{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T20:43:05Z","timestamp":1793133785644,"version":"4.5.0"},"reference-count":67,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/T028904\/1"],"award-info":[{"award-number":["MR\/T028904\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["278001972-TRR186 TP24"],"award-info":[{"award-number":["278001972-TRR186 TP24"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Forschung, Technologie und Raumfahrt","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100014690","name":"Ministerium f\u00fcr Kultur und Wissenschaft des Landes Nordrhein-Westfalen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014690","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Inositols are a family of cyclic sugar alcohols comprising nine stereoisomers. myo-Inositol is the most abundant isomer found in humans and has been studied most extensively. It plays an important role in osmoregulation and is incorporated into membrane-anchored phosphatidylinositols. scyllo-Inositol is the second most abundant inositol isomer in the human brain, and aberrant concentrations are associated with various diseases; however, its biological functions remain unclear. Here, the development and application of [13C6]scyllo-inositol as an isotopic tracer to study its metabolism are reported. A concise and robust synthetic route was established to obtain [13C6]scyllo-inositol from [13C6]myo-inositol in good yield. The uptake of [13C6]scyllo-inositol at concentrations used in clinical trials and the responses of endogenous inositol isomers were measured in multiple immortalized mammalian cell lines by hydrophilic liquid interaction (HILIC)-MS\/MS. [13C6]scyllo-Inositol proved to be a versatile isotopic tracer when coupled with mass spectrometry (MS)-based lipidomics and 2D nuclear magnetic resonance (NMR) experiments. These experiments provide evidence that scyllo-inositol is incorporated into phosphatidylinositols in different immortalized mammalian cell lines and suggest a previously underappreciated role of scyllo-inositol. Future research utilizing [13C6]scyllo-inositol can elucidate scyllo-inositol metabolism and its role in physiological and diseased states.<\/jats:p>","DOI":"10.1021\/acschembio.6c00409","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:00:22Z","timestamp":1788253222000},"page":"2232-2243","source":"Crossref","is-referenced-by-count":1,"title":["Isotopic Tracing of [13C6]\n                    <i>scyllo<\/i>\n                    -Inositol Uncovers Its Incorporation into Phosphatidylinositols in Immortalized Mammalian Cells"],"prefix":"10.1021","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4964-585X","authenticated-orcid":true,"given":"Meike M.","family":"Amma","sequence":"first","affiliation":[{"name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP) , Robert-R\u00f6ssle-Str. 10 , ,","place":["Berlin, Germany, 13125"]},{"name":"Humboldt-Universit\u00e4t zu Berlin , , Brook-Taylor-Str. 2 , ,","place":["Berlin, Germany, 12489"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laxmikanth","family":"Kollipara","sequence":"additional","affiliation":[{"name":"Leibniz-Institut f\u00fcr Analytische Wissenschaften-ISAS e.V. , ,","place":["Dortmund, Germany, 44227"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5282-1826","authenticated-orcid":true,"given":"Tobias","family":"D\u00fcrr-Mayer","sequence":"additional","affiliation":[{"name":"University of Freiburg , , Albertstrasse 21 , ,","place":["Freiburg, Germany, 79104"]},{"name":"University of Freiburg , , ,","place":["Freiburg, Germany, 79104"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6271-9204","authenticated-orcid":true,"given":"Josefin","family":"Streeck","sequence":"additional","affiliation":[{"name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP) , Robert-R\u00f6ssle-Str. 10 , ,","place":["Berlin, Germany, 13125"]},{"name":"Humboldt-Universit\u00e4t zu Berlin , , Brook-Taylor-Str. 2 , ,","place":["Berlin, Germany, 