{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:58:26Z","timestamp":1790758706719,"version":"4.1.0"},"reference-count":70,"publisher":"American Chemical Society (ACS)","issue":"38","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009023","name":"Precursory Research for Embryonic Science and Technology","doi-asserted-by":"publisher","award":["JPJPR19H4"],"award-info":[{"award-number":["JPJPR19H4"]}],"id":[{"id":"10.13039\/501100009023","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23KJ0192"],"award-info":[{"award-number":["23KJ0192"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020964","name":"Fusion Oriented REsearch for disruptive Science and Technology","doi-asserted-by":"publisher","award":["JPMJFR236J"],"award-info":[{"award-number":["JPMJFR236J"]}],"id":[{"id":"10.13039\/501100020964","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Quantitative analysis of small extracellular vesicles (sEVs) at single-particle resolution remains challenging due to their nanoscale dimensions and compositional heterogeneity. Existing methods often rely on specialized and costly instrumentation, limiting accessibility for many researchers and necessitating extensive optimization. Here, we present a porous membrane adsorption for capturing small vesicles (PMACS), which employs a porous anodized aluminum oxide (AAO) membrane as nanoscale compartments to spatially isolate individual vesicles. This allows accessible single-particle discrimination using standard wide-field fluorescence microscopy without either advanced equipment or expert skills. Single-particle discrimination is achieved based on the principle that AAO membranes accommodate vesicles at most one particle per pore according to Poisson distribution and that occupied pores are sufficiently separated beyond the optical resolution limit, which ensures reliable attribution of fluorescent spots to individual vesicles. PMACS achieves reliable quantification of culture cell-derived sEVs across a broad dynamic range (103 to 106 particles\/\u03bcL). This performance exceeds conventional methods without the need for either advanced instrumentation or expert operation. We demonstrate that PMACS allows colocalization analysis of sEV cargos by assessing the tetraspanin (CD9, CD63, and CD81) population of cultured cell-derived sEVs (Hela, A549, and HEK293T cell lines). The heterogeneous protein expressions on sEVs are visualized by means of a simple facile setup. Owing to its simplicity, sensitivity, and accessibility, PMACS provides a practical platform to advance studies of sEV heterogeneity and broaden participation in sEV science.<\/jats:p>","DOI":"10.1021\/acsami.6c12881","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T07:03:02Z","timestamp":1788764582000},"page":"52080-52092","source":"Crossref","is-referenced-by-count":0,"title":["Porous Membrane Adsorption for Capturing Small Extracellular Vesicles Enables Accessible Single-Particle Analysis"],"prefix":"10.1021","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7637-2639","authenticated-orcid":true,"given":"Masahiro","family":"Okada","sequence":"first","affiliation":[{"name":"Tohoku University , , 6-3 Aramaki-Aza Aoba, Aoba-ku , ,","place":["Sendai, Japan, 980-8578"]},{"name":"National Institute of Advanced Industrial Science and Technology (AIST) , 4-2-1, Nigatake, Miyagino-ku , ,","place":["Sendai, Japan, 983-8551"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5824-1654","authenticated-orcid":true,"given":"Yusuke","family":"Sato","sequence":"additional","affiliation":[{"name":"Tohoku University , , 6-3 Aramaki-Aza Aoba, Aoba-ku , ,","place":["Sendai, Japan, 980-8578"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4129-539X","authenticated-orcid":true,"given":"Tetsuji","family":"Itoh","sequence":"additional","affiliation":[{"name":"National Institute of Advanced Industrial Science and Technology (AIST) , 4-2-1, Nigatake, Miyagino-ku , ,","place":["Sendai, Japan, 983-8551"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiichi","family":"Nishizawa","sequence":"additional","affiliation":[{"name":"Tohoku University , , 6-3 Aramaki-Aza Aoba, Aoba-ku , ,","place":["Sendai, Japan, 980-8578"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"issue":"6478","key":"2026093001075885400_cit1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau6977","article-title":"The biology, function, and biomedical applications of exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"issue":"4","key":"2026093001075885400_cit2","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1038\/nrm.2017.125","article-title":"Shedding light on the cell biology of extracellular vesicles","volume":"19","author":"van Niel","year":"2018","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"8","key":"2026093001075885400_cit3","doi-asserted-by":"publisher","first-page":"667","DOI":"10.1016\/j.tcb.2023.01.002","article-title":"Extracellular vesicles and nanoparticles: emerging complexities","volume":"33","author":"Jeppesen","year":"2023","journal-title":"Trends Cell Biol."