{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:09:17Z","timestamp":1789052957137,"version":"build-2803163510"},"reference-count":76,"publisher":"American Chemical Society (ACS)","issue":"31","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000183","name":"Army Research Office","doi-asserted-by":"publisher","award":["W911NF-19-2-0026"],"award-info":[{"award-number":["W911NF-19-2-0026"]}],"id":[{"id":"10.13039\/100000183","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014989","name":"Chan Zuckerberg Initiative","doi-asserted-by":"publisher","award":["2023-332386"],"award-info":[{"award-number":["2023-332386"]}],"id":[{"id":"10.13039\/100014989","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Charles Trimble Fund at the California Institute of Technology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Recent advances have demonstrated the application of microcavity-containing hydrogel microparticles, known as nanovials, for massively parallel and high-throughput screening of therapeutic T cell populations for adoptive cell therapies. Nanovial cavities coated with peptide-MHC (pMHC) or antigen tetramers selectively bind to their cognate T cell receptor (TCR) or chimeric antigen receptor (CAR) to activate T cells and capture secreted cytokines. However, binding of tetramers or recombinantly expressed antigen by T cells may not reflect physiological T cell activation or cytotoxicity, as the binding interface is not fully representative of the natural immunological synapse formed between T cells and professional antigen-presenting cells (APCs). Here, we leverage the recent discovery of an ESCRT- and ALIX-binding region (EABR) sequence to generate antigen-presenting vesicles and cancer-mimicking exosomes from standard HEK293T and Expi293F cell cultures. EABR-mediated vesicles present natural, full-length oncologically relevant membrane proteins embedded in lipid bilayers to functionalize the nanovial cavity with cell-like membranes. Hydrogel nanovials functionalized with the EABR-mediated vesicles show improved T cell capture of 1G4 T cells and enhanced induction of secretion in HER2 CAR-T cells compared to hydrogel surfaces functionalized with recombinantly expressed soluble proteins.<\/jats:p>","DOI":"10.1021\/acsnano.5c19139","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:16:49Z","timestamp":1785849409000},"page":"21569-21585","source":"Crossref","is-referenced-by-count":1,"title":["Functionalizing\nHydrogel Nanovials with Vesicles Mimicking\nAntigen-Presenting Vesicles and Cancer Exosomes Improves T Cell Capture\nand Activation"],"prefix":"10.1021","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1526-1399","authenticated-orcid":true,"given":"Blade A.","family":"Olson","sequence":"first","affiliation":[{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]},{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Mellody","sequence":"additional","affiliation":[{"name":"University of California, Los Angeles , , , ,","place":["Los Angeles, California, United States, 90095"]},{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0267-1097","authenticated-orcid":true,"given":"Citradewi","family":"Soemardy","sequence":"additional","affiliation":[{"name":"University of California, Los Angeles , , , ,","place":["Los Angeles, California, United States, 90095"]},{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Mao","sequence":"additional","affiliation":[{"name":"University of California , , , ,","place":["Los Angeles, California, United States, 90095"]},{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7091-4471","authenticated-orcid":true,"given":"Anna","family":"Mei","sequence":"additional","affiliation":[{"name":"ETH Zu\u0308rich , , ,","place":["Basel, Switzerland, 4056"]},{"name":"University of Zurich , , ,","place":["Zu\u0308rich, Switzerland, 