{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:31:46Z","timestamp":1786995106078,"version":"build-2736575974"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T00:00:00Z","timestamp":1589846400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T00:00:00Z","timestamp":1589846400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T00:00:00Z","timestamp":1589846400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["GM118185"],"award-info":[{"award-number":["GM118185"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005302","name":"University of Illinois at Urbana-Champaign","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005302","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Many protein ion channels harness membrane potential to move ions in opposition to their chemical gradient. Deficiencies of such proteins cause several human diseases, including cystic fibrosis, Bartter Syndrome, and proximal renal tubular acidosis. Using yeast as a eukaryotic model system, we asked whether, in the context of a protein ion channel deficiency in vivo, small molecule channels could similarly harness membrane potential to concentrate ions. Trk potassium transporters use membrane potential to move potassium from a relatively low concentration outside cells (\u223c15 mM) to one of &amp;gt;10\u00d7 higher inside (150\u2013500 mM); trk1\u0394trk2\u0394 are unable to concentrate potassium or grow in standard media. Here we show that potassium-permeable, but not potassium-selective, small-molecule ion channels formed by amphotericin B can harness membrane potential to concentrate potassium and thereby restore trk1\u0394trk2\u0394 growth. This finding expands the list of potential human channelopathies that might be addressed by a molecular prosthetics approach.<\/jats:p>","DOI":"10.1021\/acschembio.0c00180","type":"journal-article","created":{"date-parts":[[2020,5,19]],"date-time":"2020-05-19T17:27:51Z","timestamp":1589909271000},"page":"1575-1580","source":"Crossref","is-referenced-by-count":10,"title":["Small Molecule Channels Harness Membrane Potential\nto Concentrate Potassium in trk1\u0394trk2\u0394 Yeast"],"prefix":"10.1021","volume":"15","author":[{"given":"Jennifer","family":"Hou","sequence":"first","affiliation":[{"name":"University of Illinois at Urbana\u2212Champaign , , 600 South Mathews Ave. , , ,","place":["Urbana, Illinois, United States, 61801"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Page N.","family":"Daniels","sequence":"additional","affiliation":[{"name":"University of Illinois at Urbana\u2212Champaign , , 600 South Mathews Ave. , , ,","place":["Urbana, Illinois, United States, 61801"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7963-7140","authenticated-orcid":true,"given":"Martin D.","family":"Burke","sequence":"additional","affiliation":[{"name":"University of Illinois at Urbana\u2212Champaign , , 600 South Mathews Ave. , , ,","place":["Urbana, Illinois, United States, 61801"]},{"name":"University of Illinois at Urbana\u2212Champaign , , 600 South Mathews Ave. , , ,","place":["Urbana, Illinois, United\nStates, 61801"]},{"name":"Carle Illinois College of Medicine , 807 South Wright Street , , ,","place":["Champaign, Illinois, United\nStates, 61820"]},{"name":"University of Illinois at Urbana\u2212Champaign , , 1206 West Gregory Dr. , , ,","place":["Urbana, Illinois, United States, 61801"]},{"name":"University of Illinois at Urbana\u2212Champaign , , 405 North Mathews Ave. , , ,","place":["Urbana, Illinois, United States, 61801"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,5,19]]},"reference":[{"issue":"5753","key":"2026081714192837700_cit1","doi-asserted-by":"publisher","first-page":"1461","DOI":"10.1126\/science.1113666","article-title":"Principles\nof selective ion transport in channels and\npumps","volume":"310","author":"Gouaux","year":"2005","journal-title":"Science"},{"issue":"11","key":"2026081714192837700_cit2","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.1038\/ncb1104-1039","article-title":"Ion channels: function unravelled\nby dysfunction","volume":"6","author":"Jentsch","year":"2004","journal-title":"Nat. Cell Biol."},{"issue":"7083","key":"2026081714192837700_cit3","doi-asserted-by":"publisher","first-page":"440","DOI":"10.1038\/nature04707","article-title":"From molecule\nto malady","volume":"440","author":"Ashcroft","year":"2006","journal-title":"Nature"},{"key":"2026081714192837700_cit4","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1056\/NEJMra1300109","article-title":"Origins of Cystic Fibrosis Lung Disease","volume":"372","author":"Stoltz","year":"2015","journal-title":"N. Engl. J. 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Chem., Int.\nEd."},{"key":"2026081714192837700_cit8","doi-asserted-by":"publisher","first-page":"L1164","DOI":"10.1152\/ajplung.2001.281.5.L1164","article-title":"Partial correction of defective Cl\u2013 secretion in cystic fibrosis epithelial cells by an analog of squalamine","volume":"281","author":"Jiang","year":"2001","journal-title":"Am. J. Physiol. Lung Cell. Mol. Physiol."},{"key":"2026081714192837700_cit9","doi-asserted-by":"publisher","first-page":"4931","DOI":"10.1002\/anie.200351957","article-title":"Chloride\ntransport across vesicle\nand cell membranes by steroid-based receptors","volume":"42","author":"Koulov","year":"2003","journal-title":"Angew. Chem., Int. 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Chem."}],"container-title":["ACS Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acschembio.0c00180","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/15\/6\/1575\/5891113\/cb0c00180.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/15\/6\/1575\/5891113\/cb0c00180.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:43:39Z","timestamp":1786992219000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acbcct\/article\/15\/6\/1575\/588847\/Small-Molecule-Channels-Harness-Membrane-Potential"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,19]]},"references-count":33,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,5,19]]},"published-print":{"date-parts":[[2020,6,19]]}},"URL":"https:\/\/doi.org\/10.1021\/acschembio.0c00180","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.04.06.028365","asserted-by":"object"}]},"ISSN":["1554-8929","1554-8937"],"issn-type":[{"value":"1554-8929","type":"print"},{"value":"1554-8937","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,19]]}}}