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While nuclear magnetic resonance (NMR) spectroscopy is, in principle, ideally suited for such investigations, the absence of an amide proton precludes the use of conventional 1H\u201315N amide-detected experiments. 1H\u201313C-based approaches, though possible, remain challenging in large proteins. In this work, we present a selective labeling strategy that introduces an isolated 1H2\u03b4\u201313C\u03b4\u201315N spin system in proline residues in otherwise deuterated proteins. Our method leverages the enzymatic conversion of ornithine, synthesized from cost-effective isotopic precursors. We demonstrate the utility of this approach by applying it to a 134 kDa enzyme using magic-angle spinning NMR, highlighting its potential to efficiently probe functionally relevant sites in proteins.<\/jats:p>","DOI":"10.1021\/acschembio.6c00587","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:01:40Z","timestamp":1791838900000},"source":"Crossref","is-referenced-by-count":0,"title":["Chemoenzymatic Proline Isotope Labeling for Solution and Magic-Angle Spinning Protein Nuclear Magnetic Resonance"],"prefix":"10.1021","author":[{"given":"Darja I.","family":"Rohden","sequence":"first","affiliation":[{"name":"University of Vienna , , W\u00e4hringer Strasse 38 , ,","place":["Vienna, Austria, 1090"]},{"name":"Institute of Science and Technology Austria , Am Campus 1 , ,","place":["Klosterneuburg, Austria, 3400"]},{"name":"University of Vienna , , W\u00e4hringer Strasse 42 , ,","place":["Vienna, Austria, 1090"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9043-136X","authenticated-orcid":true,"given":"Federico","family":"Napoli","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology Austria , Am Campus 1 , ,","place":["Klosterneuburg, Austria, 3400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman J.","family":"Lichtenecker","sequence":"additional","affiliation":[{"name":"University of Vienna , , W\u00e4hringer Strasse 38 , ,","place":["Vienna, Austria, 1090"]},{"name":"MAG-LAB , Karl-Farkas Gasse 22 , ,","place":["Vienna, Austria, 1030"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9350-7606","authenticated-orcid":true,"given":"Paul","family":"Schanda","sequence":"additional","affiliation":[{"name":"Institute of Science and Technology Austria , Am Campus 1 , ,","place":["Klosterneuburg, Austria, 3400"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,13]]},"reference":[{"key":"2026101217013274400_cit1","doi-asserted-by":"publisher","first-page":"925","DOI":"10.1016\/S0022-2836(03)00759-9","article-title":"Stereospecific Interactions of Proline Residues in Protein Structures and Complexes","volume":"331","author":"Bhattacharyya","year":"2003","journal-title":"J. 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