8057"]},{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Lippert","sequence":"additional","affiliation":[{"name":"University of California San Francisco , , , ,","place":["San Francisco, California, United States, 94143"]},{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4923-9568","authenticated-orcid":true,"given":"Magnus A. G.","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"University of California San Francisco , , , ,","place":["San Francisco, California, United States, 94143"]},{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3942-4284","authenticated-orcid":true,"given":"Dino","family":"Di Carlo","sequence":"additional","affiliation":[{"name":"University of California , , , ,","place":["Los Angeles, California, United States, 90095"]},{"name":"University of California , , , ,","place":["Los Angeles, California, United States, 90095"]},{"name":"Partillion Bioscience , , ,","place":["Pasadena, California, United States, 91107"]},{"name":"University of California , , , ,","place":["Los Angeles, California, United States, 90095"]},{"name":"California NanoSystems Institute , , ,","place":["Los Angeles, California, United States, 90095"]},{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9785-5018","authenticated-orcid":true,"given":"Stephen L.","family":"Mayo","sequence":"additional","affiliation":[{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]},{"name":"California Institute of Technology , , , ,","place":["Pasadena, California, United States, 91125"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"issue":"16","key":"2026081111124253400_cit1","doi-asserted-by":"publisher","first-page":"1507","DOI":"10.1056\/NEJMoa1407222","article-title":"Chimeric Antigen Receptor T Cells for Sustained\nRemissions in Leukemia","volume":"371","author":"Maude","year":"2014","journal-title":"N. Engl. J. Med."},{"issue":"26","key":"2026081111124253400_cit2","doi-asserted-by":"publisher","first-page":"2545","DOI":"10.1056\/NEJMoa1708566","article-title":"Chimeric Antigen Receptor\nT Cells in Refractory B-Cell Lymphomas","volume":"377","author":"Schuster","year":"2017","journal-title":"N. Engl.\nJ. Med."},{"issue":"5","key":"2026081111124253400_cit3","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1038\/s44222-023-00148-z","article-title":"Biomaterials to Enhance Adoptive Cell Therapy","volume":"2","author":"Eckman","year":"2024","journal-title":"Nat. Rev. Bioeng."},{"issue":"9967","key":"2026081111124253400_cit4","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/S0140-6736(14)61403-3","article-title":"T Cells Expressing CD19 Chimeric Antigen Receptors\nfor Acute Lymphoblastic Leukaemia in Children and Young Adults: A\nPhase 1 Dose-Escalation Trial","volume":"385","author":"Lee","year":"2015","journal-title":"Lancet"},{"issue":"8","key":"2026081111124253400_cit5","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1056\/NEJMoa1103849","article-title":"Chimeric\nAntigen Receptor\u2013Modified T Cells in Chronic Lymphoid Leukemia","volume":"365","author":"Porter","year":"2011","journal-title":"N. Engl. J. Med."},{"issue":"280","key":"2026081111124253400_cit6","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa3643","article-title":"Adoptive\nCellular Therapy: A Race\nto the Finish Line","volume":"7","author":"June","year":"2015","journal-title":"Sci. Transl. Med."},{"issue":"36","key":"2026081111124253400_cit7","doi-asserted-by":"publisher","first-page":"13139","DOI":"10.1073\/pnas.1409155111","article-title":"Diversity\nand Clonal Selection in the Human T-Cell Repertoire","volume":"111","author":"Qi","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"9","key":"2026081111124253400_cit8","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.tibtech.2014.06.007","article-title":"Towards Efficient\nCancer Immunotherapy: Advances in Developing Artificial Antigen-Presenting\nCells","volume":"32","author":"Eggermont","year":"2014","journal-title":"Trends Biotechnol."