12489"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9968-9327","authenticated-orcid":true,"given":"Peter","family":"Schmieder","sequence":"additional","affiliation":[{"name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP) , Robert-R\u00f6ssle-Str. 10 , ,","place":["Berlin, Germany, 13125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4351-0081","authenticated-orcid":true,"given":"Adolfo","family":"Saiardi","sequence":"additional","affiliation":[{"name":"University College London , , ,","place":["London, U.K., WC1E 6BT"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1025-9484","authenticated-orcid":true,"given":"Henning J.","family":"Jessen","sequence":"additional","affiliation":[{"name":"University of Freiburg , , Albertstrasse 21 , ,","place":["Freiburg, Germany, 79104"]},{"name":"University of Freiburg , , ,","place":["Freiburg, Germany, 79104"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3779-8071","authenticated-orcid":true,"given":"Sven","family":"Heiles","sequence":"additional","affiliation":[{"name":"Leibniz-Institut f\u00fcr Analytische Wissenschaften-ISAS e.V. , ,","place":["Dortmund, Germany, 44227"]},{"name":"University of Duisburg-Essen , , ,","place":["Essen, Germany, 45141"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0798-946X","authenticated-orcid":true,"given":"Dorothea","family":"Fiedler","sequence":"additional","affiliation":[{"name":"Leibniz-Forschungsinstitut f\u00fcr Molekulare Pharmakologie (FMP) , Robert-R\u00f6ssle-Str. 10 , ,","place":["Berlin, Germany, 13125"]},{"name":"Humboldt-Universit\u00e4t zu Berlin , , Brook-Taylor-Str. 2 , ,","place":["Berlin, Germany, 12489"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"issue":"28","key":"2026101511071421200_cit1","doi-asserted-by":"publisher","first-page":"7871","DOI":"10.1007\/s00216-020-02919-8","article-title":"Plasma and urinary inositol isomer profiles measured by UHPLC-MS\/MS reveal differences in scyllo-inositol levels between non-pregnant and pregnant women","volume":"412","author":"Monnard","year":"2020","journal-title":"Anal. Bioanal. Chem."},{"issue":"1","key":"2026101511071421200_cit2","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/0008-6215(94)00253-C","article-title":"A simple synthesis of cis-inositol","volume":"266","author":"Angyal","year":"1995","journal-title":"Carbohydr. Res."},{"issue":"11","key":"2026101511071421200_cit3","doi-asserted-by":"publisher","first-page":"936","DOI":"10.3390\/biology13110936","article-title":"Myo-Inositol and Its Derivatives: Their Roles in the Challenges of Infertility","volume":"13","author":"Placidi","year":"2024","journal-title":"Biology"},{"issue":"1","key":"2026101511071421200_cit4","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1002\/nbm.927","article-title":"Scyllo-inositol in normal aging human brain: 1H magnetic resonance spectroscopy study at 4 Tesla","volume":"18","author":"Kaiser","year":"2005","journal-title":"NMR Biomed."},{"key":"2026101511071421200_cit5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbior.2022.100921","article-title":"Regulations of myo-inositol homeostasis: Mechanisms, implications, and perspectives","volume":"87","author":"Su","year":"2023","journal-title":"Adv. Biol. Regul."},{"issue":"10","key":"2026101511071421200_cit6","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1016\/j.febslet.2015.03.012","article-title":"Inositol transport proteins","volume":"589","author":"Schneider","year":"2015","journal-title":"FEBS Lett."},{"issue":"16","key":"2026101511071421200_cit7","doi-asserted-by":"publisher","first-page":"4467","DOI":"10.1093\/emboj\/20.16.4467","article-title":"Identification of a mammalian H+-myo-inositol symporter expressed predominantly in the brain","volume":"20","author":"Uldry","year":"2001","journal-title":"EMBO J."},{"issue":"11","key":"2026101511071421200_cit8","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/j.tibs.2024.08.005","article-title":"The inositol phosphate signalling network in physiology and disease","volume":"49","author":"Kim","year":"2024","journal-title":"Trends Biochem. Sci."