{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:20:30Z","timestamp":1788297630373,"version":"build-2803163510"},"reference-count":30,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2020,5,13]],"date-time":"2020-05-13T00:00:00Z","timestamp":1589328000000},"content-version":"vor","delay-in-days":5,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_ccby_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["100993\/Z\/13\/Z"],"award-info":[{"award-number":["100993\/Z\/13\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Research Councils UK","doi-asserted-by":"publisher","award":["EP\/L015498\/1"],"award-info":[{"award-number":["EP\/L015498\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Malaria remains an endemic tropical disease, and the emergence of Plasmodium falciparum parasites resistant to current front-line medicines means that new therapeutic targets are required. The Plasmodium glideosome is a multiprotein complex thought to be essential for efficient host red blood cell invasion. At its core is a myosin motor, Myosin A (MyoA), which provides most of the force required for parasite invasion. Here, we report the design and development of improved peptide-based probes for the anchor point of MyoA, the P. falciparum MyoA tail interacting protein (PfMTIP). These probes combine low nanomolar binding affinity with significantly enhanced cell penetration and demonstrable competitive target engagement with native PfMTIP through a combination of Western blot and chemical proteomics. These results provide new insights into the potential druggability of the MTIP\/MyoA interaction and a basis for the future design of inhibitors.<\/jats:p>","DOI":"10.1021\/acschembio.0c00328","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T13:56:33Z","timestamp":1588946193000},"page":"1313-1320","source":"Crossref","is-referenced-by-count":11,"title":["Peptide Probes for\n                    <i>Plasmodium falciparum<\/i>\n                    MyoA Tail Interacting Protein (MTIP): Exploring the Druggability\nof the Malaria Parasite Motor Complex"],"prefix":"10.1021","volume":"15","author":[{"given":"Charlie N.","family":"Saunders","sequence":"first","affiliation":[{"name":"Molecular Sciences Research Hub , , White City Campus, Wood Lane , , ,","place":["London, United Kingdom, W12 0BZ"]},{"name":"Molecular Sciences Research Hub , , White City Campus, Wood Lane , , ,","place":["London, United Kingdom, W12 0BZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Cota","sequence":"additional","affiliation":[{"name":"Imperial College , , ,","place":["London, United Kingdom, SW7 2AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jake","family":"Baum","sequence":"additional","affiliation":[{"name":"Imperial College , , ,","place":["London, United Kingdom, SW7 2AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2213-5814","authenticated-orcid":true,"given":"Edward W.","family":"Tate","sequence":"additional","affiliation":[{"name":"Molecular Sciences Research Hub , , White City Campus, Wood Lane , , ,","place":["London, United Kingdom, W12 0BZ"]},{"name":"Molecular Sciences Research Hub , , White City Campus, Wood Lane , , ,","place":["London, United Kingdom, W12 0BZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,5,8]]},"reference":[{"key":"2026090117101391900_cit1","unstructured":"World Malaria Report\n2017.  WHO Reports; World Health Organzation, 2018; p 196."},{"issue":"45","key":"2026090117101391900_cit2","doi-asserted-by":"publisher","first-page":"E10548","DOI":"10.1073\/pnas.1811167115","article-title":"Structural\nand Mechanistic Insights into the Function of the Unconventional Class\nXIV Myosin MyoA from Toxoplasma Gondii","volume":"115","author":"Powell","year":"2018","journal-title":"Proc.\nNatl. Acad. Sci. U. S. A."},{"issue":"7549","key":"2026090117101391900_cit3","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1038\/nature14412","article-title":"A Molecular Mechanism of Artemisinin Resistance\nin Plasmodium Falciparum Malaria","volume":"520","author":"Mbengue","year":"2015","journal-title":"Nature"},{"issue":"9","key":"2026090117101391900_cit4","doi-asserted-by":"publisher","first-page":"953","DOI":"10.1016\/j.semcdb.2011.09.022","article-title":"The Mechanism of\nErythrocyte Invasion by the Malarial\nParasite, Plasmodium Falciparum","volume":"22","author":"Farrow","year":"2011","journal-title":"Semin. Cell\nDev. Biol."