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Regenerative strategies combining mesenchymal stem cells (MSCs) with biomaterials offer great potential for NP repair by replenishing cells and restoring extracellular matrix (ECM). However, key translational challenges remain, including limited stem cell differentiation, poor cell survival in the harsh degenerative niche, and insufficient biomaterial support. While matrix viscoelasticity has been shown to influence adipose-derived stem cell (ASC) discogenic differentiation, its interplay with cell-adhesive ligands for IVD regeneration remains unclear. Moreover, most current hydrogels fail to replicate the ultrafast stress relaxation properties of native non-degenerative human NP tissue. Here, we developed viscoelastic ECM peptide-functionalized hydrogels (VEPH), specifically designed to mimic healthy human NP biomechanics and promote ASC differentiation for NP regeneration. We biochemically conjugated NP ECM-derived adhesive peptides (IKVAV, hA5G26, CHAD) through maleimide-thiol click chemistry, achieving hydrogels with significantly faster stress relaxation (\u223c25 s) compared to conventional viscoelastic alginate hydrogels (&amp;gt;100 s). Our results demonstrated that VEPH supported &amp;gt;95% ASC viability and robust metabolic activity over 21 days in 3D culture. Notably, the IKVAV-functionalized hydrogel significantly enhanced ASC cell-matrix interactions, upregulated NP marker expression (KRT18, HIF-1\u03b1, ITGA3, and CD24), and promoted type-II collagen secretion, indicating an NP-committed cell fate. Our findings highlight the synergistic roles of matrix viscoelasticity and NP-specific biochemical cues in directing ASC discogenic differentiation and advancing novel biomaterial design for IVD regeneration.<\/jats:p>","DOI":"10.1021\/acsbiomaterials.5c02074","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:02:21Z","timestamp":1788523341000},"page":"5478-5497","source":"Crossref","is-referenced-by-count":0,"title":["Biomimetic Hydrogels with Nucleus Pulposus-like Viscoelasticity and ECM Peptides for Discogenic Differentiation of Stem Cells"],"prefix":"10.1021","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7874-2301","authenticated-orcid":true,"given":"Yin","family":"Liu","sequence":"first","affiliation":[{"name":"McGill University , , 3775 Rue University , , ,","place":["Montr\u00e9al, QC, Canada, H3A 2B4"]},{"name":"McGill University , , 817 Sherbrooke Street West , , ,","place":["Montr\u00e9al, QC, Canada, H3A 0C3"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixun","family":"Cheng","sequence":"additional","affiliation":[{"name":"McGill University , , 817 Sherbrooke Street West , , ,","place":["Montr\u00e9al, QC, Canada, H3A 0C3"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Johnston","sequence":"additional","affiliation":[{"name":"McGill University , , 817 Sherbrooke Street West , , ,","place":["Montr\u00e9al, QC, Canada, H3A 0C3"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7402-0587","authenticated-orcid":true,"given":"Shuaibing","family":"Jiang","sequence":"additional","affiliation":[{"name":"McGill University , , 817 Sherbrooke Street West , , ,","place":["Montr\u00e9al, QC, Canada, H3A 0C3"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hosni","family":"Cherif","sequence":"additional","affiliation":[{"name":"McGill University , , 1650 Cedar Avenue , , ,","place":["Montr\u00e9al, QC, Canada, H3G 1A4"]},{"name":"McGill University , , 1001 Bd D\u00e9carie , , ,","place":["Montr\u00e9al, QC, Canada, H4A 3J1"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7313-1162","authenticated-orcid":true,"given":"David","family":"Juncker","sequence":"additional","affiliation":[{"name":"McGill University , , 3775 Rue University , , ,","place":["Montr\u00e9al, QC, Canada, H3A 2B4"]},{"name":"McGill University & Genome Quebec Innovation Center , 740 Avenue Dr. Penfield , , ,","place":["Montr\u00e9al, QC, Canada, H4A 0G1"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole Y. K.","family":"Li-Jessen","sequence":"additional","affiliation":[{"name":"McGill University , , 3775 Rue University , , ,","place":["Montr\u00e9al, QC, Canada, H3A 2B4"]},{"name":"McGill University , , 1001 Bd D\u00e9carie , , ,","place":["Montr\u00e9al, QC, Canada, H4A 3J1"]},{"name":"McGill University , , 2001TypNodi146 McGill College Avenue , , ,","place":["Montr\u00e9al, QC, Canada, H3A 1G1"]},{"name":"McGill University Health Centre , , 1001 Bd D\u00e9carie , , ,","place":["Montr\u00e9al, QC, Canada, H4A 3J1"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1288-2149","authenticated-orcid":true,"given":"Lisbet","family":"Haglund","sequence":"additional","affiliation":[{"name":"McGill University , , 1650 Cedar Avenue , , ,","place":["Montr\u00e9al, QC, Canada, H3G 1A4"]},{"name":"McGill University , , 1001 Bd D\u00e9carie , , ,","place":["Montr\u00e9al, QC, Canada, H4A 3J1"]},{"name":"Shriners Hospital for Children , 1003 Bd D\u00e9carie , , ,","place":["Montr\u00e9al, QC, Canada, H4A 0A9"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1128-6804","authenticated-orcid":true,"given":"Jianyu","family":"Li","sequence":"additional","affiliation":[{"name":"McGill University , , 3775 Rue University , , ,","place":["Montr\u00e9al, QC, Canada, H3A 2B4"]},{"name":"McGill University , , 817 Sherbrooke Street West , , ,","place":["Montr\u00e9al, QC, Canada, H3A 0C3"]},{"name":"McGill University , , 1650 Cedar Avenue , , ,","place":["Montr\u00e9al, QC, Canada, H3G 1A4"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"2026101202063718300_cit1","doi-asserted-by":"publisher","first-page":"e316","DOI":"10.1016\/S2665-9913(23)00098-X","article-title":"Global, regional, and national burden of low back pain, 1990-2020, its attributable risk factors, and projections to 2050: a systematic analysis of the Global Burden of Disease Study 2021","volume":"5","author":"Ferreira","year":"2023","journal-title":"Lancet Rheumatol."},{"key":"2026101202063718300_cit2","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1038\/nrrheum.2015.13","article-title":"Stem cell therapy for intervertebral disc regeneration: obstacles and solutions","volume":"11","author":"Sakai","year":"2015","journal-title":"Nat. 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