{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:23:55Z","timestamp":1791246235260,"version":"4.1.0"},"reference-count":80,"publisher":"American Chemical Society (ACS)","issue":"4","license":[{"start":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T00:00:00Z","timestamp":1583280000000},"content-version":"vor","delay-in-days":13,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_ccby_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["5R01AI090141"],"award-info":[{"award-number":["5R01AI090141"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI103058"],"award-info":[{"award-number":["R01AI103058"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000061","name":"Fogarty International Center","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100000061","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1107194"],"award-info":[{"award-number":["OPP1107194"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Most phenotypic screens aiming to discover new antimalarial chemotypes begin with low cost, high-throughput tests against the asexual blood stage (ABS) of the malaria parasite life cycle. Compounds active against the ABS are then sequentially tested in more difficult assays that predict whether a compound has other beneficial attributes. Although applying this strategy to new chemical libraries may yield new leads, repeated iterations may lead to diminishing returns and the rediscovery of chemotypes hitting well-known targets. Here, we adopted a different strategy to find starting points, testing \u223c70,000 open source small molecules from the Global Health Chemical Diversity Library for activity against the liver stage, mature sexual stage, and asexual blood stage malaria parasites in parallel. In addition, instead of using an asexual assay that measures accumulated parasite DNA in the presence of compound (SYBR green), a real time luciferase-dependent parasite viability assay was used that distinguishes slow-acting (delayed death) from fast-acting compounds. Among 382 scaffolds with the activity confirmed by dose response (&amp;lt;10 \u03bcM), we discovered 68 novel delayed-death, 84 liver stage, and 68 stage V gametocyte inhibitors as well. Although 89% of the evaluated compounds had activity in only a single life cycle stage, we discovered six potent (half-maximal inhibitory concentration of &amp;lt;1 \u03bcM) multistage scaffolds, including a novel cytochrome bc1 chemotype. Our data further show the luciferase-based assays have higher sensitivity. Chemoinformatic analysis of positive and negative compounds identified scaffold families with a strong enrichment for activity against specific or multiple stages.<\/jats:p>","DOI":"10.1021\/acsinfecdis.9b00482","type":"journal-article","created":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T17:20:40Z","timestamp":1582219240000},"page":"613-628","source":"Crossref","is-referenced-by-count":43,"title":["Probing the Open\nGlobal Health Chemical Diversity\nLibrary for Multistage-Active Starting Points for Next-Generation\nAntimalarials"],"prefix":"10.1021","volume":"6","author":[{"given":"Matthew","family":"Abraham","sequence":"first","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Gagaring","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute , , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa L.","family":"Martino","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manu","family":"Vanaerschot","sequence":"additional","affiliation":[{"name":"Columbia University Irving Medical Center , , , ,","place":["New York, New York, United States, 10032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Plouffe","sequence":"additional","affiliation":[{"name":"Genomics Institute of the Novartis Research Foundation , 10675 John Jay Hopkins Drive , , ,","place":["San Diego, California, United States, 92121"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaeson","family":"Calla","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla P.","family":"Godinez-Macias","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan Y.","family":"Du","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Wree","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yevgeniya","family":"Antonova-Koch","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korina","family":"Eribez","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeline