{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T03:32:42Z","timestamp":1793331162860,"version":"4.5.0"},"reference-count":30,"publisher":"American Chemical Society (ACS)","issue":"42","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["1925\/23"],"award-info":[{"award-number":["1925\/23"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Diane and Guilford Glazer Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>All-gold electron microscopy (EM) grids reduce beam-induced motion relative to conventional holey carbon supports and provide biocompatible substrates for cellular cryo-EM. However, placing customizable all-gold grid fabrication in the hands of researchers requires accessible processes based on standard microfabrication tools. We report a wafer-scale process using microfabrication techniques available in most academic cleanrooms, such as lift-off metallization, electroplating, and sacrificial layer release, to fabricate 594 all-gold grids per 4-inch wafer without individual grid handling. A numerical electroplating model provides a quantitative framework to relate gold deposition, grid-bar thickness, and tilt-compatible grid geometry. We show that oval 2 \u03bcm \u00d7 6 \u03bcm foil holes bias on-grid actin organization by substrate geometry alone, without chemical micropatterning. The EM grids supported a 2.15 \u00c5 apoferritin single-particle reconstruction on a 200 kV cryo-TEM and are compatible with protein micropatterning and cryo-electron tomography of cells. This platform establishes an accessible route to programmable, application-specific all-gold cryo-EM supports that couple high-resolution structural imaging with engineered control of cellular organization.<\/jats:p>","DOI":"10.1021\/acsami.6c12639","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T23:02:22Z","timestamp":1791500542000},"page":"57081-57090","source":"Crossref","is-referenced-by-count":0,"title":["Gold Electron Microscopy Grids with Anisotropic Foil Geometry Enable On-Grid Contact Guidance"],"prefix":"10.1021","volume":"18","author":[{"given":"Amit","family":"Avrahami","sequence":"first","affiliation":[{"name":"Technion \u2013 Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noa","family":"Ben Asher","sequence":"additional","affiliation":[{"name":"Technion \u2013 Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Zalk","sequence":"additional","affiliation":[{"name":"Ben-Gurion University of the Negev , , ,","place":["Beer Sheva, Israel, 8410501"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5963-8342","authenticated-orcid":true,"given":"Nataliya","family":"Logvina","sequence":"additional","affiliation":[{"name":"Technion \u2013 Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200003"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5329-4300","authenticated-orcid":true,"given":"Leeya","family":"Engel","sequence":"additional","affiliation":[{"name":"Technion \u2013 Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200003"]},{"name":"Technion \u2013 Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200003"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"2026102801070259200_cit1","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1016\/j.jmb.2015.09.030","article-title":"In situ cryo-electron tomography: a post-reductionist approach to structural biology","volume":"428","author":"Asano","year":"2016","journal-title":"J. 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