{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:10:15Z","timestamp":1791544215944,"version":"4.3.4"},"reference-count":54,"publisher":"American Chemical Society (ACS)","issue":"34","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35 GM130332"],"award-info":[{"award-number":["R35 GM130332"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RM1 GM131981"],"award-info":[{"award-number":["RM1 GM131981"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U24GM139166"],"award-info":[{"award-number":["U24GM139166"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["24CSA1257409"],"award-info":[{"award-number":["24CSA1257409"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008428","name":"Department of Energy and Climate Change","doi-asserted-by":"publisher","award":["DE-AC02-76SF00515"],"award-info":[{"award-number":["DE-AC02-76SF00515"]}],"id":[{"id":"10.13039\/100008428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Hypertrophic cardiomyopathy (HCM) is the most common monogenic inherited heart disease and is a major cause of sudden death in individuals under 35 years of age. HCM is associated with progressive tissue-level disarray and subcellular disorganization in individual cardiomyocytes. Mutations in \u03b2-cardiac myosin (MYH7), the second most common genetic cause of HCM, commonly result in changes in sarcomeric force production, but how this leads to altered cell- and tissue-level organization is unclear. Here, we use cryo-electron tomography (cryo-ET) to bridge the molecular and cellular scales by visualizing the nanoscale organization of individual myosin-containing thick filaments within sarcomeres of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Compared to isogenic wild-type controls, hiPSC-CMs expressing P710R MYH7 exhibit pronounced disruption of the hexagonal packing of thick filaments within individual sarcomeres, which would be difficult to visualize using conventional light microscopy or even room-temperature electron microscopy. We also observe ribosome infiltration into areas of sarcomeric disorder for both wild-type and P710R MYH7 hiPSC-CMs, suggesting that disordered regions may be sites of local proteostasis or remodeling. Together, these data illuminate how altered myosin activity can propagate to yield dramatic changes in sarcomeric organization in HCM.<\/jats:p>","DOI":"10.1021\/acsnano.6c03369","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:08:22Z","timestamp":1787065702000},"page":"23748-23755","source":"Crossref","is-referenced-by-count":1,"title":["Cryo-Electron Tomography\nReveals Nanoscale Thick Filament\nDisorganization in MYH7 P710R Hypertrophic Cardiomyopathy Cardiomyocytes"],"prefix":"10.1021","volume":"20","author":[{"given":"Magda","family":"Zaoralova\u0301","sequence":"first","affiliation":[{"name":"Stanford University , , , ,","place":["Menlo Park, California, United States, 94025"]},{"name":"Stanford University , , , ,","place":["Stanford, California, United States, 94305"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Yoniles","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine , , , ,","place":["Stanford, California, United States, 94305"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prerna","family":"Giri","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine , , , ,","place":["Stanford, California, United States, 94305"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard G.","family":"Held","sequence":"additional","affiliation":[{"name":"Stanford University , , , ,","place":["Stanford, California, United States, 94305"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison","family":"Vander Roest","sequence":"additional","affiliation":[{"name":"University of Michigan , , , ,","place":["Ann Arbor, Michigan, United States, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4452-2663","authenticated-orcid":true,"given":"Peter D.","family":"Dahlberg","sequence":"additional","affiliation":[{"name":"Stanford University , , , ,","place":["Menlo Park, California, United States, 94025"]},{"name":"Stanford University , , , ,","place":["Stanford, California, United States, 94305"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Bernstein","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine , , , ,","place":["Stanford, California, United States, 94305"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6096-4600","authenticated-orcid":true,"given":"Alexander R.","family":"Dunn","sequence":"additional","affiliation":[{"name":"Stanford