},{"issue":"1","key":"2026093001075885400_cit4","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1038\/cr.2014.135","article-title":"Discovery of the migrasome, an organelle mediating release of cytoplasmic contents during cell migration","volume":"25","author":"Ma","year":"2015","journal-title":"Cell Res."},{"issue":"23","key":"2026093001075885400_cit5","doi-asserted-by":"publisher","first-page":"3672","DOI":"10.1111\/jcmm.17942","article-title":"Differences between migrasome, a \u2018new organelle\u2019, and exosome","volume":"27","author":"Xu","year":"2023","journal-title":"J. Cell. Mol. Med."},{"issue":"7","key":"2026093001075885400_cit6","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abe5085","article-title":"Mitovesicles are a novel population of extracellular vesicles of mitochondrial origin altered in Down syndrome","volume":"7","author":"D\u2019Acunzo","year":"2021","journal-title":"Sci. Adv."},{"issue":"1","key":"2026093001075885400_cit7","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-025-03354-6","article-title":"Extracellular particles: emerging insights into central nervous system diseases","volume":"23","author":"Chen","year":"2025","journal-title":"J. Nanobiotechnol."},{"issue":"2","key":"2026093001075885400_cit8","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"issue":"6","key":"2026093001075885400_cit9","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1038\/ncb1596","article-title":"Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells","volume":"9","author":"Valadi","year":"2007","journal-title":"Nat. Cell Biol."},{"issue":"4","key":"2026093001075885400_cit10","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1007\/s00109-013-1020-6","article-title":"Exosomes in tumor microenvironment influence cancer progression and metastasis","volume":"91","author":"Kahlert","year":"2013","journal-title":"J. Mol. Med."},{"issue":"7576","key":"2026093001075885400_cit11","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1038\/nature15376","article-title":"Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth","volume":"527","author":"Zhang","year":"2015","journal-title":"Nature"},{"key":"2026093001075885400_cit12","doi-asserted-by":"publisher","first-page":"1451","DOI":"10.1016\/j.biopha.2018.09.174","article-title":"Host derived exosomes-pathogens interactions: Potential functions of exosomes in pathogen infection","volume":"108","author":"Wang","year":"2018","journal-title":"Biomed. Pharmacother."},{"issue":"4","key":"2026093001075885400_cit13","doi-asserted-by":"publisher","first-page":"1178","DOI":"10.1016\/j.bbrc.2016.09.090","article-title":"The role of extracellular vesicles in neurodegenerative diseases","volume":"483","author":"Quek","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"1","key":"2026093001075885400_cit14","doi-asserted-by":"publisher","DOI":"10.1186\/s40779-023-00472-w","article-title":"Mesenchymal stem cell-derived extracellular vesicles in skin wound healing: roles, opportunities and challenges","volume":"10","author":"Ding","year":"2023","journal-title":"Mil. Med. Res."},{"issue":"5","key":"2026093001075885400_cit15","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1038\/s41590-021-00899-0","article-title":"Extracellular vesicles in immunomodulation and tumor progression","volume":"22","author":"Marar","year":"2021","journal-title":"Nat. Immunol."