},{"issue":"4","key":"2026081111124253400_cit9","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1097\/PPO.0b013e3181eb33a6","article-title":"Artificial Antigen-Presenting\nCells for Use in Adoptive\nImmunotherapy","volume":"16","author":"Turtle","year":"2010","journal-title":"Cancer J."},{"issue":"8","key":"2026081111124253400_cit10","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1038\/nri3020","article-title":"Interrogating\nthe Repertoire: Broadening\nthe Scope of Peptide-MHC Multimer Analysis","volume":"11","author":"Davis","year":"2011","journal-title":"Nat. Rev. Immunol."},{"issue":"5425","key":"2026081111124253400_cit11","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1126\/science.285.5425.221","article-title":"The Immunological Synapse: A Molecular\nMachine Controlling T Cell Activation","volume":"285","author":"Grakoui","year":"1999","journal-title":"Science"},{"issue":"14","key":"2026081111124253400_cit12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2320442121","article-title":"Defining T Cell Receptor Repertoires Using\nNanovial-Based Binding and Functional Screening","volume":"121","author":"Koo","year":"2024","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"5","key":"2026081111124253400_cit13","doi-asserted-by":"publisher","first-page":"7242","DOI":"10.1021\/acsnano.1c11420","article-title":"Suspendable Hydrogel Nanovials for Massively Parallel\nSingle-Cell Functional Analysis and Sorting","volume":"16","author":"de Rutte","year":"2022","journal-title":"ACS Nano"},{"key":"2026081111124253400_cit14","doi-asserted-by":"crossref","unstructured":"Soemardy, C.; Mei, A.; Castellanos-Rueda, R.; Espinoza, N. G.; Kizerwetter, M.; Spangler, J. B.; Reddy, S. T.; Di Carlo, D.\n          Screening of a Pooled Library\nof Chimeric Antigen Receptor T Cells Based on Secretory Function\n          bioRxiv\n          2025\n          2025.2007.2002.662376\n          10.1101\/2025.07.02.662376.","DOI":"10.1101\/2025.07.02.662376"},{"issue":"12","key":"2026081111124253400_cit15","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202203163","article-title":"Cell-Sized Lipid Vesicles as Artificial Antigen-Presenting\nCells for Antigen-Specific T Cell Activation","volume":"12","author":"Chen","year":"2023","journal-title":"Adv. Healthcare Mater."},{"key":"2026081111124253400_cit16","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.972790","article-title":"Recent Advances in Surface Modification of Micro- and Nano-Scale\nBiomaterials with Biological Membranes and Biomolecules","volume":"10","author":"Abesekara","year":"2022","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"2","key":"2026081111124253400_cit17","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1038\/nbt.4047","article-title":"Scaffolds That Mimic\nAntigen-Presenting Cells Enable Ex Vivo Expansion of Primary T Cells","volume":"36","author":"Cheung","year":"2018","journal-title":"Nat. Biotechnol."},{"issue":"1","key":"2026081111124253400_cit18","doi-asserted-by":"publisher","DOI":"10.3390\/cells13010033","article-title":"Determination\nof Binding Affinity\nof Antibodies to HIV-1 Recombinant Envelope Glycoproteins, Pseudoviruses,\nInfectious Molecular Clones, and Cell-Expressed Trimeric gp160 Using\nMicroscale Thermophoresis","volume":"13","author":"Basu","year":"2024","journal-title":"Cells"},{"issue":"6","key":"2026081111124253400_cit19","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1038\/nsmb.3195","article-title":"Nanodiscs for Structural and Functional Studies of\nMembrane Proteins","volume":"23","author":"Denisov","year":"2016","journal-title":"Nat. Struct. Mol. Biol."