{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T15:28:57Z","timestamp":1786721337830,"version":"build-2736575974"},"reference-count":49,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:00:00Z","timestamp":1784332800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:00:00Z","timestamp":1784332800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:00:00Z","timestamp":1784332800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation (NSF)","doi-asserted-by":"crossref","award":["CHE-2316599"],"award-info":[{"award-number":["CHE-2316599"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Antibiotic-resistant bacteria are one of the greatest challenges in modern medicine, as multidrug-resistant strains continue to outpace traditional antibiotic development. Unlike conventional antibiotics that promote resistance through selective pressure, quorum sensing-targeted therapies disrupt bacterial communication systems that regulate, among other traits, virulence, biofilm formation, and resistance acquirement through competence without directly killing the bacteria. In this study we examined quorum sensing modulation in Streptococcus mitis, a commensal oral bacterium that has recently been identified as an opportunistic pathogen capable of causing serious infections in immunocompromised individuals. The competence regulon quorum sensing system in S. mitis is controlled by the competence-stimulating peptide (CSP), which activates the histidine kinase receptor ComD to drive quorum sensing-regulated processes. S. mitis strains can be divided into different specificity groups, or pherotypes, based on the CSP they produce and cognate ComD receptor. In this work we set out to define the molecular interactions that drive CSP1\/ComD1 binding and lead to ComD1 activation by conducting a systematic structure\u2013activity analysis of the S. mitis-CSP1 sequence. To this end, we synthesized and screened two S. mitis-CSP1 analog libraries: the first, an alanine scan to identify key side-chain residues responsible for activity, and the second, a d-amino acid scan to evaluate the effect of side chain spatial orientation. Then, following the construction of a luminescence-based S. mitis NCTC 8033 quorum sensing reporter strain, we conducted reporter gene bioassays to gain insights into the structure\u2013activity relationship between S. mitis-CSP1 and its cognate ComD1 receptor. Interestingly, our results revealed that the glutamic acid-1-to-alanine analog (S. mitis-CSP1-E1A) had increased potency but lower efficacy, indicating partial agonist effects in S. mitis NCTC 8033, whereas the same glutamic acid-1-to-alanine substitution in CSPs from other Streptococcus species, as well as in a different S. mitis pherotype producing and responding to S. mitis-CSP2, has been reported to produce inhibitory analogs. These results suggest that the ComD1 receptor in S. mitis NCTC 8033 interacts with S. mitis-CSP1 differently than its homologues in other Streptococcus species, highlight the importance of species-specific studies, and caution against overgeneralizing bacterial behavior within a given genus. Overall, our work offers mechanistic insight into S. mitis quorum sensing signaling and lays a foundation for the rational development of CSP-based tools to study and potentially control quorum sensing-regulated processes in streptococci.<\/jats:p>","DOI":"10.1021\/acsinfecdis.6c00548","type":"journal-article","created":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T13:24:12Z","timestamp":1784381052000},"page":"2863-2873","source":"Crossref","is-referenced-by-count":0,"title":["Structure\u2013Activity\nAnalysis of the Competence\nStimulating Peptide-1 in\n                    <i>Streptococcus mitis<\/i>"],"prefix":"10.1021","volume":"12","author":[{"given":"Morgan M.","family":"Yeager","sequence":"first","affiliation":[{"name":"University of Nevada , , Reno, 1664 North Virginia Street , , ,","place":["Reno, Nevada, United States, 89557"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April K.","family":"Murray","sequence":"additional","affiliation":[{"name":"University of Nevada , , Reno, 1664 North Virginia Street , , ,","place":["Reno, Nevada, United States, 89557"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ripon C.","family":"Bhowmic","sequence":"additional","affiliation":[{"name":"University of Nevada , , Reno, 1664 North Virginia Street , , ,","place":["Reno, Nevada, United