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The persistence of M1 macrophages impairs the timely resolution of acute inflammation, thereby delaying tissue healing and compromising biomaterial performance. The decellularized amnion membrane (DAM) exhibits notable anti-inflammatory properties. However, its limited mechanical strength and poor degradation resistance restrict its clinical applications. This study employed genipin cross-linking to improve the mechanical strength and enzymatic degradation resistance of DAM, resulting in a genipin-cross-linked DAM scaffold (GED) with enhanced anti-inflammatory properties. GED demonstrated markedly enhanced mechanical strength, thermal stability, and degradation resistance, retaining its structural integrity even after 28 days of collagenase-mediated degradation in vitro. Moreover, GED exhibited the ability to scavenge excess reactive oxygen species (ROS) and suppress LPS-induced pro-inflammatory cytokine expression in macrophages, thereby effectively mitigating inflammation. This research provides new insights into the development of extracellular matrix-based materials and highlights GED as a promising multifunctional anti-inflammatory scaffold for tissue engineering applications.<\/jats:p>","DOI":"10.1021\/acsbiomaterials.6c01480","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:01:54Z","timestamp":1789473714000},"page":"5697-5708","source":"Crossref","is-referenced-by-count":0,"title":["Genipin-Cross-Linked Decellularized Amnion Membrane with Antioxidant and Anti-Inflammatory Properties"],"prefix":"10.1021","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0036-5503","authenticated-orcid":true,"given":"Tong","family":"Zhang","sequence":"first","affiliation":[{"name":"Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , , ,","place":["Beijing, P. R. China, 100190"]},{"name":"University of Chinese Academy of Sciences , , ,","place":["Beijing, P. R. 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