{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T09:59:29Z","timestamp":1793095169266,"version":"4.5.0"},"reference-count":81,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T00:00:00Z","timestamp":1657238400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T00:00:00Z","timestamp":1657238400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T00:00:00Z","timestamp":1657238400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000057","name":"U.S. Department of Health and Human Services","doi-asserted-by":"publisher","award":["GM107036"],"award-info":[{"award-number":["GM107036"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>New antibiotics are needed as bacterial infections continue to be a leading cause of death, but efforts to develop compounds with promising antibacterial activity are hindered by a poor understanding of\ue5f8and limited strategies for elucidating\ue5f8their modes of action. We recently discovered a novel lasso peptide, ubonodin, that is active against opportunistic human lung pathogens from the Burkholderia cepacia complex (Bcc). Ubonodin inhibits RNA polymerase, but only select strains were susceptible, indicating that having a conserved cellular target does not guarantee activity. Given the cytoplasmic target, we hypothesized that cellular uptake of ubonodin determines susceptibility. Although Bcc strains harbor numerous nutrient uptake systems, these organisms lack close homologues of the single known lasso peptide membrane receptor, FhuA. Thus, a straightforward homology-driven approach failed to uncover the identity of the ubonodin transporter(s). Here, we used phenotype-guided comparative genomics to identify genes uniquely associated with ubonodin-susceptible Bcc strains, leading to the identification of PupB as the ubonodin outer membrane (OM) receptor in Burkholderia. The loss of PupB renders B. cepacia resistant to ubonodin, whereas expressing PupB sensitizes a resistant strain. We also examine how a conserved iron-regulated transcriptional pathway controls PupB to further tune ubonodin susceptibility. PupB is only the second lasso peptide OM receptor to be uncovered and the first outside of enterobacteria. Finally, we elucidate the full transport pathway for ubonodin by identifying its inner membrane receptor YddA in Burkholderia. Our work provides a complete picture of the mode of action of ubonodin and establishes a general framework for deciphering the transport pathways of other natural products with cytoplasmic targets.<\/jats:p>","DOI":"10.1021\/acschembio.2c00420","type":"journal-article","created":{"date-parts":[[2022,7,8]],"date-time":"2022-07-08T12:46:36Z","timestamp":1657284396000},"page":"2332-2343","source":"Crossref","is-referenced-by-count":23,"title":["Phenotype-Guided\nComparative Genomics Identifies the\nComplete Transport Pathway of the Antimicrobial Lasso Peptide Ubonodin\nin\n                    <i>Burkholderia<\/i>"],"prefix":"10.1021","volume":"17","author":[{"given":"Truc","family":"Do","sequence":"first","affiliation":[{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Thokkadam","sequence":"additional","affiliation":[{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Leach","sequence":"additional","affiliation":[{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5066-9691","authenticated-orcid":true,"given":"A. James","family":"Link","sequence":"additional","affiliation":[{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]},{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]},{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2022,7,8]]},"reference":[{"key":"2026081413093284300_cit1","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1039\/c2np20085f","article-title":"Ribosomally Synthesized\nand Post-Translationally Modified Peptide Natural Products: Overview\nand Recommendations for a Universal Nomenclature","volume":"30","author":"Arnison","year":"2012","journal-title":"Nat. Prod. Rep."},{"key":"2026081413093284300_cit2","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1039\/d0np00027b","article-title":"New Developments in RiPP Discovery, Enzymology and\nEngineering","volume":"38","author":"Montalba\u0301n-Lo\u0301pez","year":"2021","journal-title":"Nat. Prod. Rep."