{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T17:35:04Z","timestamp":1794332104830,"version":"4.9.0"},"reference-count":77,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000106","name":"Office of Integrative Activities","doi-asserted-by":"publisher","award":["2521342"],"award-info":[{"award-number":["2521342"]}],"id":[{"id":"10.13039\/100000106","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000152","name":"Division of Molecular and Cellular Biosciences","doi-asserted-by":"publisher","award":["1922542"],"award-info":[{"award-number":["1922542"]}],"id":[{"id":"10.13039\/100000152","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000152","name":"Division of Molecular and Cellular Biosciences","doi-asserted-by":"publisher","award":["2240028"],"award-info":[{"award-number":["2240028"]}],"id":[{"id":"10.13039\/100000152","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["SD00H763 22"],"award-info":[{"award-number":["SD00H763 22"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010221","name":"Higher Education Commision, Pakistan","doi-asserted-by":"publisher","award":["Ref No. 1-2\/HRD\/US\/2022\/474"],"award-info":[{"award-number":["Ref No. 1-2\/HRD\/US\/2022\/474"]}],"id":[{"id":"10.13039\/501100010221","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,12,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The global rise of antibiotic-resistant pathogens has created an imperative to discover novel antimicrobial strategies. Traditional antibiotics predominantly target essential bacterial processes, such as cell division, DNA replication, transcription, and translation, but few new agents targeting these pathways have emerged in recent decades. We explored an alternative approach by identifying small molecules that hyperactivate the bacterial ClpP protease, thereby inducing uncontrolled proteolysis and ultimately leading to bacterial cell death. Leveraging the known binding interactions of the peptide antibiotic ADEP4 with ClpP, we performed high-throughput in silico screening. Molecular docking simulations prioritized compounds based on predicted binding affinity (kcal\/mol), complemented by structural chemistry evaluation and in silico pharmacokinetic profiling. AGI-6780 emerged as a lead compound with high predicted affinity for the ClpP active site. In vitro assays showed that AGI-6780 effectively inhibits a panel of Gram-positive bacteria by targeting ClpP. It also exhibits synergy with the antibiotic rifampicin and has minimal cytotoxicity on human cell lines. AGI-6780 is a promising antimicrobial agent that uniquely exploits ClpP, an unconventional bacterial target.<\/jats:p>","DOI":"10.1021\/acsinfecdis.5c00666","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T16:33:38Z","timestamp":1762792418000},"page":"3520-3528","source":"Crossref","is-referenced-by-count":2,"title":["Repurposing\nAGI-6780 to Activate ClpP and Target Gram-Positive\nBacteria"],"prefix":"10.1021","volume":"11","author":[{"given":"Roha","family":"Razzaq","sequence":"first","affiliation":[{"name":"South Dakota State University , , , ,","place":["Brookings, South Dakota, United States, 57006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazera Khatun","family":"Koly","sequence":"additional","affiliation":[{"name":"South Dakota State University , , , ,","place":["Brookings, South Dakota, United States, 57006"]},{"name":"South Dakota State University , , , ,","place":["Brookings, South Dakota, United States, 57006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahmina","family":"Hossain","sequence":"additional","affiliation":[{"name":"South Dakota State University , , , ,","place":["Brookings, South Dakota, United States, 57006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3807-8666","authenticated-orcid":true,"given":"Nicholas C.","family":"Butzin","sequence":"additional","affiliation":[{"name":"South Dakota State University , , , ,","place":["Brookings, South Dakota, United States, 57006"]},{"name":"South Dakota State University , , , ,","place":["Brookings, South Dakota, United States, 57006"]},{"name":"South Dakota State University , , , ,","place":["Brookings, South