{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T04:01:11Z","timestamp":1793937671599,"version":"4.8.0"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"5","license":[{"start":{"date-parts":[[2017,3,23]],"date-time":"2017-03-23T00:00:00Z","timestamp":1490227200000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100007212","name":"V\u00e4stra G\u00f6talandsregionen","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100007212","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007464","name":"Torsten Soderbergs Stiftelse","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100007464","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["634890"],"award-info":[{"award-number":["634890"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003743","name":"Adlerbertska Stiftelserna","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100003743","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2014-4305"],"award-info":[{"award-number":["2014-4305"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005754","name":"Sahlgrenska Universitetssjukhuset","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100005754","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"EuroNanoMed2","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,5,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Resistance to life-saving antibiotics increases rapidly worldwide, and multiresistant bacteria have become a global threat to human health. Presently, the most serious threat is the increasing spread of Enterobacteriaceae carrying genes coding for extended spectrum \u03b2-lactamases (ESBL) and carbapenemases on highly mobile plasmids. We here demonstrate how optical DNA maps of single plasmids can be used as fingerprints to trace plasmids, for example, during resistance outbreaks. We use the assay to demonstrate a potential transmission route of an ESBL-carrying plasmid between bacterial strains\/species and between patients, during a polyclonal outbreak at a neonatal ward at Sahlgrenska University Hospital (Gothenburg, Sweden). Our results demonstrate that optical DNA mapping is an easy and rapid method for detecting the spread of plasmids mediating resistance. With the increasing prevalence of multiresistant bacteria, diagnostic tools that can aid in solving ongoing routes of transmission, in particular in hospital settings, will be of paramount importance.<\/jats:p>","DOI":"10.1021\/acsinfecdis.6b00017","type":"journal-article","created":{"date-parts":[[2016,3,23]],"date-time":"2016-03-23T20:29:53Z","timestamp":1458764993000},"page":"322-328","source":"Crossref","is-referenced-by-count":36,"title":["Rapid Tracing of Resistance Plasmids in a Nosocomial Outbreak Using\nOptical DNA Mapping"],"prefix":"10.1021","volume":"2","author":[{"given":"Vilhelm","family":"Mu\u0308ller","sequence":"first","affiliation":[{"name":"Chalmers University of Technology , , Kemiva\u0308gen 10 , ,","place":["Gothenburg, Sweden, 41296"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahid","family":"Karami","sequence":"additional","affiliation":[{"name":"University of Gothenburg , , Guldhedsgatan 10 , ,","place":["Gothenburg, Sweden, 41346"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena K.","family":"Nyberg","sequence":"additional","affiliation":[{"name":"Chalmers University of Technology , , Kemiva\u0308gen 10 , ,","place":["Gothenburg, Sweden, 41296"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoffer","family":"Pichler","sequence":"additional","affiliation":[{"name":"Lund University , , So\u0308lvegatan 14A , ,","place":["Lund, Sweden, 22362"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola C.","family":"Torche Pedreschi","sequence":"additional","affiliation":[{"name":"Lund University , , So\u0308lvegatan 14A , ,","place":["Lund, Sweden, 22362"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saair","family":"Quaderi","sequence":"additional","affiliation":[{"name":"Chalmers University of Technology , , Kemiva\u0308gen 10 , ,","place":["Gothenburg, Sweden, 41296"]},{"name":"Lund University , , So\u0308lvegatan 14A , ,","place":["Lund, Sweden, 22362"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Fritzsche","sequence":"additional","affiliation":[{"name":"Chalmers University of Technology , , Kemiva\u0308gen 9 , ,","place":["Gothenburg, Sweden, 41296"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Ambjo\u0308rnsson","sequence":"additional","affiliation":[{"name":"Lund University , , So\u0308lvegatan 14A , ,","place":["Lund, Sweden, 22362"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"\u00c5hre\u0301n","sequence":"additional","affiliation":[{"name":"University of Gothenburg , , Guldhedsgatan 10 , ,","place":["Gothenburg, Sweden, 41346"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredrik","family":"Westerlund","sequence":"additional","affiliation":[{"name":"Chalmers University of Technology , , Kemiva\u0308gen 10 , ,","place":["Gothenburg, Sweden, 41296"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2016,3,23]]},"reference":[{"key":"2026090303542156800_cit1","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1016\/S1473-3099(13)70318-9","article-title":"Antibiotic resistance \u2013 the need for global solutions","volume":"13","author":"Laxminarayan","year":"2013","journal-title":"Lancet Infect. Dis."