},{"issue":"44","key":"2026090117101391900_cit5","doi-asserted-by":"publisher","first-page":"36968","DOI":"10.1074\/jbc.M112.379842","article-title":"Regulation\nof the Plasmodium Motor Complex: Phosphorylation of Myosin a Tail-Interacting\nProtein (MTIP) Loosens Its Grip on MyoA","volume":"287","author":"Douse","year":"2012","journal-title":"J.\nBiol. Chem."},{"issue":"3","key":"2026090117101391900_cit6","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1039\/b922093c","article-title":"Interaction\nand Dynamics of the Plasmodium Falciparum MTIP-MyoA Complex, a Key\nComponent of the Invasion Motor in the Malaria Parasite","volume":"6","author":"Thomas","year":"2010","journal-title":"Mol. BioSyst."},{"issue":"8","key":"2026090117101391900_cit7","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1038\/nrmicro1465","article-title":"Regulation\nof Apicomplexan Actin-Based Motility","volume":"4","author":"Baum","year":"2006","journal-title":"Nat. Rev.\nMicrobiol."},{"issue":"3","key":"2026090117101391900_cit8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091819","article-title":"The Toxoplasma Acto-MyoA Motor Complex Is Important\nbut Not Essential for Gliding Motility and Host Cell Invasion","volume":"9","author":"Egarter","year":"2014","journal-title":"PLoS One"},{"issue":"1","key":"2026090117101391900_cit9","doi-asserted-by":"publisher","first-page":"3286","DOI":"10.1038\/s41467-019-11120-0","article-title":"Plasmodium Myosin A Drives Parasite Invasion by an\nAtypical Force Generating Mechanism","volume":"10","author":"Robert-Paganin","year":"2019","journal-title":"Nat. Commun."},{"issue":"13","key":"2026090117101391900_cit10","doi-asserted-by":"publisher","first-page":"4852","DOI":"10.1073\/pnas.0510907103","article-title":"Structure\nof the MTIP-MyoA Complex, a Key Component\nof the Malaria Parasite Invasion Motor","volume":"103","author":"Bosch","year":"2006","journal-title":"Proc.\nNatl. Acad. Sci. U. S. 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Parasitol."},{"issue":"5","key":"2026090117101391900_cit13","doi-asserted-by":"publisher","first-page":"933","DOI":"10.1016\/j.jmb.2005.11.027","article-title":"The MTIP-Myosin\nA Complex in Blood Stage Malaria Parasites","volume":"355","author":"Green","year":"2006","journal-title":"J. Mol. Biol."},{"issue":"43","key":"2026090117101391900_cit14","doi-asserted-by":"publisher","first-page":"17857","DOI":"10.1074\/jbc.M117.802769","article-title":"Compositional and Expression\nAnalyses of the Glideosome during the\nPlasmodium Life Cycle Reveal an Additional Myosin Light Chain Required\nfor Maximum Motility","volume":"292","author":"Green","year":"2017","journal-title":"J. Biol. Chem."},{"issue":"12","key":"2026090117101391900_cit15","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.010678","article-title":"The Malaria\nParasite Progressively Dismantles the Host Erythrocyte Cytoskeleton\nfor Efficient Egress","volume":"10","author":"Millholland","year":"2011","journal-title":"Mol. Cell. Proteomics"},{"issue":"1","key":"2026090117101391900_cit16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/cbdv.201200393","article-title":"Permeation\nthrough Phospholipid Bilayers, Skin-Cell Penetration, Plasma Stability,\nand CD Spectra of \u03b1- And \u03b2-Oligoproline Derivatives","volume":"10","author":"Kolesinska","year":"2013","journal-title":"Chem. Biodiversity"},{"issue":"1","key":"2026090117101391900_cit17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/cbdv.201000318","article-title":"On the Mechanism of\nEukaryotic Cell Penetration by\n\u03b1- and \u03b2-Oligoarginines--Targeting Infected Erythrocytes","volume":"8","author":"Kamena","year":"2011","journal-title":"Chem. Biodiversity"},{"issue":"15\u201316","key":"2026090117101391900_cit18","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1016\/j.drudis.2012.03.002","article-title":"Cell-Penetrating\nPeptides: Classes, Origin, and Current Landscape","volume":"17","author":"Milletti","year":"2012","journal-title":"Drug Discovery Today"},{"issue":"10","key":"2026090117101391900_cit19","doi-asserted-by":"publisher","first-page":"4689","DOI":"10.1128\/AAC.00427-13","article-title":"Improved Efficacy\nof Fosmidomycin against Plasmodium and Mycobacterium Species by Combination\nwith the Cell-Penetrating Peptide Octaarginine","volume":"57","author":"Sparr","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"issue":"10","key":"2026090117101391900_cit20","doi-asserted-by":"publisher","first-page":"2204","DOI":"10.1021\/cb500271c","article-title":"Crystal\nStructures of Stapled and\nHydrogen Bond Surrogate Peptides Targeting a Fully Buried Protein-Helix\nInteraction","volume":"9","author":"Douse","year":"2014","journal-title":"ACS Chem. Biol."},{"issue":"1","key":"2026090117101391900_cit21","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1039\/C4MD00131A","article-title":"Towards Understanding Cell Penetration\nby Stapled Peptides","volume":"6","author":"Chu","year":"2015","journal-title":"MedChemComm"},{"issue":"5","key":"2026090117101391900_cit22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129239","article-title":"The Stapled\nAKAP Disruptor Peptide\nSTAD-2 Displays Antimalarial Activity through a PKA-Independent Mechanism","volume":"10","author":"Flaherty","year":"2015","journal-title":"PLoS One"},{"issue":"2","key":"2026090117101391900_cit23","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/j.bbamem.2012.10.010","article-title":"Antimicrobial and\nCell-Penetrating Properties of Penetratin Analogs: Effect of Sequence\nand Secondary Structure","volume":"1828","author":"Bahnsen","year":"2013","journal-title":"Biochim. Biophys. 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Protoc."},{"issue":"26","key":"2026090117101391900_cit27","doi-asserted-by":"publisher","first-page":"8068","DOI":"10.1021\/ja072013j","article-title":"Covalent Capture\nof Phospho-Dependent Protein Oligomerization by Site-Specific Incorporation\nof a Diazirine Photo-Cross-Linker","volume":"129","author":"Vila-Perello\u0308","year":"2007","journal-title":"J. Am. Chem.\nSoc."},{"issue":"1","key":"2026090117101391900_cit28","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1038\/s41592-018-0260-3","article-title":"Deep Learning\nEnables de Novo Peptide Sequencing from Data-Independent-Acquisition\nMass Spectrometry","volume":"16","author":"Tran","year":"2019","journal-title":"Nat. Methods"},{"issue":"1","key":"2026090117101391900_cit29","doi-asserted-by":"publisher","first-page":"e1000270","DOI":"10.1371\/journal.ppat.1000270","article-title":"Rab11A-Controlled Assembly\nof the Inner Membrane Complex\nIs Required for Completion of Apicomplexan Cytokinesis","volume":"5","author":"Agop-Nersesian","year":"2009","journal-title":"PLoS Pathog."},{"issue":"3","key":"2026090117101391900_cit30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033845","article-title":"Subcellular Location, Phosphorylation\nand Assembly\ninto the Motor Complex of GAP45 during Plasmodium Falciparum Schizont\nDevelopment","volume":"7","author":"Ridzuan","year":"2012","journal-title":"PLoS One"}],"container-title":["ACS Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acschembio.0c00328","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/15\/6\/1313\/67382436\/acschembio.0c00328.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/15\/6\/1313\/67382436\/acschembio.0c00328.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T21:10:19Z","timestamp":1788297019000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acbcct\/article\/15\/6\/1313\/588863\/Peptide-Probes-for-Plasmodium-falciparum-MyoA-Tail"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,8]]},"references-count":30,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,5,8]]},"published-print":{"date-parts":[[2020,6,19]]}},"URL":"https:\/\/doi.org\/10.1021\/acschembio.0c00328","relation":{},"ISSN":["1554-8929","1554-8937"],"issn-type":[{"value":"1554-8929","type":"print"},{"value":"1554-8937","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,8]]}}}