Dakota, United States, 57007"]},{"name":"South Dakota Mines , , ,","place":["Rapid City, South Dakota, United States, 57701"]},{"name":"University of Nevada , , ,","place":["Las Vegas, Nevada, United States, 89154"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,11,10]]},"reference":[{"issue":"11","key":"2026111011123578400_cit1","doi-asserted-by":"publisher","first-page":"1637","DOI":"10.3390\/antibiotics12111637","article-title":"Genomic Diversity, Antimicrobial\nResistance, Plasmidome, and Virulence Profiles of Salmonella Isolated\nfrom Small Specialty Crop Farms Revealed by Whole-Genome Sequencing","volume":"12","author":"Bhandari","year":"2023","journal-title":"Antibiotics (Basel)"},{"issue":"7","key":"2026111011123578400_cit2","doi-asserted-by":"publisher","first-page":"1468","DOI":"10.3390\/microorganisms9071468","article-title":"Analysis of 56,348 Genomes Identifies\nthe Relationship\nbetween Antibiotic and Metal Resistance and the Spread of Multidrug-Resistant\nNon-Typhoidal Salmonella","volume":"9","author":"Fenske","year":"2021","journal-title":"Microorganisms"},{"issue":"5759","key":"2026111011123578400_cit3","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1126\/science.1120800","article-title":"Sampling the Antibiotic\nResistome","volume":"311","author":"D\u2019Costa","year":"2006","journal-title":"Science"},{"issue":"3","key":"2026111011123578400_cit4","doi-asserted-by":"publisher","first-page":"19","DOI":"10.3390\/medicina47030019","article-title":"Antibiotic\nResistance Mechanisms of Clinically Important Bacteria","volume":"47","author":"Giedraitiene\u0307","year":"2011","journal-title":"Medicina"},{"issue":"6\u20137","key":"2026111011123578400_cit5","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.ijmm.2013.02.009","article-title":"Intrinsic antibiotic resistance: mechanisms, origins,\nchallenges and solutions","volume":"303","author":"Cox","year":"2013","journal-title":"International Journal\nof Medical Microbiology"},{"key":"2026111011123578400_cit6","doi-asserted-by":"crossref","DOI":"10.15620\/cdc:82532","volume-title":"Antibiotic\nResistance Threats in the United\nStates, 2019","author":"Centers for Disease, C.; Prevention.","year":"2019"},{"issue":"10325","key":"2026111011123578400_cit7","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial\nantimicrobial resistance\nin 2019: a systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"Lancet"},{"issue":"12","key":"2026111011123578400_cit8","doi-asserted-by":"publisher","DOI":"10.15252\/embr.202051034","article-title":"Antibiotic\nresistance and persistence\u2500Implications for human health and\ntreatment perspectives","volume":"21","author":"Huemer","year":"2020","journal-title":"EMBO Report"},{"key":"2026111011123578400_cit9","first-page":"1","volume-title":"Global Cancer Facts &\nFigures Atlanta","author":"Society, A. C. American Cancer Society.","year":"2018","edition":"4"},{"issue":"6","key":"2026111011123578400_cit10","doi-asserted-by":"publisher","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and\nmortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. Clin"},{"issue":"4","key":"2026111011123578400_cit11","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1038\/ja.2016.138","article-title":"Bacterial proteases,\nuntapped antimicrobial drug targets","volume":"70","author":"Culp","year":"2017","journal-title":"J. Antibiot."},{"issue":"7","key":"2026111011123578400_cit12","doi-asserted-by":"publisher","first-page":"1385","DOI":"10.3390\/microorganisms10071385","article-title":"Bacterial stress responses\nas potential targets in overcoming antibiotic\nresistance","volume":"10","author":"Dawan","year":"2022","journal-title":"Microorganisms"},{"issue":"9","key":"2026111011123578400_cit13","doi-asserted-by":"publisher","first-page":"2232","DOI":"10.3390\/ijms20092232","article-title":"ClpP Protease,\na Promising Antimicrobial\nTarget","volume":"20","author":"Moreno-Cinos","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"2026111011123578400_cit14","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1146\/annurev-physiol-021317-121303","article-title":"Mechanical\nProtein Unfolding and\nDegradation","volume":"80","author":"Olivares","year":"2018","journal-title":"Annu. Rev. Physiol."