{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:31:21Z","timestamp":1790667081216,"version":"4.1.0"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T00:00:00Z","timestamp":1587340800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci?n","doi-asserted-by":"publisher","award":["PCT-010000-2010-4"],"award-info":[{"award-number":["PCT-010000-2010-4"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom?a y Competitividad","doi-asserted-by":"publisher","award":["BIO2014-56752-R"],"award-info":[{"award-number":["BIO2014-56752-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom?a y Competitividad","doi-asserted-by":"publisher","award":["PIM2010EEI-00752"],"award-info":[{"award-number":["PIM2010EEI-00752"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011941","name":"Gobierno del Principado de Asturias","doi-asserted-by":"publisher","award":["FC-15-GRUPIN14-014"],"award-info":[{"award-number":["FC-15-GRUPIN14-014"]}],"id":[{"id":"10.13039\/100011941","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Largimycins are hybrid nonribosomal peptide-polyketides that constitute a new group of metabolites in the leinamycin family of natural products displaying unique structural features such as containing an oxazole instead of a thiazole ring or being oxime ester macrocycles, unprecedented in nature, rather than macrolactams. Their discovery in Streptomyces argillaceus and Streptomyces canus has relied on the activation of two homologous silent gene clusters by overexpressing a transcriptional activator and cultivating in specific media. The proposed biosynthesis of largimycins includes the key action of the oxidoreductase LrgO, responsible for the formation of the oxime group involved in macrocyclization, and two putative cryptic biosynthetic steps consisting of chlorination of l-Thr by the NRPS loading module and incorporation of an olefinic exomethylene group by LrgJ PKS. The discovery of largimycins uncovers novel biosynthetic avenues employed in nature to enrich the structural diversity of leinamycins and provides tools for combinatorial biosynthesis.<\/jats:p>","DOI":"10.1021\/acschembio.0c00160","type":"journal-article","created":{"date-parts":[[2020,4,20]],"date-time":"2020-04-20T14:13:22Z","timestamp":1587392002000},"page":"1541-1553","source":"Crossref","is-referenced-by-count":32,"title":["Discovery of Cryptic Largimycins in\n                    <i>Streptomyces<\/i>\n                    Reveals Novel Biosynthetic Avenues Enriching the Structural Diversity\nof the Leinamycin Family"],"prefix":"10.1021","volume":"15","author":[{"given":"Adriana","family":"Becerril","sequence":"first","affiliation":[{"name":"Universidad de Oviedo , , ,","place":["Oviedo, Spain"]},{"name":"Instituto de Investigacio\u0301n Sanitaria de Asturias (ISPA) , ,","place":["Oviedo, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4556-688X","authenticated-orcid":true,"given":"Ignacio","family":"Pe\u0301rez-Victoria","sequence":"additional","affiliation":[{"name":"Centro de Excelencia en Investigacio\u0301n de Medicamentos Innovadores en Andaluci\u0301a , , , ,","place":["Armilla, Granada, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhui","family":"Ye","sequence":"additional","affiliation":[{"name":"Universidad de Oviedo , , ,","place":["Oviedo, Spain"]},{"name":"Instituto de Investigacio\u0301n Sanitaria de Asturias (ISPA) , ,","place":["Oviedo, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo F.","family":"Bran\u0303a","sequence":"additional","affiliation":[{"name":"Universidad de Oviedo , , ,","place":["Oviedo, Spain"]},{"name":"Instituto de Investigacio\u0301n Sanitaria de Asturias (ISPA) , ,","place":["Oviedo, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesu\u0301s","family":"Marti\u0301n","sequence":"additional","affiliation":[{"name":"Centro de Excelencia en Investigacio\u0301n de Medicamentos Innovadores en Andaluci\u0301a , , , ,","place":["Armilla, Granada, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1607-5106","authenticated-orcid":true,"given":"Fernando","family":"Reyes","sequence":"additional","affiliation":[{"name":"Centro