{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:55:19Z","timestamp":1787072119314,"version":"build-2736575974"},"reference-count":37,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000181","name":"Air Force Office of Scientific Research","doi-asserted-by":"publisher","award":["FA9550-23-1-0090"],"award-info":[{"award-number":["FA9550-23-1-0090"]}],"id":[{"id":"10.13039\/100000181","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000078","name":"Division of Materials Research","doi-asserted-by":"publisher","award":["DMR-2011924"],"award-info":[{"award-number":["DMR-2011924"]}],"id":[{"id":"10.13039\/100000078","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Potassium ion (K+) dynamics are central to cardiac electrophysiology, with early disruptions in K+ flux often preceding arrhythmia and contractile dysfunction. However, current sensing technologies, such as patch-clamp, microelectrode arrays (MEAs), and fluorescent indicators, either lack chemical specificity for K+ or are unsuitable for long-term, single-cell analysis. Conventional ion-selective electrodes (ISEs), while more selective, are limited by bulk-phase design and poor spatial resolution. To address these limitations, we present KINESIS (K+-Ion Nano-Electrode Selective Interface System), a nanofabricated platform that enables direct, label-free potentiometric measurement of K+ gradients with single-cell precision. KINESIS features high-aspect-ratio nanopillars coated with a valinomycin-based K+ recognition membrane, forming a stable, noninvasive interface with human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). This architecture allows localized, Nernstian sensing of K+ efflux or depletion without disrupting cell membranes. Pharmacological validation shows distinct potential shifts in response to caffeine and ouabain. KINESIS thus offers a highly selective, spatially resolved approach for studying K+ handling in cardiotoxicity screening and patient-specific disease modeling.<\/jats:p>","DOI":"10.1021\/acsnano.5c21944","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:35:56Z","timestamp":1785854156000},"page":"22413-22421","source":"Crossref","is-referenced-by-count":0,"title":["Potassium-Selective\nNanoelectrode Arrays for Single-Cell\nProfiling of Human iPSC-Derived Cardiomyocytes"],"prefix":"10.1021","volume":"20","author":[{"given":"Dhivya Pushpa","family":"Meganathan","sequence":"first","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romeo","family":"Banzon","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Casanova","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7841-5244","authenticated-orcid":true,"given":"Einollah","family":"Sarikhani","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1927-4795","authenticated-orcid":true,"given":"Kuldeep","family":"Mahato","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hillary","family":"Vu","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Reade","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iswerya","family":"Ambika Devarajan","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anum","family":"Tahir","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]},{"name":"Brigham Young University , , , ,","place":["Provo, Utah, United States, 84602"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lekshmi","family":"Sasi","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leah","family":"Sadr","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4921-9674","authenticated-orcid":true,"given":"Joseph","family":"Wang","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0139-3219","authenticated-orcid":true,"given":"Zeinab","family":"Jahed","sequence":"additional","affiliation":[{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]},{"name":"University of California San Diego , , La Jolla, , ,","place":["San Diego, California, United States, 92093"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"2026081810274494100_cit1","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1038\/s41467-024-55571-6","article-title":"Intelligent\nIn-Cell Electrophysiology: Reconstructing Intracellular Action Potentials\nUsing a Physics-Informed Deep Learning Model Trained on Nanoelectrode\nArray Recordings","volume":"16","author":"Rahmani","year":"2025","journal-title":"Nat. Commun"},{"key":"2026081810274494100_cit2","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1038\/nnano.2012.8","article-title":"Intracellular Recording\nof Action Potentials by Nanopillar Electroporation","volume":"7","author":"Xie","year":"2012","journal-title":"Nat. Nanotechnol"},{"key":"2026081810274494100_cit3","doi-asserted-by":"publisher","first-page":"2253","DOI":"10.1038\/s41467-022-29726-2","article-title":"Nanocrown Electrodes\nfor Parallel and Robust Intracellular Recording of Cardiomyocytes","volume":"13","author":"Jahed","year":"2022","journal-title":"Nat. Commun"},{"key":"2026081810274494100_cit4","doi-asserted-by":"publisher","first-page":"2500158","DOI":"10.1002\/admi.202500158","article-title":"A Dynamic Spike Sorter for Multiscale\nNanoelectrode Array Recordings","volume":"12","author":"Shukla","year":"2025","journal-title":"Adv. Mater.\nInterfaces"},{"key":"2026081810274494100_cit5","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1038\/s41378-024-00817-y","article-title":"Supra- and Sub-Threshold Intracellular-like\nRecording of 2D and 3D Neuronal Networks Using Nanopillar Electrode\nArrays","volume":"10","author":"Shukla","year":"2024","journal-title":"Microsyst. Nanoeng"},{"key":"2026081810274494100_cit6","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.coelec.2019.04.026","article-title":"Recent Advances in Sensing and Biosensing\nwith Arrays of Nanoelectrodes","volume":"16","author":"Karimian","year":"2019","journal-title":"Curr. Opin. 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Solid State\nElectrochem"}],"container-title":["ACS Nano"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsnano.5c21944","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/20\/32\/22413\/66378017\/acsnano.5c21944.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/20\/32\/22413\/66378017\/acsnano.5c21944.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:22:05Z","timestamp":1787070125000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancac3\/article\/20\/32\/22413\/5242121\/Potassium-Selective-Nanoelectrode-Arrays-for"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,3]]},"references-count":37,"journal-issue":{"issue":"32","published-online":{"date-parts":[[2026,8,3]]},"published-print":{"date-parts":[[2026,8,18]]}},"URL":"https:\/\/doi.org\/10.1021\/acsnano.5c21944","relation":{},"ISSN":["1936-0851","1936-086X"],"issn-type":[{"value":"1936-0851","type":"print"},{"value":"1936-086X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,3]]}}}