{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:17:40Z","timestamp":1788383860553,"version":"build-2803163510"},"reference-count":44,"publisher":"American Chemical Society (ACS)","issue":"2","license":[{"start":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T00:00:00Z","timestamp":1591920000000},"content-version":"vor","delay-in-days":212,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000052","name":"NIH Office of the Director","doi-asserted-by":"publisher","award":["S10OD018445"],"award-info":[{"award-number":["S10OD018445"]}],"id":[{"id":"10.13039\/100000052","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103429"],"award-info":[{"award-number":["P20GM103429"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20-GM103625"],"award-info":[{"award-number":["P20-GM103625"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM121293"],"award-info":[{"award-number":["P20GM121293"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R01GM118760"],"award-info":[{"award-number":["R01GM118760"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI119380"],"award-info":[{"award-number":["R01AI119380"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"publisher","award":["R01AR066050"],"award-info":[{"award-number":["R01AR066050"]}],"id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000090","name":"Congressionally Directed Medical Research Programs","doi-asserted-by":"publisher","award":["OR140356"],"award-info":[{"award-number":["OR140356"]}],"id":[{"id":"10.13039\/100000090","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000090","name":"Congressionally Directed Medical Research Programs","doi-asserted-by":"publisher","award":["W81XWH-15-1-0716"],"award-info":[{"award-number":["W81XWH-15-1-0716"]}],"id":[{"id":"10.13039\/100000090","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR000039"],"award-info":[{"award-number":["UL1TR000039"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008480","name":"Arkansas Research Alliance","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100008480","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,2,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We used a murine model of postsurgical osteomyelitis (OM) to evaluate the relative virulence of the Staphylococcus aureus strain LAC and five isogenic variants that differ in the functional status of saeRS and sarA relative to each other. LAC and a variant in which saeRS activity is increased (saeC) were comparably virulent to each other, while \u0394saeRS, \u0394sarA, \u0394saeRS\/\u0394sarA, and saeC\/\u0394sarA mutants were all attenuated to a comparable degree. Phenotypic comparisons including a mass-based proteomics approach that allowed us to assess the number and abundance of full-length proteins suggested that mutation of saeRS attenuates virulence in our OM model owing primarily to the decreased production of S.\u00a0aureus virulence factors, while mutation of sarA does so owing to protease-mediated degradation of these same virulence factors. This was confirmed by demonstrating that eliminating protease production restored virulence to a greater extent in a LAC sarA mutant than in the isogenic saeRS mutant. Irrespective of the mechanism involved, mutation of saeRS or sarA was shown to result in reduced accumulation of virulence factors of potential importance. Thus, using our proteomics approach we correlated the abundance of specific proteins with virulence in these six strains and identified 14 proteins that were present in a significantly increased amount (log2 \u2265 5.0) in both virulent strains by comparison to all four attenuated strains. We examined biofilm formation and virulence in our OM model using a LAC mutant unable to produce one of these 14 proteins, specifically staphylocoagulase. The results confirmed that mutation of coa limits biofilm formation and, to a lesser extent, virulence in our OM model, although in both cases the limitation was reduced by comparison to the isogenic sarA mutant.