R.","family":"Luth","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabine","family":"Ottilie","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Fidock","sequence":"additional","affiliation":[{"name":"Columbia University Irving Medical Center , , , ,","place":["New York, New York, United States, 10032"]},{"name":"Columbia University Irving Medical Center , , , ,","place":["New York, New York, United States, 10032"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5754-3407","authenticated-orcid":true,"given":"Case W.","family":"McNamara","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute , , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4049-2113","authenticated-orcid":true,"given":"Elizabeth A.","family":"Winzeler","sequence":"additional","affiliation":[{"name":"University of California San Diego , , 9500 Gilman Drive , , ,","place":["La Jolla, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,2,20]]},"reference":[{"key":"2026090313170429100_cit1","doi-asserted-by":"crossref","DOI":"10.30875\/f309483f-en","volume-title":"World malaria report 2018","author":"World Health Organization.","year":"2018"},{"issue":"12","key":"2026090313170429100_cit2","doi-asserted-by":"publisher","first-page":"1223","DOI":"10.1016\/S1473-3099(17)30475-9","article-title":"Treating malaria:\nnew drugs for a new era","volume":"17","author":"Shanks","year":"2017","journal-title":"Lancet Infect. Dis."},{"issue":"2","key":"2026090313170429100_cit3","doi-asserted-by":"publisher","first-page":"e000211","DOI":"10.1136\/bmjgh-2016-000211","article-title":"Developing an expanded\nvector control toolbox for malaria elimination","volume":"2","author":"Killeen","year":"2017","journal-title":"BMJ. Glob Health"},{"issue":"Suppl 1","key":"2026090313170429100_cit4","doi-asserted-by":"publisher","first-page":"S11","DOI":"10.1186\/1475-2875-7-S1-S11","article-title":"Malaria eradication:\nthe economic,\nfinancial and institutional challenge","volume":"7","author":"Mills","year":"2008","journal-title":"Malar.\nJ."},{"issue":"7","key":"2026090313170429100_cit5","doi-asserted-by":"publisher","first-page":"a025619","DOI":"10.1101\/cshperspect.a025619","article-title":"Antimalarial Drug Resistance: A Threat to Malaria Elimination","volume":"7","author":"Menard","year":"2017","journal-title":"Cold Spring Harbor Perspect. Med."},{"issue":"2","key":"2026090313170429100_cit6","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/0035-9203(90)90258-G","article-title":"An estimation of the\nnumber of malaria sporozoites ejected by a feeding mosquito","volume":"84","author":"Rosenberg","year":"1990","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"issue":"7","key":"2026090313170429100_cit7","doi-asserted-by":"publisher","first-page":"4363","DOI":"10.1128\/IAI.73.7.4363-4369.2005","article-title":"Quantitative dynamics\nof Plasmodium yoelii sporozoite\ntransmission by infected anopheline mosquitoes","volume":"73","author":"Medica","year":"2005","journal-title":"Infect. Immun."},{"issue":"10","key":"2026090313170429100_cit8","doi-asserted-by":"publisher","first-page":"1925","DOI":"10.1111\/j.1462-5822.2008.01176.x","article-title":"Malarial\nproteases and host cell egress: an \u2019emerging\u2019 cascade","volume":"10","author":"Blackman","year":"2008","journal-title":"Cell. Microbiol."},{"issue":"2","key":"2026090313170429100_cit9","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1038\/nm1350","article-title":"Quantitative imaging of Plasmodium\ntransmission from mosquito to mammal","volume":"12","author":"Amino","year":"2006","journal-title":"Nat. Med."},{"issue":"5","key":"2026090313170429100_cit10","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/S0035-9203(01)90016-1","article-title":"Genesis, sequestration\nand survival of Plasmodium falciparum gametocytes:\nparameter estimates from fitting a model to malariatherapy data","volume":"95","author":"Eichner","year":"2001","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"2026090313170429100_cit11","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1186\/1475-2875-3-24","article-title":"Gametocytogenesis:\nthe puberty of Plasmodium falciparum","volume":"3","author":"Talman","year":"2004","journal-title":"Malar.\nJ."},{"key":"2026090313170429100_cit12","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/978-1-62703-026-7_2","article-title":"Production\nof Plasmodium falciparum gametocytes in\nvitro","volume":"923","author":"Saliba","year":"2012","journal-title":"Methods Mol. Biol."