{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T19:12:47Z","timestamp":1788289967852,"version":"build-2803163510"},"reference-count":54,"publisher":"American Chemical Society (ACS)","issue":"7","license":[{"start":{"date-parts":[[2019,5,29]],"date-time":"2019-05-29T00:00:00Z","timestamp":1559088000000},"content-version":"vor","delay-in-days":36,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["U01HL127522"],"award-info":[{"award-number":["U01HL127522"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272201100009I"],"award-info":[{"award-number":["HHSN272201100009I"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI134054"],"award-info":[{"award-number":["R01AI134054"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007259","name":"Stony Brook University","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100007259","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009115","name":"Center for Biotechnology, Stony Brook University","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100009115","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"New York State Department of Economic Development","doi-asserted-by":"publisher","award":["C14051"],"award-info":[{"award-number":["C14051"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Center for Advanced Technology, Stony Brook University","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Cold Spring Harbor Laboratory","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>One-third of the world\u2019s population carries Mycobacterium tuberculosis (Mtb), the infectious agent that causes tuberculosis (TB), and every 17 s someone dies of TB. After infection, Mtb can live dormant for decades in a granuloma structure arising from the host immune response, and cholesterol is important for this persistence of Mtb. Current treatments require long-duration drug regimens with many associated toxicities, which are compounded by the high doses required. We phenotypically screened 35 6-azasteroid analogues against Mtb and found that, at low micromolar concentrations, a subset of the analogues sensitized Mtb to multiple TB drugs. Two analogues were selected for further study to characterize the bactericidal activity of bedaquiline and isoniazid under normoxic and low-oxygen conditions. These two 6-azasteroids showed strong synergy with bedaquiline (fractional inhibitory concentration index = 0.21, bedaquiline minimal inhibitory concentration = 16 nM at 1 \u03bcM 6-azasteroid). The rate at which spontaneous resistance to one of the 6-azasteroids arose in the presence of bedaquiline was approximately 10\u20139, and the 6-azasteroid-resistant mutants retained their isoniazid and bedaquiline sensitivity. Genes in the cholesterol-regulated Mce3R regulon were required for 6-azasteroid activity, whereas genes in the cholesterol catabolism pathway were not. Expression of a subset of Mce3R genes was down-regulated upon 6-azasteroid treatment. The Mce3R regulon is implicated in stress resistance and is absent in saprophytic mycobacteria. This regulon encodes a cholesterol-regulated stress-resistance pathway that we conclude is important for pathogenesis and contributes to drug tolerance, and this pathway is vulnerable to small-molecule targeting in live mycobacteria.<\/jats:p>","DOI":"10.1021\/acsinfecdis.9b00099","type":"journal-article","created":{"date-parts":[[2019,4,23]],"date-time":"2019-04-23T07:45:41Z","timestamp":1556005541000},"page":"1239-1251","source":"Crossref","is-referenced-by-count":16,"title":["Mce3R Stress-Resistance Pathway Is Vulnerable to Small-Molecule\nTargeting That Improves Tuberculosis Drug Activities"],"prefix":"10.1021","volume":"5","author":[{"given":"Xinxin","family":"Yang","sequence":"first","affiliation":[{"name":"Stony Brook University , , 100 John S. Toll Drive , , ,","place":["Stony Brook, New York, United States, 11794-3400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4700-8654","authenticated-orcid":true,"given":"Tianao","family":"Yuan","sequence":"additional","affiliation":[{"name":"Stony Brook University , , 100 John S. Toll Drive , , ,","place":["Stony Brook, New York, United States, 11794-3400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Ma","sequence":"additional","affiliation":[{"name":"University of Illinois at Chicago , , 833 South Wood Street, 425 PHARM , , ,","place":["Chicago, Illinois, United States, 60612-7231"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kieran I.","family":"Chacko","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , ,","place":["New