid Nanoparticle (LNP) Library Identifies Lead Formulations for CRISPR-Cas9 Ribonucleoprotein Delivery and Gene Editing"],"prefix":"10.1021","volume":"18","author":[{"given":"Yu","family":"Wang","sequence":"first","affiliation":[{"name":"University of Kansas , , , ,","place":["Lawrence, Kansas, United States, 66047"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9035-5050","authenticated-orcid":true,"given":"Amin","family":"Haghighat Naeini","sequence":"additional","affiliation":[{"name":"University of Kansas , , , ,","place":["Lawrence, Kansas, United States, 66047"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishant","family":"Kumar","sequence":"additional","affiliation":[{"name":"University of Kansas , , , ,","place":["Lawrence, Kansas, United States, 66047"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2704-8036","authenticated-orcid":true,"given":"Ming","family":"Zhao","sequence":"additional","affiliation":[{"name":"University of Kansas , , , ,","place":["Lawrence, Kansas, United States, 66047"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,16]]},"reference":[{"issue":"2","key":"2026102801063927400_cit1","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1016\/j.ymthe.2020.09.028","article-title":"CRISPR-Cas9: A Preclinical and Clinical Perspective for the Treatment of Human Diseases","volume":"29","author":"Sharma","year":"2021","journal-title":"Mol. Ther."},{"issue":"7601","key":"2026102801063927400_cit2","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1038\/nature17664","article-title":"Efficient introduction of specific homozygous and heterozygous mutations using CRISPR\/Cas9","volume":"533","author":"Paquet","year":"2016","journal-title":"Nature"},{"issue":"5","key":"2026102801063927400_cit3","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1089\/crispr.2024.0042","article-title":"Affordable Pricing of CRISPR Treatments is a Pressing Ethical Imperative","volume":"7","author":"Rueda","year":"2024","journal-title":"CRISPR J."},{"issue":"6","key":"2026102801063927400_cit4","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1038\/s44161-022-00085-x","article-title":"Chasing LDL cholesterol to the bottom - PCSK9 in perspective","volume":"1","author":"Libby","year":"2022","journal-title":"Nat. Cardiovasc. Res."},{"issue":"10","key":"2026102801063927400_cit5","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1038\/nrendo.2017.80","article-title":"Regulation of hepatic glucose metabolism in health and disease","volume":"13","author":"Petersen","year":"2017","journal-title":"Nat. Rev. Endocrinol."},{"issue":"6737","key":"2026102801063927400_cit6","doi-asserted-by":"publisher","DOI":"10.1126\/science.adp4120","article-title":"Liver ALKBH5 regulates glucose and lipid homeostasis independently through GCGR and mTORC1 signaling","volume":"387","author":"Ding","year":"2025","journal-title":"Science"},{"issue":"1","key":"2026102801063927400_cit7","doi-asserted-by":"publisher","first-page":"21474","DOI":"10.1038\/s41598-020-78450-8","article-title":"Efficient viral delivery of Cas9 into human safe harbor","volume":"10","author":"Hayashi","year":"2020","journal-title":"Sci. Rep."},{"issue":"1","key":"2026102801063927400_cit8","doi-asserted-by":"publisher","first-page":"3497","DOI":"10.1038\/s41467-018-05843-9","article-title":"Cas9 immunity creates challenges for CRISPR gene editing therapies","volume":"9","author":"Crudele","year":"2018","journal-title":"Nat. Commun."},{"key":"2026102801063927400_cit9","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1038\/s41551-017-0137-2","article-title":"Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair","volume":"1","author":"Lee","year":"2017","journal-title":"Nat. Biomed. Eng."},{"issue":"10","key":"2026102801063927400_cit10","doi-asserted-by":"publisher","first-page":"974","DOI":"10.1038\/s41565-019-0539-2","article-title":"A biodegradable nanocapsule delivers a Cas9 ribonucleoprotein complex for in vivo genome editing","volume":"14","author":"Chen","year":"2019","journal-title":"Nat. Nanotechnol."