},{"issue":"3","key":"2026090313170429100_cit13","doi-asserted-by":"publisher","first-page":"702","DOI":"10.4269\/ajtmh.16-0779","article-title":"Hemolytic\nPotential of Tafenoquine in Female Volunteers Heterozygous for Glucose-6-Phosphate\nDehydrogenase (G6PD) Deficiency (G6PD Mahidol Variant) versus G6PD-Normal\nVolunteers","volume":"97","author":"Rueangweerayut","year":"2017","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"10","key":"2026090313170429100_cit14","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1016\/j.drudis.2015.06.009","article-title":"Supporting\nmalaria elimination with 21st century antimalarial agent drug discovery","volume":"20","author":"Diagana","year":"2015","journal-title":"Drug Discovery Today"},{"key":"2026090313170429100_cit15","doi-asserted-by":"publisher","first-page":"590","DOI":"10.1039\/C8MD90009D","article-title":"Correction: Recent\nupdates in the discovery and development\nof novel antimalarial drug candidates","volume":"9","author":"Okombo","year":"2018","journal-title":"MedChemComm"},{"key":"2026090313170429100_cit16","doi-asserted-by":"publisher","first-page":"754","DOI":"10.3389\/fphar.2017.00754","article-title":"Development in Assay\nMethods for in Vitro Antimalarial Drug Efficacy Testing: A Systematic\nReview","volume":"8","author":"Sinha","year":"2017","journal-title":"Front. Pharmacol."},{"issue":"6061","key":"2026090313170429100_cit17","doi-asserted-by":"publisher","first-page":"1372","DOI":"10.1126\/science.1211936","article-title":"Imaging of Plasmodium\nliver stages to drive next-generation antimalarial drug discovery","volume":"334","author":"Meister","year":"2011","journal-title":"Science"},{"issue":"8","key":"2026090313170429100_cit18","doi-asserted-by":"publisher","first-page":"e0135139","DOI":"10.1371\/journal.pone.0135139","article-title":"A New Set\nof Chemical Starting Points with Plasmodium falciparum Transmission-Blocking\nPotential for Antimalarial Drug Discovery","volume":"10","author":"Almela","year":"2015","journal-title":"PLoS\nOne"},{"issue":"4","key":"2026090313170429100_cit19","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1021\/acsinfecdis.5b00143","article-title":"High-Throughput Luciferase-Based Assay for the Discovery\nof Therapeutics That Prevent Malaria","volume":"2","author":"Swann","year":"2016","journal-title":"ACS Infect.\nDis."},{"issue":"9","key":"2026090313170429100_cit20","doi-asserted-by":"publisher","first-page":"5060","DOI":"10.1128\/AAC.02727-13","article-title":"KAF156 is an antimalarial\nclinical candidate with potential for use in prophylaxis, treatment,\nand prevention of disease transmission","volume":"58","author":"Kuhen","year":"2014","journal-title":"Antimicrob.\nAgents Chemother."},{"issue":"5996","key":"2026090313170429100_cit21","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1126\/science.1193225","article-title":"Spiroindolones,\na potent compound class for the treatment of malaria","volume":"329","author":"Rottmann","year":"2010","journal-title":"Science"},{"issue":"15","key":"2026090313170429100_cit22","doi-asserted-by":"publisher","first-page":"5540","DOI":"10.1021\/jm200592f","article-title":"Structure-guided lead optimization\nof triazolopyrimidine-ring substituents identifies potent Plasmodium\nfalciparum dihydroorotate dehydrogenase inhibitors with clinical candidate\npotential","volume":"54","author":"Coteron","year":"2011","journal-title":"J. Med. Chem."},{"issue":"387","key":"2026090313170429100_cit23","doi-asserted-by":"publisher","first-page":"eaad9735","DOI":"10.1126\/scitranslmed.aad9735","article-title":"Antimalarial efficacy of MMV390048,\nan inhibitor of Plasmodium phosphatidylinositol 4-kinase","volume":"9","author":"Paquet","year":"2017","journal-title":"Sci. Transl. Med."},{"issue":"26","key":"2026090313170429100_cit24","doi-asserted-by":"publisher","first-page":"9059","DOI":"10.1073\/pnas.0802982105","article-title":"In silico\nactivity\nprofiling reveals the mechanism of action of antimalarials discovered\nin a high-throughput screen","volume":"105","author":"Plouffe","year":"2008","journal-title":"Proc. Natl. Acad.\nSci. U. S. A."