},{"issue":"49","key":"2026081111124253400_cit20","doi-asserted-by":"publisher","first-page":"46183","DOI":"10.1021\/acsami.9b17015","article-title":"Self-Assembled Human Adipose-Derived\nStem Cell-Derived Extracellular Vesicle-Functionalized Biotin-Doped\nPolypyrrole Titanium with Long-Term Stability and Potential Osteoinductive\nAbility","volume":"11","author":"Chen","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026081111124253400_cit21","doi-asserted-by":"crossref","unstructured":"Antich-Rossello\u0301, M.; Forteza-Genestra, M. A.; Calvo, J.; Gaya\u0300, A.; Monjo, M.; Ramis, J. M.\n          Platelet-Derived\nExtracellular Vesicle Functionalization of Ti Implants\n          J. Visualized Exp.\n          2021, No. 174, 10.3791\/62781.","DOI":"10.3791\/62781"},{"issue":"6","key":"2026081111124253400_cit22","doi-asserted-by":"publisher","DOI":"10.3390\/nano11061445","article-title":"Extracellular-Vesicle-Based\nCoatings\nEnhance Bioactivity of Titanium Implants-SurfEV","volume":"11","author":"Pansani","year":"2021","journal-title":"Nanomaterials"},{"issue":"3","key":"2026081111124253400_cit23","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1016\/j.matt.2023.01.012","article-title":"Exosome\nMembrane-Coated Nanosystems: Exploring Biomedical Applications in\nCancer Diagnosis and Therapy","volume":"6","author":"Shao","year":"2023","journal-title":"Matter"},{"issue":"6","key":"2026081111124253400_cit24","doi-asserted-by":"publisher","first-page":"1690","DOI":"10.1021\/acs.bioconjchem.9b00240","article-title":"PLGA Microspheres\nCoated with Cancer Cell-Derived Vesicles for Improved Internalization\ninto Antigen-Presenting Cells and Immune Stimulation","volume":"30","author":"Jung","year":"2019","journal-title":"Bioconjugate Chem."},{"issue":"5","key":"2026081111124253400_cit25","doi-asserted-by":"publisher","first-page":"2666","DOI":"10.1021\/acsabm.0c00181","article-title":"Improving\nTumor Targeting of Exosomal Membrane-Coated Polymeric Nanoparticles\nby Conjugation with Aptamers","volume":"3","author":"Han","year":"2020","journal-title":"ACS Appl. Bio\nMater."},{"issue":"45","key":"2026081111124253400_cit26","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202102643","article-title":"Antigen-Specific Stimulation\nand Expansion of CAR-T Cells Using Membrane Vesicles as Target Cell\nSurrogates","volume":"17","author":"Ukrainskaya","year":"2021","journal-title":"Small"},{"key":"2026081111124253400_cit27","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1016\/j.ejps.2016.10.009","article-title":"Cell Type-Specific\nand Common Characteristics\nof Exosomes Derived from Mouse Cell Lines: Yield, Physicochemical\nProperties, and Pharmacokinetics","volume":"96","author":"Charoenviriyakul","year":"2017","journal-title":"Eur. J. Pharm.\nSci."},{"key":"2026081111124253400_cit28","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.jconrel.2016.02.037","article-title":"Exosomes as Therapeutics: The Implications of Molecular\nComposition and Exosomal Heterogeneity","volume":"228","author":"Ferguson","year":"2016","journal-title":"J. Controlled\nRelease"},{"issue":"7","key":"2026081111124253400_cit29","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1038\/nnano.2017.56","article-title":"Peptide-MHC-Based Nanomedicines\nfor Autoimmunity Function as T-Cell Receptor Microclustering Devices","volume":"12","author":"Singha","year":"2017","journal-title":"Nat. Nanotechnol."},{"issue":"1","key":"2026081111124253400_cit30","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.bcmd.2005.04.004","article-title":"Direct Stimulation\nof Nai\u0308ve T Cells By Antigen-Presenting Cell Vesicles","volume":"35","author":"Sprent","year":"2005","journal-title":"Blood Cells, Mol., Dis."