{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:30:22Z","timestamp":1790753422783,"version":"4.1.0"},"reference-count":64,"publisher":"American Chemical Society (ACS)","issue":"41","license":[{"start":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T00:00:00Z","timestamp":1505865600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T00:00:00Z","timestamp":1505865600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T00:00:00Z","timestamp":1505865600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100007052","name":"Memorial Sloan-Kettering Cancer Center","doi-asserted-by":"publisher","award":["U54CA199081"],"award-info":[{"award-number":["U54CA199081"]}],"id":[{"id":"10.13039\/100007052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000854","name":"Human Frontier Science Program","doi-asserted-by":"publisher","award":["LT000419\/2015"],"award-info":[{"award-number":["LT000419\/2015"]}],"id":[{"id":"10.13039\/501100000854","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000146","name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems","doi-asserted-by":"publisher","award":["CBET-1149452"],"award-info":[{"award-number":["CBET-1149452"]}],"id":[{"id":"10.13039\/100000146","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000146","name":"Division of Chemical, Bioengineering, Environmental, and Transport Systems","doi-asserted-by":"publisher","award":["CBET-1263701"],"award-info":[{"award-number":["CBET-1263701"]}],"id":[{"id":"10.13039\/100000146","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,10,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Emerging technologies use cell plasma membrane vesicles or \u201cblebs\u201d as an intermediate to form molecularly complete, planar cell surface mimetics that are compatible with a variety of characterization tools and microscopy methods. This approach enables direct incorporation of membrane proteins into supported lipid bilayers without using detergents and reconstitution and preserves native lipids and membrane species. Such a system can be advantageous as in vitro models of in vivo cell surfaces for study of the roles of membrane proteins as drug targets in drug delivery, host\u2013pathogen interactions, tissue engineering, and many other bioanalytical and sensing applications. However, the impact of methods used to induce cell blebbing (vesiculation) on protein and membrane properties is still unknown. This study focuses on characterization of cell blebs created under various bleb-inducing conditions and the result on protein properties (orientation, mobility, activity, etc.) and lipid scrambling in this platform. The orientation of proteins in the cell blebs and planar bilayers is revealed using a protease cleavage assay. Lipid scrambling in both cell blebs and planar bilayers is indicated through an annexin V binding assay. To quantify protein confinement, immobility, etc., incorporation of GPI-linked yellow fluorescent protein (GPI-YFP) was used in conjunction with single-molecule tracking (SMT) microscopy. Finally, to investigate the impact of the bleb induction method on protein activity and expression level, cell blebs expressing human aminopeptidase N (hAPN) were analyzed by an enzyme activity assay and immunoblotting. This work enriches our understanding of cell plasma membrane bleb bilayers as a biomimetic platform, reveals conditions under which specific properties are met, and represents one of the few ways to make molecularly complete supported bilayers directly from cell plasma membranes.<\/jats:p>","DOI":"10.1021\/acsami.7b07500","type":"journal-article","created":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T16:15:54Z","timestamp":1505924154000},"page":"35526-35538","source":"Crossref","is-referenced-by-count":42,"title":["Supported Planar Mammalian\nMembranes as Models of in Vivo Cell Surface Architectures"],"prefix":"10.1021","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9398-6969","authenticated-orcid":true,"given":"Han-Yuan","family":"Liu","sequence":"first","affiliation":[{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Grant","sequence":"additional","affiliation":[{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung-Lun","family":"Hsu","sequence":"additional","affiliation":[{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9006-5411","authenticated-orcid":true,"given":"Raya","family":"Sorkin","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Amsterdam , , ,","place":["Amsterdam, The Netherlands, 1081 HV"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filip","family":"Bos\u030ckovic\u0301","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Amsterdam , , ,","place":["Amsterdam, The Netherlands, 1081 HV"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gijs","family":"Wuite","sequence":"additional","affiliation":[{"name":"Vrije Universiteit Amsterdam , , ,","place":["Amsterdam, The Netherlands, 1081 HV"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7773-0835","authenticated-orcid":true,"given":"Susan","family":"Daniel","sequence":"additional","affiliation":[{"name":"Cornell University , , , ,","place":["Ithaca, New York, United States, 14853"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2017,9,20]]},"reference":[{"issue":"1","key":"2026081800121848500_cit1","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1038\/35036052","article-title":"Lipid Rafts and Signal Transduction","volume":"1","author":"Simons","year":"2000","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"12","key":"2026081800121848500_cit2","doi-asserted-by":"publisher","first-page":"6548","DOI":"10.1073\/pnas.111536798","article-title":"Synaptic\nPattern Formation During Cellular Recognition","volume":"98","author":"Qi","year":"2001","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"3","key":"2026081800121848500_cit3","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1038\/nrm1838","article-title":"Cell-Signalling Dynamics in Time\nand Space","volume":"7","author":"Kholodenko","year":"2006","journal-title":"Nat. Rev. Mol. Cell Biol."},{"issue":"12","key":"2026081800121848500_cit4","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1038\/nrn2257","article-title":"Regulation of Ion\nTransport Proteins by Membrane Phosphoinositides","volume":"8","author":"Gamper","year":"2007","journal-title":"Nat. Rev. Neurosci."},{"issue":"5961","key":"2026081800121848500_cit5","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1126\/science.1174621","article-title":"Lipid Rafts as a Membrane-Organizing\nPrinciple","volume":"327","author":"Lingwood","year":"2010","journal-title":"Science"},{"issue":"1","key":"2026081800121848500_cit6","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.bbamem.2004.05.012","article-title":"How Lipids Affect the Activities\nof Integral Membrane Proteins","volume":"1666","author":"Lee","year":"2004","journal-title":"Biochim. Biophys.\nActa, Biomembr."},{"issue":"1","key":"2026081800121848500_cit7","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.bbamem.2004.04.011","article-title":"Membrane Proteins,\nLipids and Detergents: Not Just a Soap Opera","volume":"1666","author":"Seddon","year":"2004","journal-title":"Biochim. Biophys. Acta, Biomembr."},{"issue":"1","key":"2026081800121848500_cit8","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/0304-4157(75)90016-7","article-title":"Solubilization of Membranes\nby Detergents","volume":"415","author":"Helenius","year":"1975","journal-title":"Biochim. Biophys. Acta, Rev.\nBiomembr."},{"issue":"8","key":"2026081800121848500_cit9","doi-asserted-by":"publisher","first-page":"3497","DOI":"10.1021\/la052687c","article-title":"Formation of Solid-Supported\nLipid Bilayers: An Integrated View","volume":"22","author":"Richter","year":"2006","journal-title":"Langmuir"},{"issue":"34","key":"2026081800121848500_cit10","doi-asserted-by":"publisher","first-page":"10363","DOI":"10.1021\/la501534f","article-title":"Solvent-Assisted\nLipid Bilayer Formation on Silicon Dioxide and Gold","volume":"30","author":"Tabaei","year":"2014","journal-title":"Langmuir"},{"issue":"1","key":"2026081800121848500_cit11","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/S0006-3495(85)83882-0","article-title":"Supported\nPhospholipid Bilayers","volume":"47","author":"Tamm","year":"1985","journal-title":"Biophys. J."