},{"key":"2026081413093284300_cit3","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1074\/jbc.rev119.006545","article-title":"The Manifold Roles of Microbial Ribosomal Peptide\u2013Based\nNatural Products in Physiology and Ecology","volume":"295","author":"Li","year":"2020","journal-title":"J. Biol. Chem."},{"key":"2026081413093284300_cit4","doi-asserted-by":"publisher","first-page":"kuab005","DOI":"10.1093\/jimb\/kuab005","article-title":"Mechanisms\nof Action of Ribosomally Synthesized and\nPosttranslationally Modified Peptides (RiPPs)","volume":"48","author":"Cao","year":"2021","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"2026081413093284300_cit5","doi-asserted-by":"publisher","first-page":"703","DOI":"10.1007\/s10295-020-02292-6","article-title":"How to Harness Biosynthetic\nGene Clusters of Lasso Peptides","volume":"47","author":"Kodani","year":"2020","journal-title":"J. Ind. Microbiol.\nBiotechnol."},{"key":"2026081413093284300_cit6","doi-asserted-by":"publisher","first-page":"1335","DOI":"10.1002\/cbic.201900707","article-title":"Discovery\nof Ubonodin, an Antimicrobial Lasso Peptide Active against Members\nof the Burkholderia cepacia Complex","volume":"21","author":"Cheung-Lee","year":"2020","journal-title":"Chembiochem"},{"key":"2026081413093284300_cit7","doi-asserted-by":"crossref","DOI":"10.5772\/67712","article-title":"Burkholderia cepacia Complex Infections\nAmong Cystic\nFibrosis Patients: Perspectives and Challenges","volume-title":"Progress in Understanding Cystic Fibrosis","author":"Leita\u0303o","year":"2017"},{"key":"2026081413093284300_cit8","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1038\/nrmicro1085","article-title":"The Multifarious,\nMultireplicon Burkholderia cepacia Complex","volume":"3","author":"Mahenthiralingam","year":"2005","journal-title":"Nat.\nRev. Microbiol."},{"key":"2026081413093284300_cit9","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/j.tim.2006.04.006","article-title":"Burkholderia cepacia Complex Species: Health Hazards\nand Biotechnological Potential","volume":"14","author":"Chiarini","year":"2006","journal-title":"Trends Microbiol."},{"key":"2026081413093284300_cit10","doi-asserted-by":"publisher","first-page":"1909","DOI":"10.1021\/acs.accounts.5b00156","article-title":"Lasso Peptides:\nAn Intriguing Class of Bacterial Natural Products","volume":"48","author":"Hegemann","year":"2015","journal-title":"Acc. Chem. Res."},{"key":"2026081413093284300_cit11","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1039\/c2np20070h","article-title":"Lasso Peptides : Structure, Function,\nBiosynthesis, and Engineering","volume":"29","author":"Maksimov","year":"2012","journal-title":"Nat. Prod. Rep."},{"key":"2026081413093284300_cit12","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1042\/bst20120176","article-title":"The Role of Bacterial Membrane Proteins in the Internalization of\nMicrocin MccJ25 and MccB17","volume":"40","author":"Mathavan","year":"2012","journal-title":"Biochem. Soc. Trans."},{"key":"2026081413093284300_cit13","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1007\/s10482-014-0148-x","article-title":"Time to Revisit Polyphasic Taxonomy","volume":"106","author":"Vandamme","year":"2014","journal-title":"Antonie van Leeuwenhoek"},{"key":"2026081413093284300_cit14","doi-asserted-by":"publisher","first-page":"1594","DOI":"10.3389\/fmicb.2020.01594","article-title":"Burkholderia\ncepacia Complex Taxon K: Where to Split?","volume":"11","author":"Depoorter","year":"2020","journal-title":"Front. Microbiol."