<\/jats:p>","DOI":"10.1021\/acsinfecdis.9b00291","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T20:09:49Z","timestamp":1573675789000},"page":"237-249","source":"Crossref","is-referenced-by-count":25,"title":["Exploiting Correlations\nbetween Protein Abundance and the Functional Status of\n                    <i>saeRS<\/i>\n                    and\n                    <i>sarA<\/i>\n                    To Identify Virulence Factors of Potential\nImportance in the Pathogenesis of\n                    <i>Staphylococcus aureus<\/i>\n                    Osteomyelitis"],"prefix":"10.1021","volume":"6","author":[{"given":"Aura M.","family":"Ramirez","sequence":"first","affiliation":[{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street, Slot 511 , , ,","place":["Little Rock, Arkansas, United States, 72205"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie D.","family":"Byrum","sequence":"additional","affiliation":[{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street, Slot 516 , , ,","place":["Little Rock, Arkansas, United States, 72205"]},{"name":"Arkansas Children\u2019s Research Institute , 1 Children\u2019s Way , , ,","place":["Little Rock, Arkansas, United States, 72202"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen E.","family":"Beenken","sequence":"additional","affiliation":[{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street, Slot 511 , , ,","place":["Little Rock, Arkansas, United States, 72205"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charity","family":"Washam","sequence":"additional","affiliation":[{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street, Slot 516 , , ,","place":["Little Rock, Arkansas, United States, 72205"]},{"name":"Arkansas Children\u2019s Research Institute , 1 Children\u2019s Way , , ,","place":["Little Rock, Arkansas, United States, 72202"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rick D.","family":"Edmondson","sequence":"additional","affiliation":[{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street, Slot 516 , , ,","place":["Little Rock, Arkansas, United States, 72205"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel G.","family":"Mackintosh","sequence":"additional","affiliation":[{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street, Slot 516 , , ,","place":["Little Rock, Arkansas, United States, 72205"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horace J.","family":"Spencer","sequence":"additional","affiliation":[{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street , , ,","place":["Little Rock, Arkansas, United States, 72205"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3672-4460","authenticated-orcid":true,"given":"Alan J.","family":"Tackett","sequence":"additional","affiliation":[{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street, Slot 516 , , ,","place":["Little Rock, Arkansas, United States, 72205"]},{"name":"Arkansas Children\u2019s Research Institute , 1 Children\u2019s Way , , ,","place":["Little Rock, Arkansas, United States, 72202"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0878-0692","authenticated-orcid":true,"given":"Mark S.","family":"Smeltzer","sequence":"additional","affiliation":[{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street, Slot 511 , , ,","place":["Little Rock, Arkansas, United States, 72205"]},{"name":"University of Arkansas for Medical Sciences , , 4301 W. Markham Street, Slot 531 , , ,","place":["Little Rock, Arkansas, United States, 72205"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2019,11,13]]},"reference":[{"key":"2026090216340148700_cit1","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1007\/82_2016_3","article-title":"Worldwide\nEpidemiology and Antibiotic Resistance of Staphylococcus aureus","volume":"409","author":"Monaco","year":"2016","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"2026090216340148700_cit2","doi-asserted-by":"publisher","first-page":"153","DOI":"10.2174\/1874325001812010153","article-title":"Chronic Osteomyelitis\n- Bacterial Flora, Antibiotic Sensitivity and Treatment Challenges","volume":"12","author":"Jerzy","year":"2018","journal-title":"Open Orthop J."},{"issue":"11","key":"2026090216340148700_cit3","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1038\/nrmicro2880","article-title":"From genotype to\nphenotype: can systems biology be used to predict Staphylococcus aureus\nvirulence?","volume":"10","author":"Priest","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"issue":"9","key":"2026090216340148700_cit4","doi-asserted-by":"publisher","first-page":"2586","DOI":"10.1128\/IAI.00152-16","article-title":"Impact\nof sarA and Phenol-Soluble Modulins on the Pathogenesis of Osteomyelitis\nin Diverse Clinical Isolates of Staphylococcus aureus","volume":"84","author":"Loughran","year":"2016","journal-title":"Infect. Immun."