},{"issue":"12","key":"2026102801063927400_cit11","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aay3255","article-title":"Carboxylated branched poly(\u03b2-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing","volume":"5","author":"Rui","year":"2019","journal-title":"Sci. Adv."},{"key":"2026102801063927400_cit12","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.jconrel.2022.02.035","article-title":"Rationally designed nanoparticle delivery of Cas9 ribonucleoprotein for effective gene editing","volume":"345","author":"Chae","year":"2022","journal-title":"J. Controlled Release"},{"issue":"1","key":"2026102801063927400_cit13","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1021\/jacs.7b11754","article-title":"Endosomal Escape and Delivery of CRISPR\/Cas9 Genome Editing Machinery Enabled by Nanoscale Zeolitic Imidazolate Framework","volume":"140","author":"Alsaiari","year":"2018","journal-title":"J. Am. Chem. Soc."},{"issue":"47","key":"2026102801063927400_cit14","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abc9450","article-title":"CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy","volume":"6","author":"Rosenblum","year":"2020","journal-title":"Sci. Adv."},{"issue":"36","key":"2026102801063927400_cit15","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202502540","article-title":"Lipid Nanoparticles for the Delivery of CRISPR\/Cas9 Machinery to Enable Site-Specific Integration of CFTR and Mutation-Agnostic Disease Rescue","volume":"36","author":"Foley","year":"2026","journal-title":"Adv. Funct. Mater."},{"key":"2026102801063927400_cit16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2025.114499","article-title":"Lipid nanoparticle-mediated CRISPR\/Cas9 delivery enables efficient trabecular meshwork gene editing in mice","volume":"389","author":"Huang","year":"2026","journal-title":"J. Controlled Release"},{"issue":"31","key":"2026102801063927400_cit17","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202402259","article-title":"A Multifunctional Aptamer Decorated Lipid Nanoparticles for the Delivery of EpCAM-targeted CRISPR\/Cas9 Plasmid for Efficacious In Vivo Tumor Regression","volume":"13","author":"Sarkar","year":"2024","journal-title":"Adv. Healthcare Mater."},{"issue":"36","key":"2026102801063927400_cit18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2426094122","article-title":"A general genome editing strategy using CRISPR lipid nanoparticle spherical nucleic acids","volume":"122","author":"Han","year":"2025","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"14","key":"2026102801063927400_cit19","doi-asserted-by":"publisher","first-page":"13790","DOI":"10.1021\/acsnano.4c16617","article-title":"Lipid Nanoparticles for In Vivo Lung Delivery of CRISPR-Cas9 Ribonucleoproteins Allow Gene Editing of Clinical Targets","volume":"19","author":"Haley","year":"2025","journal-title":"ACS Nano"},{"issue":"35","key":"2026102801063927400_cit20","doi-asserted-by":"publisher","first-page":"45871","DOI":"10.1021\/acsami.4c04893","article-title":"Antisense Oligonucleotide Embedded Context Responsive Nanoparticles Derived from Synthetic Ionizable Lipids for lncRNA Targeted Therapy of Breast Cancer","volume":"16","author":"Sarkar","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"1","key":"2026102801063927400_cit21","doi-asserted-by":"publisher","first-page":"7233","DOI":"10.1038\/s41467-021-27493-0","article-title":"An ionizable lipid toolbox for RNA delivery","volume":"12","author":"Han","year":"2021","journal-title":"Nat. Commun."},{"issue":"1","key":"2026102801063927400_cit22","doi-asserted-by":"publisher","first-page":"5659","DOI":"10.1038\/s41467-024-50093-7","article-title":"Reformulating lipid nanoparticles for organ-targeted mRNA accumulation and translation","volume":"15","author":"Su","year":"2024","journal-title":"Nat. Commun."},{"issue":"40","key":"2026102801063927400_cit23","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202507657","article-title":"Reengineering Endogenous Targeting Lipid Nanoparticles (ENDO) for Systemic Delivery of mRNA to Pancreas","volume":"37","author":"Isaac","year":"2025","journal-title":"Adv. Mater."