},{"issue":"11","key":"2026101511071421200_cit9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1013680","article-title":"A linear pathway for inositol pyrophosphate metabolism revealed by 18O labeling and model reduction","volume":"21","author":"Hermes","year":"2025","journal-title":"PLoS Comput. Biol."},{"issue":"3","key":"2026101511071421200_cit10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2024.105757","article-title":"Phosphoinositide switches in cell physiology - From molecular mechanisms to disease","volume":"300","author":"Lolicato","year":"2024","journal-title":"J. Biol. Chem."},{"issue":"2","key":"2026101511071421200_cit11","doi-asserted-by":"publisher","first-page":"342","DOI":"10.1016\/j.neuroimage.2011.12.038","article-title":"The neurochemical profile quantified by in vivo 1H NMR spectroscopy","volume":"61","author":"Duarte","year":"2012","journal-title":"NeuroImage"},{"issue":"28","key":"2026101511071421200_cit12","doi-asserted-by":"publisher","first-page":"18514","DOI":"10.1039\/C5CP02690C","article-title":"Structures, stability and hydrogen bonding in inositol conformers","volume":"17","author":"Siddiqui","year":"2015","journal-title":"Phys. Chem. Chem. Phys"},{"issue":"2","key":"2026101511071421200_cit13","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1002\/mrm.1910390220","article-title":"Identification of a high concentration of scyllo-inositol in the brain of a healthy human subject using 1H- and 13C-NMR","volume":"39","author":"Seaquist","year":"1998","journal-title":"Magn. Reson. Med."},{"issue":"1","key":"2026101511071421200_cit14","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/s10334-004-0044-x","article-title":"High cerebral scyllo-inositol: a new marker of brain metabolism disturbances induced by chronic alcoholism","volume":"17","author":"Viola","year":"2004","journal-title":"Magn. Reson. Mater. Phys."},{"issue":"1","key":"2026101511071421200_cit15","doi-asserted-by":"publisher","DOI":"10.1212\/NXI.0000000000000185","article-title":"1H-NMR analysis provides a metabolomic profile of patients with multiple sclerosis","volume":"3","author":"Cocco","year":"2016","journal-title":"Neurol. Neuroimmunol. Neuroinflammation"},{"issue":"2","key":"2026101511071421200_cit16","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/j.ejca.2012.09.002","article-title":"Magnetic resonance spectroscopy metabolite profiles predict survival in paediatric brain tumours","volume":"49","author":"Wilson","year":"2013","journal-title":"Eur. J. Cancer"},{"issue":"20","key":"2026101511071421200_cit17","doi-asserted-by":"publisher","first-page":"4438","DOI":"10.1016\/j.cell.2023.08.005","article-title":"Early Alzheimer\u2019s disease pathology in human cortex involves transient cell states","volume":"186","author":"Gazestani","year":"2023","journal-title":"Cell"},{"issue":"24","key":"2026101511071421200_cit18","doi-asserted-by":"publisher","first-page":"18495","DOI":"10.1074\/jbc.M906994199","article-title":"Inositol Stereoisomers Stabilize an Oligomeric Aggregate of Alzheimer Amyloid \u03b2 Peptide and Inhibit A\u03b2-induced Toxicity*","volume":"275","author":"McLaurin","year":"2000","journal-title":"J. Biol. Chem."},{"issue":"10","key":"2026101511071421200_cit19","doi-asserted-by":"publisher","first-page":"1629","DOI":"10.1016\/j.bbadis.2012.07.003","article-title":"Inhibition of amyloid-beta peptide aggregation rescues the autophagic deficits in the TgCRND8 mouse model of Alzheimer disease","volume":"1822","author":"Lai","year":"2012","journal-title":"Biochim. Biophys. Acta, Mol. Basis Dis."},{"issue":"2","key":"2026101511071421200_cit20","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1002\/cpdd.14","article-title":"Pharmacokinetic Profile of Orally Administered Scyllo-Inositol (Elnd005) in Plasma, Cerebrospinal Fluid and Brain, and Corresponding Effect on Amyloid-Beta in Healthy Subjects","volume":"2","author":"Liang","year":"2013","journal-title":"Clin. Pharmacol. Drug Dev."