},{"issue":"4","key":"2026111011123578400_cit15","doi-asserted-by":"publisher","first-page":"1193","DOI":"10.1021\/acs.jnatprod.5b01091","article-title":"Sclerotiamide:\nthe first non-peptide-based natural product activator of bacterial\ncaseinolytic protease P","volume":"79","author":"Lavey","year":"2016","journal-title":"J. Nat. Prod."},{"issue":"7","key":"2026111011123578400_cit16","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1039\/C6NP00125D","article-title":"Conformational\ncontrol of the bacterial\nClp protease by natural product antibiotics","volume":"34","author":"Malik","year":"2017","journal-title":"Nat. Prod. Report"},{"issue":"10","key":"2026111011123578400_cit17","doi-asserted-by":"publisher","first-page":"1082","DOI":"10.1038\/nm1306","article-title":"Dysregulation of bacterial proteolytic machinery by\na new class of antibiotics","volume":"11","author":"Brotz-Oesterhelt","year":"2005","journal-title":"Nat. Med."},{"issue":"11","key":"2026111011123578400_cit18","doi-asserted-by":"publisher","first-page":"1093","DOI":"10.1021\/np960607m","article-title":"Sclerotiamide: a\nnew member of the paraherquamide class with potent antiinsectan activity\nfrom the sclerotia of Aspergillus sclerotiorum","volume":"59","author":"Whyte","year":"1996","journal-title":"J. Nat. Prod."},{"key":"2026111011123578400_cit19","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1038\/s42003-019-0656-3","article-title":"ClpP protease activation\nresults from the reorganization of the electrostatic interaction networks\nat the entrance pores","volume":"2","author":"Mabanglo","year":"2019","journal-title":"Commun. Biol."},{"issue":"8","key":"2026111011123578400_cit20","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00424-18","article-title":"In Vivo and In Vitro Effects of a\nClpP-Activating Antibiotic against Vancomycin-Resistant Enterococci","volume":"62","author":"Brown Gandt","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"issue":"7476","key":"2026111011123578400_cit21","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1038\/nature12790","article-title":"Activated ClpP kills\npersisters and eradicates a chronic biofilm\ninfection","volume":"503","author":"Conlon","year":"2013","journal-title":"Nature"},{"issue":"1","key":"2026111011123578400_cit22","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1002\/phar.1868","article-title":"Past, present,\nand future of antibacterial\neconomics: increasing bacterial resistance, limited antibiotic pipeline,\nand societal implications","volume":"37","author":"Luepke","year":"2017","journal-title":"Pharmacotherapy"},{"issue":"4","key":"2026111011123578400_cit23","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1038\/s41564-019-0357-1","article-title":"Drug repurposing\nfor antimicrobial discovery","volume":"4","author":"Farha","year":"2019","journal-title":"Nat. Microbiol."},{"issue":"12","key":"2026111011123578400_cit24","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.3390\/pharmaceutics12121189","article-title":"The delivery of\nthe novel drug \u2018halicin\u2019using electrospun fibers for\nthe treatment of pressure ulcer against pathogenic bacteria","volume":"12","author":"Aburayan","year":"2020","journal-title":"Pharmaceutics"},{"issue":"12","key":"2026111011123578400_cit25","doi-asserted-by":"publisher","first-page":"1480","DOI":"10.3390\/antibiotics10121480","article-title":"Assessment\nof the antibacterial efficacy of halicin against pathogenic bacteria","volume":"10","author":"Booq","year":"2021","journal-title":"Antibiotics"},{"issue":"6","key":"2026111011123578400_cit26","doi-asserted-by":"publisher","first-page":"492","DOI":"10.3390\/antibiotics13060492","article-title":"Halicin: A New Horizon\nin Antibacterial Therapy against Veterinary Pathogens","volume":"13","author":"Wang","year":"2024","journal-title":"Antibiotics"},{"key":"2026111011123578400_cit27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1002\/9781394159338.ch1","article-title":"Quantum\nComputational Concepts and\nApproaches in Drug Discovery","author":"Meenakshi","year":"2024","journal-title":"Dev. Delivery."