{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T14:32:19Z","timestamp":1793457139251,"version":"4.5.0"},"reference-count":51,"publisher":"American Chemical Society (ACS)","issue":"11","license":[{"start":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T00:00:00Z","timestamp":1793404800000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFC2102100"],"award-info":[{"award-number":["2021YFC2102100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2307703"],"award-info":[{"award-number":["2023YFC2307703"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7252170"],"award-info":[{"award-number":["7252170"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["92478116"],"award-info":[{"award-number":["92478116"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","award":["20220484072"],"award-info":[{"award-number":["20220484072"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","award":["20240484724"],"award-info":[{"award-number":["20240484724"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Brucellosis, a global zoonosis caused by Brucella species, currently lacks safe vaccines for human use, while existing veterinary live-attenuated vaccines pose infection risks. Although ferritin nanoparticle materials have shown significant advantages in delivery, there is not much research on loading complex polysaccharide antigens. Here, we engineered a bioconjugate nanovaccine (Fn-OPS) through a bacterial glycosylation-driven synthetic strategy, which couples Yersinia enterocolitica O:9 (YeO9) O-specific polysaccharide (OPS)\u2500a structural analogue of Brucella antigens\u2500to a self-assembled ferritin nanoparticle (Fn). This nanocarrier platform combines the symmetrical architecture and antigen-presenting advantages of Fn with the precision of enzymatic glycosylation, addressing the limitations of complex chemical synthesis methods. After confirming the presence of monodisperse nanoparticles with stability at room temperature and great in vivo safety, we performed murine immunization studies and demonstrated a robust activation of T follicular helper (Tfh) cells and germinal center B cells, leading to the production of high-titer IgG antibodies that are cross-reactive with Brucella lipopolysaccharide. This immune response provides strong protection against Brucella infection. This work establishes a ferritin-based nanoconjugate platform for targeted delivery of complex polysaccharide antigens. It advances a scalable, biosafe strategy for Brucella vaccines, expanding the toolkit for targeted antigen delivery in conjugate vaccine design and broadening applications in infectious disease prophylaxis.<\/jats:p>","DOI":"10.1021\/acsinfecdis.5c00260","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T15:08:37Z","timestamp":1761923317000},"page":"3050-3060","source":"Crossref","is-referenced-by-count":2,"title":["Development\nof a Promising Ferritin-Based Bioconjugate\nNanovaccine Candidate against Brucellosis"],"prefix":"10.1021","volume":"11","author":[{"given":"Wenjun","family":"Li","sequence":"first","affiliation":[{"name":"The Third Medical Centre of Chinese PLA General Hospital, The Training Site for Postgraduate of Jin Zhou Medical University , , ,","place":["Beijing, China, 100039"]},{"name":"Beijing Institute of Biotechnology , , ,","place":["Beijing, China, 100071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juntao","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology , , ,","place":["Beijing, China, 100071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueping","family":"Cao","sequence":"additional","affiliation":[{"name":"The Third Medical Centre of Chinese PLA General Hospital, The Training Site for Postgraduate of Jin Zhou Medical University , , ,","place":["Beijing, China, 100039"]},{"name":"The Third Medical Center, PLA General Hospital , ,","place":["Beijing, China, 100039"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Song","sequence":"additional","affiliation":[{"name":"The Third Medical Center, PLA General Hospital , ,","place":["Beijing, China, 100039"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology , , ,","place":["Beijing, China, 100071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingqin","family":"Ye","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology , , ,","place":["Beijing, China, 100071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology , , ,","place":["Beijing, China, 100071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Lyu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology , , ,","place":["Beijing, China, 