},{"issue":"11","key":"2026081111124253400_cit31","doi-asserted-by":"publisher","first-page":"2380","DOI":"10.1016\/j.cell.2023.04.024","article-title":"ESCRT Recruitment\nto SARS-Cov-2 Spike Induces Virus-Like Particles That Improve mRNA\nVaccines","volume":"186","author":"Hoffmann","year":"2023","journal-title":"Cell"},{"issue":"51","key":"2026081111124253400_cit32","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2501046122","article-title":"Recruiting ESCRT to Single-Chain\nHeterotrimer Peptide MHCI Releases Antigen-Presenting Vesicles That\nStimulate T Cells Selectively","volume":"122","author":"Olson","year":"2025","journal-title":"Proc. Natl. Acad.\nSci. U. S. A."},{"issue":"112","key":"2026081111124253400_cit33","doi-asserted-by":"publisher","DOI":"10.3791\/54041","article-title":"Expression and Purification of Virus-like\nParticles for Vaccination","author":"Arevalo","year":"2016","journal-title":"J. Visualized Exp."},{"issue":"3","key":"2026081111124253400_cit34","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1111\/imm.13317","article-title":"Epithelial Cell-Derived\nCD83 Restores Immune Tolerance\nin the Airway Mucosa by Inducing Regulatory T-Cell Differentiation","volume":"163","author":"Mo","year":"2021","journal-title":"Immunology"},{"issue":"1","key":"2026081111124253400_cit35","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-48200-9","article-title":"PD-1\/CD80(+) Small Extracellular Vesicles from Immunocytes\nInduce Cold Tumours Featured with Enhanced Adaptive Immunosuppression","volume":"15","author":"Zhang","year":"2024","journal-title":"Nat. Commun."},{"issue":"1","key":"2026081111124253400_cit36","doi-asserted-by":"publisher","DOI":"10.1038\/s41698-022-00287-3","article-title":"Small Extracellular Vesicle PD-L1\nIn Cancer: The Knowns and Unknowns","volume":"6","author":"Yu","year":"2022","journal-title":"NPJ. Precis.\nOncol."},{"issue":"7718","key":"2026081111124253400_cit37","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1038\/s41586-018-0392-8","article-title":"Exosomal\nPD-L1 Contributes to Immunosuppression and as Associated\nwith Anti-PD-1 Response","volume":"560","author":"Chen","year":"2018","journal-title":"Nature"},{"issue":"Pt 13","key":"2026081111124253400_cit38","doi-asserted-by":"publisher","first-page":"2885","DOI":"10.1242\/jcs.145334","article-title":"Peptide-Independent\nStabilization of MHC Class I Molecules Breaches Cellular Quality Control","volume":"127","author":"Hein","year":"2014","journal-title":"J. Cell Sci."},{"issue":"1","key":"2026081111124253400_cit39","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-023-04899-8","article-title":"Large Libraries of Single-Chain Trimer Peptide-MHCs\nEnable Antigen-Specific CD8+ T Cell Discovery and Analysis","volume":"6","author":"Chour","year":"2023","journal-title":"Commun. Biol."},{"key":"2026081111124253400_cit40","volume-title":"Molecular Technologies\nfor Antigen-Based Immunity. Ph.D","author":"Chour","year":"2021"},{"key":"2026081111124253400_cit41","doi-asserted-by":"crossref","unstructured":"Lees, R.; Tempest, R.; Law, A.; Aubert, D.; Davies, O. G.; Williams, S.; Peake, N.; Peacock, B.\n          Single Extracellular Vesicle Transmembrane\nProtein Characterization by Nano-Flow Cytometry\n          J. Visualized Exp.\n          2022, No. 185, 10.3791\/64020.","DOI":"10.3791\/64020"},{"issue":"3","key":"2026081111124253400_cit42","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1038\/s12276-025-01422-3","article-title":"Generation\nof Hypoimmunogenic Universal iPS Cells Through HLA-Type Gene Knockout","volume":"57","author":"Kim","year":"2025","journal-title":"Exp. Mol. Med."},{"issue":"3","key":"2026081111124253400_cit43","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1084\/jem.183.3.1161","article-title":"B Lymphocytes\nSecrete\nAntigen-Presenting Vesicles","volume":"183","author":"Raposo","year":"1996","journal-title":"J. Exp. Med."