States, 89557"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9917-274X","authenticated-orcid":true,"given":"Clay P.","family":"Renshaw","sequence":"additional","affiliation":[{"name":"University of Nevada , , Reno, 1664 North Virginia Street , , ,","place":["Reno, Nevada, United States, 89557"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahmina A.","family":"Milly","sequence":"additional","affiliation":[{"name":"University of Nevada , , Reno, 1664 North Virginia Street , , ,","place":["Reno, Nevada, United States, 89557"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2052-6782","authenticated-orcid":true,"given":"Yftah","family":"Tal-Gan","sequence":"additional","affiliation":[{"name":"University of Nevada , , Reno, 1664 North Virginia Street , , ,","place":["Reno, Nevada, United States, 89557"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,18]]},"reference":[{"issue":"1","key":"2026081410080497000_cit1","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1038\/s41392-022-00974-4","article-title":"Microbiota in health and diseases","volume":"7","author":"Hou","year":"2022","journal-title":"Signal Transduction Targeted Ther."},{"issue":"1","key":"2026081410080497000_cit2","doi-asserted-by":"publisher","first-page":"122","DOI":"10.4103\/jomfp.JOMFP_304_18","article-title":"Oral microbiome: Unveiling the fundamentals","volume":"23","author":"Deo","year":"2019","journal-title":"Int. J. Oral Maxillofac. Pathol."},{"issue":"1","key":"2026081410080497000_cit3","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1111\/prd.12393","article-title":"The oral\nmicrobiome: Role of key organisms and complex networks in oral health\nand disease","volume":"87","author":"Sedghi","year":"2021","journal-title":"Periodontol. 2000"},{"issue":"11","key":"2026081410080497000_cit4","doi-asserted-by":"publisher","first-page":"1626","DOI":"10.1172\/JCI20430","article-title":"Quorum\nsensing and biofilm formation\nin Streptococcal infections","volume":"112","author":"Cvitkovitch","year":"2003","journal-title":"J. Clin. Invest."},{"issue":"6","key":"2026081410080497000_cit5","doi-asserted-by":"publisher","first-page":"1544","DOI":"10.1093\/cid\/ciae168","article-title":"Predictors\nof Mortality of Streptococcal\nBacteremia and the Role of Infectious Diseases Consultation: A Retrospective\nCohort Study","volume":"78","author":"Fourre","year":"2024","journal-title":"Clin. Infect. Dis."},{"issue":"4","key":"2026081410080497000_cit6","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1128\/CMR.15.4.613-630.2002","article-title":"What happened\nto the streptococci: overview of taxonomic and nomenclature changes","volume":"15","author":"Facklam","year":"2002","journal-title":"Clin. Microbiol. Rev."},{"issue":"3","key":"2026081410080497000_cit7","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1080\/21505594.2016.1159366","article-title":"Epidemiology\nof antimicrobial resistance in bloodstream infections","volume":"7","author":"Akova","year":"2016","journal-title":"Virulence"},{"issue":"7","key":"2026081410080497000_cit8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002683","article-title":"Evolution\nof Streptococcus pneumoniae and its close commensal relatives","volume":"3","author":"Kilian","year":"2008","journal-title":"PLoS One"},{"issue":"4","key":"2026081410080497000_cit9","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01490-14","article-title":"Parallel evolution of Streptococcus pneumoniae and\nStreptococcus mitis to pathogenic and mutualistic lifestyles","volume":"5","author":"Kilian","year":"2014","journal-title":"mBio"},{"key":"2026081410080497000_cit10","doi-asserted-by":"publisher","first-page":"747","DOI":"10.3389\/fimmu.2018.00747","article-title":"Antibodies Reactive to Commensal Streptococcus mitis\nShow Cross-Reactivity With Virulent Streptococcus pneumoniae Serotypes","volume":"9","author":"Shekhar","year":"2018","journal-title":"Front. Immunol."},{"issue":"2","key":"2026081410080497000_cit11","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1039\/D0CB00012D","article-title":"Biological Evaluation\nof Native Streptococcal Competence\nStimulating Peptides Reveal Potential Crosstalk Between Streptococcus mitis and Streptococcus pneumoniae and a\nNew Scaffold for the Development of S. pneumoniae Quorum Sensing Modulators","volume":"1","author":"Milly","year":"2020","journal-title":"RSC Chem. Biol."