},{"issue":"6","key":"2026090216340148700_cit5","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1016\/j.chom.2013.05.003","article-title":"A secreted bacterial protease tailors\nthe Staphylococcus aureus virulence repertoire to modulate bone remodeling\nduring osteomyelitis","volume":"13","author":"Cassat","year":"2013","journal-title":"Cell Host Microbe"},{"issue":"6","key":"2026090216340148700_cit6","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1111\/mmi.12629","article-title":"Impact\nof the functional status of saeRS on in vivo phenotypes of Staphylococcus\naureus sarA mutants","volume":"92","author":"Beenken","year":"2014","journal-title":"Mol. Microbiol."},{"issue":"6","key":"2026090216340148700_cit7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038453","article-title":"saeRS and sarA act synergistically\nto repress protease production and promote biofilm formation in Staphylococcus\naureus","volume":"7","author":"Mrak","year":"2012","journal-title":"PLoS One"},{"issue":"12","key":"2026090216340148700_cit8","doi-asserted-by":"publisher","first-page":"2948","DOI":"10.1128\/JB.01517-10","article-title":"Defining the strain-dependent\nimpact of the Staphylococcal accessory regulator (sarA) on the alpha-toxin\nphenotype of Staphylococcus aureus","volume":"193","author":"Zielinska","year":"2011","journal-title":"J. Bacteriol."},{"issue":"5","key":"2026090216340148700_cit9","doi-asserted-by":"publisher","first-page":"1183","DOI":"10.1111\/mmi.12048","article-title":"sarA-mediated repression\nof protease production plays a key role in the pathogenesis of Staphylococcus\naureus USA300 isolates","volume":"86","author":"Zielinska","year":"2012","journal-title":"Mol. Microbiol."},{"key":"2026090216340148700_cit10","doi-asserted-by":"crossref","unstructured":"Gimza, B., Larias, M., Budny, B., and Shaw, L. (2019) Mapping\nthe global network of extracellular protease regulation in Staphylococcus\naureus.  bioRxiv10.1101\/764423.","DOI":"10.1101\/764423"},{"key":"2026090216340148700_cit11","doi-asserted-by":"publisher","first-page":"3384","DOI":"10.1021\/acs.jproteome.8b00288","article-title":"Label-Free Proteomic\nApproach to Characterize Protease-Dependent and -Independent Effects\nof sarA Inactivation on the Staphylococcus aureus Exoproteome","volume":"17","author":"Byrum","year":"2018","journal-title":"J. Proteome Res."},{"key":"2026090216340148700_cit12","unstructured":"Oliveros, J.\n           (2007)  Venny:\nAn Interactive Tool for Comparing Lists with Venn\u2019s Diagrams."},{"issue":"10","key":"2026090216340148700_cit13","doi-asserted-by":"publisher","first-page":"4288","DOI":"10.1093\/nar\/gks042","article-title":"Differential\nexpression analysis of multifactor RNA-Seq experiments with respect\nto biological variation","volume":"40","author":"McCarthy","year":"2012","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"2026090216340148700_cit14","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","article-title":"edgeR: a Bioconductor package for differential expression\nanalysis of digital gene expression data","volume":"26","author":"Robinson","year":"2010","journal-title":"Bioinformatics"},{"issue":"1","key":"2026090216340148700_cit15","doi-asserted-by":"publisher","first-page":"e00537","DOI":"10.1128\/mBio.00537-12","article-title":"A genetic resource for rapid and comprehensive phenotype\nscreening of nonessential Staphylococcus aureus genes","volume":"4","author":"Fey","year":"2013","journal-title":"mBio"},{"issue":"12","key":"2026090216340148700_cit16","doi-asserted-by":"publisher","first-page":"1883","DOI":"10.1093\/infdis\/jiv319","article-title":"An Essential Role for Coagulase in Staphylococcus aureus\nBiofilm Development Reveals New Therapeutic Possibilities for Device-Related\nInfections","volume":"212","author":"Zapotoczna","year":"2015","journal-title":"J. Infect. Dis."},{"issue":"2","key":"2026090216340148700_cit17","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1159\/000354440","article-title":"Staphylococcal protein\nA, Panton-Valentine leukocidin and coagulase aggravate the bone loss\nand bone destruction in osteomyelitis","volume":"32","author":"Jin","year":"2013","journal-title":"Cell.\nPhysiol. Biochem."},{"key":"2026090216340148700_cit18","doi-asserted-by":"publisher","first-page":"190S","DOI":"10.1097\/PRS.0b013e3182025070","article-title":"Surgical treatment of osteomyelitis","volume":"127","author":"Cierny","year":"2011","journal-title":"Plast. Reconstr. Surg."