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Improving our understanding of these defense pathways can reveal vulnerable points in Mtb pathogenesis. In this study, we combined genetic, structural, computational, biochemical, and biophysical approaches to identify a novel enzyme class represented by Rv2466c. We show that Rv2466c is a mycothiol-dependent nitroreductase of Mtb and can reduce the nitro group of a novel mycobactericidal compound using mycothiol as a cofactor. In addition to its function as a nitroreductase, Rv2466c confers partial protection to menadione stress.<\/jats:p>","DOI":"10.1021\/acsinfecdis.7b00111","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T13:54:44Z","timestamp":1519221284000},"page":"771-787","source":"Crossref","is-referenced-by-count":27,"title":["Identification of a Mycothiol-Dependent Nitroreductase\nfrom\n                    <i>Mycobacterium tuberculosis<\/i>"],"prefix":"10.1021","volume":"4","author":[{"given":"Ana","family":"Negri","sequence":"first","affiliation":[{"name":"Schro\u0308dinger, Inc. , 120 West 45th Street , , ,","place":["New York, New York, United States, 10036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prisca","family":"Javidnia","sequence":"additional","affiliation":[{"name":"Weill Cornell Medical College , , 1300 York Avenue , , ,","place":["New York, New York, United States, 10065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ran","family":"Mu","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, P. R. China, 100084"]},{"name":"Tsinghua University , , ,","place":["Beijing, P. R. China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, P. R. China, 100084"]},{"name":"Tsinghua University , , ,","place":["Beijing, P. R. 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David","family":"Warren","sequence":"additional","affiliation":[{"name":"Weill Cornell Medical College , , 1300 York Avenue , , ,","place":["New York, New York, United States, 10065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deena A.","family":"Oren","sequence":"additional","affiliation":[{"name":"Rockefeller University , , 1230 York Avenue , , ,","place":["New York, New York, United States, 10065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thijs","family":"Beuming","sequence":"additional","affiliation":[{"name":"Schro\u0308dinger, Inc. , 120 West 45th Street , , ,","place":["New York, New York, United States, 10036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyao","family":"Wang","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, P. R. China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Wu","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, P. R. China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haitao","family":"Li","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, P. R. China, 100084"]},{"name":"Tsinghua University , , ,","place":["Beijing, P. R. China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyu Y.","family":"Rhee","sequence":"additional","affiliation":[{"name":"Weill Cornell Medical College , , 1300 York Avenue , , ,","place":["New York, New York, United States, 10065"]},{"name":"Weill Cornell Medical College , , 1300 York Avenue , , ,","place":["New York, New York, United States, 10065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl F.","family":"Nathan","sequence":"additional","affiliation":[{"name":"Weill Cornell Medical College , , 1300 York Avenue , , ,","place":["New York, New York, United States, 10065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, P. R. China, 100084"]},{"name":"Tsinghua University , , ,","place":["Beijing, P. R. China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2000-2700","authenticated-orcid":true,"given":"Selin","family":"Somersan-Karakaya","sequence":"additional","affiliation":[{"name":"Weill Cornell Medical College , , 1300 York Avenue , , ,","place":["New York, New York, United States, 10065"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2018,2,21]]},"reference":[{"issue":"10","key":"2026090216325076100_cit1","doi-asserted-by":"publisher","first-page":"1176","DOI":"10.1038\/ng.2744","article-title":"Out-of-Africa migration and Neolithic coexpansion of\nMycobacterium tuberculosis with modern humans","volume":"45","author":"Comas","year":"2013","journal-title":"Nat. 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