{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,9]],"date-time":"2026-11-09T13:24:34Z","timestamp":1794230674720,"version":"4.9.0"},"reference-count":40,"publisher":"American Chemical Society (ACS)","issue":"11","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100023581","name":"National Science Foundation Graduate Research Fellowship Program","doi-asserted-by":"publisher","award":["DGE2139757"],"award-info":[{"award-number":["DGE2139757"]}],"id":[{"id":"10.13039\/100023581","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY035853"],"award-info":[{"award-number":["R01EY035853"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R33HL154252"],"award-info":[{"award-number":["R33HL154252"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,11,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Smooth muscle cells (SMCs) are a critical cellular component of the tunica media of blood vessels in arteries and arterioles. Despite their importance, the development of in vitro arteriole models with diameters &amp;lt;200 \u00b5m remains challenging, largely due to difficulties in culturing SMCs at these length scales. In particular, uncontrolled SMC overproliferation can lead to microvessel vessel narrowing and occlusion. Here, we report a 3D microfluidic-based tissue-engineered model with human brain SMCs forming a microvessel approximately 150 \u03bcm in diameter within a collagen I hydrogel. Using this platform, we demonstrate that serum-free culture conditions are essential to prevent SMC overproliferation and maintain vessel patency. In addition, SMCs cultured in serum-free and serum-supplemented conditions exhibit distinct functional phenotypes, consistent with previous studies in 2D culture. These results provide a strategy for fabrication of tissue-engineered arteriole models.<\/jats:p>","DOI":"10.1021\/acsbiomaterials.6c01600","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T16:02:14Z","timestamp":1791907334000},"page":"6460-6470","source":"Crossref","is-referenced-by-count":0,"title":["Effect of Serum on Smooth Muscle Cell Phenotype in 3D Tissue-Engineered Microvessels"],"prefix":"10.1021","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1907-6008","authenticated-orcid":true,"given":"Lily","family":"Liang","sequence":"first","affiliation":[{"name":"Johns Hopkins University , , 3400 N. Charles St , , ,","place":["Baltimore, Maryland, United States, 21218"]},{"name":"Johns Hopkins University, Institute for Nanobiotechnology , 3400 N. Charles St , , ,","place":["Baltimore, Maryland, United States, 21218"]},{"name":"Kansas State University , , 1701D Platt St. , , ,","place":["Manhattan, Kansas, United States, 88506"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4700-3841","authenticated-orcid":true,"given":"Yunhan","family":"Liao","sequence":"additional","affiliation":[{"name":"Johns Hopkins University , , 3400 N. Charles St , , ,","place":["Baltimore, Maryland, United States, 21218"]},{"name":"Johns Hopkins University, Institute for Nanobiotechnology , 3400 N. Charles St , , ,","place":["Baltimore, Maryland, United States, 21218"]},{"name":"Kansas State University , , 1701D Platt St. , , ,","place":["Manhattan, Kansas, United States, 88506"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2942-1170","authenticated-orcid":true,"given":"Ninghao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Johns Hopkins University, Institute for Nanobiotechnology , 3400 N. Charles St , , ,","place":["Baltimore, Maryland, United States, 21218"]},{"name":"Kansas State University , , 1701D Platt St. , , ,","place":["Manhattan, Kansas, United States, 88506"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5417-0828","authenticated-orcid":true,"given":"Peter C.","family":"Searson","sequence":"additional","affiliation":[{"name":"Johns Hopkins University , , 3400 N. Charles St , , ,","place":["Baltimore, Maryland, United States, 21218"]},{"name":"Johns Hopkins University, Institute for Nanobiotechnology , 3400 N. Charles St , , ,","place":["Baltimore, Maryland, United States, 21218"]},{"name":"Kansas State University , , 1701D Platt St. , , ,","place":["Manhattan, Kansas, United States, 88506"]},{"name":"Johns Hopkins University , , 3400 N. Charles St , , ,","place":["Baltimore, Maryland, United States, 21218"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"2026110907072913900_cit1","first-page":"183","volume-title":"Int. Rev. 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(Torino)"},{"issue":"5","key":"2026110907072913900_cit37","doi-asserted-by":"publisher","first-page":"H1537","DOI":"10.1152\/ajpheart.00891.2009","article-title":"Structural inhomogeneity and fiber orientation in the inner arterial media","volume":"298","author":"Timmins","year":"2010","journal-title":"Am. J. Physiol.-Heart Circ. 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