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Bacteria circumvent these problems with the aid of iron storage proteins where Fe3+ is deposited and, when necessary, mobilized as Fe2+ for metabolic requirements. In Pseudomonas aeruginosa, Fe3+ is compartmentalized in bacterioferritin (BfrB), and its mobilization as Fe2+ requires specific binding of a ferredoxin (Bfd) to reduce the stored Fe3+. Blocking the BfrB-Bfd complex leads to irreversible iron accumulation in BfrB and cytosolic iron deprivation. Consequently, given the intracellular iron sufficiency requirement for biofilm development, we hypothesized that blocking the BfrB-Bfd interaction in P. aeruginosa would impair biofilm development. Our results show that planktonic and biofilm-embedded cells where the BfrB-Bfd complex is blocked exhibit cytosolic iron deficiency, and poorly developed biofilms, even in iron-sufficient culture conditions. These results underscore inhibition of the BfrB-Bfd complex as a rational target to dysregulate iron homeostasis and possibly control biofilms.<\/jats:p>","DOI":"10.1021\/acsinfecdis.9b00398","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T08:47:35Z","timestamp":1578041255000},"page":"447-458","source":"Crossref","is-referenced-by-count":49,"title":["Inhibiting Iron Mobilization from Bacterioferritin\nin\n                    <i>Pseudomonas aeruginosa<\/i>\n                    Impairs Biofilm Formation\nIrrespective of Environmental Iron Availability"],"prefix":"10.1021","volume":"6","author":[{"given":"Anabel","family":"Soldano","sequence":"first","affiliation":[{"name":"Louisiana State University , , 232 Choppin Hall , , ,","place":["Baton Rouge, Louisiana, United States, 70803"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huili","family":"Yao","sequence":"additional","affiliation":[{"name":"Louisiana State University , , 232 Choppin Hall , , ,","place":["Baton Rouge, Louisiana, United States, 70803"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8944-9813","authenticated-orcid":true,"given":"Josephine R.","family":"Chandler","sequence":"additional","affiliation":[{"name":"University of Kansas , , 1200 Sunnyside Avenue , , ,","place":["Lawrence, Kansas, United States, 66045"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5692-5497","authenticated-orcid":true,"given":"Mario","family":"Rivera","sequence":"additional","affiliation":[{"name":"Louisiana State University , , 232 Choppin Hall , , ,","place":["Baton Rouge, Louisiana, United States, 70803"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,1,3]]},"reference":[{"key":"2026090216340350300_cit1","doi-asserted-by":"publisher","first-page":"6558","DOI":"10.1128\/JB.173.20.6558-6567.1991","article-title":"Optical sectioning of microbial biofilms","volume":"173","author":"Lawrence","year":"1991","journal-title":"J. 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