University , , , ,","place":["Stanford, California, United States, 94305"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5329-4300","authenticated-orcid":true,"given":"Leeya","family":"Engel","sequence":"additional","affiliation":[{"name":"Technion - Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200003"]},{"name":"Technion - Israel Institute of Technology , , ,","place":["Haifa, Israel, 3200003"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"issue":"8","key":"2026090306572924500_cit1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000001052","article-title":"Heart Disease and Stroke Statistics\u25002022\nUpdate: A Report From the American Heart Association","volume":"145","author":"Tsao","year":"2022","journal-title":"Circulation"},{"issue":"2","key":"2026090306572924500_cit2","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1038\/nrcardio.2011.159","article-title":"Mechanisms of disease:\nHypertrophic\ncardiomyopathy","volume":"9","author":"Frey","year":"2012","journal-title":"Nat. Rev. Cardiol."},{"issue":"14","key":"2026090306572924500_cit3","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1161\/CIRCULATIONAHA.117.033200","article-title":"Genotype\nand lifetime burden of disease in hypertrophic\ncardiomyopathy: insights from the sarcomeric human cardiomyopathy\nregistry (share)","volume":"138","author":"Ho","year":"2018","journal-title":"Circulation"},{"issue":"7","key":"2026090306572924500_cit4","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1161\/CIRCRESAHA.117.311059","article-title":"Hypertrophic\ncardiomyopathy: genetics, pathogenesis,\nclinical manifestations, diagnosis, and therapy","volume":"121","author":"Marian","year":"2017","journal-title":"Circ. Res."},{"issue":"4","key":"2026090306572924500_cit5","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1016\/S0092-8674(01)00242-2","article-title":"The genetic basis for cardiomyopathy:\nfrom mutation\nidentification to mechanistic paradigms","volume":"104","author":"Seidman","year":"2001","journal-title":"Cell"},{"issue":"5","key":"2026090306572924500_cit6","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1007\/s00424-019-02259-2","article-title":"Three perspectives\non the molecular basis of hypercontractility caused by hypertrophic\ncardiomyopathy mutations","volume":"471","author":"Spudich","year":"2019","journal-title":"Pflugers Arch."},{"key":"2026090306572924500_cit7","doi-asserted-by":"publisher","first-page":"975076","DOI":"10.3389\/fphys.2022.975076","article-title":"Hypertrophic cardiomyopathy: Mutations\nto mechanisms to therapies","volume":"13","author":"Kawana","year":"2022","journal-title":"Front. Physiol."},{"issue":"3","key":"2026090306572924500_cit8","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1083\/jcb.201101100","article-title":"Cellular mechanisms\nof cardiomyopathy","volume":"194","author":"Harvey","year":"2011","journal-title":"J. Cell Biol."},{"issue":"9","key":"2026090306572924500_cit9","doi-asserted-by":"publisher","first-page":"1746","DOI":"10.1016\/j.biocel.2005.04.011","article-title":"Cardiomyocytes structure, function and associated pathologies","volume":"37","author":"Woodcock","year":"2005","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"2026090306572924500_cit10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-0-387-84847-1_1","article-title":"The sarcomere and sarcomerogenesis","volume":"642","author":"Ehler","year":"2008","journal-title":"Adv. Exp. Med. Biol."},{"key":"2026090306572924500_cit11","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.42144","article-title":"Muscle-specific stress fibers give rise to\nsarcomeres in cardiomyocytes","volume":"7","author":"Fenix","year":"2018","journal-title":"eLife"},{"issue":"1","key":"2026090306572924500_cit12","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.devcel.2017.12.012","article-title":"Force generation via \u03b2-cardiac myosin, titin, and \u03b1-actinin drives cardiac sarcomere assembly from cell-matrix adhesions","volume":"44","author":"Chopra","year":"2018","journal-title":"Dev. Cell"},{"issue":"7","key":"2026090306572924500_cit13","doi-asserted-by":"publisher","first-page":"985","DOI":"10.1093\/europace\/euaa348","article-title":"Arrhythmogenic\npotential of myocardial disarray in\nhypertrophic cardiomyopathy: genetic basis, functional consequences\nand relation to sudden cardiac death","volume":"23","author":"Finocchiaro","year":"2021","journal-title":"EP Europace"},{"issue":"5","key":"2026090306572924500_cit14","doi-asserted-by":"publisher","first-page":"962","DOI":"10.1111\/joa.13058","article-title":"Myoarchitectural\ndisarray of hypertrophic cardiomyopathy begins pre-birth","volume":"235","author":"Garcia-Canadilla","year":"2019","journal-title":"J. Anat."