100071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9975-8978","authenticated-orcid":true,"given":"Li","family":"Zhu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology , , ,","place":["Beijing, China, 100071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyang","family":"Liu","sequence":"additional","affiliation":[{"name":"The Third Medical Center, PLA General Hospital , ,","place":["Beijing, China, 100039"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Yang","sequence":"additional","affiliation":[{"name":"The Third Medical Centre of Chinese PLA General Hospital, The Training Site for Postgraduate of Jin Zhou Medical University , , ,","place":["Beijing, China, 100039"]},{"name":"The Third Medical Center, PLA General Hospital , ,","place":["Beijing, China, 100039"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengliang","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology , , ,","place":["Beijing, China, 100071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5286-0614","authenticated-orcid":true,"given":"Chao","family":"Pan","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology , , ,","place":["Beijing, China, 100071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Wang","sequence":"additional","affiliation":[{"name":"The Third Medical Centre of Chinese PLA General Hospital, The Training Site for Postgraduate of Jin Zhou Medical University , , ,","place":["Beijing, China, 100039"]},{"name":"The Third Medical Center, PLA General Hospital , ,","place":["Beijing, China, 100039"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,10,31]]},"reference":[{"issue":"1","key":"2026103110061302800_cit1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2023.2280377","article-title":"One-step preparation\nof a self-assembled bioconjugate nanovaccine against Brucella","volume":"14","author":"Huang","year":"2023","journal-title":"Virulence"},{"issue":"1","key":"2026103110061302800_cit2","doi-asserted-by":"publisher","DOI":"10.1080\/20008686.2018.1500846","article-title":"Comparisons of brucellosis between\nhuman and veterinary\nmedicine","volume":"8","author":"Hull","year":"2018","journal-title":"Infect. Ecol. Epidemiol."},{"issue":"12","key":"2026103110061302800_cit3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040317","article-title":"Perspectives\nfor the Treatment of Brucellosis in the\n21st Century: The Ioannina Recommendations","volume":"4","author":"Ariza","year":"2007","journal-title":"PLoS Med."},{"key":"2026103110061302800_cit4","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2013\/743509","article-title":"A History\nof the Development ofBrucellaVaccines","volume":"2013","author":"Avila-Caldero\u0301n","year":"2013","journal-title":"BioMed.\nRes. Int."},{"issue":"1","key":"2026103110061302800_cit5","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1186\/s13567-015-0199-7","article-title":"Recent advances\nin Brucella abortus vaccines","volume":"46","author":"Dorneles","year":"2015","journal-title":"Vet. Res."},{"issue":"456","key":"2026103110061302800_cit6","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aat4615","article-title":"Glycoconjugate\nvaccines: Principles and mechanisms","volume":"10","author":"Rappuoli","year":"2018","journal-title":"Sci. Transl.\nMed."},{"issue":"12","key":"2026103110061302800_cit7","doi-asserted-by":"publisher","first-page":"1551","DOI":"10.1038\/nm.2587","article-title":"A sweet T cell response","volume":"17","author":"Rappuoli","year":"2011","journal-title":"Nature\nMedicine"},{"issue":"1","key":"2026103110061302800_cit8","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1038\/s41541-019-0110-z","article-title":"Recent advances in the production\nof recombinant glycoconjugate vaccines","volume":"4","author":"Kay","year":"2019","journal-title":"npj\nVaccines"},{"issue":"7","key":"2026103110061302800_cit9","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1099\/jmm.0.039438-0","article-title":"Recent developments\nin bacterial\nprotein glycan coupling technology and glycoconjugate vaccine design","volume":"61","author":"Terra","year":"2012","journal-title":"J. Med. Microbiol"},{"issue":"7","key":"2026103110061302800_cit10","doi-asserted-by":"publisher","first-page":"703","DOI":"10.3390\/vaccines13070703","article-title":"Recent\nAdvances in Bioconjugate Vaccine\nDevelopment","volume":"13","author":"Wren","year":"2025","journal-title":"Vaccines"},{"issue":"5599","key":"2026103110061302800_cit11","doi-asserted-by":"publisher","first-page":"1790","DOI":"10.1126\/science.298.5599.1790","article-title":"N-Linked Glycosylation\nin Campylobacter jejuni and Its Functional Transfer into E. coli","volume":"298","author":"Wacker","year":"2002","journal-title":"Science"},{"issue":"15","key":"2026103110061302800_cit12","doi-asserted-by":"publisher","first-page":"10578","DOI":"10.1074\/jbc.M112.432815","article-title":"In Vitro\nActivity of Neisseria meningitidis PglL O-Oligosaccharyltransferase\nwith Diverse Synthetic Lipid Donors and a UDP-activated Sugar","volume":"288","author":"Musumeci","year":"2013","journal-title":"J. Biol. Chem."