},{"issue":"7","key":"2026111011123578400_cit28","doi-asserted-by":"publisher","first-page":"4264","DOI":"10.1128\/AAC.00535-16","article-title":"A Pathogen-Selective Antibiotic Minimizes Disturbance\nto the Microbiome","volume":"60","author":"Yao","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"issue":"2","key":"2026111011123578400_cit29","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1002\/jcc.21334","article-title":"AutoDock Vina: improving the speed and accuracy of\ndocking with a new scoring function, efficient optimization, and multithreading","volume":"31","author":"Trott","year":"2010","journal-title":"Effic. Optim. Multithreading"},{"issue":"14","key":"2026111011123578400_cit30","doi-asserted-by":"publisher","first-page":"1997","DOI":"10.1002\/cbic.201900787","article-title":"Functional Characterisation of ClpP\nMutations Conferring Resistance to Acyldepsipeptide Antibiotics in\nFirmicutes","volume":"21","author":"Malik","year":"2020","journal-title":"Chembiochem"},{"issue":"3","key":"2026111011123578400_cit31","doi-asserted-by":"publisher","first-page":"515","DOI":"10.18433\/J3XC7S","article-title":"Quantitative\nstructure\u2013pharmacokinetics\nrelationships analysis of basic drugs: volume of distribution","volume":"18","author":"Zhivkova","year":"2015","journal-title":"J. Pharm. Pharm. Sci."},{"issue":"1","key":"2026111011123578400_cit32","doi-asserted-by":"publisher","first-page":"42717","DOI":"10.1038\/srep42717","article-title":"SwissADME: a free web tool to evaluate\npharmacokinetics, drug-likeness and medicinal chemistry friendliness\nof small molecules","volume":"7","author":"Daina","year":"2017","journal-title":"Sci. Rep."},{"key":"2026111011123578400_cit33","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1007\/s11030-011-9317-2","article-title":"An integrated drug-likeness study for bicyclic privileged structures:\nfrom physicochemical properties to in vitro ADME properties","volume":"15","author":"Han","year":"2011","journal-title":"Mol. Diversity"},{"issue":"9","key":"2026111011123578400_cit34","doi-asserted-by":"publisher","first-page":"1397","DOI":"10.1089\/cmb.2019.0323","article-title":"Analysis of\nthe physicochemical properties of acaricides based on Lipinski\u2019s\nrule of five","volume":"27","author":"Chen","year":"2020","journal-title":"J. Comput. Biol."},{"issue":"1","key":"2026111011123578400_cit35","doi-asserted-by":"publisher","first-page":"22221","DOI":"10.1038\/s41598-022-26446-x","article-title":"In silico\nactivity and ADMET profiling of phytochemicals from Ethiopian indigenous\naloes using pharmacophore models","volume":"12","author":"Bultum","year":"2022","journal-title":"Sci. Rep"},{"issue":"5","key":"2026111011123578400_cit36","doi-asserted-by":"publisher","first-page":"1157","DOI":"10.1016\/j.drudis.2019.03.015","article-title":"ADMET modeling\napproaches in drug\ndiscovery","volume":"24","author":"Ferreira","year":"2019","journal-title":"Drug Discov Today"},{"issue":"7","key":"2026111011123578400_cit37","doi-asserted-by":"publisher","first-page":"503","DOI":"10.2174\/1389200222666210705122913","article-title":"ADMET Profiling in Drug Discovery and Development:\nPerspectives of In Silico, In Vitro and Integrated Approaches","volume":"22","author":"Daoud","year":"2021","journal-title":"Curr. Drug Metab"},{"issue":"Suppl 10","key":"2026111011123578400_cit38","doi-asserted-by":"publisher","first-page":"S10","DOI":"10.1002\/jcph.1284","article-title":"Developmental Changes in Pharmacokinetics and Pharmacodynamics","volume":"58","author":"van den Anker","year":"2018","journal-title":"J. Clin. Pharmacol."},{"issue":"1","key":"2026111011123578400_cit39","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.bcp.2013.09.007","article-title":"Pharmacokinetics","volume":"87","author":"Fan","year":"2014","journal-title":"Biochem. Pharmacol."