},{"issue":"31","key":"2026102801063927400_cit24","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202400493","article-title":"Lipid Nanoparticle-Mediated Delivery of CRISPR-Cas9 Against Rubicon Ameliorates NAFLD by Modulating CD36 Along with Glycerophospholipid Metabolism","volume":"11","author":"Bai","year":"2024","journal-title":"Adv. Sci."},{"issue":"28","key":"2026102801063927400_cit25","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adj0006","article-title":"Dynamically covalent lipid nanoparticles mediate CRISPR-Cas9 genome editing against choroidal neovascularization in mice","volume":"11","author":"Cao","year":"2025","journal-title":"Sci. Adv."},{"issue":"2","key":"2026102801063927400_cit26","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e24606","article-title":"Lipid nanoparticles: The game-changer in CRISPR-Cas9 genome editing","volume":"10","author":"Mohammadian Farsani","year":"2024","journal-title":"Heliyon"},{"issue":"2","key":"2026102801063927400_cit27","doi-asserted-by":"publisher","first-page":"614","DOI":"10.7150\/thno.47007","article-title":"Strategies in the delivery of Cas9 ribonucleoprotein for CRISPR\/Cas9 genome editing","volume":"11","author":"Zhang","year":"2021","journal-title":"Theranostics"},{"key":"2026102801063927400_cit28","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1016\/j.jbiotec.2015.04.024","article-title":"Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection","volume":"208","author":"Liang","year":"2015","journal-title":"J. Biotechnol."},{"issue":"5","key":"2026102801063927400_cit29","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf105","article-title":"Packaged delivery of CRISPR-Cas9 ribonucleoproteins accelerates genome editing","volume":"53","author":"Karp","year":"2025","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"2026102801063927400_cit30","doi-asserted-by":"publisher","first-page":"3232","DOI":"10.1038\/s41467-020-17029-3","article-title":"Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing","volume":"11","author":"Wei","year":"2020","journal-title":"Nat. Commun."},{"issue":"6","key":"2026102801063927400_cit31","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.201800996","article-title":"Intracellular Delivery of His-Tagged Genome-Editing Proteins Enabled by Nitrilotriacetic Acid-Containing Lipidoid Nanoparticles","volume":"8","author":"Li","year":"2019","journal-title":"Adv. Healthcare Mater."},{"issue":"11","key":"2026102801063927400_cit32","doi-asserted-by":"publisher","first-page":"2868","DOI":"10.1073\/pnas.1520244113","article-title":"Efficient delivery of genome-editing proteins using bioreducible lipid nanoparticles","volume":"113","author":"Wang","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"9","key":"2026102801063927400_cit33","doi-asserted-by":"publisher","first-page":"1445","DOI":"10.1038\/s41587-024-02437-3","article-title":"Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 ribonucleoprotein","volume":"43","author":"Chen","year":"2025","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"2026102801063927400_cit34","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1038\/s41551-024-01296-2","article-title":"Safer and efficient base editing and prime editing via ribonucleoproteins delivered through optimized lipid-nanoparticle formulations","volume":"9","author":"Ho\u0142ubowicz","year":"2025","journal-title":"Nat. Biomed. Eng."},{"issue":"1","key":"2026102801063927400_cit35","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1038\/s41467-024-55137-6","article-title":"Branched endosomal disruptor (BEND) lipids mediate delivery of mRNA and CRISPR-Cas9 ribonucleoprotein complex for hepatic gene editing and T cell engineering","volume":"16","author":"Padilla","year":"2025","journal-title":"Nat. Commun."