},{"key":"2026090216340148700_cit19","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.arth.2012.02.022","article-title":"Economic burden of periprosthetic joint infection in\nthe United States","volume":"27","author":"Kurtz","year":"2012","journal-title":"J. Arthroplasty"},{"issue":"9431","key":"2026090216340148700_cit20","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1016\/S0140-6736(04)16727-5","article-title":"Osteomyelitis","volume":"364","author":"Lew","year":"2004","journal-title":"Lancet"},{"issue":"8","key":"2026090216340148700_cit21","doi-asserted-by":"publisher","first-page":"1038","DOI":"10.1016\/j.ijmm.2014.07.013","article-title":"Staphylococcus\naureus isolates from chronic osteomyelitis are characterized by high\nhost cell invasion and intracellular adaptation, but still induce\ninflammation","volume":"304","author":"Kalinka","year":"2014","journal-title":"Int. J. Med. Microbiol."},{"key":"2026090216340148700_cit22","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01775-14","article-title":"High-resolution transcriptomic analysis of the adaptive\nresponse of Staphylococcus aureus during acute and chronic phases\nof osteomyelitis","author":"Szafranska","year":"2014","journal-title":"mBio"},{"issue":"5","key":"2026090216340148700_cit23","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010790","article-title":"Epistatic relationships between sarA and agr in Staphylococcus\naureus biofilm formation","volume":"5","author":"Beenken","year":"2010","journal-title":"PLoS One"},{"issue":"1","key":"2026090216340148700_cit24","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1099\/mic.0.063016-0","article-title":"Staphylococcus aureus\nprotein A binding to osteoblast tumour necrosis factor receptor 1\nresults in activation of nuclear factor kappa B and release of interleukin-6\nin bone infection","volume":"159","author":"Claro","year":"2013","journal-title":"Microbiology"},{"issue":"4","key":"2026090216340148700_cit25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0018748","article-title":"Staphylococcus aureus\nprotein A binds to osteoblasts and triggers signals that weaken bone\nin osteomyelitis","volume":"6","author":"Claro","year":"2011","journal-title":"PLoS One"},{"issue":"10","key":"2026090216340148700_cit26","doi-asserted-by":"publisher","first-page":"1975","DOI":"10.1016\/j.bbadis.2016.07.016","article-title":"Staphylococcus aureus protein A enhances\nosteoclastogenesis via TNFR1 and EGFR signaling","volume":"1862","author":"Mendoza Bertelli","year":"2016","journal-title":"Biochim. Biophys. Acta, Mol. Basis Dis."},{"issue":"5","key":"2026090216340148700_cit27","doi-asserted-by":"publisher","first-page":"6020","DOI":"10.3892\/mmr.2017.7316","article-title":"Staphylococcus aureus\nProtein A induces osteoclastogenesis via the NFkappaB signaling pathway","volume":"16","author":"Ren","year":"2017","journal-title":"Mol. Med. Rep."},{"issue":"7","key":"2026090216340148700_cit28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040586","article-title":"Staphylococcus aureus protein A plays\na critical role in mediating bone destruction and bone loss in osteomyelitis","volume":"7","author":"Widaa","year":"2012","journal-title":"PLoS One"},{"issue":"1","key":"2026090216340148700_cit29","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1111\/j.1574-695X.2007.00357.x","article-title":"Osteomyelitis and the role of biofilms in chronic infection","volume":"52","author":"Brady","year":"2008","journal-title":"FEMS Immunol. Med. Microbiol."},{"issue":"11","key":"2026090216340148700_cit30","doi-asserted-by":"publisher","first-page":"3835","DOI":"10.1128\/JB.00167-08","article-title":"A novel Staphylococcus aureus biofilm phenotype mediated\nby the fibronectin-binding proteins","volume":"190","author":"O\u2019Neill","year":"2008","journal-title":"FnBPA and\nFnBPB. J. Bacteriol"},{"key":"2026090216340148700_cit31","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1097\/00003086-200101000-00032","article-title":"Collagen and\nfibronectin binding in experimental staphylococcal osteomyelitis","volume":"382","author":"Johansson","year":"2001","journal-title":"Clin. Orthop. Relat. Res."},{"issue":"12","key":"2026090216340148700_cit32","doi-asserted-by":"publisher","first-page":"5062","DOI":"10.1128\/IAI.00516-10","article-title":"Listeria monocytogenes\nuses Listeria adhesion protein (LAP) to promote bacterial transepithelial\ntranslocation and induces expression of LAP receptor Hsp60","volume":"78","author":"Burkholder","year":"2010","journal-title":"Infect. Immun."},{"issue":"2","key":"2026090216340148700_cit33","doi-asserted-by":"publisher","first-page":"254","DOI":"10.1111\/j.1365-2249.2006.03047.x","article-title":"Pneumococcal 6-phosphogluconate-dehydrogenase, a putative\nadhesin, induces protective immune response in mice","volume":"144","author":"Daniely","year":"2006","journal-title":"Clin. Exp. Immunol."