},{"issue":"4","key":"2026111011123578400_cit40","doi-asserted-by":"publisher","first-page":"427","DOI":"10.3390\/antibiotics11040427","article-title":"Antimicrobial susceptibility testing: a comprehensive\nreview of currently used methods","volume":"11","author":"Gajic","year":"2022","journal-title":"Antibiotics"},{"issue":"7","key":"2026111011123578400_cit41","doi-asserted-by":"publisher","first-page":"924","DOI":"10.3390\/antibiotics11070924","article-title":"The Production of Antibiotics Must Be Reoriented: Repositioning\nOld Narrow-Spectrum Antibiotics, Developing New Microbiome-Sparing\nAntibiotics","volume":"11","author":"Diamantis","year":"2022","journal-title":"Antibiotics (Basel)"},{"issue":"1","key":"2026111011123578400_cit42","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1111\/nyas.14649","article-title":"The future\nof antibiotics begins with discovering new combinations","volume":"1496","author":"Zhu","year":"2021","journal-title":"Ann. N.Y. Acad. Sci."},{"issue":"8","key":"2026111011123578400_cit43","doi-asserted-by":"publisher","first-page":"4573","DOI":"10.1128\/AAC.02463-14","article-title":"Antagonism between bacteriostatic\nand bactericidal antibiotics is prevalent","volume":"58","author":"Ocampo","year":"2014","journal-title":"Antimicrob.\nAgents Chemother."},{"issue":"2","key":"2026111011123578400_cit44","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2115","article-title":"Quantification of Triphenyl-2H-tetrazoliumchloride\nReduction Activity in Bacterial Cells","volume":"7","author":"Defez","year":"2017","journal-title":"Bio Protoc"},{"issue":"3","key":"2026111011123578400_cit45","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1002\/fft2.44","article-title":"Guidelines\nfor cell viability assays","volume":"1","author":"Kamiloglu","year":"2020","journal-title":"Food Frontiers"},{"issue":"6132","key":"2026111011123578400_cit46","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1126\/science.1234769","article-title":"Targeted inhibition of mutant IDH2 in leukemia cells\ninduces cellular differentiation","volume":"340","author":"Wang","year":"2013","journal-title":"Science"},{"issue":"1","key":"2026111011123578400_cit47","doi-asserted-by":"publisher","first-page":"3445","DOI":"10.1038\/s41467-024-47536-6","article-title":"Wild-type IDH2 is a therapeutic target for triple-negative breast\ncancer","volume":"15","author":"Li","year":"2024","journal-title":"Nat. Commun."},{"issue":"8","key":"2026111011123578400_cit48","doi-asserted-by":"publisher","first-page":"2168","DOI":"10.3390\/en11082168","article-title":"Autoignition behavior of an ethanol-methylcellulose\ngel droplet in a hot environment","volume":"11","author":"Lee","year":"2018","journal-title":"Energies"},{"issue":"9","key":"2026111011123578400_cit49","doi-asserted-by":"publisher","first-page":"2162","DOI":"10.3390\/polym15092162","article-title":"Characterization of Agarose Gels in Solvent and Non-Solvent\nMedia","volume":"15","author":"Roux","year":"2023","journal-title":"Polymers"},{"issue":"1","key":"2026111011123578400_cit50","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2019.188319","article-title":"Harnessing the\ntherapeutic potential of anticancer drugs through amorphous solid\ndispersions","volume":"1873","author":"Gala","year":"2020","journal-title":"Biochim Biophys Acta Rev. Cancer"},{"key":"2026111011123578400_cit51","doi-asserted-by":"publisher","first-page":"1121","DOI":"10.1016\/B978-1-4160-3291-5.50085-8","article-title":"Therapeutics","author":"Winston","year":"2009","journal-title":"ENDOCARDITIS."},{"issue":"6","key":"2026111011123578400_cit52","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1093\/jac\/dkl393","article-title":"Bactericidal\nagents in the treatment of MRSA infections\u2013the potential role\nof daptomycin","volume":"58","author":"French","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"issue":"8","key":"2026111011123578400_cit53","doi-asserted-by":"publisher","first-page":"ix","DOI":"10.1046\/j.1469-0691.2003.00790.x","article-title":"Determination of\nminimum inhibitory concentrations (MICs) of antibacterial agents by\nbroth dilution","volume":"9","author":"European Committee for Antimicrobial Susceptibility Testing (EUCAST) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID).","year":"2003","journal-title":"Clin. Microbiol. Infect."