York City, New York, United States, 10029"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Smith","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , ,","place":["New York City, New York, United States, 10029"]},{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , , ,","place":["New York City, New York, United States, 10029-6574"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gintaras","family":"Deikus","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , ,","place":["New York City, New York, United States, 10029"]},{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , , ,","place":["New York City, New York, United States, 10029-6574"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Sebra","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , ,","place":["New York City, New York, United States, 10029"]},{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , , ,","place":["New York City, New York, United States, 10029-6574"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Kasarskis","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , ,","place":["New York City, New York, United States, 10029"]},{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , , ,","place":["New York City, New York, United States, 10029-6574"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harm","family":"van Bakel","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , ,","place":["New York City, New York, United States, 10029"]},{"name":"Icahn School of Medicine at Mount Sinai , , One Gustave L. Levy Place , Box 1498, , , ,","place":["New York City, New York, United States, 10029-6574"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8698-0243","authenticated-orcid":true,"given":"Scott G.","family":"Franzblau","sequence":"additional","affiliation":[{"name":"University of Illinois at Chicago , , 833 South Wood Street, 425 PHARM , , ,","place":["Chicago, Illinois, United States, 60612-7231"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2835-7760","authenticated-orcid":true,"given":"Nicole S.","family":"Sampson","sequence":"additional","affiliation":[{"name":"Stony Brook University , , 100 John S. Toll Drive , , ,","place":["Stony Brook, New York, United States, 11794-3400"]},{"name":"Stony Brook University , , 100 John S. Toll Drive , , ,","place":["Stony Brook, New York, United States, 11794-3400"]},{"name":"Wallenberg Research Centre at Stellenbosch University , , 10 Marais Street , ,","place":["Stellenbosch, South Africa, 7600"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2019,4,23]]},"reference":[{"issue":"11","key":"2026090114143403700_cit1","doi-asserted-by":"publisher","first-page":"4376","DOI":"10.1073\/pnas.0711159105","article-title":"Mycobacterial persistence requires the utilization\nof host cholesterol","volume":"105","author":"Pandey","year":"2008","journal-title":"Proc. Natl. Acad. Sci.\nU. S. A."},{"issue":"1","key":"2026090114143403700_cit2","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1128\/IAI.00893-09","article-title":"A thiolase of Mycobacterium tuberculosis is required for virulence and\nproduction of androstenedione and androstadienedione from cholesterol","volume":"78","author":"Nesbitt","year":"2010","journal-title":"Infect. Immun."},{"issue":"16","key":"2026090114143403700_cit3","doi-asserted-by":"publisher","first-page":"5232","DOI":"10.1128\/JB.00452-09","article-title":"igr Genes and Mycobacterium tuberculosis cholesterol metabolism","volume":"191","author":"Chang","year":"2009","journal-title":"J. Bacteriol."},{"issue":"3","key":"2026090114143403700_cit4","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1111\/j.1365-2958.2010.07243.x","article-title":"Mycobacterium tuberculosis CYP125A1, a steroid C27 monooxygenase\nthat detoxifies intracellularly generated cholest-4-en-3-one","volume":"77","author":"Ouellet","year":"2010","journal-title":"Mol. Microbiol."},{"issue":"11","key":"2026090114143403700_cit5","doi-asserted-by":"publisher","first-page":"e1000204","DOI":"10.1371\/journal.ppat.1000204","article-title":"Foamy macrophages from tuberculous\npatients\u2019 granulomas constitute a nutrient-rich reservoir for M. tuberculosis persistence","volume":"4","author":"Peyron","year":"2008","journal-title":"PLoS\nPathog."},{"issue":"2","key":"2026090114143403700_cit6","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1002\/path.1906","article-title":"New insights into the function of\ngranulomas in human tuberculosis","volume":"208","author":"Ulrichs","year":"2006","journal-title":"J. Pathol."