},{"issue":"4S_Part_11","key":"2026101511071421200_cit21","doi-asserted-by":"publisher","first-page":"P323","DOI":"10.1016\/j.jalz.2009.04.526","article-title":"P2-213: Oral amyloid anti-aggregating agent ELND005 is measurable in CSF and brain of healthy adult men","volume":"5","author":"Garzone","year":"2009","journal-title":"Alzheimer's Dementia"},{"issue":"1_supplement","key":"2026101511071421200_cit22","doi-asserted-by":"publisher","first-page":"P04.194","DOI":"10.1212\/WNL.78.1_MeetingAbstracts.P04.194","article-title":"Responders to ELND005 (Scyllo-Inositol) Demonstrate Clinical and Biomarker Characteristics Consistent with Mild Alzheimer's Disease (AD): Data from a 78-Week Phase 2 Study in Mild to Moderate (M\/M) AD (P04.194)","volume":"78","author":"Porsteinsson","year":"2012","journal-title":"Neurology"},{"issue":"13","key":"2026101511071421200_cit23","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1212\/WNL.0b013e3182309fa5","article-title":"A phase 2 randomized trial of ELND005, scyllo-inositol, in mild to moderate Alzheimer disease","volume":"77","author":"Salloway","year":"2011","journal-title":"Neurology"},{"key":"2026101511071421200_cit24","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2022.102739","article-title":"Isotope tracing in health and disease","volume":"76","author":"Dong","year":"2022","journal-title":"Curr. Opin. Biotechnol."},{"issue":"9","key":"2026101511071421200_cit25","doi-asserted-by":"publisher","first-page":"3312","DOI":"10.1021\/ac103038s","article-title":"Carbon Isotopes Profiles of Human Whole Blood, Plasma, Red Blood Cells, Urine and Feces for Biological\/Biomedical 14C-Accelerator Mass Spectrometry Applications","volume":"83","author":"Kim","year":"2011","journal-title":"Anal. Chem."},{"issue":"4","key":"2026101511071421200_cit26","doi-asserted-by":"publisher","first-page":"511","DOI":"10.4155\/bio.13.348","article-title":"Stable Isotope-Labeling Studies in Metabolomics: New Insights Into Structure and Dynamics of Metabolic Networks","volume":"6","author":"Chokkathukalam","year":"2014","journal-title":"Bioanalysis"},{"issue":"3","key":"2026101511071421200_cit27","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.jbiotec.2009.07.010","article-title":"Evaluation of 13C isotopic tracers for metabolic flux analysis in mammalian cells","volume":"144","author":"Metallo","year":"2009","journal-title":"J. Biotechnol."},{"issue":"12","key":"2026101511071421200_cit28","doi-asserted-by":"publisher","first-page":"3303","DOI":"10.1038\/s44318-025-00450-z","article-title":"Approaches to stable isotope tracing and in vivo metabolomics in the cancer clinic","volume":"44","author":"Faubert","year":"2025","journal-title":"EMBO J."},{"issue":"11","key":"2026101511071421200_cit29","doi-asserted-by":"publisher","first-page":"5123","DOI":"10.1038\/s41596-021-00605-2","article-title":"Stable isotope tracing to assess tumor metabolism in vivo","volume":"16","author":"Faubert","year":"2021","journal-title":"Nat. Protoc."},{"issue":"7","key":"2026101511071421200_cit30","doi-asserted-by":"publisher","first-page":"1812","DOI":"10.1038\/s41564-024-01698-7","article-title":"Phytate metabolism is mediated by microbial cross-feeding in the gut microbiota","volume":"9","author":"De Vos","year":"2024","journal-title":"Nat. Microbiol."},{"issue":"20","key":"2026101511071421200_cit31","doi-asserted-by":"publisher","first-page":"5267","DOI":"10.1039\/C9SC00151D","article-title":"Harnessing 13C-labeled myo-inositol to interrogate inositol phosphate messengers by NMR","volume":"10","author":"Harmel","year":"2019","journal-title":"Chem. Sci."},{"issue":"1","key":"2026101511071421200_cit32","doi-asserted-by":"publisher","first-page":"6035","DOI":"10.1038\/s41467-020-19928-x","article-title":"Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry","volume":"11","author":"Qiu","year":"2020","journal-title":"Nat. Commun."},{"issue":"12","key":"2026101511071421200_cit33","doi-asserted-by":"publisher","first-page":"1683","DOI":"10.1021\/acscentsci.2c01032","article-title":"Stable Isotopomers of myo-Inositol Uncover a Complex MINPP1-Dependent Inositol Phosphate Network","volume":"8","author":"Nguyen Trung","year":"2022","journal-title":"ACS Cent. Sci."},{"issue":"S1","key":"2026101511071421200_cit34","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1002\/sia.5659","article-title":"Quantitative imaging of inositol distribution in yeast using multi-isotope imaging mass spectrometry (MIMS)","volume":"46","author":"Saiardi","year":"2014","journal-title":"Surf. Interface Anal."