},{"key":"2026102801063927400_cit36","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1339189","article-title":"Beyond the promise: evaluating and mitigating off-target effects in CRISPR gene editing for safer therapeutics","volume":"11","author":"Lopes","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"2026102801063927400_cit37","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.addr.2015.09.012","article-title":"PEGylation as a strategy for improving nanoparticle-based drug and gene delivery","volume":"99","author":"Suk","year":"2016","journal-title":"Adv. Drug Delivery Rev."},{"key":"2026102801063927400_cit38","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.jconrel.2025.01.071","article-title":"Role of PEGylated lipid in lipid nanoparticle formulation for and delivery of mRNA vaccines","volume":"380","author":"Zhang","year":"2025","journal-title":"J. Controlled Release"},{"key":"2026102801063927400_cit39","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1016\/j.jconrel.2023.02.008","article-title":"Lipid nanoparticle-based ribonucleoprotein delivery for in vivo genome editing","volume":"355","author":"Onuma","year":"2023","journal-title":"J. Controlled Release"},{"issue":"4","key":"2026102801063927400_cit40","doi-asserted-by":"publisher","first-page":"961","DOI":"10.1091\/mbc.10.4.961","article-title":"Extraction of cholesterol with methyl-\u03b2-cyclodextrin perturbs formation of clathrin-coated endocytic vesicles","volume":"10","author":"Rodal","year":"1999","journal-title":"Mol. Biol. Cell"},{"issue":"5","key":"2026102801063927400_cit41","doi-asserted-by":"publisher","DOI":"10.1210\/jendso\/bvac030","article-title":"The Role of Lipid Rafts and Membrane Androgen Receptors in Androgen's Neurotoxic Effects","volume":"6","author":"Fadeyibi","year":"2022","journal-title":"J. Endocr. Soc."},{"issue":"10","key":"2026102801063927400_cit42","doi-asserted-by":"publisher","first-page":"2092","DOI":"10.1038\/s41591-022-02011-x","article-title":"Glucagon receptor antagonist volagidemab in type 1 diabetes: a 12-week, randomized, double-blind, phase 2 trial","volume":"28","author":"Pettus","year":"2022","journal-title":"Nat. Med."},{"issue":"7859","key":"2026102801063927400_cit43","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1038\/s41586-021-03534-y","article-title":"In vivo CRISPR base editing of PCSK9 durably lowers cholesterol in primates","volume":"593","author":"Musunuru","year":"2021","journal-title":"Nature"},{"key":"2026102801063927400_cit44","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpx.2024.100305","article-title":"Exploring the landscape of Lipid Nanoparticles (LNPs): A comprehensive review of LNPs types and biological sources of lipids","volume":"8","author":"Alfutaimani","year":"2024","journal-title":"Int. J. Pharm. X"},{"issue":"15","key":"2026102801063927400_cit45","doi-asserted-by":"publisher","first-page":"1625","DOI":"10.1007\/s40265-018-0983-6","article-title":"Patisiran: First Global Approval","volume":"78","author":"Hoy","year":"2018","journal-title":"Drugs"},{"issue":"6","key":"2026102801063927400_cit46","doi-asserted-by":"publisher","first-page":"947","DOI":"10.1177\/08971900211009650","article-title":"Review of COVID-19 mRNA Vaccines: BNT162b2 and mRNA-1273","volume":"35","author":"Teo","year":"2022","journal-title":"J. Pharm. Pract."},{"issue":"9","key":"2026102801063927400_cit47","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1038\/s41573-024-00977-6","article-title":"The 60-year evolution of lipid nanoparticles for nucleic acid delivery","volume":"23","author":"Cullis","year":"2024","journal-title":"Nat. Rev. Drug Discovery"},{"issue":"1","key":"2026102801063927400_cit48","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14010213","article-title":"Impact of Formulation Conditions on Lipid Nanoparticle Characteristics and Functional Delivery of CRISPR RNP for Gene Knock-Out and Correction","volume":"14","author":"Ho\u0142ubowicz","year":"2022","journal-title":"Pharmaceutics"},{"issue":"8045","key":"2026102801063927400_cit49","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1038\/s41586-024-08291-2","article-title":"Placenta-tropic VEGF mRNA lipid nanoparticles ameliorate murine