},{"key":"2026090114143403700_cit7","doi-asserted-by":"publisher","first-page":"e1000344","DOI":"10.1371\/journal.ppat.1000344","article-title":"Studies of a ring-cleaving dioxygenase\nilluminate the role of cholesterol metabolism in the pathogenesis\nof Mycobacterium tuberculosis","volume":"5","author":"Yam","year":"2009","journal-title":"PLoS Pathog."},{"issue":"6","key":"2026090114143403700_cit8","doi-asserted-by":"publisher","first-page":"1947","DOI":"10.1073\/pnas.0605728104","article-title":"A gene\ncluster encoding cholesterol catabolism in a soil actinomycete provides\ninsight into Mycobacterium tuberculosis survival\nin macrophages","volume":"104","author":"Van der Geize","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S.\nA."},{"issue":"4","key":"2026090114143403700_cit9","doi-asserted-by":"publisher","first-page":"269","DOI":"10.3109\/10409238.2014.895700","article-title":"Pathogen roid rage: cholesterol utilization\nby Mycobacterium tuberculosis","volume":"49","author":"Wipperman","year":"2014","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"issue":"Pt 5","key":"2026090114143403700_cit10","doi-asserted-by":"publisher","first-page":"1362","DOI":"10.1099\/mic.0.034538-0","article-title":"Cholesterol utilization in mycobacteria\nis controlled by two TetR-type transcriptional regulators: kstR and kstR2","volume":"156","author":"Kendall","year":"2010","journal-title":"Microbiology"},{"issue":"3","key":"2026090114143403700_cit11","doi-asserted-by":"publisher","first-page":"684","DOI":"10.1111\/j.1365-2958.2007.05827.x","article-title":"A highly conserved\ntranscriptional repressor controls a large regulon involved in lipid\ndegradation in Mycobacterium smegmatis and Mycobacterium tuberculosis","volume":"65","author":"Kendall","year":"2007","journal-title":"Mol. Microbiol."},{"issue":"25","key":"2026090114143403700_cit12","doi-asserted-by":"publisher","first-page":"17576","DOI":"10.1074\/jbc.M113.545715","article-title":"Deciphering the\ntranscriptional regulation of cholesterol catabolic pathway in mycobacteria:\nidentification of the inducer of KstR repressor","volume":"289","author":"Garcia-Fernandez","year":"2014","journal-title":"J. Biol. Chem."},{"issue":"2","key":"2026090114143403700_cit13","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1074\/jbc.M114.607481","article-title":"Structural and functional\ncharacterization of a ketosteroid transcriptional regulator of Mycobacterium tuberculosis","volume":"290","author":"Crowe","year":"2015","journal-title":"J. Biol.\nChem."},{"issue":"4","key":"2026090114143403700_cit14","doi-asserted-by":"publisher","first-page":"1887","DOI":"10.1021\/acs.chemrev.7b00602","article-title":"Hit generation in TB drug discovery: from genome to\ngranuloma","volume":"118","author":"Yuan","year":"2018","journal-title":"Chem. Rev."},{"issue":"5","key":"2026090114143403700_cit15","doi-asserted-by":"publisher","first-page":"405","DOI":"10.2174\/156802606776743101","article-title":"Discovery and clinical development\nof dutasteride, a potent dual 5alpha-reductase inhibitor","volume":"6","author":"Frye","year":"2006","journal-title":"Curr. Top. Med. Chem."},{"issue":"14","key":"2026090114143403700_cit16","doi-asserted-by":"publisher","first-page":"2621","DOI":"10.1021\/jm00014a015","article-title":"Structure-activity relationships\nfor inhibition of type 1 and 2 human 5 alpha-reductase and human adrenal\n3 beta-hydroxy-delta 5-steroid dehydrogenase\/3-keto-delta 5-steroid\nisomerase by 6-azaandrost-4-en-3-ones: optimization of the C17 substituent","volume":"38","author":"Frye","year":"1995","journal-title":"J. Med. Chem."},{"issue":"26","key":"2026090114143403700_cit17","doi-asserted-by":"publisher","first-page":"4313","DOI":"10.1021\/jm00078a022","article-title":"6-Azasteroids:\npotent dual inhibitors of human type 1 and 2 steroid 5 alpha-reductase","volume":"36","author":"Frye","year":"1993","journal-title":"J. Med. Chem."},{"issue":"15","key":"2026090114143403700_cit18","doi-asserted-by":"publisher","first-page":"2352","DOI":"10.1021\/jm00041a014","article-title":"6-Azasteroids: structure-activity relationships for\ninhibition of type 1 and 2 human 5 alpha-reductase and human adrenal\n3 beta-hydroxy-delta 5-steroid dehydrogenase\/3-keto-delta 5-steroid\nisomerase","volume":"37","author":"Frye","year":"1994","journal-title":"J. Med. Chem."