},{"key":"2026090306572924500_cit15","volume-title":"Cardiomyopathies","author":"Bajusz","year":"1973"},{"issue":"24","key":"2026090306572924500_cit16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2025030118","article-title":"Hypertrophic cardiomyopathy \u03b2-cardiac myosin mutation (P710R) leads to hypercontractility\nby disrupting super relaxed state","volume":"118","author":"Vander Roest","year":"2021","journal-title":"Proc. Natl.\nAcad. Sci. U. S. A."},{"issue":"5","key":"2026090306572924500_cit17","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1161\/CIRCGENETICS.108.821314","article-title":"Prevalence of sarcomere\nprotein gene mutations in preadolescent children with hypertrophic\ncardiomyopathy","volume":"2","author":"Kaski","year":"2009","journal-title":"Circ. Cardiovasc. Genet."},{"issue":"1","key":"2026090306572924500_cit18","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1073\/pnas.0909468107","article-title":"Myosin ATP turnover\nrate is a mechanism involved in thermogenesis in resting skeletal\nmuscle fibers","volume":"107","author":"Stewart","year":"2010","journal-title":"Proc. Natl. Acad. Sci. U. S.\nA."},{"key":"2026090306572924500_cit19","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1601959","article-title":"Biophysical\nproperties of human \u03b2-cardiac myosin with converter-domain\nmutations that cause hypertrophic cardiomyopathy","volume":"3","author":"Kawana","year":"2017","journal-title":"Sci. Adv."},{"issue":"21","key":"2026090306572924500_cit20","doi-asserted-by":"publisher","first-page":"1714","DOI":"10.1161\/CIRCULATIONAHA.121.053575","article-title":"Altered cardiac energetics and mitochondrial dysfunction\nin hypertrophic cardiomyopathy","volume":"144","author":"Ranjbarvaziri","year":"2021","journal-title":"Circulation"},{"issue":"5596","key":"2026090306572924500_cit21","doi-asserted-by":"publisher","first-page":"1209","DOI":"10.1126\/science.1076184","article-title":"Macromolecular\narchitecture in eukaryotic cells visualized\nby cryoelectron tomography","volume":"298","author":"Medalia","year":"2002","journal-title":"Science"},{"issue":"2","key":"2026090306572924500_cit22","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1016\/j.jmb.2015.09.030","article-title":"In situ cryo-electron tomography:\na post-reductionist approach to structural biology","volume":"428","author":"Asano","year":"2016","journal-title":"J. Mol. Biol."},{"issue":"1","key":"2026090306572924500_cit23","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1017\/S0033583511000102","article-title":"Electron tomography\nof cells","volume":"45","author":"Gan","year":"2012","journal-title":"Q. Rev. Biophys."},{"key":"2026090306572924500_cit24","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.1002\/pro.3858","article-title":"Three-dimensional\norganization of\nthe cytoskeleton: a cryo-electron tomography perspective","volume":"29","author":"Chakraborty","year":"2020","journal-title":"Protein Sci."},{"key":"2026090306572924500_cit25","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/bs.mcb.2019.05.001","article-title":"Content-aware image\nrestoration for electron microscopy","volume":"152","author":"Buchholz","year":"2019","journal-title":"Methods\nCell Biol."},{"key":"2026090306572924500_cit26","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1109\/ISBI.2019.8759519","article-title":"Cryo-CARE: content-aware image\nrestoration for cryo-transmission electron microscopy data","volume-title":"2019 IEEE 16th International Symposium on Biomedical\nImaging (ISBI 2019)","author":"Buchholz","year":"2019"},{"key":"2026090306572924500_cit27","doi-asserted-by":"publisher","first-page":"100068","DOI":"10.1016\/j.yjsbx.2022.100068","article-title":"AreTomo:\nAn integrated software package for automated marker-free, motion-corrected\ncryo-electron tomographic alignment and reconstruction","volume":"6","author":"Zheng","year":"2022","journal-title":"J. Struct. Biol. X"},{"issue":"4","key":"2026090306572924500_cit28","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1038\/nmeth.4193","article-title":"MotionCor2: anisotropic correction of beam-induced\nmotion for improved cryo-electron microscopy","volume":"14","author":"Zheng","year":"2017","journal-title":"Nat. Methods"},{"issue":"1","key":"2026090306572924500_cit29","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1006\/jsbi.1996.0013","article-title":"Computer visualization of three-dimensional\nimage data using IMOD","volume":"116","author":"Kremer","year":"1996","journal-title":"J. Struct. Biol."