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We present here the design and fabrication of a bifunctional Janus GBR membrane based on a shear flow-driven layer by a layer self-assembly approach. The Janus GBR membrane comprises a calcium phosphate\u2013collagen\/polyethylene glycol (CaP@COL\/PEG) layer and a chitosan\/poly\u00ad(acrylic acid) (CHI\/PAA) layer on different sides of a collagen membrane to form a sandwich structure. The membrane exhibits good mechanical stability and tailored biodegradability. It is found that the CaP@COL\/PEG layer and CHI\/PAA layer contribute to the osteogenic differentiation and antibacterial function, respectively. In comparison with the control group, the Janus GBR membrane displays a 2.52-time and 1.84-time enhancement in respective volume and density of newly generated bone. The greatly improved bone repair ability of the Janus GBR membrane is further confirmed through histological analysis, and it has great potential for practical applications in bone tissue engineering.<\/jats:p>","DOI":"10.1021\/acsbiomaterials.4c00420","type":"journal-article","created":{"date-parts":[[2024,5,10]],"date-time":"2024-05-10T14:40:26Z","timestamp":1715352026000},"page":"3984-3993","source":"Crossref","is-referenced-by-count":14,"title":["Shear Flow-Assembled Janus Membrane with Bifunctional\nOsteogenic and Antibacterial Effects for Guided Bone Regeneration"],"prefix":"10.1021","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7670-5619","authenticated-orcid":true,"given":"Zequn","family":"Ma","sequence":"first","affiliation":[{"name":"Suzhou University of Science and Technology , , 99 Xuefu Road , , ,","place":["Suzhou, Jiangsu, China, 215009"]},{"name":"China University of Geosciences , , ,","place":["Beijing, China, 100083"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiantong","family":"Hu","sequence":"additional","affiliation":[{"name":"Beijing Engineering Research Center of Orthopedics Implants , , ,","place":["Beijing, China, 100048"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2766-896X","authenticated-orcid":true,"given":"Xiangming","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong University of Petrochemical Technology , , ,","place":["Maoming, China, 525000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3050-2979","authenticated-orcid":true,"given":"Qi","family":"An","sequence":"additional","affiliation":[{"name":"China University of Geosciences , , ,","place":["Beijing, China, 100083"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Suzhou University of Science and Technology , , 99 Xuefu Road , , ,","place":["Suzhou, Jiangsu, China, 215009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2603-7181","authenticated-orcid":true,"given":"Chunxian","family":"Guo","sequence":"additional","affiliation":[{"name":"Suzhou University of Science and Technology , , 99 Xuefu Road , , ,","place":["Suzhou, Jiangsu, China, 215009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yantao","family":"Zhao","sequence":"additional","affiliation":[{"name":"Beijing Engineering Research Center of Orthopedics Implants , , ,","place":["Beijing, China, 100048"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1407-4129","authenticated-orcid":true,"given":"Yihe","family":"Zhang","sequence":"additional","affiliation":[{"name":"China University of Geosciences , , ,","place":["Beijing, China, 100083"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2024,5,10]]},"reference":[{"issue":"7","key":"2026081411092732900_cit1","doi-asserted-by":"publisher","first-page":"1143","DOI":"10.1002\/adma.201403354","article-title":"Natural-based nanocomposites for\nbone tissue engineering and regenerative medicine: A review","volume":"27","author":"Pina","year":"2015","journal-title":"Adv. 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Eng. J."},{"issue":"11","key":"2026081411092732900_cit26","doi-asserted-by":"publisher","first-page":"1480","DOI":"10.1021\/acs.accounts.2c00052","article-title":"Self-assembly\nof nanowires: From dynamic monitoring to precision control","volume":"55","author":"He","year":"2022","journal-title":"Acc. Chem. 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Mater."},{"key":"2026081411092732900_cit30","doi-asserted-by":"publisher","first-page":"134820","DOI":"10.1016\/j.cej.2022.134820","article-title":"Orchestrated tumor\napoptosis (Cu2+) and bone tissue calcification\n(Ca2+) by hierarchical copper\/calcium-ensembled bioactive\nsilica for osteosarcoma therapy","volume":"435","author":"Han","year":"2022","journal-title":"Chem. Eng.\nJ."},{"key":"2026081411092732900_cit31","doi-asserted-by":"publisher","first-page":"105901","DOI":"10.1016\/j.nanoen.2021.105901","article-title":"Remotely\ncontrolled self-powering electrical stimulators for osteogenic differentiation\nusing bone inspired bioactive piezoelectric whitlockite nanoparticles","volume":"85","author":"Kaliannagounder","year":"2021","journal-title":"Nano Energy"},{"issue":"44","key":"2026081411092732900_cit32","doi-asserted-by":"publisher","first-page":"1909874","DOI":"10.1002\/adfm.201909874","article-title":"Functional\nbiomaterials for bone regeneration: A lesson in complex biology","volume":"30","author":"Armiento","year":"2020","journal-title":"Adv. Funct. 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Mater."},{"key":"2026081411092732900_cit36","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1016\/j.bioactmat.2021.05.037","article-title":"Cerium oxide\nnanoparticles loaded nanofibrous membranes promote bone regeneration\nfor periodontal tissue engineering","volume":"7","author":"Ren","year":"2022","journal-title":"Bioact.\nMater."}],"container-title":["ACS Biomaterials Science &amp; Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsbiomaterials.4c00420","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/abseba\/article-pdf\/10\/6\/3984\/18532304\/ab4c00420.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/abseba\/article-pdf\/10\/6\/3984\/18532304\/ab4c00420.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T15:28:01Z","timestamp":1786721281000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/abseba\/article\/10\/6\/3984\/95134\/Shear-Flow-Assembled-Janus-Membrane-with"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,10]]},"references-count":36,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,5,10]]},"published-print":{"date-parts":[[2024,6,10]]}},"URL":"https:\/\/doi.org\/10.1021\/acsbiomaterials.4c00420","relation":{},"ISSN":["2373-9878"],"issn-type":[{"value":"2373-9878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,10]]}}}