},{"issue":"8","key":"2026090114143403700_cit19","doi-asserted-by":"publisher","first-page":"2216","DOI":"10.1016\/j.bmcl.2011.03.004","article-title":"Inhibition\nof the M. tuberculosis 3beta-hydroxysteroid dehydrogenase\nby azasteroids","volume":"21","author":"Thomas","year":"2011","journal-title":"Bioorg. Med. Chem. Lett."},{"issue":"6","key":"2026090114143403700_cit20","doi-asserted-by":"publisher","first-page":"1473","DOI":"10.1128\/JB.01210-10","article-title":"Cholesterol is not\nan essential source of nutrition for Mycobacterium tuberculosis during infection","volume":"193","author":"Yang","year":"2011","journal-title":"J. Bacteriol."},{"issue":"31","key":"2026090114143403700_cit21","doi-asserted-by":"publisher","first-page":"9058","DOI":"10.1021\/bi700688x","article-title":"Rv1106c from Mycobacterium tuberculosis is a 3\u03b2-hydroxysteroid\ndehydrogenase","volume":"46","author":"Yang","year":"2007","journal-title":"Biochemistry"},{"issue":"19","key":"2026090114143403700_cit22","doi-asserted-by":"publisher","first-page":"4331","DOI":"10.1128\/JB.00502-13","article-title":"Shrinking the FadE proteome of Mycobacterium tuberculosis: insights into cholesterol metabolism through\nidentification of an \u03b12\u03b22 heterotetrameric\nacyl coenzyme A dehydrogenase family","volume":"195","author":"Wipperman","year":"2013","journal-title":"J. Bacteriol."},{"issue":"12","key":"2026090114143403700_cit23","doi-asserted-by":"publisher","first-page":"6902","DOI":"10.1128\/IAI.72.12.6902-6913.2004","article-title":"Identification of\ntwo Mycobacterium marinum loci that affect interactions\nwith macrophages","volume":"72","author":"El-Etr","year":"2004","journal-title":"Infect. Immun."},{"key":"2026090114143403700_cit24","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1186\/1471-2180-7-4","article-title":"The Mycobacterium marinum mel2 locus displays similarity to bacterial bioluminescence\nsystems and plays a role in defense against reactive oxygen and nitrogen\nspecies","volume":"7","author":"Subbian","year":"2007","journal-title":"BMC Microbiol."},{"issue":"Suppl","key":"2026090114143403700_cit25","doi-asserted-by":"publisher","first-page":"S83","DOI":"10.1016\/S1472-9792(13)70016-7","article-title":"Molecular analysis of the Mycobacterium tuberculosis lux-like mel2 operon","volume":"93","author":"Janagama","year":"2013","journal-title":"Tuberculosis"},{"key":"2026090114143403700_cit26","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1186\/1471-2180-8-38","article-title":"Study of the role of Mce3R on the\ntranscription of mce genes of Mycobacterium tuberculosis","volume":"8","author":"Santangelo","year":"2008","journal-title":"BMC Microbiol."},{"issue":"Pt 7","key":"2026090114143403700_cit27","doi-asserted-by":"publisher","first-page":"2245","DOI":"10.1099\/mic.0.027086-0","article-title":"Mce3R, a TetR-type transcriptional\nrepressor, controls the expression of a regulon involved in lipid\nmetabolism in Mycobacterium tuberculosis","volume":"155","author":"de la Paz Santangelo","year":"2009","journal-title":"Microbiology"},{"key":"2026090114143403700_cit28","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1186\/s12864-015-1696-9","article-title":"Global analyses of TetR family transcriptional\nregulators in mycobacteria indicates conservation across species and\ndiversity in regulated functions","volume":"16","author":"Balhana","year":"2015","journal-title":"BMC Genomics"},{"issue":"3","key":"2026090114143403700_cit29","doi-asserted-by":"publisher","first-page":"1022","DOI":"10.1128\/AAC.01611-09","article-title":"Rates and\nmechanisms of resistance development in Mycobacterium tuberculosis to a novel diarylquinoline ATP synthase inhibitor","volume":"54","author":"Huitric","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"issue":"Pt 1","key":"2026090114143403700_cit30","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1099\/mic.0.042549-0","article-title":"Identification\nof novel diphenyl urea inhibitors of Mt-GuaB2 active against Mycobacterium tuberculosis","volume":"157","author":"Usha","year":"2011","journal-title":"Microbiology"},{"key":"2026090114143403700_cit31","doi-asserted-by":"publisher","first-page":"498395","DOI":"10.1155\/2014\/498395","article-title":"Cholesterol oxidase binds TLR2 and\nmodulates functional responses of human macrophages","volume":"2014","author":"Bednarska","year":"2014","journal-title":"Mediators Inflammation"},{"issue":"8","key":"2026090114143403700_cit32","doi-asserted-by":"publisher","first-page":"16078","DOI":"10.1038\/nmicrobiol.2016.78","article-title":"Network analysis identifies Rv0324\nand Rv0880 as regulators of bedaquiline tolerance in Mycobacterium tuberculosis","volume":"1","author":"Peterson","year":"2016","journal-title":"Nat. Microbiol."