},{"issue":"12","key":"2026093001075885400_cit16","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1038\/s41568-020-00299-w","article-title":"The evolving translational potential of small extracellular vesicles in cancer","volume":"20","author":"M\u00f6ller","year":"2020","journal-title":"Nat. Rev. Cancer"},{"issue":"2","key":"2026093001075885400_cit17","doi-asserted-by":"publisher","DOI":"10.1117\/1.NPh.9.2.021903","article-title":"Overview of extracellular vesicle characterization techniques and introduction to combined reflectance and fluorescence confocal microscopy to distinguish extracellular vesicle subpopulations","volume":"9","author":"Canan","year":"2022","journal-title":"Neurophotonics"},{"issue":"7","key":"2026093001075885400_cit18","doi-asserted-by":"publisher","first-page":"11753","DOI":"10.1021\/acsnano.1c02556","article-title":"Multifluorescence Single Extracellular Vesicle Analysis by Time-Sequential Illumination and Tracking","volume":"15","author":"Cho","year":"2021","journal-title":"ACS Nano"},{"issue":"4","key":"2026093001075885400_cit19","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12206","article-title":"Analysis of extracellular vesicle DNA at the single-vesicle level by nano-flow cytometry","volume":"11","author":"Liu","year":"2022","journal-title":"J. Extracell. Vesicles"},{"issue":"1","key":"2026093001075885400_cit20","doi-asserted-by":"publisher","DOI":"10.1038\/srep37246","article-title":"Digital Detection of Exosomes by Interferometric Imaging","volume":"6","author":"Daaboul","year":"2016","journal-title":"Sci. Rep."},{"key":"2026093001075885400_cit21","doi-asserted-by":"publisher","DOI":"10.3791\/62988","article-title":"Single-Particle Interferometric Reflectance Imaging Characterization of Individual Extracellular Vesicles and Population Dynamics","volume":"179","author":"Deng","year":"2022","journal-title":"JoVE"},{"issue":"1","key":"2026093001075885400_cit22","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11486-1","article-title":"Profiling surface proteins on individual exosomes using a proximity barcoding assay","volume":"10","author":"Wu","year":"2019","journal-title":"Nat. Commun."},{"issue":"11","key":"2026093001075885400_cit23","doi-asserted-by":"publisher","first-page":"4315","DOI":"10.1021\/acs.nanolett.2c00274","article-title":"Ultrasensitive Single Extracellular Vesicle Detection Using High Throughput Droplet Digital Enzyme-Linked Immunosorbent Assay","volume":"22","author":"Yang","year":"2022","journal-title":"Nano Lett."},{"issue":"7","key":"2026093001075885400_cit24","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12346","article-title":"Single Extracellular VEsicle Nanoscopy","volume":"12","author":"Saftics","year":"2023","journal-title":"J. Extracell. Vesicles"},{"issue":"15","key":"2026093001075885400_cit25","doi-asserted-by":"publisher","first-page":"7487","DOI":"10.1021\/cr500002z","article-title":"Porous Anodic Aluminum Oxide: Anodization and Templated Synthesis of Functional Nanostructures","volume":"114","author":"Lee","year":"2014","journal-title":"Chem. Rev."},{"issue":"2","key":"2026093001075885400_cit26","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1016\/j.memsci.2008.09.003","article-title":"Functionalized anodic aluminum oxide (AAO) membranes for affinity protein separation","volume":"325","author":"Shi","year":"2008","journal-title":"J. Membr. Sci."},{"issue":"8","key":"2026093001075885400_cit27","doi-asserted-by":"publisher","first-page":"8104","DOI":"10.1021\/nn502181k","article-title":"Functionalized Anodic Aluminum Oxide Membrane\u2212Electrode System for Enzyme Immobilization","volume":"8","author":"Chen","year":"2014","journal-title":"ACS Nano"},{"issue":"23","key":"2026093001075885400_cit28","doi-asserted-by":"publisher","first-page":"9913","DOI":"10.1021\/la047959a","article-title":"\u201cDirect\u201d Detection and Separation of DNA Using Nanoporous Alumina Filters","volume":"20","author":"Vlassiouk","year":"2004","journal-title":"Langmuir"},{"key":"2026093001075885400_cit29","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijleo.2019.06.003","article-title":"Label-free detecting oligonucleotide hybridization melting temperature in real-time with a reflectometric interference spectroscopy\u2212based nanosensor system","volume":"192","author":"Lu","year":"2019","journal-title":"Optik"},{"issue":"14","key":"2026093001075885400_cit30","doi-asserted-by":"publisher","first-page":"17074","DOI":"10.1021\/acsanm.4c03267","article-title":"Immobilization of Liposomes into Porous Anodized Aluminum Oxide for On-Demand Release by Ultrasonic Irradiation: Implications for Drug Delivery","volume":"7","author":"Okada","year":"2024","journal-title":"ACS Appl. Nano Mater."},{"key":"2026093001075885400_cit31","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.trac.2012.11.007","article-title":"Nanoporous anodic aluminum oxide for chemical sensing and biosensors","volume":"44","author":"Santos","year":"2013","journal-title":"TrAC, Trends Anal. Chem."