},{"issue":"1","key":"2026103110061302800_cit13","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1038\/s41541-017-0037-1","article-title":"Design and production\nof conjugate vaccines against S. Paratyphi\nA using an O-linked glycosylation system in vivo","volume":"3","author":"Sun","year":"2018","journal-title":"npj Vaccines"},{"issue":"42","key":"2026103110061302800_cit14","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202002940","article-title":"Biosynthesis of Self-Assembled Proteinaceous Nanoparticles\nfor Vaccination","volume":"32","author":"Pan","year":"2020","journal-title":"Adv. Mater."},{"issue":"4","key":"2026103110061302800_cit15","doi-asserted-by":"publisher","first-page":"347","DOI":"10.3390\/vaccines12040347","article-title":"Production\nof Promising\nHeat-Labile Enterotoxin (LT) B Subunit-Based Self-Assembled Bioconjugate\nNanovaccines against Infectious Diseases","volume":"12","author":"Li","year":"2024","journal-title":"Vaccines"},{"issue":"8","key":"2026103110061302800_cit16","doi-asserted-by":"publisher","first-page":"728","DOI":"10.3390\/nano14080728","article-title":"Efficient\nProduction of Self-Assembled Bioconjugate Nanovaccines against Klebsiella\npneumoniae O2 Serotype in Engineered Escherichia coli","volume":"14","author":"Zhang","year":"2024","journal-title":"Nanomaterials"},{"issue":"20","key":"2026103110061302800_cit17","doi-asserted-by":"publisher","first-page":"7244","DOI":"10.1128\/JB.00605-07","article-title":"Characterization\nand Biological Role of the O-Polysaccharide Gene Cluster ofYersinia\nenterocoliticaSerotype O:9","volume":"189","author":"Skurnik","year":"2007","journal-title":"J. Bacteriol."},{"issue":"3","key":"2026103110061302800_cit18","doi-asserted-by":"publisher","first-page":"436","DOI":"10.3390\/microorganisms8030436","article-title":"Application of an O-Linked Glycosylation System in Yersinia enterocolitica\nSerotype O:9 to Generate a New Candidate Vaccine against Brucella\nabortus","volume":"8","author":"Huang","year":"2020","journal-title":"Microorganisms"},{"issue":"1","key":"2026103110061302800_cit19","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1186\/1475-2859-11-13","article-title":"Exploiting the Campylobacter\njejuni protein glycosylation\nsystem for glycoengineering vaccines and diagnostic tools directed\nagainst brucellosis","volume":"11","author":"Iwashkiw","year":"2012","journal-title":"Microb. Cell Fact."},{"issue":"8","key":"2026103110061302800_cit20","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1038\/nri3488","article-title":"Applications of\nnanotechnology for immunology","volume":"13","author":"Smith","year":"2013","journal-title":"Nat. Rev. Immunol"},{"key":"2026103110061302800_cit21","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2021.113867","article-title":"Prophylactic vaccine\ndelivery systems against epidemic infectious diseases","volume":"176","author":"Pan","year":"2021","journal-title":"Adv. Drug Delivery Rev."},{"issue":"1","key":"2026103110061302800_cit22","doi-asserted-by":"publisher","DOI":"10.1002\/EXP.20210117","article-title":"Recent advances\nin targeted antibacterial therapy basing\non nanomaterials","volume":"3","author":"Geng","year":"2023","journal-title":"Exploration"},{"issue":"2","key":"2026103110061302800_cit23","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28503","article-title":"A hepatitis\nB virus core antigen-based virus-like particle vaccine expressing\nSARS-CoV-2 B and T cell epitopes induces epitope-specific humoral\nand cell-mediated immune responses but confers limited protection\nagainst SARS-CoV-2 infection","volume":"95","author":"Hassebroek","year":"2023","journal-title":"J. Med. Virol."