},{"issue":"7","key":"2026081410080497000_cit12","doi-asserted-by":"publisher","first-page":"1222","DOI":"10.3390\/antibiotics12071222","article-title":"Streptococcus mitis\nas a New Emerging Pathogen in Pediatric Age: Case Report and Systematic\nReview","volume":"12","author":"Colomba","year":"2023","journal-title":"Antibiotics"},{"issue":"2","key":"2026081410080497000_cit13","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1111\/j.2041-1014.2010.00601.x","article-title":"Streptococcus\nmitis: walking the line between commensalism and pathogenesis","volume":"26","author":"Mitchell","year":"2011","journal-title":"Mol. Oral Microbiol."},{"key":"2026081410080497000_cit14","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1097\/00001432-199712000-00002","article-title":"Virulent infections\ncaused by alpha-haemolytic\nstreptococci in cancer patients and their management","volume":"10","author":"Bochud","year":"1997","journal-title":"Curr. Opin. Infect. Dis."},{"issue":"1","key":"2026081410080497000_cit15","doi-asserted-by":"publisher","first-page":"7812","DOI":"10.1038\/s41467-024-52120-z","article-title":"Population\ngenomics of Streptococcus mitis in UK and Ireland bloodstream infection\nand infective endocarditis cases","volume":"15","author":"Kalizang\u2019oma","year":"2024","journal-title":"Nat. Commun."},{"issue":"4","key":"2026081410080497000_cit16","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1128\/AAC.40.4.891","article-title":"Emergence of high\nrates of antimicrobial resistance among viridans group streptococci\nin the United States","volume":"40","author":"Doern","year":"1996","journal-title":"Antimicrob. Agents Chemother."},{"issue":"12","key":"2026081410080497000_cit17","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1111\/1348-0421.13028","article-title":"Oral mitis group streptococci:\nA silent majority in our oral cavity","volume":"66","author":"Okahashi","year":"2022","journal-title":"Microbiol.\nImmunol."},{"issue":"2","key":"2026081410080497000_cit18","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1111\/j.2041-1014.2011.00635.x","article-title":"Identification\nof a functional capsule\nlocus in Streptococcus mitis","volume":"27","author":"Rukke","year":"2012","journal-title":"Mol. Oral Microbiol."},{"issue":"20","key":"2026081410080497000_cit19","doi-asserted-by":"publisher","first-page":"6087","DOI":"10.1128\/jb.178.20.6087-6090.1996","article-title":"Competence\nfor genetic transformation in encapsulated strains of Streptococcus\npneumoniae: two allelic variants of the peptide pheromone","volume":"178","author":"Pozzi","year":"1996","journal-title":"J. Bacteriol."},{"issue":"1","key":"2026081410080497000_cit20","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1007\/s00294-015-0520-z","article-title":"Disentangling\ncompetence for genetic transformation\nand virulence in Streptococcus pneumoniae","volume":"62","author":"Lin","year":"2016","journal-title":"Curr.\nGenet."},{"issue":"10","key":"2026081410080497000_cit21","doi-asserted-by":"publisher","first-page":"R107","DOI":"10.1186\/gb-2010-11-10-r107","article-title":"Structure\nand dynamics of the pan-genome\nof Streptococcus pneumoniae and closely related species","volume":"11","author":"Donati","year":"2010","journal-title":"Genome Biol."},{"key":"2026081410080497000_cit22","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1146\/annurev.micro.55.1.165","article-title":"Quorum sensing in\nbacteria","volume":"55","author":"Miller","year":"2001","journal-title":"Annu.\nRev. Microbiol."},{"key":"2026081410080497000_cit23","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1146\/annurev.cellbio.21.012704.131001","article-title":"Quorum sensing:\ncell-to-cell communication in bacteria","volume":"21","author":"Waters","year":"2005","journal-title":"Annu.\nRev. Cell Dev. Biol."},{"key":"2026081410080497000_cit24","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1016\/s0065-2911(01)45005-3","article-title":"Quorum sensing as a\npopulation-density-dependent determinant\nof bacterial physiology","volume":"45","author":"Swift","year":"2001","journal-title":"Adv. Microb. Physiol."