{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T11:28:54Z","timestamp":1787052534610,"version":"build-2736575974"},"reference-count":36,"publisher":"American Chemical Society (ACS)","issue":"5","license":[{"start":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T00:00:00Z","timestamp":1568160000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T00:00:00Z","timestamp":1568160000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T00:00:00Z","timestamp":1568160000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2016YFD0501310"],"award-info":[{"award-number":["2016YFD0501310"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2018YFA0903400"],"award-info":[{"award-number":["2018YFA0903400"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2019M651984"],"award-info":[{"award-number":["2019M651984"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Decreasing the therapeutic pipeline for vancomycin-resistant Enterococci (VRE) calls for novel strategies to enhance our antibacterial arsenal. Herein, we investigated the potential applications of surface localized antimicrobial display (SLAY)-derived cationic peptides in the fight against VanA operon mediated vancomycin-resistant Enterococcus. Through determining their antibacterial spectrum, we found that SLAY peptide 1\/2 displayed moderate bactericidal activity against Enterococcus with minimal inhibitory concentration (MIC) values of 2\u20138 \u03bcg\/mL. Furthermore, we observed a significant synergistic activity between SLAY-P1 and vancomycin against VRE. Mechanistic studies demonstrated that SLAY-P1 specifically inhibits transcription of the vanRS two-component system, thereby restoring vancomycin activity and resulting in the accumulation of the cell wall precursor. Meaningfully, the combination of SLAY-P1 and vancomycin prevents the emergence of vancomycin resistance. Consistent with in vitro synergistic results, the addition of SLAY-P1 significantly enhanced the survival rates of Galleria mellonella larvae compared with vancomycin monotherapy. Taken together, these results suggested that SLAY-derived cationic peptides not only display antibacterial activity against VRE but also reverse vancomycin resistance in Enterococcus, providing promising candidates for combating vancomycin-resistant pathogens.<\/jats:p>","DOI":"10.1021\/acsinfecdis.9b00164","type":"journal-article","created":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T05:01:52Z","timestamp":1568178112000},"page":"761-767","source":"Crossref","is-referenced-by-count":29,"title":["Antagonizing Vancomycin Resistance in Enterococcus\nby Surface Localized Antimicrobial Display-Derived Peptides"],"prefix":"10.1021","volume":"6","author":[{"given":"Yuan","family":"Liu","sequence":"first","affiliation":[{"name":"Yangzhou University , , No. 88 University South Road , , ,","place":["Yangzhou, Jiangsu, China, 225009"]},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , No. 88 University South Road , , ,","place":["Yangzhou, Jiangsu, China, 225009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqian","family":"Jia","sequence":"additional","affiliation":[{"name":"Yangzhou University , , No. 88 University South Road , , ,","place":["Yangzhou, Jiangsu, China, 225009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kangni","family":"Yang","sequence":"additional","affiliation":[{"name":"Yangzhou University , , No. 88 University South Road , , ,","place":["Yangzhou, Jiangsu, China, 225009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruichao","family":"Li","sequence":"additional","affiliation":[{"name":"Yangzhou University , , No. 88 University South Road , , ,","place":["Yangzhou, Jiangsu, China, 225009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Xiao","sequence":"additional","affiliation":[{"name":"Yangzhou University , , No. 88 University South Road , , ,","place":["Yangzhou, Jiangsu, China, 225009"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6486-9600","authenticated-orcid":true,"given":"Zhiqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Yangzhou University , , No. 88 University South Road , , ,","place":["Yangzhou, Jiangsu, China, 225009"]},{"name":"Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses , No. 88 University South Road , , ,","place":["Yangzhou, Jiangsu, China, 225009"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2019,9,11]]},"reference":[{"key":"2026081806132745900_cit1","doi-asserted-by":"publisher","first-page":"11952","DOI":"10.1021\/acs.chemrev.6b00820","article-title":"Total syntheses of vancomycin-related\nglycopeptide antibiotics and key analogues","volume":"117","author":"Okano","year":"2017","journal-title":"Chem. 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Agents Chemother."},{"key":"2026081806132745900_cit30","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1038\/s41467-018-02875-z","article-title":"Overcoming mcr-1 mediated colistin resistance with colistin in combination\nwith other antibiotics","volume":"9","author":"Macnair","year":"2018","journal-title":"Nat. Commun."},{"key":"2026081806132745900_cit31","doi-asserted-by":"publisher","first-page":"13517","DOI":"10.1073\/pnas.1821410116","article-title":"Low cationicity is important for\nsystemic in vivo efficacy of database-derived peptides\nagainst drug-resistant Gram-positive pathogens","author":"Mishra","year":"2019","journal-title":"Proc. Natl. Acad. Sci. 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Ed."},{"key":"2026081806132745900_cit35","volume-title":"Performance\nStandards for Antimicrobial Susceptibility Testing","author":"Clinical and Laboratory Standards\nInstitute","year":"2015"},{"key":"2026081806132745900_cit36","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.1126\/science.1185723","article-title":"Plectasin, a fungal defensin, targets the bacterial cell wall precursor\nLipid II","volume":"328","author":"Schneider","year":"2010","journal-title":"Science"}],"container-title":["ACS Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsinfecdis.9b00164","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/6\/5\/761\/12727222\/id9b00164.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/6\/5\/761\/12727222\/id9b00164.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T10:55:44Z","timestamp":1787050544000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article\/6\/5\/761\/1292822\/Antagonizing-Vancomycin-Resistance-in-Enterococcus"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,11]]},"references-count":36,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2019,9,11]]},"published-print":{"date-parts":[[2020,5,8]]}},"URL":"https:\/\/doi.org\/10.1021\/acsinfecdis.9b00164","relation":{},"ISSN":["2373-8227"],"issn-type":[{"value":"2373-8227","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,11]]}}}