},{"key":"2026081413093284300_cit15","doi-asserted-by":"publisher","first-page":"2803","DOI":"10.1093\/bioinformatics\/btn524","article-title":"The Burkholderia Genome Database:\nFacilitating Flexible Queries and Comparative Analyses","volume":"24","author":"Winsor","year":"2008","journal-title":"Bioinformatics"},{"key":"2026081413093284300_cit16","doi-asserted-by":"publisher","first-page":"3165","DOI":"10.1128\/jcm.38.9.3165-3173.2000","article-title":"DNA-Based Diagnostic Approaches for\nIdentification of Burkholderia cepacia Complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia\nstabilis, and Burkholderia cepacia Genomovars\nI and III","volume":"38","author":"Mahenthiralingam","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"2026081413093284300_cit17","doi-asserted-by":"publisher","first-page":"3427","DOI":"10.1128\/jcm.39.10.3427-3436.2001","article-title":"Taxonomy and Identification\nof the Burkholderia cepacia Complex","volume":"39","author":"Coenye","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"2026081413093284300_cit18","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1128\/cmr.00068-09","article-title":"The Changing\nMicrobial Epidemiology in Cystic Fibrosis","volume":"23","author":"LiPuma","year":"2010","journal-title":"Clin.\nMicrobiol. Rev."},{"key":"2026081413093284300_cit19","doi-asserted-by":"publisher","first-page":"1549","DOI":"10.1513\/annalsats.201906-443oc","article-title":"Epidemiology\nof Burkholderia Infections in People with Cystic\nFibrosis in Canada between 2000 and 2017","volume":"17","author":"Zlosnik","year":"2020","journal-title":"Ann.\nAm. Thorac. Soc."},{"key":"2026081413093284300_cit20","doi-asserted-by":"publisher","first-page":"7428","DOI":"10.1128\/jb.174.22.7428-7435.1992","article-title":"Microcin\n25, a Novel Antimicrobial Peptide Produced\nby Escherichia coli","volume":"174","author":"Salomo\u0301n","year":"1992","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit21","doi-asserted-by":"publisher","first-page":"4543","DOI":"10.1128\/jb.183.15.4543-4550.2001","article-title":"Escherichia coli RNA Polymerase Is\nthe Target of the Cyclopeptide\nAntibiotic Microcin J25","volume":"183","author":"Delgado","year":"2001","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit22","doi-asserted-by":"publisher","first-page":"50867","DOI":"10.1074\/jbc.m209425200","article-title":"Mutations of Bacterial RNA Polymerase Leading to Resistance\nto Microcin J25","volume":"277","author":"Yuzenkova","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2026081413093284300_cit23","doi-asserted-by":"publisher","first-page":"11446","DOI":"10.1021\/ja802966g","article-title":"Isolation and Structural Characterization of Capistruin,\na Lasso Peptide Predicted from the Genome Sequence of Burkholderia\nthailandensis E264","volume":"130","author":"Knappe","year":"2008","journal-title":"J. Am. Chem. Soc."},{"key":"2026081413093284300_cit24","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1016\/j.jmb.2011.02.060","article-title":"The Antibacterial Threaded-Lasso Peptide Capistruin\nInhibits Bacterial RNA Polymerase","volume":"412","author":"Kuznedelov","year":"2011","journal-title":"J. Mol. Biol."},{"key":"2026081413093284300_cit25","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.1073\/pnas.1817352116","article-title":"Structural Mechanism\nof Transcription Inhibition by\nLasso Peptides Microcin J25 and Capistruin","volume":"116","author":"Braffman","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026081413093284300_cit26","doi-asserted-by":"publisher","first-page":"7741","DOI":"10.1128\/jb.175.23.7741-7742.1993","article-title":"The FhuA Protein Is Involved in Microcin 25 Uptake","volume":"175","author":"Salomo\u0301n","year":"1993","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit27","doi-asserted-by":"publisher","first-page":"814","DOI":"10.1021\/acschembio.6b01154","article-title":"Acinetodin and Klebsidin, RNA Polymerase\nTargeting Lasso Peptides Produced by Human Isolates of Acinetobacter\ngyllenbergii and Klebsiella pneumoniae","volume":"12","author":"Metelev","year":"2017","journal-title":"ACS Chem. Biol."},{"key":"2026081413093284300_cit28","doi-asserted-by":"publisher","first-page":"8758","DOI":"10.1021\/acs.jafc.1c02659","article-title":"Discovery\nand Bioactivity of the Novel Lasso Peptide Microcin Y","volume":"69","author":"Li","year":"2021","journal-title":"J. Agric. Food Chem."},{"key":"2026081413093284300_cit29","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1146\/annurev.micro.112408.134247","article-title":"TonB-Dependent Transporters:\nRegulation, Structure, and Function","volume":"64","author":"Noinaj","year":"2010","journal-title":"Nat. Microbiol."