},{"key":"2026103110061302800_cit24","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2024.122946","article-title":"Rapid and efficient\nimmune response induced by a designed modular cholera toxin B subunit\n(CTB)-based self-assembling nanoparticle","volume":"315","author":"Pan","year":"2025","journal-title":"Biomaterials"},{"issue":"4","key":"2026103110061302800_cit25","doi-asserted-by":"publisher","first-page":"403","DOI":"10.3390\/vaccines9040403","article-title":"AP205 VLPs\nBased on Dimerized Capsid\nProteins Accommodate RBM Domain of SARS-CoV-2 and Serve as an Attractive\nVaccine Candidate","volume":"9","author":"Liu","year":"2021","journal-title":"Vaccines"},{"issue":"8","key":"2026103110061302800_cit26","doi-asserted-by":"publisher","first-page":"846","DOI":"10.1016\/j.bbagen.2010.03.005","article-title":"Ferritin in the field of nanodevices","volume":"1800","author":"Yamashita","year":"2010","journal-title":"Biochim. Biophys. Acta, Gen. Subj"},{"issue":"1","key":"2026103110061302800_cit27","doi-asserted-by":"publisher","first-page":"2149","DOI":"10.1038\/s41467-023-37417-9","article-title":"A ferritin-based COVID-19 nanoparticle vaccine that\nelicits robust, durable, broad-spectrum neutralizing antisera in non-human\nprimates","volume":"14","author":"Weidenbacher","year":"2023","journal-title":"Nat. Commun."},{"issue":"2","key":"2026103110061302800_cit28","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1038\/s41591-021-01660-8","article-title":"Safety and\nimmunogenicity of a ferritin nanoparticle H2 influenza vaccine in\nhealthy adults: a phase 1 trial","volume":"28","author":"Houser","year":"2022","journal-title":"Nat. Med."},{"issue":"5","key":"2026103110061302800_cit29","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1038\/s41565-020-0648-y","article-title":"Dual-targeting nanoparticle vaccine\nelicits a therapeutic antibody response against chronic hepatitis\nB","volume":"15","author":"Wang","year":"2020","journal-title":"Nat. Nanotechnol"},{"issue":"10","key":"2026103110061302800_cit30","doi-asserted-by":"publisher","first-page":"1621","DOI":"10.3390\/pharmaceutics13101621","article-title":"Functionalizing\nFerritin Nanoparticles\nfor Vaccine Development","volume":"13","author":"Rodrigues","year":"2021","journal-title":"Pharmaceutics"},{"issue":"2","key":"2026103110061302800_cit31","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00443-16","article-title":"Biosynthesis of Conjugate\nVaccines Using an O-Linked Glycosylation System","volume":"7","author":"Pan","year":"2016","journal-title":"mBio"},{"key":"2026103110061302800_cit32","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1125503","article-title":"The transcriptional program during\ngerminal center reaction - a close view at GC B cells, Tfh cells and\nTfr cells","volume":"14","author":"Betzler","year":"2023","journal-title":"Front. Immunol."},{"issue":"1","key":"2026103110061302800_cit33","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1146\/annurev-immunol-120419-022408","article-title":"Germinal Centers","volume":"40","author":"Victora","year":"2022","journal-title":"Annu. Rev.\nImmunol."},{"issue":"1","key":"2026103110061302800_cit34","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1080\/1040841X.2018.1547681","article-title":"Protein glycosylation:\nSweet or bitter for bacterial pathogens?","volume":"45","author":"Bhat","year":"2019","journal-title":"Crit.\nRev. Microbiol"},{"issue":"6","key":"2026103110061302800_cit35","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1080\/14760584.2020.1775077","article-title":"Improving\nprotein\nglycan coupling technology (PGCT) for glycoconjugate vaccine production","volume":"19","author":"Dow","year":"2020","journal-title":"Expert Rev. Vaccines"},{"issue":"1","key":"2026103110061302800_cit36","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1186\/1471-2180-9-92","article-title":"DNA polymorphism analysis of Brucella\nlipopolysaccharide\ngenes reveals marked differences in O-polysaccharide biosynthetic\ngenes between smooth and rough Brucella species and novel species-specific\nmarkers","volume":"9","author":"Zygmunt","year":"2009","journal-title":"BMC Microbiol."},{"issue":"12","key":"2026103110061302800_cit37","doi-asserted-by":"publisher","first-page":"4392","DOI":"10.1128\/IAI.00856-13","article-title":"Comparison of Cytokine Immune Responses\nto Brucella abortus and Yersinia enterocolitica Serotype O:9 Infections\nin BALB\/c Mice","volume":"81","author":"Gu","year":"2013","journal-title":"Infect. Immun."},{"issue":"3","key":"2026103110061302800_cit38","doi-asserted-by":"publisher","first-page":"546","DOI":"10.3390\/vaccines11030546","article-title":"Safe Subunit Green Vaccines Confer\nRobust Immunity\nand Protection against Mucosal Brucella Infection in Mice","volume":"11","author":"Abushahba","year":"2023","journal-title":"Vaccines"},{"issue":"1","key":"2026103110061302800_cit39","doi-asserted-by":"publisher","first-page":"30","DOI":"10.3390\/vaccines12010030","article-title":"Nanoparticles\nand Antiviral Vaccines","volume":"12","author":"Liu","year":"2023","journal-title":"Vaccines"},{"issue":"1","key":"2026103110061302800_cit40","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1146\/annurev-chembioeng-060718-030347","article-title":"Protein and Peptide Biomaterials\nfor Engineered Subunit\nVaccines and Immunotherapeutic Applications","volume":"10","author":"Tsoras","year":"2019","journal-title":"Annu. Rev. Chem. Biomol. Eng."