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Here, we construct a single-atom nanozyme using graphitic carbon nitride nanosheets modified by copper (Cu-g-C3N4). This Cu-g-C3N4 nanosheet possesses both glucose oxidase-like and peroxidase-like activities responsible for reactive-oxygen-species generation by a cascade reaction to eradicate Gram-positive and Gram-negative multidrug-resistant bacteria. Cu-g-C3N4 is introduced into polycaprolactone (PCL) by electrospinning to obtain (Cu-g-C3N4\/PCL) nanofibers, which can be used as a dressing for bacterially infected wounds. It is demonstrated that Cu-g-C3N4\/PCL nanofiber dressings can eradicate bacterial infections and accelerate wound healing in a mouse model with a skin wound.<\/jats:p>","DOI":"10.1021\/acsnano.4c12348","type":"journal-article","created":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T22:55:16Z","timestamp":1741992916000},"page":"10816-10828","source":"Crossref","is-referenced-by-count":94,"title":["Single-Atom Cu Anchored\non Carbon Nitride as a Bifunctional\nGlucose Oxidase and Peroxidase Nanozyme for Antibacterial Therapy"],"prefix":"10.1021","volume":"19","author":[{"given":"Fan","family":"Wu","sequence":"first","affiliation":[{"name":"the First Affiliated Hospital of Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang, China, 325035"]},{"name":"University of Groningen and University Medical Center Groningen , , Antonius Deusinglaan 1 , ,","place":["Groningen, The Netherlands, 9713 AV"]},{"name":"Nankai University , , ,","place":["Tianjin, China, 300071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaran","family":"Wang","sequence":"additional","affiliation":[{"name":"the First Affiliated Hospital of Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang, China, 325035"]},{"name":"University of Groningen and University Medical Center Groningen , , Antonius Deusinglaan 1 , ,","place":["Groningen, The Netherlands, 9713 AV"]},{"name":"Nankai University , , ,","place":["Tianjin, China, 300071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanfeng","family":"Li","sequence":"additional","affiliation":[{"name":"the First Affiliated Hospital of Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang, China, 325035"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9534-795X","authenticated-orcid":true,"given":"Linqi","family":"Shi","sequence":"additional","affiliation":[{"name":"Nankai University , , ,","place":["Tianjin, China, 300071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Yuan","sequence":"additional","affiliation":[{"name":"University of Groningen and University Medical Center Groningen , , Antonius Deusinglaan 1 , ,","place":["Groningen, The Netherlands, 9713 AV"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijin","family":"Ren","sequence":"additional","affiliation":[{"name":"University of Groningen and University Medical Center Groningen , , Hanzeplein 1 , ,","place":["Groningen, The Netherlands, 9700 RB"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0760-8900","authenticated-orcid":true,"given":"Henny C.","family":"van der Mei","sequence":"additional","affiliation":[{"name":"University of Groningen and University Medical Center Groningen , , Antonius Deusinglaan 1 , ,","place":["Groningen, The Netherlands, 9713 AV"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1738-7857","authenticated-orcid":true,"given":"Yong","family":"Liu","sequence":"additional","affiliation":[{"name":"the First Affiliated Hospital of Wenzhou Medical University , , , ,","place":["Wenzhou, Zhejiang, China, 325035"]},{"name":"Nankai University , , ,","place":["Tianjin, China, 300071"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"2026081813161437900_cit1","doi-asserted-by":"publisher","first-page":"698","DOI":"10.1038\/s41586-021-03899-0","article-title":"Rational Design of a New Antibiotic\nClass for Drug-Resistant Infections","volume":"597","author":"Durand-Reville","year":"2021","journal-title":"Nature"},{"key":"2026081813161437900_cit2","doi-asserted-by":"publisher","first-page":"3731","DOI":"10.1038\/s41467-022-31500-3","article-title":"TPGS-Based and S-Thanatin\nFunctionalized Nanorods for Overcoming\nDrug Resistance in Klebsiella pneumonia","volume":"13","author":"Wang","year":"2022","journal-title":"Nat. Commun."},{"key":"2026081813161437900_cit3","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1002\/mabi.201300561","article-title":"Electrospun\nNanofibers as Dressings\nfor Chronic Wound Care: Advances, Challenges, and Future Prospects","volume":"14","author":"Abrigo","year":"2014","journal-title":"Macromol. Biosci."},{"key":"2026081813161437900_cit4","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.jhin.2013.06.015","article-title":"Modification\nof the Surfaces of Medical Devices to Prevent Microbial Adhesion and\nBiofilm Formation","volume":"85","author":"Desrousseaux","year":"2013","journal-title":"J. Hosp. 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Ed."},{"key":"2026081813161437900_cit26","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201900093","article-title":"Designing Defective\nCrystalline Carbon Nitride to Enable Selective\nCO2 Photoreduction in the Gas Phase","volume":"29","author":"Xia","year":"2019","journal-title":"Adv. Funct. Mater."},{"key":"2026081813161437900_cit27","doi-asserted-by":"publisher","first-page":"8481","DOI":"10.1002\/anie.201702386","article-title":"Highly Ordered\nNitrogen-Rich Mesoporous Carbon Nitrides and their Superior Performance\nfor Sensing and Photocatalytic Hydrogen Generation","volume":"56","author":"Mane","year":"2017","journal-title":"Angew. Chemie Int. Ed."},{"key":"2026081813161437900_cit28","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202103978","article-title":"Near-Field\nDrives Long-Lived Shallow Trapping of Polymeric C3N4 for Efficient Photocatalytic Hydrogen Evolution","volume":"31","author":"Wang","year":"2021","journal-title":"Adv. Funct. 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Mater."},{"key":"2026081813161437900_cit54","first-page":"39","article-title":"The Use of Modified Masson\u2019s\nTrichrome Staining\nin Collagen Evaluation in Wound Healing Study","volume":"3","author":"Suvika","year":"2012","journal-title":"Malays. J. Vet. 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