},{"issue":"6058","key":"2026081800121848500_cit12","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1038\/320179a0","article-title":"T-Cell-Mediated\nAssociation of Peptide Antigen and Major Histocompatibility Complex\nProtein Detected by Energy Transfer in an Evanescent Wave-Field","volume":"320","author":"Watts","year":"1986","journal-title":"Nature"},{"issue":"11","key":"2026081800121848500_cit13","doi-asserted-by":"publisher","first-page":"2409","DOI":"10.1002\/cphc.200600464","article-title":"Combined Afm and Two-Focus Sfcs Study of Raft-Exhibiting\nModel Membranes","volume":"7","author":"Chiantia","year":"2006","journal-title":"ChemPhysChem"},{"issue":"6","key":"2026081800121848500_cit14","doi-asserted-by":"publisher","first-page":"1096","DOI":"10.1038\/nprot.2010.65","article-title":"Quartz Crystal Microbalance with\nDissipation Monitoring of Supported Lipid Bilayers on Various Substrates","volume":"5","author":"Cho","year":"2010","journal-title":"Nat. Protoc."},{"issue":"5","key":"2026081800121848500_cit15","doi-asserted-by":"publisher","first-page":"1361","DOI":"10.1021\/la00029a033","article-title":"Protein Binding to Supported Lipid Membranes: Investigation of the\nCholera Toxin-Ganglioside Interaction by Simultaneous Impedance Spectroscopy\nand Surface Plasmon Resonance","volume":"9","author":"Terrettaz","year":"1993","journal-title":"Langmuir"},{"issue":"12","key":"2026081800121848500_cit16","doi-asserted-by":"publisher","first-page":"2963","DOI":"10.1021\/acs.langmuir.5b03415","article-title":"Membrane Protein Mobility and Orientation\nPreserved in Supported Bilayers Created Directly from Cell Plasma\nMembrane Blebs","volume":"32","author":"Richards","year":"2016","journal-title":"Langmuir"},{"issue":"32","key":"2026081800121848500_cit17","doi-asserted-by":"publisher","first-page":"7895","DOI":"10.1016\/j.biomaterials.2013.06.034","article-title":"Single Particle Assay of Coronavirus\nMembrane Fusion with Proteinaceous Receptor-Embedded Supported Bilayers","volume":"34","author":"Costello","year":"2013","journal-title":"Biomaterials"},{"issue":"21","key":"2026081800121848500_cit18","doi-asserted-by":"publisher","first-page":"6409","DOI":"10.1021\/la400861u","article-title":"Membrane Fusion-Competent Virus-Like\nProteoliposomes and Proteinaceous Supported Bilayers Made Directly\nfrom Cell Plasma Membranes","volume":"29","author":"Costello","year":"2013","journal-title":"Langmuir"},{"issue":"2","key":"2026081800121848500_cit19","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1007\/BF00277781","article-title":"A Comparison of the Crosslinking\nAbilities of Glutaraldehyde, Formaldehyde and A-Hydroxyadipaldehyde\nwith Bovine Serum Albumin and Casein","volume":"17","author":"Hopwood","year":"1969","journal-title":"Histochem.\nCell Biol."},{"issue":"8","key":"2026081800121848500_cit20","doi-asserted-by":"publisher","first-page":"2673","DOI":"10.1021\/ja01188a018","article-title":"The Reaction of Formaldehyde with Proteins. V. Cross-Linking\nbetween Amino and Primary Amide or Guanidyl Groups","volume":"70","author":"Fraenkel-Conrat","year":"1948","journal-title":"J. Am. Chem. Soc."},{"issue":"6","key":"2026081800121848500_cit21","doi-asserted-by":"publisher","first-page":"1042","DOI":"10.1038\/nprot.2012.059","article-title":"Elucidating Membrane\nStructure and Protein Behavior Using Giant Plasma Membrane Vesicles","volume":"7","author":"Sezgin","year":"2012","journal-title":"Nat. Protoc."},{"issue":"51","key":"2026081800121848500_cit22","doi-asserted-by":"publisher","first-page":"22050","DOI":"10.1073\/pnas.1016184107","article-title":"Palmitoylation Regulates Raft Affinity\nfor the Majority of Integral Raft Proteins","volume":"107","author":"Levental","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"10","key":"2026081800121848500_cit23","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1042\/CBI20100147","article-title":"Secretion of the Heat Shock Proteins Hsp70 and Hsc70\nby Baby Hamster Kidney (Bhk-21) Cells","volume":"34","author":"Evdokimovskaya","year":"2010","journal-title":"Cell\nBiol. Int."},{"issue":"27","key":"2026081800121848500_cit24","doi-asserted-by":"publisher","first-page":"6991","DOI":"10.1021\/bi8006324","article-title":"The Glycosylphosphatidylinositol Anchor: A Complex\nMembrane-Anchoring Structure for Proteins\u2020","volume":"47","author":"Paulick","year":"2008","journal-title":"Biochemistry"},{"issue":"2","key":"2026081800121848500_cit25","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/S0304-4157(97)00005-1","article-title":"Mammalian\nGpi Proteins: Sorting, Membrane Residence and Functions","volume":"1331","author":"Nosjean","year":"1997","journal-title":"Biochim. Biophys. Acta, Rev. Biomembr."