},{"issue":"5","key":"2026093001075885400_cit32","doi-asserted-by":"publisher","first-page":"796","DOI":"10.1007\/s11095-010-0073-2","article-title":"Critical Evaluation of Nanoparticle Tracking Analysis (NTA) by NanoSight for the Measurement of Nanoparticles and Protein Aggregates","volume":"27","author":"Filipe","year":"2010","journal-title":"Pharm. Res."},{"key":"2026093001075885400_cit33","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9781139644181","volume-title":"Principles of Optics: Electromagnetic Theory of Propagation, Interference and Diffraction of Light","author":"Born","year":"1999"},{"issue":"6","key":"2026093001075885400_cit34","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1093\/mictod\/qaad088","article-title":"Igniting New Confocal Imaging Potential \u2212 Nikon AX R Series with NSPARC","volume":"31","author":"Delattre","year":"2023","journal-title":"Microsc. Today"},{"issue":"3","key":"2026093001075885400_cit35","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1080\/09537104.2016.1265926","article-title":"Bulk immunoassays for analysis of extracellular vesicles","volume":"28","author":"Coumans","year":"2017","journal-title":"Platelets"},{"issue":"7","key":"2026093001075885400_cit36","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202503002","article-title":"Advancing Cancer Diagnostics: Technologies for Quantifying the Particle Concentration of Extracellular Vesicles in Complex Biological Samples","volume":"15","author":"Str\u00f6hle","year":"2026","journal-title":"Adv. Healthcare Mater."},{"issue":"23","key":"2026093001075885400_cit37","doi-asserted-by":"publisher","first-page":"12968","DOI":"10.1021\/acs.analchem.7b03919","article-title":"Direct Exosome Quantification via Bivalent-Cholesterol-Labeled DNA Anchor for Signal Amplification","volume":"89","author":"He","year":"2017","journal-title":"Anal. Chem."},{"issue":"5","key":"2026093001075885400_cit38","doi-asserted-by":"publisher","first-page":"490","DOI":"10.1038\/nbt.2886","article-title":"Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor","volume":"32","author":"Im","year":"2014","journal-title":"Nat. Biotechnol."},{"issue":"8","key":"2026093001075885400_cit39","doi-asserted-by":"publisher","DOI":"10.1002\/jex2.53","article-title":"Comparing digital detection platforms in high sensitivity immune-phenotyping of extracellular vesicles","volume":"1","author":"Frigerio","year":"2022","journal-title":"J. Extracell. Biol."},{"issue":"1","key":"2026093001075885400_cit40","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-021-00987-1","article-title":"Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers","volume":"19","author":"Mizenko","year":"2021","journal-title":"J. Nanobiotechnol."},{"issue":"8","key":"2026093001075885400_cit41","doi-asserted-by":"publisher","first-page":"E968","DOI":"10.1073\/pnas.1521230113","article-title":"Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes","volume":"113","author":"Kowal","year":"2016","journal-title":"Proc. Natl. Acad. Sci."},{"issue":"1","key":"2026093001075885400_cit42","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-57617-9","article-title":"Uptake of small extracellular vesicles by recipient cells is facilitated by paracrine adhesion signaling","volume":"16","author":"Hirosawa","year":"2025","journal-title":"Nat. Commun."},{"issue":"1","key":"2026093001075885400_cit43","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-48095-3","article-title":"Extracellular vesicles isolated from porcine seminal plasma exhibit different tetraspanin expression profiles","volume":"9","author":"Barranco","year":"2019","journal-title":"Sci. Rep."},{"issue":"1","key":"2026093001075885400_cit44","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v5.29975","article-title":"A novel multiplex bead-based platform highlights the diversity of extracellular vesicles","volume":"5","author":"Koliha","year":"2016","journal-title":"J. Extracell. Vesicles"},{"issue":"7","key":"2026093001075885400_cit45","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1038\/s41556-024-01432-9","article-title":"CD63 sorts cholesterol into endosomes for storage and distribution via exosomes","volume":"26","author":"Palmulli","year":"2024","journal-title":"Nat. Cell Biol."