},{"key":"2026090303542156800_cit2","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1186\/1471-2148-11-130","article-title":"The evolution of plasmid-carried\nantibiotic resistance","volume":"11","author":"Svara","year":"2011","journal-title":"BMC Evol. Biol."},{"key":"2026090303542156800_cit3","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1016\/j.ijmm.2013.02.001","article-title":"Plasmids and the spread of resistance","volume":"303","author":"Carattoli","year":"2013","journal-title":"Int. J. Med. Microbiol."},{"key":"2026090303542156800_cit4","unstructured":"World Health Organization.\n          \n          2015)  Worldwide\nCountry Situation Analysis: Response to Antimicrobial Resistance."},{"key":"2026090303542156800_cit5","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1128\/CMR.00116-14","article-title":"The role\nof epidemic resistance plasmids and international high-risk clones\nin the spread of multidrug-resistant Enterobacteriaceae","volume":"28","author":"Mathers","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"2026090303542156800_cit6","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1006\/abio.1995.1220","article-title":"A general method for detecting and\nsizing large plasmids","volume":"226","author":"Barton","year":"1995","journal-title":"Anal. Biochem."},{"key":"2026090303542156800_cit7","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1016\/j.ijmm.2011.09.003","article-title":"Plasmids in Gram negatives: molecular\ntyping of resistance plasmids","volume":"301","author":"Carattoli","year":"2011","journal-title":"Int. J. Med.\nMicrobiol."},{"key":"2026090303542156800_cit8","doi-asserted-by":"publisher","first-page":"18","DOI":"10.3109\/23744235.2015.1062536","article-title":"Characterization of ESBL disseminating plasmids","volume":"48","author":"Brolund","year":"2016","journal-title":"Infect. Dis. (London, England)"},{"key":"2026090303542156800_cit9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ygeno.2015.11.003","article-title":"The sequence of sequencers: the history\nof sequencing DNA","volume":"107","author":"Heather","year":"2016","journal-title":"Genomics"},{"key":"2026090303542156800_cit10","doi-asserted-by":"publisher","first-page":"e118","DOI":"10.1093\/nar\/gku556","article-title":"Competitive binding-based optical DNA mapping for fast\nidentification of bacteria \u2013 multi-ligand transfer matrix theory\nand experimental applications on Escherichia coli","volume":"42","author":"Nilsson","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"2026090303542156800_cit11","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1016\/j.bbrc.2011.11.128","article-title":"A single-step\ncompetitive binding assay for mapping of single DNA molecules","volume":"417","author":"Nyberg","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026090303542156800_cit12","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1002\/bip.21579","article-title":"Optical mapping\nof DNA: single-molecule-based methods for mapping genomes","volume":"95","author":"Neely","year":"2011","journal-title":"Biopolymers"},{"key":"2026090303542156800_cit13","doi-asserted-by":"publisher","first-page":"1133","DOI":"10.1039\/b820266b","article-title":"DNA manipulation, sorting, and mapping in nanofluidic\nsystems","volume":"39","author":"Levy","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"2026090303542156800_cit14","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1039\/b912918a","article-title":"DNA in\nnanochannels \u2013 directly visualizing genomic information","volume":"39","author":"Persson","year":"2010","journal-title":"Chem. Soc. Rev."},{"key":"2026090303542156800_cit15","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1021\/ma5022067","article-title":"Nanoconfined\ncircular and linear DNA: equilibrium conformations and unfolding kinetics","volume":"48","author":"Alizadehheidari","year":"2015","journal-title":"Macromolecules"},{"key":"2026090303542156800_cit16","doi-asserted-by":"publisher","first-page":"2739","DOI":"10.1039\/C5LC00378D","article-title":"Fast size-determination of intact bacterial plasmids\nusing nanofluidic channels","volume":"15","author":"Frykholm","year":"2015","journal-title":"Lab Chip"},{"key":"2026090303542156800_cit17","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1128\/MMBR.00020-10","article-title":"Mobility of\nplasmids","volume":"74","author":"Smillie","year":"2010","journal-title":"Microbiol. Mol. Biol. Rev.: MMBR"},{"key":"2026090303542156800_cit18","doi-asserted-by":"publisher","first-page":"e83030","DOI":"10.1371\/journal.pone.0083030","article-title":"Sub-typing of extended-spectrum-\u03b2-lactamase-producing\nisolates from a nosocomial outbreak: application of a 10-loci generic Escherichia coli multi-locus variable number tandem repeat\nanalysis","volume":"8","author":"Karami","year":"2013","journal-title":"PLoS One"},{"key":"2026090303542156800_cit19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/jac\/dkq415","article-title":"Escherichia coli O25b-ST131: a pandemic, multiresistant, community-associated\nstrain","volume":"66","author":"Rogers","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"2026090303542156800_cit33","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1128\/jb.131.3.929-942.1977","article-title":"Origin and direction of replication of the drug resistance plasmid\nR100.1 and of a resistance transfer factor derivative in synchronized\ncultures","volume":"131","author":"Silver","year":"1977","journal-title":"J. Bacteriol."