de Excelencia en Investigacio\u0301n de Medicamentos Innovadores en Andaluci\u0301a , , , ,","place":["Armilla, Granada, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 A.","family":"Salas","sequence":"additional","affiliation":[{"name":"Universidad de Oviedo , , ,","place":["Oviedo, Spain"]},{"name":"Instituto de Investigacio\u0301n Sanitaria de Asturias (ISPA) , ,","place":["Oviedo, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2729-841X","authenticated-orcid":true,"given":"Carmen","family":"Me\u0301ndez","sequence":"additional","affiliation":[{"name":"Universidad de Oviedo , , ,","place":["Oviedo, Spain"]},{"name":"Instituto de Investigacio\u0301n Sanitaria de Asturias (ISPA) , ,","place":["Oviedo, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,4,20]]},"reference":[{"key":"2026081714190155300_cit1","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1021\/acs.jnatprod.5b01055","article-title":"Natural products as sources of new drugs from 1981\nto 2014","volume":"79","author":"Newman","year":"2016","journal-title":"J. Nat. Prod."},{"key":"2026081714190155300_cit2","doi-asserted-by":"publisher","first-page":"1768","DOI":"10.7164\/antibiotics.42.1768","article-title":"Leinamycin, a new antitumor antibiotic from Streptomyces: producing organism, fermentation and isolation","volume":"42","author":"Kara","year":"1989","journal-title":"J. Antibiot."},{"key":"2026081714190155300_cit3","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1021\/tx900301r","article-title":"Characterization of\nDNA damage induced by a natural product antitumor antibiotic leinamycin\nin human cancer cells","volume":"23","author":"Viswesh","year":"2010","journal-title":"Chem. Res. Toxicol."},{"key":"2026081714190155300_cit4","doi-asserted-by":"publisher","first-page":"3149","DOI":"10.1073\/pnas.0537286100","article-title":"Type I polyketide\nsynthase requiring a discrete acyltransferase for polyketide biosynthesis","volume":"100","author":"Cheng","year":"2003","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026081714190155300_cit5","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1021\/ol102838y","article-title":"Characterization of\nthe lnmKLM genes unveiling key intermediates for\n\u03b2-alkylation in leinamycin biosynthesis","volume":"13","author":"Huang","year":"2011","journal-title":"Org. Lett."},{"key":"2026081714190155300_cit6","doi-asserted-by":"publisher","first-page":"10359","DOI":"10.1073\/pnas.1508437112","article-title":"C-S bond cleavage by a polyketide synthase domain","volume":"112","author":"Ma","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026081714190155300_cit7","doi-asserted-by":"publisher","first-page":"E11131","DOI":"10.1073\/pnas.1716245115","article-title":"Discovery\nof the leinamycin family of natural products by mining actinobacterial\ngenomes","volume":"114","author":"Pan","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026081714190155300_cit8","doi-asserted-by":"publisher","first-page":"1362","DOI":"10.1039\/b817069j","article-title":"Genomic\nbasis for natural product biosynthetic diversity in the actinomycetes","volume":"26","author":"Nett","year":"2009","journal-title":"Nat. Prod. Rep."},{"key":"2026081714190155300_cit9","first-page":"1","volume-title":"Strategies for the Design and Discovery of Novel Antibiotics Using\nGenetic Engineering and Genome Mining. In Antimicrobial Compounds:\nCurrent Strategies and New Alternatives","author":"Olano","year":"2014"},{"key":"2026081714190155300_cit10","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1038\/nrmicro3496","article-title":"Discovery of microbial natural products by activation\nof silent biosynthetic gene clusters","volume":"13","author":"Rutledge","year":"2015","journal-title":"Nat. Rev.\nMicrobiol."},{"key":"2026081714190155300_cit11","doi-asserted-by":"publisher","first-page":"828","DOI":"10.1111\/1751-7915.13398","article-title":"Novel bioactive natural products\nfrom bacteria via bioprospecting, genome mining and metabolic engineering","volume":"12","author":"Sekurova","year":"2019","journal-title":"Microb. Biotechnol."