},{"issue":"5","key":"2026093001075885400_cit46","doi-asserted-by":"publisher","first-page":"e02251","DOI":"10.1128\/jvi.02251-16","article-title":"CD63 Regulates Epstein-Barr Virus LMP1 Exosomal Packaging, Enhancement of Vesicle Production, and Noncanonical NF-\u03baB Signaling","volume":"91","author":"Hurwitz","year":"2017","journal-title":"J. Virol."},{"issue":"4","key":"2026093001075885400_cit47","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1016\/j.devcel.2011.08.019","article-title":"The Tetraspanin CD63 Regulates ESCRT-Independent and -Dependent Endosomal Sorting during Melanogenesis","volume":"21","author":"van Niel","year":"2011","journal-title":"Dev. Cell"},{"issue":"19","key":"2026093001075885400_cit48","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adi9156","article-title":"Endocytosis blocks the vesicular secretion of exosome marker proteins","volume":"10","author":"Ai","year":"2024","journal-title":"Sci. Adv."},{"issue":"3","key":"2026093001075885400_cit49","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2025.108255","article-title":"Tetraspanin CD9 alters cellular trafficking and endocytosis of tetraspanin CD63, affecting CD63 packaging into small extracellular vesicles","volume":"301","author":"Duke","year":"2025","journal-title":"J. Biol. Chem."},{"issue":"1","key":"2026093001075885400_cit50","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24384-2","article-title":"Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9","volume":"12","author":"Mathieu","year":"2021","journal-title":"Nat. Commun."},{"issue":"1","key":"2026093001075885400_cit51","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750","article-title":"Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"Th\u00e9ry","year":"2018","journal-title":"J. Extracell. Vesicles"},{"issue":"3","key":"2026093001075885400_cit52","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12047","article-title":"Single-vesicle imaging and co-localization analysis for tetraspanin profiling of individual extracellular vesicles","volume":"10","author":"Han","year":"2021","journal-title":"J. Extracell. Vesicles"},{"issue":"1","key":"2026093001075885400_cit53","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36932-z","article-title":"Multiplexed analysis of EV reveals specific biomarker composition with diagnostic impact","volume":"14","author":"Spitzberg","year":"2023","journal-title":"Nat. Commun."},{"issue":"27","key":"2026093001075885400_cit54","doi-asserted-by":"publisher","first-page":"22877","DOI":"10.1021\/acsami.8b05793","article-title":"Analysis of Extracellular Vesicles Using Coffee Ring","volume":"10","author":"Jeong","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"10","key":"2026093001075885400_cit55","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102394","article-title":"A shared, stochastic pathway mediates exosome protein budding along plasma and endosome membranes","volume":"298","author":"Fordjour","year":"2022","journal-title":"J. Biol. Chem."},{"key":"2026093001075885400_cit56","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.addr.2020.03.002","article-title":"An emerging focus on lipids in extracellular vesicles","volume":"159","author":"Skotland","year":"2020","journal-title":"Adv. Drug Delivery Rev."},{"issue":"2","key":"2026093001075885400_cit57","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202100785","article-title":"Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications","volume":"6","author":"Shimoda","year":"2022","journal-title":"Small Methods"},{"issue":"1","key":"2026093001075885400_cit58","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2019.100782","article-title":"Detection of Extracellular Vesicle RNA Using Molecular Beacons","volume":"23","author":"de Oliveira","year":"2020","journal-title":"iScience"},{"issue":"2","key":"2026093001075885400_cit59","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1021\/acssensors.2c02498","article-title":"Self-Assembly and Disassembly of Membrane Curvature-Sensing Peptide-Based Deep-Red Fluorescent Probe for Highly Sensitive Sensing of Exosomes","volume":"8","author":"Ohira","year":"2023","journal-title":"ACS Sens."},{"issue":"63","key":"2026093001075885400_cit60","doi-asserted-by":"publisher","first-page":"38323","DOI":"10.1039\/D0RA07763A","article-title":"Amphipathic helical peptide-based fluorogenic probes for a marker-free analysis of exosomes based on membrane-curvature sensing","volume":"10","author":"Sato","year":"2020","journal-title":"RSC Adv."