},{"key":"2026090303542156800_cit20","doi-asserted-by":"publisher","first-page":"E87","DOI":"10.1111\/1469-0691.12086","article-title":"Shift of CTX-M genotypes has determined\nthe increased prevalence of extended-spectrum beta-lactamase-producing Escherichia coli in south-western Sweden","volume":"19","author":"Helldal","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"2026090303542156800_cit21","doi-asserted-by":"publisher","first-page":"18","DOI":"10.3109\/23744235.2015.1062536","article-title":"Characterization\nof ESBL disseminating plasmids","volume":"48","author":"Brolund","year":"2016","journal-title":"Infect. Dis.\n(London, England)"},{"key":"2026090303542156800_cit22","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1039\/C0LC00260G","article-title":"Injection molded nanofluidic chips: fabrication method\nand functional tests using single-molecule DNA experiments","volume":"11","author":"Utko","year":"2011","journal-title":"Lab Chip"},{"key":"2026090303542156800_cit23","doi-asserted-by":"publisher","first-page":"105002","DOI":"10.1088\/0960-1317\/25\/10\/105002","article-title":"Optical mapping of single-molecule\nhuman DNA in disposable, mass-produced all-polymer devices","volume":"25","author":"Peter Friis","year":"2015","journal-title":"J. Micromech. Microeng."},{"key":"2026090303542156800_cit24","doi-asserted-by":"publisher","first-page":"12725","DOI":"10.1021\/nn505821y","article-title":"Imaging and sizing\nof single DNA molecules on a mobile phone","volume":"8","author":"Wei","year":"2014","journal-title":"ACS\nNano"},{"key":"2026090303542156800_cit25","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1007\/BF01967473","article-title":"Detection of extended broad-spectrum beta-lactamases\nin Enterobacteriaceae in four French hospitals","volume":"8","author":"Legrand","year":"1989","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"2026090303542156800_cit26","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1099\/jmm.0.46909-0","article-title":"Real-time TaqMan PCR for rapid detection and typing\nof genes encoding CTX-M extended-spectrum beta-lactamases","volume":"56","author":"Birkett","year":"2007","journal-title":"J. Med. Microbiol."},{"key":"2026090303542156800_cit27","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1111\/j.1348-0421.2009.00168.x","article-title":"Characterization\nof integrons and antimicrobial resistance genes in clinical isolates\nof Gram-negative bacteria from Palestinian hospitals","volume":"53","author":"Hussein","year":"2009","journal-title":"Microbiol. Immunol."},{"key":"2026090303542156800_cit28","doi-asserted-by":"crossref","first-page":"2977","DOI":"10.1128\/jcm.35.11.2977-2980.1997","article-title":"Rapid pulsed-field gel electrophoresis\nprotocol for typing of Escherichia coli O157:H7 and\nother Gram-negative organisms in 1 day","volume":"35","author":"Gautom","year":"1997","journal-title":"J. Clin.\nMicrobiol."},{"key":"2026090303542156800_cit29","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1007\/s100960000380","article-title":"Improved microbiological techniques\nusing the polymerase chain reaction and pulsed-field gel electrophoresis\nfor diagnosis and follow-up of enterohaemorrhagic Escherichia coli infection","volume":"19","author":"Welinder-Olsson","year":"2000","journal-title":"Eur. J. Clin. Microbiol.\nInfect. Dis."},{"issue":"Suppl. 3","key":"2026090303542156800_cit30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1469-0691.2007.01786.x","article-title":"Guidelines\nfor the validation and application of typing methods for use in bacterial\nepidemiology","volume":"13","author":"van Belkum","year":"2007","journal-title":"Clin. Microbiol. Infect."},{"key":"2026090303542156800_cit31","doi-asserted-by":"publisher","first-page":"1136","DOI":"10.1111\/j.1365-2958.2006.05172.x","article-title":"Sex and virulence\nin Escherichia coli: an evolutionary perspective","volume":"60","author":"Wirth","year":"2006","journal-title":"Mol. Microbiol."},{"key":"2026090303542156800_cit32","doi-asserted-by":"publisher","first-page":"4178","DOI":"10.1128\/JCM.43.8.4178-4182.2005","article-title":"Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates","volume":"43","author":"Diancourt","year":"2005","journal-title":"J. Clin. Microbiol."}],"container-title":["ACS Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsinfecdis.6b00017","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/2\/5\/322\/67182785\/acsinfecdis.6b00017.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/2\/5\/322\/67182785\/acsinfecdis.6b00017.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:54:27Z","timestamp":1788422067000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article\/2\/5\/322\/788281\/Rapid-Tracing-of-Resistance-Plasmids-in-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,23]]},"references-count":33,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2016,3,23]]},"published-print":{"date-parts":[[2016,5,13]]}},"URL":"https:\/\/doi.org\/10.1021\/acsinfecdis.6b00017","relation":{},"ISSN":["2373-8227"],"issn-type":[{"value":"2373-8227","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,23]]}}}