},{"key":"2026081714190155300_cit12","doi-asserted-by":"publisher","first-page":"194","DOI":"10.3389\/fmicb.2017.00194","article-title":"Identification\nby genome mining of a type I polyketide gene cluster from Streptomyces argillaceus involved in the biosynthesis of\npyridine and piperidine alkaloids argimycins P","volume":"8","author":"Ye","year":"2017","journal-title":"Front. Microbiol."},{"key":"2026081714190155300_cit13","doi-asserted-by":"publisher","first-page":"1326","DOI":"10.1055\/s-0035-1557876","article-title":"Expanding the chemical diversity\nof the antitumoral compound mithramycin by combinatorial biosynthesis\nand biocatalysis: the quest for mithralogs with improved therapeutic\nwindow","volume":"81","author":"Me\u0301ndez","year":"2015","journal-title":"Planta Med."},{"key":"2026081714190155300_cit14","doi-asserted-by":"publisher","first-page":"e0198145","DOI":"10.1371\/journal.pone.0198145","article-title":"Uncovering\nproduction of specialized metabolites by Streptomyces argillaceus: activation of cryptic biosynthesis gene clusters using nutritional\nand genetic approaches","volume":"13","author":"Becerril","year":"2018","journal-title":"PLoS One"},{"key":"2026081714190155300_cit15","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.chembiol.2003.12.014","article-title":"Leinamycin\nbiosynthesis revealing unprecedented architectural complexity for\na hybrid polyketide synthase and nonribosomal peptide synthetase","volume":"11","author":"Tang","year":"2004","journal-title":"Chem. Biol."},{"key":"2026081714190155300_cit16","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1002\/1439-7633(20020703)3:7&lt;619::AID-CBIC619&gt;3.0.CO;2-9","article-title":"Big effects from small changes: possible ways to explore nature\u2019s\nchemical diversity","volume":"3","author":"Bode","year":"2002","journal-title":"ChemBioChem"},{"key":"2026081714190155300_cit17","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1021\/jo00922a001","article-title":"Nuclear\nmagnetic resonance spectroscopy. Use of carbon-13 spectra to establish\nconfigurations of oximes","volume":"39","author":"Hawkes","year":"1974","journal-title":"J. Org. Chem."},{"key":"2026081714190155300_cit18","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1246\/bcsj.72.1093","article-title":"A practical synthesis of (1S,2R)-1-amino-2-indanol, a key component of an HIV protease\ninhibitor, indinavir","volume":"72","author":"Kajiro","year":"1999","journal-title":"Bull. Chem. Soc. Jpn."},{"key":"2026081714190155300_cit19","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1002\/mrc.2396","article-title":"The application\nof empirical methods of 13C NMR chemical shift prediction\nas a filter for determining possible relative stereochemistry","volume":"47","author":"Elyashberg","year":"2009","journal-title":"Magn. Reson. Chem."},{"key":"2026081714190155300_cit20","doi-asserted-by":"publisher","first-page":"8278","DOI":"10.1073\/pnas.1506761112","article-title":"Leinamycin E1 acting as an anticancer prodrug activated by reactive\noxygen species","volume":"112","author":"Huang","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S.\nA."},{"key":"2026081714190155300_cit21","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1002\/open.201500198","article-title":"Novel natural\noximes and oxime esters with a vibralactone backbone from the basidiomycete Boreostereum vibrans","volume":"5","author":"Chen","year":"2016","journal-title":"ChemistryOpen"},{"key":"2026081714190155300_cit22","doi-asserted-by":"publisher","first-page":"4009","DOI":"10.1128\/JB.177.14.4009-4020.1995","article-title":"Analysis\nof the syrB and syrC genes of Pseudomonas syringae pv. syringae indicates\nthat syringomycin is synthesized by a thiotemplate mechanism","volume":"177","author":"Zhang","year":"1995","journal-title":"J. Bacteriol."