},{"key":"2026081413093284300_cit30","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1139\/o10-141","article-title":"TonB or Not TonB:\nIs That the Question?","volume":"89","author":"Krewulak","year":"2011","journal-title":"Biochem. Cell Biol."},{"key":"2026081413093284300_cit31","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1039\/b906679a","article-title":"Chemistry and Biology of Siderophores","volume":"27","author":"Hider","year":"2010","journal-title":"Nat.\nProd. Rep."},{"key":"2026081413093284300_cit32","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00097-18","article-title":"TonB-Dependent\nReceptor Repertoire of Pseudomonas aeruginosa for\nUptake of Siderophore-Drug Conjugates","volume":"62","author":"Luscher","year":"2018","journal-title":"Antimicrob.\nAgents Chemother."},{"key":"2026081413093284300_cit33","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1016\/S0168-6445(03)00055-X","article-title":"Bacterial\nIron Homeostasis","volume":"27","author":"Andrews","year":"2003","journal-title":"FEMS Microbiol. Rev."},{"key":"2026081413093284300_cit34","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1007\/s10534-006-9065-4","article-title":"Iron Acquisition\nMechanisms of the Burkholderia cepacia Complex","volume":"20","author":"Thomas","year":"2007","journal-title":"BioMetals"},{"key":"2026081413093284300_cit35","doi-asserted-by":"publisher","first-page":"460","DOI":"10.3389\/fcimb.2017.00460","article-title":"Iron Acquisition\nMechanisms and Their Role in the Virulence\nof Burkholderia Species","volume":"7","author":"Butt","year":"2017","journal-title":"Front.\nCell. Infect. Microbiol."},{"key":"2026081413093284300_cit36","doi-asserted-by":"publisher","first-page":"33","DOI":"10.3390\/vaccines5040033","article-title":"Characterization\nof the Burkholderia\ncenocepacia tonB Mutant as a Potential Live Attenuated Vaccine","volume":"5","author":"Pradenas","year":"2017","journal-title":"Vaccines"},{"key":"2026081413093284300_cit37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003863","article-title":"Characterization\nof the Burkholderia mallei tonB Mutant and Its Potential\nas a Backbone Strain for Vaccine Development","volume":"9","author":"Mott","year":"2015","journal-title":"PLoS Neglected Trop. Dis."},{"key":"2026081413093284300_cit38","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/j.plasmid.2016.11.002","article-title":"An Efficient\nSystem for the Generation of Marked Genetic Mutants in Members of\nthe Genus Burkholderia","volume":"89","author":"Shastri","year":"2017","journal-title":"Plasmid"},{"key":"2026081413093284300_cit39","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00260-17","article-title":"The Essential\nGenome of Burkholderia\ncenocepacia H111","volume":"199","author":"Higgins","year":"2017","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit40","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.plasmid.2005.03.004","article-title":"An Expression Vector Containing a\nRhamnose-Inducible\nPromoter Provides Tightly Regulated Gene Expression in Burkholderia\ncenocepacia","volume":"54","author":"Cardona","year":"2005","journal-title":"Plasmid"},{"key":"2026081413093284300_cit41","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1111\/j.1365-2958.1993.tb01603.x","article-title":"Identification\nand\nCharacterization of the pupB Gene Encoding an Inducible\nFerric-pseudobactin Receptor of Pseudomonas putida WCS358","volume":"8","author":"Koster","year":"1993","journal-title":"Mol. Microbiol."},{"key":"2026081413093284300_cit42","doi-asserted-by":"publisher","first-page":"1177","DOI":"10.1046\/j.1365-2958.2002.03088.x","article-title":"Iron Transport and\nRegulation, Cell Signalling and Genomics: Lessons from Escherichia\ncoli and Pseudomonas","volume":"45","author":"Visca","year":"2002","journal-title":"Mol. Microbiol."},{"key":"2026081413093284300_cit43","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/s1369-5274(03)00022-5","article-title":"Regulation\nof the FecI-Type ECF Sigma\nFactor by Transmembrane Signalling","volume":"6","author":"Braun","year":"2003","journal-title":"Curr. Opin.\nMicrobiol."