},{"issue":"1","key":"2026081800121848500_cit26","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1080\/09687680701551855","article-title":"Aminopeptidase N (Apn)\/Cd13-Dependent Cxcr4 Downregulation\nIs Associated with Diminished Cell Migration, Proliferation and Invasion","volume":"25","author":"Wulfaenger","year":"2008","journal-title":"Mol. Membr. Biol."},{"issue":"2","key":"2026081800121848500_cit27","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1182\/blood.V75.2.462.462","article-title":"Metalloprotease\nActivity of Cd13\/Aminopeptidase N on the Surface of Human Myeloid\nCells","volume":"75","author":"Ashmun","year":"1990","journal-title":"Blood"},{"issue":"6377","key":"2026081800121848500_cit28","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1038\/357420a0","article-title":"Human Aminopeptidase\nN Is a Receptor for Human Coronavirus 229e","volume":"357","author":"Yeager","year":"1992","journal-title":"Nature"},{"issue":"11","key":"2026081800121848500_cit29","doi-asserted-by":"publisher","first-page":"1393","DOI":"10.1038\/nbt1026","article-title":"Production of Recombinant Protein\nTherapeutics in Cultivated Mammalian Cells","volume":"22","author":"Wurm","year":"2004","journal-title":"Nat. Biotechnol."},{"issue":"2","key":"2026081800121848500_cit30","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1099\/0022-1317-79-2-347","article-title":"Highly Attenuated Modified Vaccinia\nVirus Ankara Replicates in Baby Hamster Kidney Cells, a Potential\nHost for Virus Propagation, but Not in Various Human Transformed and\nPrimary Cells","volume":"79","author":"Drexler","year":"1998","journal-title":"J. Gen. Virol."},{"issue":"5","key":"2026081800121848500_cit31","doi-asserted-by":"publisher","first-page":"1158","DOI":"10.1016\/j.biotechadv.2011.08.022","article-title":"Mammalian Cell Protein Expression\nfor Biopharmaceutical Production","volume":"30","author":"Zhu","year":"2012","journal-title":"Biotechnol.\nAdv."},{"issue":"1","key":"2026081800121848500_cit32","doi-asserted-by":"publisher","first-page":"013705","DOI":"10.1063\/1.2432410","article-title":"Wsxm: A Software for Scanning Probe Microscopy and a Tool for Nanotechnology","volume":"78","author":"Horcas","year":"2007","journal-title":"Rev. Sci. Instrum."},{"issue":"1","key":"2026081800121848500_cit33","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/0022-1759(95)00072-I","article-title":"A Novel Assay\nfor Apoptosis Flow Cytometric Detection of Phosphatidylserine Expression\non Early Apoptotic Cells Using Fluorescein Labelled Annexin V","volume":"184","author":"Vermes","year":"1995","journal-title":"J. Immunol. Methods"},{"key":"2026081800121848500_cit34","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1007\/978-1-59745-513-8_14","article-title":"Single-Molecule Tracking","volume":"398","author":"Vrljic","year":"2007","journal-title":"Methods\nMol. Biol."},{"issue":"5","key":"2026081800121848500_cit35","doi-asserted-by":"publisher","first-page":"2638","DOI":"10.1016\/S0006-3495(99)77097-9","article-title":"Free Brownian Motion of Individual\nLipid Molecules in Biomembranes","volume":"77","author":"Sonnleitner","year":"1999","journal-title":"Biophys. J."},{"key":"2026081800121848500_cit36","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1007\/978-1-62703-275-9_11","article-title":"A Guide to Tracking Single Transmembrane\nProteins in Supported Lipid Bilayers","volume":"974","author":"Poudel","year":"2013","journal-title":"Methods\nMol. Biol."},{"issue":"1","key":"2026081800121848500_cit37","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/S0167-2789(01)00234-2","article-title":"Strongly and Weakly Self-Similar\nDiffusion","volume":"154","author":"Ferrari","year":"2001","journal-title":"Phys. D"},{"issue":"7","key":"2026081800121848500_cit38","doi-asserted-by":"publisher","first-page":"1794","DOI":"10.1016\/j.bpj.2011.09.007","article-title":"Interactive,\nComputer-Assisted Tracking of Speckle Trajectories in Fluorescence\nMicroscopy: Application to Actin Polymerization and Membrane Fusion","volume":"101","author":"Smith","year":"2011","journal-title":"Biophys. J."},{"issue":"2","key":"2026081800121848500_cit39","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.jsb.2005.06.002","article-title":"Feature Point\nTracking and Trajectory Analysis for Video Imaging in Cell Biology","volume":"151","author":"Sbalzarini","year":"2005","journal-title":"J. Struct. Biol."