},{"key":"2026081714190155300_cit23","doi-asserted-by":"publisher","first-page":"5799","DOI":"10.1093\/nar\/gki885","article-title":"Specificity prediction of adenylation\ndomains in nonribosomal peptide synthetases (NRPS) using transductive\nsupport vector machines (TSVMs)","volume":"33","author":"Rausch","year":"2005","journal-title":"Nucleic Acids\nRes."},{"key":"2026081714190155300_cit24","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1016\/S1074-5521(99)80082-9","article-title":"The specificity-conferring code of adenylation domains\nin nonribosomal peptide synthetases","volume":"6","author":"Stachelhaus","year":"1999","journal-title":"Chem. Biol."},{"key":"2026081714190155300_cit25","doi-asserted-by":"publisher","first-page":"10111","DOI":"10.1073\/pnas.0504412102","article-title":"SyrB2 in\nsyringomycin E biosynthesis is a nonheme FeII alpha-ketoglutarate-\nand O2-dependent halogenase","volume":"102","author":"Vaillancourt","year":"2005","journal-title":"Proc. Natl. Acad.\nSci. U. S. A."},{"key":"2026081714190155300_cit26","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.chembiol.2006.11.005","article-title":"Characterization\nof SyrC, an aminoacyltransferase shuttling threonyl and chlorothreonyl\nresidues in the syringomycin biosynthetic assembly line","volume":"14","author":"Singh","year":"2007","journal-title":"Chem. Biol."},{"key":"2026081714190155300_cit27","doi-asserted-by":"publisher","first-page":"1191","DOI":"10.1038\/nature03797","article-title":"Cryptic chlorination by a non-haem\niron enzyme during cyclopropyl amino acid biosynthesis","volume":"436","author":"Vaillancourt","year":"2005","journal-title":"Nature"},{"key":"2026081714190155300_cit28","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1021\/bi011852u","article-title":"Catalytic mapping of the vibriobactin\nbiosynthetic enzyme VibF","volume":"41","author":"Marshall","year":"2002","journal-title":"Biochemistry"},{"key":"2026081714190155300_cit29","doi-asserted-by":"publisher","first-page":"9722","DOI":"10.1021\/bi034792w","article-title":"Oxidase\ndomains in epothilone and bleomycin biosynthesis: thiazoline to thiazole\noxidation during chain elongation","volume":"42","author":"Schneider","year":"2003","journal-title":"Biochemistry"},{"key":"2026081714190155300_cit30","doi-asserted-by":"publisher","first-page":"98","DOI":"10.3109\/10409238.2012.745476","article-title":"Structural\nanalysis of protein\u2013protein interactions in type I polyketide\nsynthases","volume":"48","author":"Xu","year":"2013","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"2026081714190155300_cit31","doi-asserted-by":"publisher","first-page":"4288","DOI":"10.1021\/acs.orglett.6b02033","article-title":"Characterization of the ketosynthase\nand acyl carrier protein domains at the LnmI nonribosomal peptide\nsynthetase-polyketide synthase interface for leinamycin biosynthesis","volume":"18","author":"Huang","year":"2016","journal-title":"Org. Lett."},{"key":"2026081714190155300_cit32","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1039\/C5NP00125K","article-title":"Biosynthesis of polyketides by trans-AT\npolyketide synthases","volume":"33","author":"Helfrich","year":"2016","journal-title":"Nat. Prod. Rep."},{"key":"2026081714190155300_cit33","doi-asserted-by":"publisher","first-page":"8977","DOI":"10.1073\/pnas.0603148103","article-title":"Convergence\nof isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus\nsubtilis","volume":"103","author":"Calderone","year":"2006","journal-title":"Proc. Natl. Acad. Sci. U.\nS. A."},{"key":"2026081714190155300_cit34","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1016\/S0022-2836(03)00232-8","article-title":"Computational approach for prediction\nof domain organization and substrate specificity of modular polyketide\nsynthases","volume":"328","author":"Yadav","year":"2003","journal-title":"J. Mol. Biol."},{"key":"2026081714190155300_cit35","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1016\/S1074-5521(96)90181-7","article-title":"A new enzyme superfamily- the phosphopantetheinyl\ntransferases","volume":"3","author":"Lambalot","year":"1996","journal-title":"Chem. Biol."