},{"key":"2026111011123578400_cit54","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1111\/j.1469-0691.1998.tb00061.x","article-title":"Methods\nfor the determination of susceptibility\nof bacteria to antimicrobial agents","volume":"4","author":"Guliy","year":"1998","journal-title":"Clin. Microbiol.\nInfect."},{"issue":"6","key":"2026111011123578400_cit55","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1016\/S0092-8674(01)00286-0","article-title":"Structural\nmechanism for rifampicin\ninhibition of bacterial RNA polymerase","volume":"104","author":"Campbell","year":"2001","journal-title":"Cell"},{"issue":"2","key":"2026111011123578400_cit56","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1093\/cid\/ciy645","article-title":"Static\nvs Cidal Antibiotics","volume":"68","author":"Steigbigel","year":"2019","journal-title":"Clin Infect Dis"},{"key":"2026111011123578400_cit57","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2021.104840","article-title":"The effect of EDTA\nin combination with some antibiotics against clinical isolates of\ngram negative bacteria in Mansoura","volume":"154","author":"Aboelenin","year":"2021","journal-title":"Egypt."},{"key":"2026111011123578400_cit58","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1014169","article-title":"Antibacterial and antibiofilm effects\nof essential oil components, EDTA and HLE disinfectant solution on\nEnterococcus, Pseudomonas and Staphylococcus sp. multiresistant strains\nisolated along the meat production chain","volume":"13","author":"Caballero Go\u0301mez","year":"2022","journal-title":"Front.\nMicrobiol."},{"issue":"10","key":"2026111011123578400_cit59","doi-asserted-by":"publisher","first-page":"688","DOI":"10.3390\/antibiotics9100688","article-title":"Antibiotics in food chain: The consequences\nfor antibiotic resistance","volume":"9","author":"Kumar","year":"2020","journal-title":"Antibiotics"},{"issue":"36","key":"2026111011123578400_cit60","doi-asserted-by":"publisher","first-page":"40724","DOI":"10.1021\/acsami.2c12943","article-title":"Hetero-multivalent\ntargeted liposomal drug delivery to treat Pseudomonas aeruginosa infections","volume":"14","author":"Singla","year":"2022","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"1","key":"2026111011123578400_cit61","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1038\/s41596-022-00755-x","article-title":"Preparation of selective organ-targeting\n(SORT) lipid nanoparticles (LNPs) using multiple technical methods\nfor tissue-specific mRNA delivery","volume":"18","author":"Wang","year":"2023","journal-title":"Nat. Protocols"},{"issue":"D1","key":"2026111011123578400_cit62","doi-asserted-by":"publisher","first-page":"D1373","DOI":"10.1093\/nar\/gkac956","article-title":"PubChem 2023 update","volume":"51","author":"Kim","year":"2023","journal-title":"Nucleic acids Res."},{"key":"2026111011123578400_cit63","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00894-018-3677-9","article-title":"Construction of a molecular structure model of mild-oxidized Chinese\nlignite using Gaussian09 based on data from FTIR, solid state 13 C-NMR","volume":"24","author":"Lu","year":"2018","journal-title":"J. Mol. Model."},{"issue":"suppl_1","key":"2026111011123578400_cit64","doi-asserted-by":"publisher","first-page":"D302","DOI":"10.1093\/nar\/gkj120","article-title":"The RCSB PDB\ninformation portal for structural genomics","volume":"34","author":"Kouranov","year":"2006","journal-title":"Nucleic Acids Res."},{"issue":"2","key":"2026111011123578400_cit65","doi-asserted-by":"publisher","DOI":"10.1002\/wcms.1298","article-title":"Using PyMOL as a platform for computational\ndrug design","volume":"7","author":"Yuan","year":"2017","journal-title":"Wiley Interdiscip. Rev.:Comput.\nMol. Sci."},{"issue":"15","key":"2026111011123578400_cit66","doi-asserted-by":"publisher","first-page":"2714","DOI":"10.1002\/elps.1150181505","article-title":"SWISS-MODEL and\nthe Swiss-Pdb Viewer: an environment\nfor comparative protein modeling","volume":"18","author":"Guex","year":"1997","journal-title":"Electrophoresis"},{"issue":"W1","key":"2026111011123578400_cit67","doi-asserted-by":"publisher","first-page":"W363","DOI":"10.1093\/nar\/gky473","article-title":"CASTp 3.0:\ncomputed atlas of surface topography of proteins","volume":"46","author":"Tian","year":"2018","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"2026111011123578400_cit68","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1021\/acssynbio.7b00122","article-title":"Crosstalk between Diverse Synthetic Protein Degradation\nTags in Escherichia coli","volume":"7","author":"Butzin","year":"2018","journal-title":"ACS Synth. Biol."