{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T15:47:43Z","timestamp":1792079263047,"version":"4.4.0"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2027,8,13]],"date-time":"2027-08-13T00:00:00Z","timestamp":1818115200000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Targeted protein degradation (TPD), including proteolysis targeting chimeras (PROTACs) and molecular glue degraders (MGDs), is a promising therapeutic approach. However, systematic discovery of such small molecules remains a major challenge. Here, we present PhenoDEL, a novel phenotypic DNA-encoded library (DEL) screening platform that integrates one-bead one-compound DEL (OBOC-DEL) with the Beacon optofluidic system for single-cell analysis. By coculturing individual OBOC-DEL beads and engineered reporter cells in nanoliter-scale chambers, PhenoDEL enables time-resolved, single-cell phenotypic evaluation and direct linkage between compound identity and intracellular response. As a proof-of-concept, we demonstrate discrimination of active and inactive on-bead compounds using an FKBP12F36V-EGFP degradation reporter in PC-3 cells, followed by DNA barcode decoding.<\/jats:p>","DOI":"10.1021\/acschembio.6c00234","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T01:02:01Z","timestamp":1786669321000},"page":"2157-2168","source":"Crossref","is-referenced-by-count":0,"title":["PhenoDEL: A Novel\nScreening Strategy Based on Intracellular\nProtein Degradation Activity"],"prefix":"10.1021","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6425-3498","authenticated-orcid":true,"given":"Yuichi","family":"Onda","sequence":"first","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6011-387X","authenticated-orcid":true,"given":"Yurika","family":"Ochi","sequence":"additional","affiliation":[{"name":"Nikon Corporation , ,","place":["Tokyo, Japan, 140-8601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Araki","sequence":"additional","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Kageoka-Takahashi","sequence":"additional","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzo","family":"Takeda","sequence":"additional","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Yamada","sequence":"additional","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehiko","family":"Ueda","sequence":"additional","affiliation":[{"name":"Nikon Corporation , ,","place":["Tokyo, Japan, 140-8601"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Ohno","sequence":"additional","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5878-8791","authenticated-orcid":true,"given":"Minoru","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiki","family":"Sakai","sequence":"additional","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2496-6179","authenticated-orcid":true,"given":"Miki","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihito","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Tanabe Pharma Corporation , ,","place":["Kanagawa, Japan, 227-0033"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"2026101511063845800_cit1","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1038\/s41589-019-0362-y","article-title":"Development of targeted protein degradation therapeutics","volume":"15","author":"Chamberlain","year":"2019","journal-title":"Nat. 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P.; Buckley, D.\n          Targeted\nprotein degradation\nto attenuate adoptive T-cell therapy associated adverse inflammatory\nresponses. WO 2017\/024318, 2017."},{"key":"2026101511063845800_cit39","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.chembiol.2019.12.006","article-title":"Exploring targeted degradation strategy for oncogenic\nKRAS G12C","volume":"27","author":"Zeng","year":"2020","journal-title":"Cell Chem. Biol."},{"key":"2026101511063845800_cit40","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1038\/nrd3368","article-title":"Impact\nof high-throughput screening in biomedical research","volume":"10","author":"Macarron","year":"2011","journal-title":"Nat. Rev. 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Oncol."},{"key":"2026101511063845800_cit44","doi-asserted-by":"publisher","first-page":"1310002","DOI":"10.3389\/fmmed.2024.1310002","article-title":"Current\nadvances in experimental\nand computational approaches to enhance CAR T cell manufacturing protocols\nand improve clinical efficacy","volume":"4","author":"Colina","year":"2024","journal-title":"Front. Mol. Med."},{"key":"2026101511063845800_cit45","doi-asserted-by":"publisher","first-page":"2901","DOI":"10.1039\/D1LC00389E","article-title":"High-throughput optofluidic screening\nfor improved\nmicrobial cell factories via real-time micron-scale productivity monitoring","volume":"21","author":"Rienzo","year":"2021","journal-title":"Lab Chip"}],"container-title":["ACS Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acschembio.6c00234","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/21\/9\/2157\/68550966\/cb-2026-00234s.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/21\/9\/2157\/68550966\/cb-2026-00234s.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T15:20:37Z","timestamp":1792077637000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acbcct\/article\/21\/9\/2157\/5254988\/PhenoDEL-A-Novel-Screening-Strategy-Based-on"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,13]]},"references-count":45,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,8,13]]},"published-print":{"date-parts":[[2026,9,18]]}},"URL":"https:\/\/doi.org\/10.1021\/acschembio.6c00234","relation":{},"ISSN":["1554-8929","1554-8937"],"issn-type":[{"value":"1554-8929","type":"print"},{"value":"1554-8937","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,13]]}}}