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This study developed calcium-binding poly(butyl methacrylate-co-methacryloyloxyethyl phosphate) (PBMP)-coated poly(lactide-co-glycolide) (PLGA) microparticles to enhance calcium-rich surface anchoring and enable sustained BMP-2 release. Microparticles were characterized for morphology, zeta potential, encapsulation efficiency, release kinetics, and hydroxyapatite (HA)-binding affinity, and were further evaluated for in vivo efficacy. PBMP coating markedly increased the HA-binding affinity of PLGA microparticles. Encapsulation efficiency was over 55%, and BMP-2 was released in a sustained manner over 30 days, with the cumulative release gradually approaching a plateau during the later incubation period. When applied to a rat calvarial defect model, the PBMP-coated microparticle (BMP-2-PLGA\/PBMP) group showed the greatest bone volume fraction, bone surface area, and trabecular number, along with the lowest trabecular separation compared to the free BMP-2 and control groups. Histological analysis confirmed denser and more mature bone matrix formation in the BMP-2-PLGA\/PBMP group. These findings demonstrate that PBMP-coated PLGA microparticles provide HA-targeted, sustained BMP-2 delivery, resulting in improved bone regeneration and microarchitecture. This delivery platform represents a promising strategy for more effective bone defect repair.<\/jats:p>","DOI":"10.1021\/acsbiomaterials.6c00581","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:02:07Z","timestamp":1790154127000},"page":"5465-5477","source":"Crossref","is-referenced-by-count":0,"title":["Calcium-Specific-Binding PLGA Microspheres with Embedded BMP-2 for Bone Regeneration"],"prefix":"10.1021","volume":"12","author":[{"given":"Minjae","family":"Kim","sequence":"first","affiliation":[{"name":"Seoul National University , , 1 Gwanak-ro , Gwanak-gu, ,","place":["Seoul, Republic of Korea, 08826"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0235-1261","authenticated-orcid":true,"given":"Jun Ho","family":"Park","sequence":"additional","affiliation":[{"name":"SMG-SNU Boramae Medical Center , , 20 Boramae-ro 5-gil , Dongjak-gu, ,","place":["Seoul, Republic of Korea, 07061"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min-Ha","family":"Choi","sequence":"additional","affiliation":[{"name":"SMG-SNU Boramae Medical Center , , 20 Boramae-ro 5-gil , Dongjak-gu, ,","place":["Seoul, Republic of Korea, 07061"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2498-1301","authenticated-orcid":true,"given":"Yan","family":"Lee","sequence":"additional","affiliation":[{"name":"Seoul National University , , 1 Gwanak-ro , Gwanak-gu, ,","place":["Seoul, Republic of Korea, 08826"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji-Ung","family":"Park","sequence":"additional","affiliation":[{"name":"SMG-SNU Boramae Medical Center , , 20 Boramae-ro 5-gil , Dongjak-gu, ,","place":["Seoul, Republic of Korea, 07061"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,23]]},"reference":[{"issue":"9","key":"2026101202070750300_cit1","doi-asserted-by":"publisher","first-page":"563","DOI":"10.5435\/00124635-201109000-00007","article-title":"Management of aseptic tibial nonunion","volume":"19","author":"Hak","year":"2011","journal-title":"J. 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