},{"key":"2026111011123578400_cit69","doi-asserted-by":"publisher","first-page":"1062","DOI":"10.1021\/acssynbio.4c00612","article-title":"Engineering a New SsrA-Based Degradation\nTag (LAA-LAA) and a Bacterial Synthetic Oscillator","volume":"14","author":"Jadhav","year":"2025","journal-title":"ACS Synth. Biol."},{"issue":"1","key":"2026111011123578400_cit70","doi-asserted-by":"publisher","first-page":"782","DOI":"10.1038\/s41598-023-51014-2","article-title":"Counter-on-chip for bacterial cell quantification,\ngrowth, and live-dead estimations","volume":"14","author":"Rahman","year":"2024","journal-title":"Sci. Rep"},{"issue":"1","key":"2026111011123578400_cit71","doi-asserted-by":"publisher","first-page":"16071","DOI":"10.1038\/s41598-017-16176-w","article-title":"The spatiotemporal\nsystem dynamics\nof acquired resistance in an engineered microecology","volume":"7","author":"Datla","year":"2017","journal-title":"Sci. Rep."},{"issue":"1","key":"2026111011123578400_cit72","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1021\/acssynbio.9b00358","article-title":"Proteolytic\nqueues at ClpXP increase antibiotic tolerance","volume":"9","author":"Deter","year":"2019","journal-title":"ACS Synth. Biol."},{"key":"2026111011123578400_cit73","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102391","article-title":"Antibiotic\ntolerance,\npersistence, and resistance of the evolved minimal cell, Mycoplasma\nmycoides JCVI-Syn3B","volume":"24","author":"Hossain","year":"2021","journal-title":"Iscience"},{"issue":"5","key":"2026111011123578400_cit74","doi-asserted-by":"publisher","first-page":"e01219","DOI":"10.1128\/spectrum.01219-23","article-title":"Escherichia\ncoli cells\nare primed for survival before lethal antibiotic stress","volume":"11","author":"Hossain","year":"2023","journal-title":"Microbiol. Spectr."},{"issue":"1","key":"2026111011123578400_cit75","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2024.107386","article-title":"Rethinking dormancy: Antibiotic persisters\nare metabolically\nactive, non-growing cells","volume":"65","author":"Rahman","year":"2025","journal-title":"Int. J. Antimicrob.\nAgents"},{"issue":"1","key":"2026111011123578400_cit76","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1038\/s42003-025-07639-2","article-title":"Priestia\nmegaterium cells are primed\nfor surviving lethal doses of antibiotics and chemical stress","volume":"8","author":"Guha","year":"2025","journal-title":"Commun. Biol."},{"issue":"1","key":"2026111011123578400_cit77","first-page":"1","article-title":"Kirby-Bauer\ndisk diffusion susceptibility test protocol","volume":"15","author":"Hudzicki","year":"2009","journal-title":"Am. Soc. Microbiol."}],"container-title":["ACS Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsinfecdis.5c00666","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/11\/12\/3520\/69146268\/acsinfecdis.5c00666.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/11\/12\/3520\/69146268\/acsinfecdis.5c00666.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,11,10]],"date-time":"2026-11-10T16:22:26Z","timestamp":1794327746000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article\/11\/12\/3520\/3663140\/Repurposing-AGI-6780-to-Activate-ClpP-and-Target"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,10]]},"references-count":77,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2025,11,10]]},"published-print":{"date-parts":[[2025,12,12]]}},"URL":"https:\/\/doi.org\/10.1021\/acsinfecdis.5c00666","relation":{"has-part":[{"id-type":"doi","id":"https:\/\/doi.org\/10.1021\/acsinfecdis.5c00666.s001","asserted-by":"subject"}]},"ISSN":["2373-8227"],"issn-type":[{"value":"2373-8227","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,10]]}}}