},{"issue":"7296","key":"2026090313170429100_cit25","doi-asserted-by":"publisher","first-page":"311","DOI":"10.1038\/nature09099","article-title":"Chemical genetics of Plasmodium falciparum","volume":"465","author":"Guiguemde","year":"2010","journal-title":"Nature"},{"issue":"5","key":"2026090313170429100_cit26","doi-asserted-by":"publisher","first-page":"1803","DOI":"10.1128\/AAC.48.5.1803-1806.2004","article-title":"Simple and\ninexpensive fluorescence-based technique for high-throughput antimalarial\ndrug screening","volume":"48","author":"Smilkstein","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"issue":"7296","key":"2026090313170429100_cit27","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1038\/nature09107","article-title":"Thousands of chemical starting points for antimalarial\nlead identification","volume":"465","author":"Gamo","year":"2010","journal-title":"Nature"},{"key":"2026090313170429100_cit28","unstructured":"University of Dundee.\n          \n          2018) Gates Library screen for HepG2 cell viability.  ChEMBL, https:\/\/www.ebi.ac.uk\/chembl\/document_report_card\/CHEMBL3507680\/."},{"issue":"1\u20133","key":"2026090313170429100_cit29","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S0169-409X(00)00129-0","article-title":"Experimental and\ncomputational approaches to estimate solubility and permeability in\ndrug discovery and development settings","volume":"46","author":"Lipinski","year":"2001","journal-title":"Adv.\nDrug Delivery Rev."},{"issue":"1","key":"2026090313170429100_cit30","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1021\/ci600343x","article-title":"ADME evaluation in\ndrug discovery. 7. Prediction of oral absorption by correlation and\nclassification","volume":"47","author":"Hou","year":"2007","journal-title":"J. Chem. Inf. Model."},{"issue":"21","key":"2026090313170429100_cit31","doi-asserted-by":"publisher","first-page":"6752","DOI":"10.1021\/jm901241e","article-title":"Escape from\nflatland: increasing\nsaturation as an approach to improving clinical success","volume":"52","author":"Lovering","year":"2009","journal-title":"J. Med. Chem."},{"issue":"2","key":"2026090313170429100_cit32","doi-asserted-by":"publisher","first-page":"e0005373","DOI":"10.1371\/journal.pntd.0005373","article-title":"A high-throughput\nphenotypic screen identifies clofazimine as a potential treatment\nfor cryptosporidiosis","volume":"11","author":"Love","year":"2017","journal-title":"PLoS Neglected Trop.\nDis."},{"issue":"1","key":"2026090313170429100_cit33","doi-asserted-by":"publisher","first-page":"3805","DOI":"10.1038\/s41467-018-05777-2","article-title":"A high throughput\nscreen for next-generation leads targeting malaria parasite transmission","volume":"9","author":"Delves","year":"2018","journal-title":"Nat. Commun."},{"issue":"6419","key":"2026090313170429100_cit34","doi-asserted-by":"publisher","first-page":"eaat9446","DOI":"10.1126\/science.aat9446","article-title":"Open-source discovery\nof chemical leads for next-generation chemoprotective antimalarials","volume":"362","author":"Antonova-Koch","year":"2018","journal-title":"Science"},{"issue":"2","key":"2026090313170429100_cit35","doi-asserted-by":"publisher","first-page":"e1001169","DOI":"10.1371\/journal.pmed.1001169","article-title":"The activities of current\nantimalarial drugs on the life cycle stages of Plasmodium: a comparative\nstudy with human and rodent parasites","volume":"9","author":"Delves","year":"2012","journal-title":"PLoS\nMed."},{"issue":"1","key":"2026090313170429100_cit36","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.chom.2015.12.001","article-title":"High-throughput assay and discovery of small molecules\nthat interrupt malaria transmission","volume":"19","author":"Plouffe","year":"2016","journal-title":"Cell Host\nMicrobe"},{"issue":"10","key":"2026090313170429100_cit37","doi-asserted-by":"publisher","first-page":"3583","DOI":"10.1096\/fj.11-187401","article-title":"Identifying apicoplast-targeting\nantimalarials using high-throughput compatible approaches","volume":"25","author":"Ekland","year":"2011","journal-title":"FASEB J."},{"issue":"10","key":"2026090313170429100_cit38","doi-asserted-by":"publisher","first-page":"3485","DOI":"10.1128\/AAC.00527-07","article-title":"Multiple\nantibiotics exert delayed effects against\nthe Plasmodium falciparum apicoplast","volume":"51","author":"Dahl","year":"2007","journal-title":"Antimicrob.\nAgents Chemother."},{"issue":"7","key":"2026090313170429100_cit39","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1016\/j.ijpara.2006.12.010","article-title":"Delayed parasite elimination\nin human\ninfections treated with clindamycin parallels \u2019delayed death\u2019\nof Plasmodium falciparum in vitro","volume":"37","author":"Burkhardt","year":"2007","journal-title":"Int. J. Parasitol."