},{"issue":"6","key":"2026081800121848500_cit40","doi-asserted-by":"publisher","first-page":"3331","DOI":"10.1016\/S0006-3495(99)77486-2","article-title":"Anomalous Diffusion of Major Histocompatibility\nComplex Class I Molecules on Hela Cells Determined by Single Particle\nTracking","volume":"76","author":"Smith","year":"1999","journal-title":"Biophys. J."},{"issue":"5","key":"2026081800121848500_cit41","doi-asserted-by":"publisher","first-page":"2021","DOI":"10.1016\/S0006-3495(93)81253-0","article-title":"Confined Lateral\nDiffusion of Membrane Receptors as Studied by Single Particle Tracking\n(Nanovid Microscopy). Effects of Calcium-Induced Differentiation in\nCultured Epithelial Cells","volume":"65","author":"Kusumi","year":"1993","journal-title":"Biophys. J."},{"issue":"8","key":"2026081800121848500_cit42","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nri2567","article-title":"Membrane Vesicles as Conveyors of\nImmune Responses","volume":"9","author":"The\u0301ry","year":"2009","journal-title":"Nat. Rev. Immunol."},{"issue":"R1","key":"2026081800121848500_cit43","doi-asserted-by":"publisher","first-page":"R125","DOI":"10.1093\/hmg\/dds317","article-title":"Exosomes and Microvesicles: Extracellular Vesicles\nfor Genetic Information Transfer and Gene Therapy","volume":"21","author":"Lee","year":"2012","journal-title":"Hum. Mol. Genet."},{"issue":"16","key":"2026081800121848500_cit44","doi-asserted-by":"publisher","first-page":"2667","DOI":"10.1007\/s00018-011-0689-3","article-title":"Membrane\nVesicles, Current State-of-the-Art: Emerging Role of Extracellular\nVesicles","volume":"68","author":"Gyo\u0308rgy","year":"2011","journal-title":"Cell. Mol. Life Sci."},{"issue":"4","key":"2026081800121848500_cit45","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1016\/j.bbamem.2009.12.019","article-title":"Nanomechanics of Lipid Bilayers by Force Spectroscopy with Afm: A\nPerspective","volume":"1798","author":"Garcia-Manyes","year":"2010","journal-title":"Biochim. Biophys. Acta, Biomembr."},{"issue":"4","key":"2026081800121848500_cit46","doi-asserted-by":"publisher","first-page":"1308","DOI":"10.1021\/la103927a","article-title":"Atomic Force Microscopy Force Mapping in the Study\nof Supported Lipid Bilayers","volume":"27","author":"Li","year":"2011","journal-title":"Langmuir"},{"issue":"2","key":"2026081800121848500_cit47","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1016\/j.bpj.2010.04.044","article-title":"Cholesterol-Dependent Nanomechanical\nStability of Phase-Segregated Multicomponent Lipid Bilayers","volume":"99","author":"Sullan","year":"2010","journal-title":"Biophys. J."},{"issue":"8","key":"2026081800121848500_cit48","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1038\/nmat3081","article-title":"Cell-Membrane Mechanics: Vesicles\nin and Tubes Out","volume":"10","author":"Dogterom","year":"2011","journal-title":"Nat. Mater."},{"issue":"5","key":"2026081800121848500_cit49","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1038\/nbt819","article-title":"A Method for the Comprehensive Proteomic Analysis of\nMembrane Proteins","volume":"21","author":"Wu","year":"2003","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"2026081800121848500_cit50","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/1477-5956-4-2","article-title":"Protein Cleavage Strategies\nfor an Improved Analysis of the Membrane Proteome","volume":"4","author":"Fischer","year":"2006","journal-title":"Proteome Sci."},{"key":"2026081800121848500_cit51","doi-asserted-by":"publisher","DOI":"10.1038\/srep32715","article-title":"A Molecularly Complete Planar\nBacterial Outer Membrane Platform","volume":"6","author":"Hsia","year":"2016","journal-title":"Sci. Rep."},{"issue":"13","key":"2026081800121848500_cit52","doi-asserted-by":"publisher","first-page":"4335","DOI":"10.1021\/la400119e","article-title":"Mechanisms of Vesicle Spreading on Surfaces: Coarse-Grained\nSimulations","volume":"29","author":"Fuhrmans","year":"2013","journal-title":"Langmuir"},{"issue":"12","key":"2026081800121848500_cit53","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1111\/tra.12008","article-title":"Simplified Equation to Extract Diffusion Coefficients\nfrom Confocal Frap Data","volume":"13","author":"Kang","year":"2012","journal-title":"Traffic"},{"issue":"1","key":"2026081800121848500_cit54","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.nano.2005.10.013","article-title":"Dynamics of Gpi-Anchored Proteins\non the Surface of Living Cells","volume":"2","author":"Nohe","year":"2006","journal-title":"Nanomedicine"},{"issue":"5","key":"2026081800121848500_cit55","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1083\/jcb.200312170","article-title":"Dynamics of Putative Raft-Associated\nProteins at the Cell Surface","volume":"165","author":"Kenworthy","year":"2004","journal-title":"J. Cell Biol."