},{"key":"2026081413093284300_cit44","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1016\/j.femsre.2004.10.001","article-title":"Transmembrane Transcriptional\nControl (Surface Signalling)\nof the Escherichia coli Fec Type","volume":"29","author":"Braun","year":"2005","journal-title":"FEMS Microbiol. Rev."},{"key":"2026081413093284300_cit45","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/j.femsle.2005.09.043","article-title":"Genome-wide Expression Analysis of\nIron Regulation\nin Burkholderia pseudomallei and Burkholderia\nmallei Using DNA Microarrays","volume":"252","author":"Tuanyok","year":"2005","journal-title":"FEMS\nMicrobiol. Lett."},{"key":"2026081413093284300_cit46","doi-asserted-by":"publisher","first-page":"1568","DOI":"10.1038\/ismej.2013.36","article-title":"The Unexpected\nDiscovery of a Novel\nLow-Oxygen-Activated Locus for the Anoxic Persistence of Burkholderia\ncenocepacia","volume":"7","author":"Sass","year":"2013","journal-title":"ISME J."},{"key":"2026081413093284300_cit47","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1007\/s10534-015-9840-1","article-title":"Investigation\nof the Multifaceted Iron Acquisition Strategies of Burkholderia\ncenocepacia","volume":"28","author":"Tyrrell","year":"2015","journal-title":"BioMetals"},{"key":"2026081413093284300_cit48","doi-asserted-by":"publisher","first-page":"D295","DOI":"10.1093\/nar\/gkab1110","article-title":"PRODORIC: State-of-the-Art Database\nof Prokaryotic Gene Regulation","volume":"50","author":"Dudek","year":"2021","journal-title":"Nucleic Acids\nRes."},{"key":"2026081413093284300_cit49","doi-asserted-by":"publisher","first-page":"7194","DOI":"10.1128\/jb.177.24.7194-7201.1995","article-title":"Role of\nthe Ferric Uptake Regulator\nof Pseudomonas aeruginosa in the Regulation of Siderophores\nand Exotoxin A Expression: Purification and Activity on Iron-Regulated\nPromoters","volume":"177","author":"Ochsner","year":"1995","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit50","doi-asserted-by":"publisher","first-page":"1452","DOI":"10.1128\/jb.179.5.1452-1459.1997","article-title":"An Operon Containing fumC and sodA Encoding Fumarase C and Manganese\nSuperoxide Dismutase\nIs Controlled by the Ferric Uptake Regulator in Pseudomonas\naeruginosa: fur Mutants Produce Elevated\nAlginate Levels","volume":"179","author":"Hassett","year":"1997","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit51","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1099\/00221287-146-1-185","article-title":"Genetics\nand regulation of two distinct\nhaem-uptake systems, phu and has, in Pseudomonas aeruginosa","volume":"146","author":"Ochsner","year":"2000","journal-title":"Microbiology"},{"key":"2026081413093284300_cit52","doi-asserted-by":"publisher","first-page":"W202","DOI":"10.1093\/nar\/gkp335","article-title":"MEME Suite:\nTools for Motif Discovery and Searching","volume":"37","author":"Bailey","year":"2009","journal-title":"Nucleic\nAcids Res."},{"key":"2026081413093284300_cit53","doi-asserted-by":"publisher","first-page":"4118","DOI":"10.1093\/bioinformatics\/bty496","article-title":"Operon-Mapper: A Web\nServer for Precise Operon Identification in Bacterial and Archaeal\nGenomes","volume":"34","author":"Taboada","year":"2018","journal-title":"Bioinformatics"},{"key":"2026081413093284300_cit54","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1007\/BF00140109","article-title":"Ornibactins\ue5f8a New Family of\nSiderophores from Pseudomonas","volume":"6","author":"Stephan","year":"1993","journal-title":"BioMetals"},{"key":"2026081413093284300_cit55","doi-asserted-by":"publisher","first-page":"874","DOI":"10.1128\/iai.66.2.874-877.1998","article-title":"Siderophore Production\nby Cystic Fibrosis Isolates of Burkholderia cepacia","volume":"66","author":"Darling","year":"1998","journal-title":"Infect. Immun."},{"key":"2026081413093284300_cit56","doi-asserted-by":"publisher","first-page":"6554","DOI":"10.1128\/iai.68.12.6554-6560.2000","article-title":"Identification\nof a Siderophore Receptor Required for Ferric Ornibactin Uptake in Burkholderia cepacia","volume":"68","author":"Sokol","year":"2000","journal-title":"Infect. Immun."