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However, in-depth study linking enantioselective protein adsorption on chiral nanomaterials to antitumor efficacy is still scarce. In this study, chiral helical polyaniline (PANI) with left-handedness (M-PANI) and right-handedness (P-PANI) are constructed. Thermodynamic study and molecular dynamics simulations reveal the higher binding affinity of P-PANI toward glucose oxidase (GOx) than M-PANI. As a result, a greater quantity of GOx is adsorbed onto P-PANI, leading to the chirality-dependent amount of GOx in M\/P-PANI@GOx. In addition, P-PANI achieves stronger adsorption efficiency of D-glucose compared with M-PANI. Benefiting from these dual enantioselective effects, P-PANI@GOx displays superior catalytic efficiency for D-glucose oxidation. Furthermore, the M\/P-PANI@GOx is found to exhibit pH-responsive photothermal performance. Upon D-glucose oxidation, the higher activity of P-PANI@GOx causes a lower pH. Consequently, it induces a relatively higher local temperature under near-infrared (NIR) laser irradiation, which further triggers a more pronounced enhancement of D-glucose oxidation activity of P-PANI@GOx. Such cascaded chiral effects ultimately endow P-PANI@GOx with enhanced tumor cytotoxic efficacy and immunogenic cell death (ICD)-inducing capability. These findings highlight the crucial influence of chirality-mediated protein adsorption on in vitro antitumor efficacy, shedding light on the rational design of chiral nanomaterials for high-performance biological applications.<\/jats:p>","DOI":"10.1021\/acsami.6c14084","type":"journal-article","created":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:02:28Z","timestamp":1790373748000},"page":"53251-53263","source":"Crossref","is-referenced-by-count":0,"title":["Enantioselective Glucose Oxidase Adsorption on Helical Polyaniline for In Vitro Antitumor Effect"],"prefix":"10.1021","volume":"18","author":[{"given":"Qianyun","family":"Ye","sequence":"first","affiliation":[{"name":"Yangzhou University , , ,","place":["Yangzhou, China, 225002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sailu","family":"Wang","sequence":"additional","affiliation":[{"name":"Yangzhou University , , ,","place":["Yangzhou, China, 225002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8297-0972","authenticated-orcid":true,"given":"Shixin","family":"Li","sequence":"additional","affiliation":[{"name":"Yangzhou University , , ,","place":["Yangzhou, China, 225002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0050-7509","authenticated-orcid":true,"given":"Jie","family":"Han","sequence":"additional","affiliation":[{"name":"Yangzhou University , , ,","place":["Yangzhou, China, 225002"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5655-131X","authenticated-orcid":true,"given":"Xiaohuan","family":"Sun","sequence":"additional","affiliation":[{"name":"Yangzhou University , , ,","place":["Yangzhou, China, 225002"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,25]]},"reference":[{"issue":"34","key":"2026100711084318200_cit1","doi-asserted-by":"publisher","first-page":"4569","DOI":"10.1039\/d4cc00637b","article-title":"Phenylglycine Amphiphile-Metal Ion Chiral Supramolecular Nanozymes for Enantioselective Catalysis","volume":"60","author":"Li","year":"2024","journal-title":"Chem. 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