},{"issue":"4","key":"2026081111124253400_cit44","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1038\/s41577-022-00763-8","article-title":"The Roles\nof Extracellular Vesicles in the Immune System","volume":"23","author":"Buzas","year":"2023","journal-title":"Nat. Rev. Immunol."},{"issue":"9","key":"2026081111124253400_cit45","doi-asserted-by":"publisher","first-page":"6116","DOI":"10.4049\/jimmunol.180.9.6116","article-title":"Single and Dual Amino\nAcid Substitutions in\nTCR CDRs Can Enhance Antigen-Specific T Cell Functions","volume":"180","author":"Robbins","year":"2008","journal-title":"J. Immunol."},{"issue":"45","key":"2026081111124253400_cit46","doi-asserted-by":"publisher","first-page":"E10702","DOI":"10.1073\/pnas.1810653115","article-title":"Isolation and Characterization\nof NY-ESO-1-Specific T Cell Receptors Restricted on Various MHC Molecules","volume":"115","author":"Bethune","year":"2018","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"10","key":"2026081111124253400_cit47","doi-asserted-by":"publisher","first-page":"2919","DOI":"10.1002\/eji.200425297","article-title":"Dissecting Cytotoxic\nT Cell Responses Towards\nthe NY-ESO-1 Protein by Peptide\/MHC-Specific Antibody Fragments","volume":"34","author":"Held","year":"2004","journal-title":"Eur. J. Immunol."},{"issue":"14","key":"2026081111124253400_cit48","doi-asserted-by":"publisher","first-page":"5784","DOI":"10.1073\/pnas.0901425106","article-title":"Rational\nDevelopment\nof High-Affinity T-Cell Receptor-Like Antibodies","volume":"106","author":"Stewart-Jones","year":"2009","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"1","key":"2026081111124253400_cit49","doi-asserted-by":"publisher","first-page":"97","DOI":"10.4049\/jimmunol.154.1.97","article-title":"CD80 (B7) and CD86\n(B70) Provide Similar Costimulatory Signals for T Cell Proliferation,\nCytokine Production, and Generation of CTL","volume":"154","author":"Lanier","year":"1995","journal-title":"J. Immunol."},{"issue":"25","key":"2026081111124253400_cit50","doi-asserted-by":"publisher","first-page":"22783","DOI":"10.1021\/acsnano.4c18391","article-title":"Specific\nStimulation and Multiplex Secretion Analysis of T Cells\nUsing a Suspendable Hydrogel Platform Enables Immunophenotyping at\nthe Single-Cell Scale","volume":"19","author":"Kizerwetter","year":"2025","journal-title":"ACS Nano"},{"key":"2026081111124253400_cit51","doi-asserted-by":"crossref","unstructured":"Baghdasarian, S.; Wang, Q.; Langerman, J.; Mao, Z.; Wright, H.; Gee, C.; Cheng, D.; McLaughlin, J.; Lee, J. K.; Chen, X.; et al.\n          Systematic\nMapping of Emergent Transcriptional\nStates in Interacting Single-Cell Dyads By Cell-Cell-Seq\n          bioRxiv\n          2026\n          2026.02.05.704136\n          10.64898\/2026.02.05.704136.","DOI":"10.64898\/2026.02.05.704136"},{"issue":"5","key":"2026081111124253400_cit52","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202451666","article-title":"Linking Epitope-Specific T-Cell Receptors\nto IFN\u03b3 Secretion Using Nanovial Technology","volume":"55","author":"van den Dijssel","year":"2025","journal-title":"Eur. J. Immunol."},{"issue":"5","key":"2026081111124253400_cit53","doi-asserted-by":"publisher","first-page":"1871","DOI":"10.1158\/0008-5472.CAN-10-1872","article-title":"Trastuzumab Has Preferential\nActivity Against Breast Cancers Driven by HER2 Homodimers","volume":"71","author":"Ghosh","year":"2011","journal-title":"Cancer Res."},{"issue":"2","key":"2026081111124253400_cit54","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adq8114","article-title":"Molecular\nDynamics at\nImmune Synapse Lipid Rafts Influence the Cytolytic Behavior of CAR\nT Cells","volume":"11","author":"Gad","year":"2025","journal-title":"Sci. Adv."