},{"issue":"15","key":"2026101511071421200_cit35","doi-asserted-by":"publisher","first-page":"3799","DOI":"10.1021\/ja9840293","article-title":"Synthesis of 1,2,3,4-Tetrahydroxybenzene from d-Glucose: Exploiting myo-Inositol as a Precursor to Aromatic Chemicals","volume":"121","author":"Hansen","year":"1999","journal-title":"J. Am. Chem. Soc."},{"issue":"2","key":"2026101511071421200_cit36","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1021\/jo01096a628","article-title":"Communications - Scyllitol Diborate","volume":"23","author":"Weissbach","year":"1958","journal-title":"J. Org. Chem."},{"issue":"2","key":"2026101511071421200_cit37","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/0008-6215(87)80055-1","article-title":"Preparative-scale separation by anion-exchange chromatography of six per-C-deuterated inositol epimers produced during C-1H-C-2H exchange reactions with raney nickel in deuterium oxide","volume":"166","author":"Sasaki","year":"1987","journal-title":"Carbohydr. Res."},{"issue":"5","key":"2026101511071421200_cit38","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1134\/S1990519X16050072","article-title":"A172 and T98G cell lines characteristics","volume":"10","author":"Kiseleva","year":"2016","journal-title":"Cell Tissue Biol."},{"issue":"24","key":"2026101511071421200_cit39","doi-asserted-by":"publisher","first-page":"20164","DOI":"10.1074\/jbc.M111.337196","article-title":"1H NMR Metabolomics Analysis of Glioblastoma Subtypes: Correlation between Metabolomics and GeneExpression Characteristics","volume":"287","author":"Cuperlovic-Culf","year":"2012","journal-title":"J. Biol. Chem."},{"issue":"2","key":"2026101511071421200_cit40","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1158\/2159-8290.CD-20-1849","article-title":"SLC5A3-Dependent Myo-inositol Auxotrophy in Acute Myeloid Leukemia","volume":"12","author":"Wei","year":"2022","journal-title":"Cancer Discovery"},{"issue":"4","key":"2026101511071421200_cit41","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/0009-8981(73)90036-3","article-title":"Gas chromatographic measurement of myo-inositol in human blood, cerebrospinal fluid and seminal fluid","volume":"45","author":"Lewin","year":"1973","journal-title":"Clin. Chim. Acta"},{"issue":"11","key":"2026101511071421200_cit42","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1042\/BCJ20253028","article-title":"Basic features of cellular inositol metabolism as revealed by a newly developed LC-MS method","volume":"482","author":"Su","year":"2025","journal-title":"Biochem. J."},{"issue":"4S_Part_21","key":"2026101511071421200_cit43","doi-asserted-by":"publisher","first-page":"S783","DOI":"10.1016\/j.jalz.2013.08.140","article-title":"P4-359: Effects of oral ELND005 (Scyllo-inositol) on brain scyllo-inositol and myo-inositol levels: Spectroscopy results from a phase 2 study in MILD-TO-MODERATE Alzheimer's disease","volume":"8","author":"Liang","year":"2012","journal-title":"Alzheimer's Dementia"},{"issue":"3","key":"2026101511071421200_cit44","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1111\/j.1550-7408.2003.tb00111.x","article-title":"Identification of the Inositol Isomers Present in Tetrahymena","volume":"50","author":"Kersting","year":"2003","journal-title":"J Eukaryotic Microbiol."},{"key":"2026101511071421200_cit45","doi-asserted-by":"publisher","first-page":"3601","DOI":"10.3390\/molecules26123601","article-title":"Novel Substrates for Kinases Involved in the Biosynthesis of Inositol Pyrophosphates and Their Enhancement of ATPase Activity of a Kinase","volume":"26","author":"Mohanrao","year":"2021","journal-title":"Molecules"},{"issue":"37","key":"2026101511071421200_cit46","doi-asserted-by":"publisher","first-page":"11114","DOI":"10.1021\/bi010817k","article-title":"Measurement of myo-Inositol Turnover in Phosphatidylinositol: Description of a Model and Mass Spectrometric Method for Cultured Cortical Neurons","volume":"40","author":"Chikhale","year":"2001","journal-title":"Biochemistry"},{"issue":"4","key":"2026101511071421200_cit47","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.1104\/pp.113.4.1385","article-title":"Novel Phosphoinositides in Barley Aleurone Cells (Additional Evidence for the Presence of Phosphatidyl-scyllo-Inositol)","volume":"113","author":"Narasimhan","year":"1997","journal-title":"Plant Physiol."