},{"key":"2026081413093284300_cit57","doi-asserted-by":"publisher","first-page":"6223","DOI":"10.1128\/jb.181.20.6223-6229.1999","article-title":"Opening the\nIron Box: Transcriptional Metalloregulation by the Fur Protein","volume":"181","author":"Escolar","year":"1999","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit58","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1007\/bf00383321","article-title":"Nucleotide Sequence of the Iron Regulatory\nGene Fur","volume":"200","author":"Scha\u0308ffer","year":"1985","journal-title":"Mol. Gen. Genet."},{"key":"2026081413093284300_cit59","doi-asserted-by":"publisher","first-page":"5471","DOI":"10.1021\/bi00391a039","article-title":"Ferric Uptake Regulation Protein Acts as a Repressor,\nEmploying Iron\u00ad(II) as a Cofactor to Bind the Operator of an Iron Transport\nOperon in Escherichia coli","volume":"26","author":"Bagg","year":"1987","journal-title":"Biochemistry"},{"key":"2026081413093284300_cit60","doi-asserted-by":"publisher","first-page":"1763","DOI":"10.1099\/mic.0.2007\/015537-0","article-title":"Pleiotropic Roles of\nIron-Responsive Transcriptional Regulator Fur\nin Burkholderia multivorans","volume":"154","author":"Yuhara","year":"2008","journal-title":"Microbiology"},{"key":"2026081413093284300_cit61","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0236405","article-title":"Low Iron-Induced Small\nRNA BrrF Regulates Central Metabolism\nand Oxidative Stress Responses in Burkholderia cenocepacia","volume":"15","author":"Sass","year":"2020","journal-title":"PloS One"},{"key":"2026081413093284300_cit62","doi-asserted-by":"publisher","first-page":"2624","DOI":"10.1128\/jb.169.6.2624-2630.1987","article-title":"Operator\nSequences of the Aerobactin Operon of Plasmid ColV-K30 Binding the\nFerric Uptake Regulation (fur) Repressor","volume":"169","author":"de\nLorenzo","year":"1987","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit63","doi-asserted-by":"publisher","first-page":"3323","DOI":"10.1128\/jb.177.11.3323-3325.1995","article-title":"The Peptide Antibiotic Microcin 25 Is Imported through\nthe TonB Pathway and the SbmA Protein","volume":"177","author":"Salomo\u0301n","year":"1995","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit64","doi-asserted-by":"publisher","first-page":"6822","DOI":"10.1074\/jbc.ra118.006494","article-title":"Discovery\nand Structure of the Antimicrobial Lasso Peptide Citrocin","volume":"294","author":"Cheung-Lee","year":"2019","journal-title":"J. Biol. Chem."},{"key":"2026081413093284300_cit65","doi-asserted-by":"publisher","first-page":"982","DOI":"10.4014\/jmb.2003.03003","article-title":"Cloning and Functional Characterization\nof Putative Escherichia coli ABC Multidrug Efflux\nTransporter YddA","volume":"30","author":"Feng","year":"2020","journal-title":"J. Microbiol. Biotechnol."},{"key":"2026081413093284300_cit66","doi-asserted-by":"publisher","first-page":"1608","DOI":"10.1093\/bioinformatics\/btq249","article-title":"PSORTb 3.0: Improved Protein Subcellular Localization\nPrediction\nwith Refined Localization Subcategories and Predictive Capabilities\nfor All Prokaryotes","volume":"26","author":"Yu","year":"2010","journal-title":"Bioinformatics"},{"key":"2026081413093284300_cit67","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1016\/j.jmb.2004.03.016","article-title":"A Combined\nTransmembrane Topology\nand Signal Peptide Prediction Method","volume":"338","author":"Ka\u0308ll","year":"2004","journal-title":"J. Mol.\nBiol."},{"key":"2026081413093284300_cit68","doi-asserted-by":"publisher","first-page":"W429","DOI":"10.1093\/nar\/gkm256","article-title":"Advantages\nof Combined Transmembrane\nTopology and Signal Peptide Prediction\ue5f8the Phobius Web Server","volume":"35","author":"Ka\u0308ll","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"2026081413093284300_cit69","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1038\/nchembio.1499","article-title":"Structural Basis for Hijacking Siderophore\nReceptors by Antimicrobial\nLasso Peptides","volume":"10","author":"Mathavan","year":"2014","journal-title":"Nat. Chem. Biol."},{"key":"2026081413093284300_cit70","doi-asserted-by":"publisher","first-page":"1652","DOI":"10.1111\/j.1462-2920.2008.01576.x","article-title":"A System for the Construction of\nTargeted Unmarked Gene Deletions in the Genus Burkholderia","volume":"10","author":"Flannagan","year":"2008","journal-title":"Environ. Microbiol."