pre-eclampsia","volume":"637","author":"Swingle","year":"2025","journal-title":"Nature"},{"issue":"4","key":"2026102801063927400_cit50","doi-asserted-by":"publisher","first-page":"1334","DOI":"10.1016\/j.biomaterials.2013.10.062","article-title":"A cationic cholesterol based nanocarrier for the delivery of p53-EGFP-C3 plasmid to cancer cells","volume":"35","author":"Misra","year":"2014","journal-title":"Biomaterials"},{"issue":"5","key":"2026102801063927400_cit51","doi-asserted-by":"publisher","first-page":"1222","DOI":"10.1016\/j.bbamem.2007.12.010","article-title":"Effect of the headgroup variation on the gene transfer properties of cholesterol based cationic lipids possessing ether linkage","volume":"1778","author":"Bajaj","year":"2008","journal-title":"Biochim. Biophys. Acta, Biomembr."},{"issue":"11","key":"2026102801063927400_cit52","doi-asserted-by":"publisher","first-page":"3951","DOI":"10.1021\/bm401079h","article-title":"Effects of a delocalizable cation on the headgroup of gemini lipids on the lipoplex-type nanoaggregates directly formed from plasmid DNA","volume":"14","author":"Misra","year":"2013","journal-title":"Biomacromolecules"},{"issue":"5","key":"2026102801063927400_cit53","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2409572122","article-title":"Rational design and modular synthesis of biodegradable ionizable lipids via the Passerini reaction for mRNA delivery","volume":"122","author":"Xu","year":"2025","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026102801063927400_cit54","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/j.jconrel.2020.03.006","article-title":"Different kinetics for the hepatic uptake of lipid nanoparticles between the apolipoprotein E\/low density lipoprotein receptor and the N-acetyl-D-galactosamine\/asialoglycoprotein receptor pathway","volume":"322","author":"Sato","year":"2020","journal-title":"J. Controlled Release"},{"issue":"4","key":"2026102801063927400_cit55","doi-asserted-by":"publisher","first-page":"1943","DOI":"10.1016\/j.apsb.2025.11.023","article-title":"Deciphering the biological fate of mRNA-LNP-based biologics: A perspective from tissue to intracellular distribution","volume":"16","author":"Xu","year":"2026","journal-title":"Acta Pharm. Sin. B"},{"issue":"4","key":"2026102801063927400_cit56","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1038\/s41565-020-0669-6","article-title":"Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR-Cas gene editing","volume":"15","author":"Cheng","year":"2020","journal-title":"Nat. Nanotechnol."},{"issue":"2","key":"2026102801063927400_cit57","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1038\/s41551-022-00847-9","article-title":"Augmented lipid-nanoparticle-mediated in vivo genome editing in the lungs and spleen by disrupting Cas9 activity in the liver","volume":"6","author":"Sago","year":"2022","journal-title":"Nat. Biomed. Eng."},{"issue":"1","key":"2026102801063927400_cit58","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1038\/nbt.3081","article-title":"Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo","volume":"33","author":"Zuris","year":"2015","journal-title":"Nat. Biotechnol."}],"container-title":["ACS Applied Materials &amp; Interfaces"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsami.6c11615","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/18\/42\/57043\/68886614\/am-2026-11615u.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/18\/42\/57043\/68886614\/am-2026-11615u.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T08:11:32Z","timestamp":1793175092000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aamick\/article\/18\/42\/57043\/5452764\/High-Throughput-Screening-of-a-Lipid-Nanoparticle"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,16]]},"references-count":58,"journal-issue":{"issue":"42","published-online":{"date-parts":[[2026,10,16]]},"published-print":{"date-parts":[[2026,10,28]]}},"URL":"https:\/\/doi.org\/10.1021\/acsami.6c11615","relation":{},"ISSN":["1944-8244","1944-8252"],"issn-type":[{"value":"1944-8244","type":"print"},{"value":"1944-8252","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,16]]}}}