},{"issue":"4","key":"2026101511071421200_cit48","doi-asserted-by":"publisher","first-page":"937","DOI":"10.5740\/jaoacint.CS2011_18","article-title":"Determination of Myo-Inositol (Free and Bound as Phosphatidylinositol) in Infant Formula and Adult Nutritionals by Liquid Chromatography\/Pulsed Amperometry with Column Switching: First Action 2011.18","volume":"95","author":"Schimpf","year":"2012","journal-title":"J. AOAC Int."},{"issue":"4","key":"2026101511071421200_cit49","doi-asserted-by":"publisher","first-page":"1018","DOI":"10.1111\/j.1471-4159.1987.tb05619.x","article-title":"Reduced Phosphoinositide Concentrations in Anterior Temporal Cortex of Alzheimer-Diseased Brains","volume":"48","author":"Stokes","year":"1987","journal-title":"J. Neurochem."},{"issue":"5","key":"2026101511071421200_cit50","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1194\/jlr.D700041-JLR200","article-title":"Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics*","volume":"49","author":"Matyash","year":"2008","journal-title":"J. Lipid Res."},{"issue":"11","key":"2026101511071421200_cit51","doi-asserted-by":"publisher","first-page":"6257","DOI":"10.1021\/acs.analchem.7b01259","article-title":"Lipostar, a Comprehensive Platform-Neutral Cheminformatics Tool for Lipidomics","volume":"89","author":"Goracci","year":"2017","journal-title":"Anal. Chem."},{"issue":"18","key":"2026101511071421200_cit52","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021110038","article-title":"Acyl chain selection couples the consumption and synthesis of phosphoinositides","volume":"41","author":"Barneda","year":"2022","journal-title":"EMBO J."},{"issue":"7","key":"2026101511071421200_cit53","doi-asserted-by":"publisher","first-page":"2843","DOI":"10.1007\/s00216-010-4184-8","article-title":"Elucidation of the fragmentation pathways of different phosphatidylinositol phosphate species (PIPx) using IRMPD implemented on a FT-ICR MS","volume":"398","author":"Zehethofer","year":"2010","journal-title":"Anal. Bioanal. Chem."},{"issue":"6","key":"2026101511071421200_cit54","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1039\/C5AY00776C","article-title":"Fragmentation patterns of five types of phospholipids by ultra-high-performance liquid chromatography electrospray ionization quadrupole time-of-flight tandem mass spectrometry","volume":"8","author":"Pi","year":"2016","journal-title":"Anal. Methods"},{"issue":"7","key":"2026101511071421200_cit55","doi-asserted-by":"publisher","first-page":"1372","DOI":"10.1021\/jasms.3c00057","article-title":"Analysis of Phosphatidylinositol Modifications by Reactive Nitrogen Species Using LC-MS: Coming to Grips with Their Nitroxidative Susceptibility","volume":"34","author":"Bonciarelli","year":"2023","journal-title":"J. Am. Soc. Mass Spectrom."},{"issue":"11","key":"2026101511071421200_cit56","doi-asserted-by":"publisher","first-page":"986","DOI":"10.1016\/S1044-0305(00)00172-0","article-title":"Characterization of phosphatidylinositol, phosphatidylinositol-4-phosphate, and phosphatidylinositol-4,5-bisphosphate by electrospray ionization tandem mass spectrometry: a mechanistic study","volume":"11","author":"Hsu","year":"2000","journal-title":"J. Am. Soc. Mass Spectrom."},{"issue":"5","key":"2026101511071421200_cit57","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1016\/0009-2614(82)83229-6","article-title":"Bilinear rotation decoupling of homonuclear scalar interactions","volume":"93","author":"Garbow","year":"1982","journal-title":"Chem. Phys. Lett."