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In colloidal systems, the measured response is determined not only by analyte concentration, but also by nanoparticle number density, colloidal stability, probe\u2212surface interactions, and the balance between transient and persistent interparticle coupling. Here, we provide evidence that nonlinear SERS amplification in colloidal gold suspensions may arise from two fundamentally different regimes. In stable dispersions, the signal increase would be consistent with the formation of dynamic hot spots associated with Brownian-motion-driven transient near-contact nanoparticle configurations. In contrast, when the molecular probe perturbs colloidal stability, the enhancement becomes dominated by aggregation-induced hot spots, followed by signal loss as aggregation progresses and active clusters precipitation from suspension. These findings suggest that similar intensity trends may arise from distinct physical mechanisms with very different analytical consequences. Distinguishing dynamic from aggregation-induced hot spots is therefore essential for the correct interpretation of colloidal SERS data and for advancing SERS toward robust and reliable quantitation.<\/jats:p>","DOI":"10.1021\/acsami.6c12440","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:01:23Z","timestamp":1792090883000},"source":"Crossref","is-referenced-by-count":0,"title":["Insights into Dynamic Hot Spot Contributions in Quantitative Colloidal SERS"],"prefix":"10.1021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9717-6859","authenticated-orcid":true,"given":"Irene","family":"Calderon Gonzalez","sequence":"first","affiliation":[{"name":"Universitat Rovira i Virgili , , ,","place":["Tarragona, Spain, 43007"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9184-8055","authenticated-orcid":true,"given":"I. Brian","family":"Becerril-Castro","sequence":"additional","affiliation":[{"name":"Universitat Rovira i Virgili , , ,","place":["Tarragona, Spain, 43007"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1950-1414","authenticated-orcid":true,"given":"Miguel A.","family":"Correa-Duarte","sequence":"additional","affiliation":[{"name":"Universidade de Vigo , , ,","place":["Vigo, Spain, 36310"]},{"name":"Southern Galicia Institute of Health Research (IISGS) , ,","place":["Vigo, Spain, 36310"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4770-5756","authenticated-orcid":true,"given":"Ramon A.","family":"Alvarez-Puebla","sequence":"additional","affiliation":[{"name":"Universitat Rovira i Virgili , , ,","place":["Tarragona, Spain, 43007"]},{"name":"ICREA\u2014Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats , ,","place":["Barcelona, Spain, 08010"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"issue":"1","key":"2026101515011462700_cit1","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1021\/acsnano.9b04224","article-title":"Present and Future of Surface-Enhanced Raman Scattering","volume":"14","author":"Langer","year":"2020","journal-title":"ACS Nano"},{"issue":"11","key":"2026101515011462700_cit2","doi-asserted-by":"publisher","first-page":"16287","DOI":"10.1021\/acsami.4c17502","article-title":"Surface-Enhanced Raman Spectroscopy for Biomedical Applications: Recent Advances and Future Challenges","volume":"17","author":"Lin","year":"2025","journal-title":"ACS Appl. 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