},{"issue":"1","key":"2026090114143403700_cit33","doi-asserted-by":"publisher","first-page":"3","DOI":"10.4161\/viru.22329","article-title":"Virulence factors of the Mycobacterium tuberculosis complex","volume":"4","author":"Forrellad","year":"2013","journal-title":"Virulence"},{"issue":"9","key":"2026090114143403700_cit34","doi-asserted-by":"publisher","first-page":"e1002251","DOI":"10.1371\/journal.ppat.1002251","article-title":"High-resolution phenotypic profiling defines genes\nessential for mycobacterial growth and cholesterol catabolism","volume":"7","author":"Griffin","year":"2011","journal-title":"PLoS Pathog."},{"issue":"6","key":"2026090114143403700_cit35","doi-asserted-by":"publisher","first-page":"2557","DOI":"10.1128\/IAI.01481-08","article-title":"Protection of Mycobacterium tuberculosis from reactive oxygen species\nconferred by the mel2 locus impacts persistence and\ndissemination","volume":"77","author":"Cirillo","year":"2009","journal-title":"Infect. Immun."},{"issue":"3","key":"2026090114143403700_cit36","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1016\/j.celrep.2015.12.056","article-title":"Ergothioneine maintains redox and bioenergetic homeostasis\nessential for drug susceptibility and virulence of Mycobacterium tuberculosis","volume":"14","author":"Saini","year":"2016","journal-title":"Cell Rep."},{"issue":"9","key":"2026090114143403700_cit37","doi-asserted-by":"publisher","first-page":"2505","DOI":"10.1128\/IAI.00072-16","article-title":"Transcriptional\nprofiling of Mycobacterium tuberculosis exposed to in vitro lysosomal stress","volume":"84","author":"Lin","year":"2016","journal-title":"Infect.\nImmun."},{"issue":"17","key":"2026090114143403700_cit38","doi-asserted-by":"publisher","first-page":"4495","DOI":"10.1073\/pnas.1704376114","article-title":"Enhanced respiration\nprevents drug tolerance and drug resistance in Mycobacterium tuberculosis","volume":"114","author":"Vilche\u0300ze","year":"2017","journal-title":"Proc. Natl. Acad. Sci.\nU. S. A."},{"issue":"14","key":"2026090114143403700_cit39","doi-asserted-by":"publisher","first-page":"3645","DOI":"10.1128\/JB.00166-10","article-title":"Variation\namong genome sequences of H37Rv strains of Mycobacterium tuberculosis from multiple laboratories","volume":"192","author":"Ioerger","year":"2010","journal-title":"J. Bacteriol."},{"issue":"10","key":"2026090114143403700_cit40","doi-asserted-by":"publisher","first-page":"1185","DOI":"10.1164\/rccm.201408-1502OC","article-title":"The DosR regulon modulates adaptive immunity and is\nessential for Mycobacterium tuberculosis persistence","volume":"191","author":"Mehra","year":"2015","journal-title":"Am. J. Respir. Crit. Care Med."},{"issue":"2","key":"2026090114143403700_cit41","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1128\/JB.01273-07","article-title":"Roles of SigB and SigF in the Mycobacterium tuberculosis sigma factor network","volume":"190","author":"Lee","year":"2008","journal-title":"J. Bacteriol."},{"issue":"6797","key":"2026090114143403700_cit42","doi-asserted-by":"publisher","first-page":"735","DOI":"10.1038\/35021074","article-title":"Persistence of Mycobacterium tuberculosis in macrophages and mice requires\nthe glyoxylate shunt enzyme isocitrate lyase","volume":"406","author":"McKinney","year":"2000","journal-title":"Nature"},{"issue":"2","key":"2026090114143403700_cit43","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1038\/nchembio.2259","article-title":"Inhibitors of Mycobacterium tuberculosis DosRST signaling and persistence","volume":"13","author":"Zheng","year":"2017","journal-title":"Nat. Chem. Biol."},{"key":"2026090114143403700_cit44","doi-asserted-by":"publisher","first-page":"13985","DOI":"10.1038\/srep13985","article-title":"In vitro and in vivo evaluation of synergism between anti-tubercular spectinamides and\nnon-classical tuberculosis antibiotics","volume":"5","author":"Bruhn","year":"2015","journal-title":"Sci.\nRep."},{"issue":"8","key":"2026090114143403700_cit45","doi-asserted-by":"publisher","first-page":"3861","DOI":"10.1128\/AAC.00474-11","article-title":"Synergistic drug combinations for\ntuberculosis therapy identified by a novel high-throughput screen","volume":"55","author":"Ramon-Garcia","year":"2011","journal-title":"Antimicrob. 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