},{"issue":"1","key":"2026093001075885400_cit61","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-08534-w","article-title":"Amphipathic helical peptide-Nile red probes for fluorescence probing of the lipid packing defects and their surrounding membranes on exosomes","volume":"15","author":"Sato","year":"2025","journal-title":"Sci. Rep."},{"issue":"48","key":"2026093001075885400_cit62","doi-asserted-by":"publisher","first-page":"17467","DOI":"10.1021\/acs.analchem.3c01501","article-title":"DNA-Encoded and Spatial Proximity Replaced Glycoprotein Analysis Reveals Glycosylation Heterogeneity of Extracellular Vesicles","volume":"95","author":"Li","year":"2023","journal-title":"Anal. Chem."},{"key":"2026093001075885400_cit63","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2025.343623","article-title":"Bioorthogonal click chemistry and aptamer-targeting enables highly selective fluorescence labeling of exosomal glycosylated EpCAM for super resolved imaging","volume":"1339","author":"Li","year":"2025","journal-title":"Anal. Chim. Acta"},{"issue":"41","key":"2026093001075885400_cit64","doi-asserted-by":"publisher","first-page":"15276","DOI":"10.1021\/acs.analchem.3c02594","article-title":"Nanoarray Enabled Size-Dependent Isolation and Proteomics Profiling of Small Extracellular Vesicle Subpopulations toward Accurate Cancer Diagnosis and Prognosis","volume":"95","author":"Wang","year":"2023","journal-title":"Anal. Chem."},{"key":"2026093001075885400_cit65","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2024.342576","article-title":"Efficient EVs separation and detection by an alumina-nanochannel-array-membrane integrated microfluidic chip and an antibody barcode biochip","volume":"1304","author":"Qiu","year":"2024","journal-title":"Anal. Chim. Acta"},{"issue":"2","key":"2026093001075885400_cit66","doi-asserted-by":"publisher","first-page":"1360","DOI":"10.1021\/acsnano.6b06131","article-title":"Exodisc for Rapid, Size-Selective, and Efficient Isolation and Analysis of Nanoscale Extracellular Vesicles from Biological Samples","volume":"11","author":"Woo","year":"2017","journal-title":"ACS Nano"},{"issue":"10","key":"2026093001075885400_cit67","doi-asserted-by":"publisher","first-page":"5480","DOI":"10.1021\/acs.analchem.4c04852","article-title":"Viral Membrane-Targeting Amphipathic Helical Peptide-Based Fluorogenic Probes for the Analysis of Infectious Titers of Enveloped Viruses","volume":"97","author":"Sato","year":"2025","journal-title":"Anal. Chem."},{"issue":"8","key":"2026093001075885400_cit68","doi-asserted-by":"publisher","first-page":"2005","DOI":"10.1039\/C3MB70109C","article-title":"Multivalency amplifies the selection and affinity of bradykinin-derived peptides for lipid nanovesicles","volume":"9","author":"Saludes","year":"2013","journal-title":"Mol. BioSyst."},{"issue":"4280","key":"2026093001075885400_cit69","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1126\/science.402030","article-title":"Membrane Asymmetry","volume":"195","author":"Rothman","year":"1977","journal-title":"Science"},{"issue":"1","key":"2026093001075885400_cit70","doi-asserted-by":"publisher","DOI":"10.1038\/srep33935","article-title":"A novel affinity-based method for the isolation of highly purified extracellular vesicles","volume":"6","author":"Nakai","year":"2016","journal-title":"Sci. Rep."}],"container-title":["ACS Applied Materials &amp; Interfaces"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsami.6c12881","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/18\/38\/52080\/68399706\/am-2026-12881g.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/18\/38\/52080\/68399706\/am-2026-12881g.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:13:43Z","timestamp":1790756023000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aamick\/article\/18\/38\/52080\/5422119\/Porous-Membrane-Adsorption-for-Capturing-Small"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,7]]},"references-count":70,"journal-issue":{"issue":"38","published-online":{"date-parts":[[2026,9,7]]},"published-print":{"date-parts":[[2026,9,30]]}},"URL":"https:\/\/doi.org\/10.1021\/acsami.6c12881","relation":{"has-preprint":[{"id-type":"doi","id":"10.26434\/chemrxiv-2025-dkbfr","asserted-by":"object"}]},"ISSN":["1944-8244","1944-8252"],"issn-type":[{"value":"1944-8244","type":"print"},{"value":"1944-8252","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,7]]}}}