},{"key":"2026081714190155300_cit36","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1016\/S1074-5521(01)00002-3","article-title":"Role of\ntype II thioesterases: evidence for removal of short acyl chains produced\nby aberrant decarboxylation of chain extender units","volume":"8","author":"Heathcote","year":"2001","journal-title":"Chem. Biol."},{"key":"2026081714190155300_cit37","doi-asserted-by":"publisher","first-page":"1022","DOI":"10.1016\/j.chembiol.2013.06.014","article-title":"Engineering the biosynthesis of the polyketide-nonribosomal\npeptide collismycin A for generation of analogs with neuroprotective\nactivity","volume":"20","author":"Garcia","year":"2013","journal-title":"Chem. Biol."},{"key":"2026081714190155300_cit38","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1038\/ja.2013.119","article-title":"Cytochrome P450-mediated\nhydroxylation is required for polyketide macrolactonization in stambomycin\nbiosynthesis","volume":"67","author":"Song","year":"2014","journal-title":"J. Antibiot."},{"key":"2026081714190155300_cit39","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1002\/cbic.200700017","article-title":"3-hydroxy-3-methylglutaryl-CoA-like\nsynthases direct the formation of methyl and ethyl side groups in\nthe biosynthesis of the antibiotic myxovirescin A","volume":"8","author":"Simunovic","year":"2007","journal-title":"ChemBioChem"},{"key":"2026081714190155300_cit40","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1039\/C7NP00034K","article-title":"Cytochromes P450 for\nnatural product biosynthesis in Streptomyces: sequence, structure,\nand function","volume":"34","author":"Rudolf","year":"2017","journal-title":"Nat. Prod. Rep."},{"key":"2026081714190155300_cit41","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1002\/cbic.201300704","article-title":"Insights into\nthe bioactivity of mensacarcin and epoxide formation by MsnO8","volume":"15","author":"Maier","year":"2014","journal-title":"ChemBioChem"},{"key":"2026081714190155300_cit42","doi-asserted-by":"publisher","first-page":"6802","DOI":"10.1021\/ja960892w","article-title":"Thiol-mediated DNA alkylation by\nthe novel antitumor antibiotic leinamycin","volume":"118","author":"Asai","year":"1996","journal-title":"J.\nAm. Chem. Soc."},{"key":"2026081714190155300_cit43","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1016\/j.redox.2018.08.017","article-title":"Redox regulation\nby reversible protein S-thiolation in Gram-positive bacteria","volume":"20","author":"Imber","year":"2019","journal-title":"Redox Biol."},{"key":"2026081714190155300_cit44","doi-asserted-by":"publisher","first-page":"1091","DOI":"10.1039\/b616489g","article-title":"Mycothiol: synthesis, biosynthesis\nand biological functions of the major low molecular weight thiol in\nactinomycetes","volume":"25","author":"Jothivasan","year":"2008","journal-title":"Nat. Prod. Rep."},{"key":"2026081714190155300_cit45","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/B978-0-08-095167-6.00710-2","article-title":"Hydrolysis and reverse hydrolysis:\nhalohydrin dehalogenases","volume-title":"Comprehensive\nChirality","author":"Schallmey","year":"2012"}],"container-title":["ACS Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acschembio.0c00160","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/15\/6\/1541\/5890258\/cb0c00160.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/15\/6\/1541\/5890258\/cb0c00160.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:36:42Z","timestamp":1786991802000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acbcct\/article\/15\/6\/1541\/588767\/Discovery-of-Cryptic-Largimycins-in-Streptomyces"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,20]]},"references-count":45,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,4,20]]},"published-print":{"date-parts":[[2020,6,19]]}},"URL":"https:\/\/doi.org\/10.1021\/acschembio.0c00160","relation":{},"ISSN":["1554-8929","1554-8937"],"issn-type":[{"value":"1554-8929","type":"print"},{"value":"1554-8937","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,20]]}}}