},{"issue":"1","key":"2026090313170429100_cit40","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1128\/AAC.00808-06","article-title":"Inhibitors\nof nonhousekeeping functions of the apicoplast defy delayed death\nin Plasmodium falciparum","volume":"51","author":"Ramya","year":"2007","journal-title":"Antimicrob. Agents\nChemother."},{"issue":"1","key":"2026090313170429100_cit41","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/j.molbiopara.2005.09.007","article-title":"High efficiency transfection of Plasmodium\nberghei facilitates novel selection procedures","volume":"145","author":"Janse","year":"2006","journal-title":"Mol. Biochem. Parasitol."},{"issue":"2","key":"2026090313170429100_cit42","doi-asserted-by":"publisher","first-page":"e1000010","DOI":"10.1371\/journal.ppat.1000010","article-title":"Hepatocyte\npermissiveness to Plasmodium infection is\nconveyed by a short and structurally conserved region of the CD81\nlarge extracellular domain","volume":"4","author":"Yalaoui","year":"2008","journal-title":"PLoS Pathog."},{"issue":"32","key":"2026090313170429100_cit43","doi-asserted-by":"publisher","first-page":"11468","DOI":"10.1073\/pnas.0503386102","article-title":"Murine malaria parasite\nsequestration: CD36 is the major receptor, but cerebral pathology\nis unlinked to sequestration","volume":"102","author":"Franke-Fayard","year":"2005","journal-title":"Proc. Natl. Acad.\nSci. U. S. A."},{"issue":"6372","key":"2026090313170429100_cit44","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1126\/science.aan4472","article-title":"Mapping\nthe malaria parasite druggable genome by using in vitro evolution\nand chemogenomics","volume":"359","author":"Cowell","year":"2018","journal-title":"Science"},{"issue":"3","key":"2026090313170429100_cit45","doi-asserted-by":"publisher","first-page":"226","DOI":"10.2174\/187152610791163336","article-title":"Plasmodium dihydroorotate\ndehydrogenase: a promising\ntarget for novel anti-malarial chemotherapy","volume":"10","author":"Phillips","year":"2010","journal-title":"Infect. Disord.: Drug Targets"},{"issue":"3","key":"2026090313170429100_cit46","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1016\/j.cbpa.2010.03.020","article-title":"Apparent activity\nin high-throughput\nscreening: origins of compound-dependent assay interference","volume":"14","author":"Thorne","year":"2010","journal-title":"Curr. Opin. Chem. Biol."},{"issue":"9","key":"2026090313170429100_cit47","doi-asserted-by":"publisher","first-page":"3585","DOI":"10.1073\/pnas.0813345106","article-title":"Mechanism of PTC124\nactivity in cell-based luciferase assays of nonsense codon suppression","volume":"106","author":"Auld","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"3","key":"2026090313170429100_cit48","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1016\/j.parint.2014.09.011","article-title":"Direct evidence\nfor the atovaquone action on the Plasmodium cytochrome bc1 complex","volume":"64","author":"Siregar","year":"2015","journal-title":"Parasitol. Int."},{"issue":"6","key":"2026090313170429100_cit49","doi-asserted-by":"publisher","first-page":"1195","DOI":"10.4269\/ajtmh.14-0553","article-title":"Inhibition of cytochrome\nbc1 as a strategy for single-dose, multi-stage antimalarial therapy","volume":"92","author":"Stickles","year":"2015","journal-title":"Am. J. Trop. Med. Hyg."},{"issue":"24","key":"2026090313170429100_cit50","doi-asserted-by":"publisher","first-page":"6285","DOI":"10.1073\/pnas.1804492115","article-title":"Selection\nof Plasmodium falciparum cytochrome B mutants\nby putative PfNDH2 inhibitors","volume":"115","author":"Lane","year":"2018","journal-title":"Proc. Natl. Acad.\nSci. U. S. A."},{"issue":"1","key":"2026090313170429100_cit51","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.chom.2015.12.001","article-title":"High-Throughput\nAssay and Discovery of Small Molecules that Interrupt Malaria Transmission","volume":"19","author":"Plouffe","year":"2016","journal-title":"Cell Host Microbe"},{"issue":"5","key":"2026090313170429100_cit52","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1093\/jac\/dkv493","article-title":"A chemical susceptibility profile of the Plasmodium falciparum transmission\nstages by complementary cell-based gametocyte assays","volume":"71","author":"D\u2019Alessandro","year":"2016","journal-title":"J. Antimicrob. 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