},{"key":"2026081413093284300_cit71","doi-asserted-by":"publisher","first-page":"4498","DOI":"10.1128\/aem.00531-08","article-title":"Genetic Tools for\nAllelic Replacement in Burkholderia Species","volume":"74","author":"Barrett","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"2026081413093284300_cit72","doi-asserted-by":"publisher","first-page":"2372","DOI":"10.1021\/acssynbio.9b00232","article-title":"A Broad-Host-Range\nCRISPRi Toolkit for Silencing Gene Expression in Burkholderia","volume":"8","author":"Hogan","year":"2019","journal-title":"ACS Synth. Biol."},{"key":"2026081413093284300_cit73","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00647-21","article-title":"Improved\nDynamic\nRange of a Rhamnose-Inducible Promoter for Gene Expression in Burkholderia Spp","volume":"87","author":"Hogan","year":"2021","journal-title":"Appl. Environ. Microbiol."},{"key":"2026081413093284300_cit74","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00479-17","article-title":"The Small Protein HemP Is a Transcriptional\nActivator for the Hemin\nUptake Operon in Burkholderia multivorans ATCC 17616","volume":"83","author":"Sato","year":"2017","journal-title":"Appl. Environ. Microbiol."},{"key":"2026081413093284300_cit75","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1111\/j.1574-6968.2004.tb09634.x","article-title":"Inhibition\nof Salmonella enterica Serovars by Microcin J25","volume":"236","author":"Vincent","year":"2004","journal-title":"FEMS Microbiol. Lett."},{"key":"2026081413093284300_cit76","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abj5363","article-title":"Molecular Mechanism\nof SbmA, a Promiscuous Transporter Exploited by Antimicrobial Peptides","volume":"7","author":"Ghilarov","year":"2021","journal-title":"Sci. Adv."},{"key":"2026081413093284300_cit77","doi-asserted-by":"publisher","first-page":"5343","DOI":"10.1128\/jb.00818-13","article-title":"Functional Characterization of SbmA, a Bacterial Inner\nMembrane Transporter Required for Importing the Antimicrobial Peptide\nBac7\u00ad(1-35)","volume":"195","author":"Runti","year":"2013","journal-title":"J. Bacteriol."},{"key":"2026081413093284300_cit78","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1186\/1471-2105-10-421","article-title":"BLAST+: Architecture and Applications","volume":"10","author":"Camacho","year":"2009","journal-title":"BMC Bioinf."},{"key":"2026081413093284300_cit79","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1159\/000235768","article-title":"The Genus Burkholderia: Analysis of 56 Genomic Sequences","volume":"6","author":"Ussery","year":"2009","journal-title":"Genome\nDyn."},{"key":"2026081413093284300_cit80","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.1093\/bioinformatics\/btp324","article-title":"Fast and Accurate Short\nRead Alignment with Burrows\u2013Wheeler\nTransform","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"2026081413093284300_cit81","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated Estimation\nof Fold Change\nand Dispersion for RNA-Seq Data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."}],"container-title":["ACS Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acschembio.2c00420","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/17\/8\/2332\/3732093\/cb2c00420.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/17\/8\/2332\/3732093\/cb2c00420.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T17:54:44Z","timestamp":1786730084000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acbcct\/article\/17\/8\/2332\/384825\/Phenotype-Guided-Comparative-Genomics-Identifies"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,8]]},"references-count":81,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,7,8]]},"published-print":{"date-parts":[[2022,8,19]]}},"URL":"https:\/\/doi.org\/10.1021\/acschembio.2c00420","relation":{},"ISSN":["1554-8929","1554-8937"],"issn-type":[{"value":"1554-8929","type":"print"},{"value":"1554-8937","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,8]]}}}