},{"issue":"3","key":"2026081111124253400_cit55","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.devcel.2022.01.002","article-title":"ICAM-1-Mediated Adhesion is a Prerequisite\nfor Exosome-Induced T\nCell Suppression","volume":"57","author":"Zhang","year":"2022","journal-title":"Dev.l Cell"},{"issue":"8","key":"2026081111124253400_cit56","doi-asserted-by":"publisher","first-page":"2099","DOI":"10.1038\/s41591-023-02452-y","article-title":"Mesothelin-Targeting\nT Cell Receptor Fusion Construct Cell Therapy in Refractory Solid\nTumors: Phase 1\/2 Trial Interim Results","volume":"29","author":"Hassan","year":"2023","journal-title":"Nat.\nMed."},{"issue":"1","key":"2026081111124253400_cit57","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2024-010581","article-title":"XCL1-Secreting CEA\nCAR-T Cells Enhance Endogenous CD8(+)\nT Cell Responses to Tumor Neoantigens to Confer a Long-Term Antitumor\nImmunity","volume":"13","author":"Li","year":"2025","journal-title":"J. ImmunoTher. Cancer"},{"issue":"4","key":"2026081111124253400_cit58","doi-asserted-by":"publisher","first-page":"724","DOI":"10.1038\/s41591-022-01726-1","article-title":"PSMA-Targeting TGF\u03b2-Insensitive Armored\nCAR T Cells in Metastatic Castration-Resistant Prostate Cancer: A\nPhase 1 Trial","volume":"28","author":"Narayan","year":"2022","journal-title":"Nat. Med."},{"issue":"1","key":"2026081111124253400_cit59","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-024-02858-x","article-title":"Multiparametric\nProfiling of HER2-Enriched\nExtracellular Vesicles in Breast Cancer Using Single Extracellular\nVEsicle Nanoscopy","volume":"22","author":"Jiang","year":"2024","journal-title":"J. Nanobiotechnol."},{"issue":"11","key":"2026081111124253400_cit60","doi-asserted-by":"publisher","first-page":"7045","DOI":"10.1021\/acs.nanolett.7b03734","article-title":"Biologically\nInspired Design of Nanoparticle Artificial Antigen-Presenting Cells\nfor Immunomodulation","volume":"17","author":"Hickey","year":"2017","journal-title":"Nano Lett."},{"issue":"6","key":"2026081111124253400_cit61","doi-asserted-by":"publisher","first-page":"1565","DOI":"10.1039\/D4LC01056F","article-title":"Multi-Reactive Hydrogel\nNanovials for Temporal Control of Secretion Capture From Antibody-Secreting\nCells","volume":"25","author":"Mellody","year":"2025","journal-title":"Lab Chip"},{"issue":"1","key":"2026081111124253400_cit62","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-39367-8","article-title":"SEC-Seq:\nAssociation of Molecular\nSignatures with Antibody Secretion in Thousands of Single Human Plasma\nCells","volume":"14","author":"Cheng","year":"2023","journal-title":"Nat. Commun."},{"key":"2026081111124253400_cit63","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-024-01112-w","article-title":"Linking Single-Cell\nTranscriptomes with Secretion Using SEC-Seq","volume":"20","author":"Langerman","year":"2025","journal-title":"Nat. Protoc."},{"issue":"3","key":"2026081111124253400_cit64","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1038\/s41565-023-01560-7","article-title":"Associating Growth\nFactor Secretions\nand Transcriptomes of Single Cells in Nanovials Using SEC-Seq","volume":"19","author":"Udani","year":"2024","journal-title":"Nat. Nanotechnol."},{"issue":"1","key":"2026081111124253400_cit65","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-49123-1","article-title":"Optimizing\nCell Therapy by Sorting Cells with\nHigh Extracellular Vesicle Secretion","volume":"15","author":"Koo","year":"2024","journal-title":"Nat. Commun."