},{"issue":"3","key":"2026081410080497000_cit25","doi-asserted-by":"publisher","first-page":"425","DOI":"10.3390\/microorganisms8030425","article-title":"Quorum-Sensing\nRegulation of Antimicrobial Resistance\nin Bacteria","volume":"8","author":"Zhao","year":"2020","journal-title":"Microorganisms"},{"issue":"16","key":"2026081410080497000_cit26","doi-asserted-by":"publisher","first-page":"5004","DOI":"10.1128\/JB.181.16.5004-5016.1999","article-title":"Identification of a new regulator\nin Streptococcus\npneumoniae linking quorum sensing to competence for genetic transformation","volume":"181","author":"Lee","year":"1999","journal-title":"J. Bacteriol."},{"issue":"1","key":"2026081410080497000_cit27","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1186\/s12864-018-4802-y","article-title":"High-resolution\nprofiles of the Streptococcus mitis\nCSP signaling pathway reveal core and strain-specific regulated genes","volume":"19","author":"Salvadori","year":"2018","journal-title":"BMC Genomics"},{"issue":"4","key":"2026081410080497000_cit28","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1128\/MMBR.69.4.527-543.2005","article-title":"Alternative\nsigma factors and their\nroles in bacterial virulence","volume":"69","author":"Kazmierczak","year":"2005","journal-title":"Microbiol. Mol.\nBiol. Rev."},{"issue":"6","key":"2026081410080497000_cit29","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1016\/j.mib.2008.09.020","article-title":"Stochastic switching\nto competence","volume":"11","author":"Leisner","year":"2008","journal-title":"Curr. Opin. Microbiol."},{"issue":"1","key":"2026081410080497000_cit30","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1111\/mmi.13016","article-title":"Deletion analysis\nof Streptococcus pneumoniae late competence genes distinguishes virulence\ndeterminants that are dependent or independent of competence induction","volume":"97","author":"Zhu","year":"2015","journal-title":"Mol. Microbiol."},{"key":"2026081410080497000_cit31","doi-asserted-by":"publisher","first-page":"94","DOI":"10.3389\/fcimb.2019.00094","article-title":"Competence in Streptococcus\npneumoniae and Close Commensal Relatives: Mechanisms and Implications","volume":"9","author":"Salvadori","year":"2019","journal-title":"Front. Cell. Infect. Microbiol."},{"issue":"12","key":"2026081410080497000_cit32","doi-asserted-by":"publisher","first-page":"105448","DOI":"10.1016\/j.jbc.2023.105448","article-title":"Developing\nmultispecies quorum-sensing\nmodulators based on the Streptococcus mitis competence-stimulating\npeptide","volume":"299","author":"Milly","year":"2023","journal-title":"J. Biol. Chem."},{"issue":"4","key":"2026081410080497000_cit33","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/j.jiph.2016.08.007","article-title":"Antibiotic resistance","volume":"10","author":"Frieri","year":"2017","journal-title":"J. Infect. Public Health"},{"issue":"2","key":"2026081410080497000_cit34","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1046\/j.1365-2796.2002.01026.x","article-title":"Evolution and spread of antibiotic\nresistance","volume":"252","author":"Normark","year":"2002","journal-title":"J. Intern. Med."},{"key":"2026081410080497000_cit35","doi-asserted-by":"publisher","first-page":"3903","DOI":"10.2147\/IDR.S234610","article-title":"Antimicrobial\nResistance: Implications and Costs","volume":"12","author":"Dadgostar","year":"2019","journal-title":"Infect.\nDrug Resist."},{"key":"2026081410080497000_cit36","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1007\/82_2016_499","article-title":"History\nof Antibiotics Research","volume":"398","author":"Mohr","year":"2016","journal-title":"Curr. Top. Microbiol.\nImmunol."},{"key":"2026081410080497000_cit37","doi-asserted-by":"publisher","first-page":"102111","DOI":"10.1016\/j.bbrep.2025.102111","article-title":"Natural\nproduct-based inhibitors\nof quorum sensing: A novel approach to combat antibiotic resistance","volume":"43","author":"Alum","year":"2025","journal-title":"Biochem. Biophys. Rep."},{"key":"2026081410080497000_cit38","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.3389\/fmicb.2016.01009","article-title":"Overcoming the Barrier\nof Low Efficiency during Genetic Transformation of Streptococcus mitis","volume":"07","author":"Salvadori","year":"2016","journal-title":"Front. Microbiol."},{"key":"2026081410080497000_cit39","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1007\/978-1-4939-6685-1_13","article-title":"Natural Transformation\nof Oral Streptococci\nby Use of Synthetic Pheromones","volume":"1537","author":"Salvadori","year":"2017","journal-title":"Methods Mol.\nBiol."