},{"issue":"8","key":"2026101511071421200_cit58","doi-asserted-by":"publisher","first-page":"1178","DOI":"10.1021\/acs.biochem.6c00117","article-title":"Phosphatidylinositol Interactions with the SARS-CoV-2 Envelope Protein Investigated by Lipid 13C Labeling and Solid-State NMR","volume":"65","author":"Medeiros-Silva","year":"2026","journal-title":"Biochemistry"},{"issue":"3","key":"2026101511071421200_cit59","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1021\/cn2000926","article-title":"A\u03b2(1-42) Assembly in the Presence of scyllo-Inositol Derivatives: Identification of an Oxime Linkage as Important for the Development of Assembly Inhibitors","volume":"3","author":"Shaw","year":"2012","journal-title":"ACS Chem. Neurosci."},{"issue":"45","key":"2026101511071421200_cit60","doi-asserted-by":"publisher","first-page":"11968","DOI":"10.1073\/pnas.1702370114","article-title":"KO of 5-InsP7 kinase activity transforms the HCT116 colon cancer cell line into a hypermetabolic, growth-inhibited phenotype","volume":"114","author":"Gu","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"49","key":"2026101511071421200_cit61","doi-asserted-by":"publisher","first-page":"24551","DOI":"10.1073\/pnas.1911431116","article-title":"ITPK1 mediates the lipid-independent synthesis of inositol phosphates controlled by metabolism","volume":"116","author":"Desfoug\u00e8res","year":"2019","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"1","key":"2026101511071421200_cit62","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2019.05.015","article-title":"Phosphatidylinositol synthesis at the endoplasmic reticulum","volume":"1865","author":"Blunsom","year":"2020","journal-title":"Biochim. Biophys. Acta, Mol. Cell Biol. Lipids"},{"issue":"3","key":"2026101511071421200_cit63","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1038\/s41590-023-01419-y","article-title":"Phosphoinositide acyl chain saturation drives CD8+ effector T cell signaling and function","volume":"24","author":"Edwards-Hicks","year":"2023","journal-title":"Nat. Immunol."},{"issue":"8","key":"2026101511071421200_cit64","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4929","article-title":"Scyllo-inositol: Transverse relaxation time constant at 3 T and concentration changes associated with aging and alcohol use","volume":"36","author":"Deelchand","year":"2023","journal-title":"NMR Biomed."},{"key":"2026101511071421200_cit65","doi-asserted-by":"publisher","first-page":"607","DOI":"10.3390\/metabo15090607","article-title":"NMR-Based Metabolomic Profiling for Brain Cancer Diagnosis and Treatment Guidance","volume":"15","author":"Zickus","year":"2025","journal-title":"Metabolites"},{"key":"2026101511071421200_cit66","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2026.150167","article-title":"Multi-biofluid metabolomics coupled with gene network reveals stage-specific alterations in mild cognitive impairment and Alzheimer\u2019s disease in an ethnically mixed cohort","volume":"1874","author":"Cadaxo","year":"2026","journal-title":"Brain Res."},{"issue":"2","key":"2026101511071421200_cit67","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.pnmrs.2008.11.004","article-title":"High-resolution magic angle spinning NMR spectroscopy: Application to biomedical studies","volume":"55","author":"Lindon","year":"2009","journal-title":"Prog. Nucl. Magn. Reson. Spectrosc."}],"container-title":["ACS Chemical Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acschembio.6c00409","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/21\/9\/2232\/67418253\/cb-2026-00409e.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acbcct\/article-pdf\/21\/9\/2232\/67418253\/cb-2026-00409e.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T15:17:53Z","timestamp":1792077473000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acbcct\/article\/21\/9\/2232\/5329651\/Isotopic-Tracing-of-13C6-scyllo-Inositol-Uncovers"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,26]]},"references-count":67,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,8,26]]},"published-print":{"date-parts":[[2026,9,18]]}},"URL":"https:\/\/doi.org\/10.1021\/acschembio.6c00409","relation":{},"ISSN":["1554-8929","1554-8937"],"issn-type":[{"value":"1554-8929","type":"print"},{"value":"1554-8937","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,26]]}}}