},{"issue":"4","key":"2026081800121848500_cit56","doi-asserted-by":"publisher","first-page":"e34923","DOI":"10.1371\/journal.pone.0034923","article-title":"Mechanisms\nUnderlying the Confined Diffusion of Cholera Toxin B-Subunit in Intact\nCell Membranes","volume":"7","author":"Day","year":"2012","journal-title":"PLoS One"},{"issue":"5","key":"2026081800121848500_cit57","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1038\/nchembio737","article-title":"Neuronal Synapse Interaction Reconstituted between\nLive Cells and Supported Lipid Bilayers","volume":"1","author":"Pautot","year":"2005","journal-title":"Nat.\nChem. Biol."},{"issue":"2","key":"2026081800121848500_cit58","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1006\/bbrc.1998.8663","article-title":"Essential\nRole of Phosphatidylserine Externalization in Apoptosing Cell Phagocytosis\nby Macrophages","volume":"246","author":"Shiratsuchi","year":"1998","journal-title":"Biochem. Biophys. Res. Commun."},{"issue":"12","key":"2026081800121848500_cit59","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.1016\/S0006-2952(98)00225-1","article-title":"A Necessary Role for Cell Shrinkage in Apoptosis","volume":"56","author":"Bortner","year":"1998","journal-title":"Biochem. Pharmacol."},{"key":"2026081800121848500_cit60","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-01542-5","article-title":"The Rational Design of Therapeutic\nPeptides for Aminopeptidase N Using a Substrate-Based Approach","volume":"7","author":"Joshi","year":"2017","journal-title":"Sci. Rep."},{"issue":"4","key":"2026081800121848500_cit61","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1083\/jcb.201211138","article-title":"Extracellular\nVesicles: Exosomes, Microvesicles, and Friends","volume":"200","author":"Raposo","year":"2013","journal-title":"J. Cell Biol."},{"issue":"6","key":"2026081800121848500_cit62","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1016\/j.tcb.2015.01.004","article-title":"Ectosomes and Exosomes: Shedding the Confusion between\nExtracellular Vesicles","volume":"25","author":"Cocucci","year":"2015","journal-title":"Trends Cell Biol."},{"issue":"11","key":"2026081800121848500_cit63","doi-asserted-by":"crossref","first-page":"3791","DOI":"10.1182\/blood.V94.11.3791","article-title":"Activated\nPlatelets Release Two Types of Membrane Vesicles: Microvesicles by\nSurface Shedding and Exosomes Derived from Exocytosis of Multivesicular\nBodies and Alpha-Granules","volume":"94","author":"Heijnen","year":"1999","journal-title":"Blood"},{"issue":"4266","key":"2026081800121848500_cit64","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1126\/science.982044","article-title":"Plasma Membrane Vesiculation: A New\nTechnique for Isolation of Plasma Membranes","volume":"194","author":"Scott","year":"1976","journal-title":"Science"}],"container-title":["ACS Applied Materials &amp; Interfaces"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsami.7b07500","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/9\/41\/35526\/7109496\/am7b07500.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/9\/41\/35526\/7109496\/am7b07500.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:41:54Z","timestamp":1787042514000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aamick\/article\/9\/41\/35526\/705002\/Supported-Planar-Mammalian-Membranes-as-Models-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,20]]},"references-count":64,"journal-issue":{"issue":"41","published-online":{"date-parts":[[2017,9,20]]},"published-print":{"date-parts":[[2017,10,18]]}},"URL":"https:\/\/doi.org\/10.1021\/acsami.7b07500","relation":{},"ISSN":["1944-8244","1944-8252"],"issn-type":[{"value":"1944-8244","type":"print"},{"value":"1944-8252","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,20]]}}}