},{"issue":"10","key":"2026103110061302800_cit41","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.1080\/14760584.2021.1984890","article-title":"Nanovaccine: an emerging\nstrategy","volume":"20","author":"Das","year":"2021","journal-title":"Expert Rev.\nVaccines"},{"issue":"1","key":"2026103110061302800_cit42","doi-asserted-by":"publisher","first-page":"6","DOI":"10.2174\/2211738507666191024162042","article-title":"Nanoparticles and Vaccine Development","volume":"8","author":"Kheirollahpour","year":"2020","journal-title":"Pharm. Nanotechnol"},{"key":"2026103110061302800_cit43","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.clim.2017.08.004","article-title":"Adjuvant formulations\nfor virus-like particle (VLP)\nbased vaccines","volume":"183","author":"Cimica","year":"2017","journal-title":"Clin. Immunol"},{"issue":"11","key":"2026103110061302800_cit44","doi-asserted-by":"publisher","first-page":"16982","DOI":"10.1021\/acsnano.1c04996","article-title":"Lipid Nanoparticles\u2500From\nLiposomes to mRNA Vaccine Delivery, a Landscape of Research Diversity\nand Advancement","volume":"15","author":"Tenchov","year":"2021","journal-title":"ACS Nano"},{"key":"2026103110061302800_cit45","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2022.114416","article-title":"The role of lipid\ncomponents in lipid nanoparticles for vaccines and gene therapy","volume":"188","author":"Hald Albertsen","year":"2022","journal-title":"Adv. Drug Deliv Rev."},{"issue":"17","key":"2026103110061302800_cit46","doi-asserted-by":"publisher","first-page":"3313","DOI":"10.1021\/acs.accounts.1c00267","article-title":"Ferritin: A Multifunctional Nanoplatform\nfor Biological Detection,\nImaging Diagnosis, and Drug Delivery","volume":"54","author":"Song","year":"2021","journal-title":"Acc. Chem.\nRes."},{"issue":"17","key":"2026103110061302800_cit47","doi-asserted-by":"publisher","first-page":"4221","DOI":"10.3390\/molecules29174221","article-title":"Self-Assembled\nFerritin\nNanoparticles for Delivery of Antigens and Development of Vaccines:\nFrom Structure and Property to Applications","volume":"29","author":"Cao","year":"2024","journal-title":"Molecules"},{"issue":"7862","key":"2026103110061302800_cit48","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1038\/s41586-021-03530-2","article-title":"Adjuvanting\na subunit COVID-19 vaccine to induce protective immunity","volume":"594","author":"Arunachalam","year":"2021","journal-title":"Nature"},{"issue":"6","key":"2026103110061302800_cit49","doi-asserted-by":"publisher","first-page":"3013","DOI":"10.1021\/acs.chemrev.3c00409","article-title":"The Promise\nand Potential of Metal-Organic Frameworks and Covalent Organic Frameworks\nin Vaccine Nanotechnology","volume":"124","author":"Wijesundara","year":"2024","journal-title":"Chem. Rev."},{"key":"2026103110061302800_cit50","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1121074","article-title":"A bioconjugate\nvaccine against Brucella abortus produced by engineered\nEscherichia coli","volume":"11","author":"Li","year":"2023","journal-title":"Front. Bioeng. Biotech"},{"key":"2026103110061302800_cit51","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2022.146521","article-title":"The Genome\nSequence of Brucella abortus vaccine strain A19 provides insights\non its virulence attenuation compared to Brucella abortus strain 9\u2013941","volume":"830","author":"Wang","year":"2022","journal-title":"Gene"}],"container-title":["ACS Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsinfecdis.5c00260","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/11\/11\/3050\/69042534\/acsinfecdis.5c00260.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/11\/11\/3050\/69042534\/acsinfecdis.5c00260.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T14:23:59Z","timestamp":1793456639000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article\/11\/11\/3050\/3663112\/Development-of-a-Promising-Ferritin-Based"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,31]]},"references-count":51,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2025,10,31]]},"published-print":{"date-parts":[[2025,11,14]]}},"URL":"https:\/\/doi.org\/10.1021\/acsinfecdis.5c00260","relation":{},"ISSN":["2373-8227"],"issn-type":[{"value":"2373-8227","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,31]]}}}