},{"issue":"8","key":"2026081410080497000_cit40","doi-asserted-by":"publisher","first-page":"2531","DOI":"10.1021\/bi702253n","article-title":"Substrate\nrecognition mechanism of the peptidase domain of the quorum-sensing-signal-producing\nABC transporter ComA from Streptococcus","volume":"47","author":"Kotake","year":"2008","journal-title":"Biochemistry"},{"issue":"8","key":"2026081410080497000_cit41","doi-asserted-by":"publisher","first-page":"4726","DOI":"10.1074\/jbc.M512516200","article-title":"Expression\nand characterization of\nthe peptidase domain of Streptococcus pneumoniae ComA, a bifunctional\nATP-binding cassette transporter involved in quorum sensing pathway","volume":"281","author":"Ishii","year":"2006","journal-title":"J. Biol. Chem."},{"issue":"5","key":"2026081410080497000_cit42","doi-asserted-by":"publisher","first-page":"1123","DOI":"10.1021\/acschembio.5c00180","article-title":"Elucidating the Role\nof the Competence Regulon Quorum Sensing Circuitry in Streptococcus cristatus","volume":"20","author":"Brennan","year":"2025","journal-title":"ACS Chem. Biol."},{"issue":"14","key":"2026081410080497000_cit43","doi-asserted-by":"publisher","first-page":"3104","DOI":"10.1021\/acs.biochem.5c00195","article-title":"Deciphering the Regulatory\nRole and Molecular Interactions\nThat Drive the Competence Regulon Quorum Sensing Circuitry in Streptococcus constellatus","volume":"64","author":"Mehrani","year":"2025","journal-title":"Biochemistry"},{"issue":"4","key":"2026081410080497000_cit44","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1021\/acschembio.7b00007","article-title":"Structure-Activity\nRelationships of the Competence\nStimulating Peptides (CSPs) in Streptococcus pneumoniae Reveal Motifs\nCritical for Intra-group and Cross-group ComD Receptor Activation","volume":"12","author":"Yang","year":"2017","journal-title":"ACS Chem. Biol."},{"issue":"5","key":"2026081410080497000_cit45","doi-asserted-by":"publisher","first-page":"1744","DOI":"10.1128\/JB.188.5.1744-1749.2006","article-title":"A hydrophobic\npatch in the competence-stimulating Peptide, a pneumococcal competence\npheromone, is essential for specificity and biological activity","volume":"188","author":"Johnsborg","year":"2006","journal-title":"J. Bacteriol."},{"issue":"12","key":"2026081410080497000_cit46","doi-asserted-by":"publisher","first-page":"3631","DOI":"10.1021\/acsinfecdis.5c00926","article-title":"The Quorum\nSensing-Controlled Competence Regulon Drives H2O2 Production in Streptococcus gordonii","volume":"11","author":"Brennan","year":"2025","journal-title":"ACS Infect.\nDis."},{"issue":"12","key":"2026081410080497000_cit47","doi-asserted-by":"publisher","first-page":"2834","DOI":"10.1021\/acschembio.1c00746","article-title":"Elucidating the Role and Structure\u2013Activity\nRelationships of the Streptococcus oligofermentans Competence-Stimulating\nPeptide","volume":"16","author":"Mull","year":"2021","journal-title":"ACS Chem. Biol."},{"issue":"4","key":"2026081410080497000_cit48","doi-asserted-by":"publisher","DOI":"10.1002\/pep2.24222","article-title":"Strategies to Attenuate\nthe Competence Regulon in Streptococcus\npneumoniae","volume":"113","author":"Lella","year":"2021","journal-title":"Pept. Sci."},{"issue":"8","key":"2026081410080497000_cit49","doi-asserted-by":"publisher","first-page":"1270","DOI":"10.1021\/acs.biochem.6c00057","article-title":"Mapping the\nStructural Determinants of Quorum Sensing Activity in Streptococcus\nsinensis Via Mutational Analysis of Its Competence Stimulating Peptide","volume":"65","author":"Renshaw","year":"2026","journal-title":"Biochemistry"}],"container-title":["ACS Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsinfecdis.6c00548","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/12\/8\/2863\/66020722\/acsinfecdis.6c00548.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/12\/8\/2863\/66020722\/acsinfecdis.6c00548.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T14:34:51Z","timestamp":1786718091000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article\/12\/8\/2863\/5215085\/Structure-Activity-Analysis-of-the-Competence"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,18]]},"references-count":49,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,7,18]]},"published-print":{"date-parts":[[2026,8,14]]}},"URL":"https:\/\/doi.org\/10.1021\/acsinfecdis.6c00548","relation":{},"ISSN":["2373-8227"],"issn-type":[{"value":"2373-8227","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,18]]}}}