{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T11:32:46Z","timestamp":1789817566987,"version":"4.0.1"},"reference-count":58,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2018,6,29]],"date-time":"2018-06-29T00:00:00Z","timestamp":1530230400000},"content-version":"vor","delay-in-days":17,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100010665","name":"H2020 Marie Sklodowska-Curie Actions","doi-asserted-by":"publisher","award":["642870"],"award-info":[{"award-number":["642870"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007136","name":"Secretar\u00eda de Estado de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["CTQ2014-58779-R"],"award-info":[{"award-number":["CTQ2014-58779-R"]}],"id":[{"id":"10.13039\/501100007136","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007136","name":"Secretar\u00eda de Estado de Investigaci\u00f3n, Desarrollo e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["CTQ2017-90039-R"],"award-info":[{"award-number":["CTQ2017-90039-R"]}],"id":[{"id":"10.13039\/501100007136","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,8,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Here, we describe a strategy for the rapid preparation of pure positional isomers of complex N-glycans to complement an existing array comprising a larger number of N-glycans and smaller glycan structures. The expanded array was then employed to study context-dependent binding of structural glycan fragments by monoclonal antibodies and C-type lectins. A partial enzymatic elongation of semiprotected core structures was combined with the protecting-group-aided separation of positional isomers by preparative HPLC. This methodology, which avoids the laborious chemical differentiation of antennae, was employed for the preparation of eight biantennary N-glycans with Gal\u03b21,4GlcNAc (LN), GalNAc\u03b21,4GlcNAc (LDN), and GalNAc\u03b21,4[Fuc\u03b11,3]GlcNAc (LDNF) motifs presented on either one or both antennae. Screening of the binding specificities of three anti-LeX monoclonal IgM antibodies raised against S. mansoni glycans and three C-type lectin receptors of the innate immune system, namely DC-SIGN, DC-SIGNR, and LSECtin, revealed a surprising context-dependent fine specificity for the recognition of the glycan motifs. Moreover, we observed a striking selection of one individual positional isomer over the other by the C-type lectins tested, underscoring the biological relevance of the structural context of glycan elements in molecular recognition.<\/jats:p>","DOI":"10.1021\/acschembio.8b00431","type":"journal-article","created":{"date-parts":[[2018,6,12]],"date-time":"2018-06-12T13:19:19Z","timestamp":1528809559000},"page":"2269-2279","source":"Crossref","is-referenced-by-count":49,"title":["Chemoenzymatic Synthesis of N-glycan Positional\nIsomers and Evidence for Branch Selective Binding by Monoclonal Antibodies\nand Human C-type Lectin Receptors"],"prefix":"10.1021","volume":"13","author":[{"given":"Begon\u0303a","family":"Echeverria","sequence":"first","affiliation":[{"name":"CIC biomaGUNE, Glycotechnology Laboratory , Paseo Miramo\u0301n 182 , ,","place":["San Sebastian, Spain, 20014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonia","family":"Serna","sequence":"additional","affiliation":[{"name":"CIC biomaGUNE, Glycotechnology Laboratory , Paseo Miramo\u0301n 182 , ,","place":["San Sebastian, Spain, 20014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Achilli","sequence":"additional","affiliation":[{"name":"Universite\u0301 Grenoble Alpes, CEA, CNRS, IBS , ,","place":["Grenoble, France, F-38000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Vive\u0300s","sequence":"additional","affiliation":[{"name":"Universite\u0301 Grenoble Alpes, CEA, CNRS, IBS , ,","place":["Grenoble, France, F-38000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Pham","sequence":"additional","affiliation":[{"name":"CIC biomaGUNE, Glycotechnology Laboratory , Paseo Miramo\u0301n 182 , ,","place":["San Sebastian, Spain, 20014"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"The\u0301paut","sequence":"additional","affiliation":[{"name":"Universite\u0301 Grenoble Alpes, CEA, CNRS, IBS , ,","place":["Grenoble, France, F-38000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelis H.","family":"Hokke","sequence":"additional","affiliation":[{"name":"Leiden University Medical Center , , ,","place":["Leiden, The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"Fieschi","sequence":"additional","affiliation":[{"name":"Universite\u0301 Grenoble Alpes, CEA, CNRS, IBS , ,","place":["Grenoble, France, F-38000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9092-7023","authenticated-orcid":true,"given":"Niels-Christian","family":"Reichardt","sequence":"additional","affiliation":[{"name":"CIC biomaGUNE, Glycotechnology Laboratory , Paseo Miramo\u0301n 182 , ,","place":["San Sebastian, Spain, 20014"]},{"name":"CIBER-BBN , Paseo Miramo\u0301n 182 , ,","place":["San Sebastian, Spain, 20014"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2018,6,12]]},"reference":[{"key":"2026090120220607300_cit1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1093\/glycob\/cww086","article-title":"Biological\nroles of glycans","volume":"27","author":"Varki","year":"2017","journal-title":"Glycobiology"},{"key":"2026090120220607300_cit2","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1038\/ni.f.203","article-title":"Protein-glycan\ninteractions in the control of innate and adaptive immune responses","volume":"9","author":"Van Kooyk","year":"2008","journal-title":"Nat. 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