},{"issue":"1","key":"2026090216340148700_cit34","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/j.jmb.2005.04.063","article-title":"Group A streptococcal surface GAPDH,\nSDH, recognizes uPAR\/CD87 as its receptor on the human pharyngeal\ncell and mediates bacterial adherence to host cells","volume":"350","author":"Jin","year":"2005","journal-title":"J. Mol. Biol."},{"issue":"2","key":"2026090216340148700_cit35","doi-asserted-by":"publisher","first-page":"324","DOI":"10.1111\/j.1574-6968.2006.00140.x","article-title":"Adhesion characteristics of Listeria\nadhesion protein (LAP)-expressing Escherichia coli to Caco-2 cells\nand of recombinant LAP to eukaryotic receptor Hsp60 as examined in\na surface plasmon resonance sensor","volume":"256","author":"Kim","year":"2006","journal-title":"FEMS Microbiol.\nLett."},{"issue":"1\u20133","key":"2026090216340148700_cit36","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/S0014-5793(02)02580-2","article-title":"Enhanced activation of bound plasminogen\non Staphylococcus aureus by staphylokinase","volume":"517","author":"Molkanen","year":"2002","journal-title":"FEBS Lett."},{"issue":"2","key":"2026090216340148700_cit37","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1128\/IAI.72.2.931-936.2004","article-title":"Heat shock\nprotein 60 acts as a receptor for the Listeria adhesion protein in\nCaco-2 cells","volume":"72","author":"Wampler","year":"2004","journal-title":"Infect. Immun."},{"issue":"1","key":"2026090216340148700_cit38","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.mib.2011.10.012","article-title":"Recurrent infections and immune evasion\nstrategies of Staphylococcus aureus","volume":"15","author":"Kim","year":"2012","journal-title":"Curr. Opin.\nMicrobiol."},{"issue":"1","key":"2026090216340148700_cit39","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1002\/mbo3.55","article-title":"Extracellular\nproteases are key mediators of Staphylococcus aureus virulence via\nthe global modulation of virulence-determinant stability","volume":"2","author":"Kolar","year":"2013","journal-title":"MicrobiologyOpen"},{"issue":"19","key":"2026090216340148700_cit40","doi-asserted-by":"publisher","first-page":"5279","DOI":"10.1128\/JB.00369-11","article-title":"Proteolytic\ncleavage inactivates the Staphylococcus aureus lipoteichoic acid synthase","volume":"193","author":"Wormann","year":"2011","journal-title":"J. Bacteriol."},{"issue":"10","key":"2026090216340148700_cit41","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003361","article-title":"Factors contributing to the biofilm-deficient\nphenotype of Staphylococcus aureus sarA mutants","volume":"3","author":"Tsang","year":"2008","journal-title":"PLoS One"},{"issue":"3","key":"2026090216340148700_cit42","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1002\/bimj.200810425","article-title":"Simultaneous inference\nin general parametric models","volume":"50","author":"Hothorn","year":"2008","journal-title":"Biom. J."},{"issue":"7","key":"2026090216340148700_cit43","doi-asserted-by":"publisher","first-page":"4206","DOI":"10.1128\/IAI.71.7.4206-4211.2003","article-title":"Mutation of sarA in Staphylococcus\naureus limits biofilm formation","volume":"71","author":"Beenken","year":"2003","journal-title":"Infect. Immun."},{"issue":"5","key":"2026090216340148700_cit44","doi-asserted-by":"publisher","first-page":"1634","DOI":"10.1128\/IAI.06134-11","article-title":"Impact of extracellular\nnuclease production on the biofilm phenotype of Staphylococcus aureus\nunder in vitro and in vivo conditions","volume":"80","author":"Beenken","year":"2012","journal-title":"Infect.\nImmun."}],"container-title":["ACS Infectious Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsinfecdis.9b00291","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/6\/2\/237\/67004474\/acsinfecdis.9b00291.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article-pdf\/6\/2\/237\/67004474\/acsinfecdis.9b00291.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:34:09Z","timestamp":1788381249000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aidcbc\/article\/6\/2\/237\/618200\/Exploiting-Correlations-between-Protein-Abundance"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,13]]},"references-count":44,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,11,13]]},"published-print":{"date-parts":[[2020,2,14]]}},"URL":"https:\/\/doi.org\/10.1021\/acsinfecdis.9b00291","relation":{},"ISSN":["2373-8227"],"issn-type":[{"value":"2373-8227","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,13]]}}}