},{"issue":"6478","key":"2026081111124253400_cit66","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau6977","article-title":"The Biology, Function,\nand Biomedical Applications\nof Exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"issue":"7812","key":"2026081111124253400_cit67","doi-asserted-by":"publisher","first-page":"S14","DOI":"10.1038\/d41586-020-01769-9","article-title":"How Extracellular\nVesicles Can Enhance Drug Delivery","volume":"582","author":"Keener","year":"2020","journal-title":"Nature"},{"issue":"7812","key":"2026081111124253400_cit68","doi-asserted-by":"publisher","first-page":"S16","DOI":"10.1038\/d41586-020-01770-2","article-title":"Inside the\nStem-Cell Pharmaceutical Factory","volume":"582","author":"Eisenstein","year":"2020","journal-title":"Nature"},{"issue":"492","key":"2026081111124253400_cit69","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aav8521","article-title":"Advances in Therapeutic Applications of Extracellular\nVesicles","volume":"11","author":"Wiklander","year":"2019","journal-title":"Sci. Transl. Med."},{"key":"2026081111124253400_cit70","doi-asserted-by":"publisher","first-page":"49","DOI":"10.2147\/IJN.S390720","article-title":"Extracellular Vesicles Expressing CD19 Antigen Improve Expansion\nand Efficacy of CD19-Targeted CAR-T Cells","volume":"Volume 18","author":"Zhang","year":"2023","journal-title":"Int.\nJ. Nanomed."},{"issue":"3","key":"2026081111124253400_cit71","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adp9009","article-title":"Engineered Extracellular Vesicles with DR5 Agonistic\nscFvs Simultaneously Target Tumor and Immunosuppressive Stromal Cells","volume":"11","author":"Guo","year":"2025","journal-title":"Sci. Adv."},{"issue":"38","key":"2026081111124253400_cit72","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202301019","article-title":"Engineered Cytokine-Primed Extracellular Vesicles with High PD-L1\nExpression Ameliorate Type 1 Diabetes","volume":"19","author":"Wang","year":"2023","journal-title":"Small"},{"issue":"18","key":"2026081111124253400_cit73","doi-asserted-by":"publisher","first-page":"8392","DOI":"10.1073\/pnas.90.18.8392","article-title":"Production\nof high-titer helper-free retroviruses by transient transfection","volume":"90","author":"Pear","year":"1993","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026081111124253400_cit74","unstructured":"Renner, C.; Wadle, A.; Cerundolo, V.; Stewart-Jones, G.; Jones, Y. E.\n          High Affinity T-Cell Receptor-Like\nNY-ESO-1 Peptide Antibodies, Methods, and Uses Thereof. WIPO (PCT)\nPCT\/IB2010\/000597. 2010."},{"issue":"7","key":"2026081111124253400_cit75","doi-asserted-by":"publisher","first-page":"3145","DOI":"10.4049\/jimmunol.168.7.3145","article-title":"Cutting\nEdge: Single-Chain Trimers of MHC Class I Molecules Form Stable Structures\nThat Potently Stimulate Antigen-Specific T Cells And B Cells","volume":"168","author":"Yu","year":"2002","journal-title":"J. Immunol."},{"issue":"3","key":"2026081111124253400_cit76","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.1038\/nprot.2006.121","article-title":"Generation of Peptide-MHC class I\nComplexes Through UV-Mediated Ligand Exchange","volume":"1","author":"Rodenko","year":"2006","journal-title":"Nat. Protoc."}],"container-title":["ACS Nano"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsnano.5c19139","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/20\/31\/21569\/66328596\/acsnano.5c19139.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/20\/31\/21569\/66328596\/acsnano.5c19139.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T15:43:24Z","timestamp":1786463004000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancac3\/article\/20\/31\/21569\/5236616\/Functionalizing-Hydrogel-Nanovials-with-Vesicles"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,29]]},"references-count":76,"journal-issue":{"issue":"31","published-online":{"date-parts":[[2026,7,29]]},"published-print":{"date-parts":[[2026,8,11]]}},"URL":"https:\/\/doi.org\/10.1021\/acsnano.5c19139","relation":{},"ISSN":["1936-0851","1936-086X"],"issn-type":[{"value":"1936-0851","type":"print"},{"value":"1936-086X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,29]]}}}