},{"key":"2026090306572924500_cit30","doi-asserted-by":"publisher","DOI":"10.3791\/64435","article-title":"Deep learning-based segmentation of cryo-electron tomograms","volume":"189","author":"Heebner","year":"2022","journal-title":"J. Vis. Exp."},{"issue":"8","key":"2026090306572924500_cit31","doi-asserted-by":"publisher","first-page":"2135","DOI":"10.1016\/j.cell.2021.02.047","article-title":"The molecular basis\nfor sarcomere organization in vertebrate\nskeletal muscle","volume":"184","author":"Wang","year":"2021","journal-title":"Cell"},{"issue":"6582","key":"2026090306572924500_cit32","doi-asserted-by":"publisher","DOI":"10.1126\/science.abn1934","article-title":"Structures\nfrom intact myofibrils\nreveal mechanism of thin filament regulation through nebulin","volume":"375","author":"Wang","year":"2022","journal-title":"Science"},{"key":"2026090306572924500_cit33","doi-asserted-by":"publisher","first-page":"863","DOI":"10.1038\/s41586-023-06690-5","article-title":"Structure of the native myosin filament\nin the relaxed cardiac sarcomere","volume":"623","author":"Tamborrini","year":"2023","journal-title":"Nature"},{"key":"2026090306572924500_cit34","doi-asserted-by":"publisher","DOI":"10.1101\/2023.10.26.564098","article-title":"Cryo-electron tomography reveals the\nstructural diversity of cardiac proteins in their cellular context","author":"Woldeyes","journal-title":"bioRxiv"},{"issue":"41","key":"2026090306572924500_cit35","doi-asserted-by":"publisher","first-page":"12705","DOI":"10.1073\/pnas.1508073112","article-title":"Contractility of\nsingle cardiomyocytes differentiated from pluripotent stem cells depends\non physiological shape and substrate stiffness","volume":"112","author":"Ribeiro","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"4","key":"2026090306572924500_cit36","doi-asserted-by":"publisher","first-page":"H1313","DOI":"10.1152\/ajpheart.00816.2005","article-title":"Mechanical\nstress-strain sensors embedded in cardiac cytoskeleton: Z disk, titin,\nand associated structures","volume":"290","author":"Hoshijima","year":"2006","journal-title":"Am. J. Physiol. Heart\nCirc. Physiol."},{"issue":"31","key":"2026090306572924500_cit37","doi-asserted-by":"publisher","first-page":"12607","DOI":"10.1073\/pnas.1309493110","article-title":"Molecular\nconsequences of the R453C hypertrophic cardiomyopathy mutation on\nhuman \u03b2-cardiac myosin motor function","volume":"110","author":"Sommese","year":"2013","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"11","key":"2026090306572924500_cit38","doi-asserted-by":"publisher","first-page":"2857","DOI":"10.1016\/j.celrep.2016.11.040","article-title":"Early-onset hypertrophic\ncardiomyopathy\nmutations significantly increase the velocity, force, and actin-activated\nATPase activity of human \u03b2-cardiac myosin","volume":"17","author":"Adhikari","year":"2016","journal-title":"Cell Rep."},{"issue":"1","key":"2026090306572924500_cit39","doi-asserted-by":"publisher","first-page":"4019","DOI":"10.1038\/s41467-018-06191-4","article-title":"Hypertrophic\ncardiomyopathy disease\nresults from disparate impairments of cardiac myosin function and\nauto-inhibition","volume":"9","author":"Robert-Paganin","year":"2018","journal-title":"Nat. Commun."},{"issue":"52","key":"2026090306572924500_cit40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2521561122","article-title":"A myosin hypertrophic\ncardiomyopathy\nmutation disrupts the super-relaxed state and boosts contractility\nby enhanced actin attachment","volume":"122","author":"Cail","year":"2025","journal-title":"Proc. Natl. Acad.\nSci. U. S. A."},{"issue":"1","key":"2026090306572924500_cit41","doi-asserted-by":"publisher","first-page":"8751","DOI":"10.1038\/s41467-025-63816-1","article-title":"Hypertrophic cardiomyopathy mutations Y115H and E497D\ndisrupt the folded-back state of human \u03b2-cardiac\nmyosin allosterically","volume":"16","author":"Nandwani","year":"2025","journal-title":"Nat. Commun."},{"issue":"1","key":"2026090306572924500_cit42","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.athoracsur.2007.07.063","article-title":"Current\neffectiveness and risks of isolated septal myectomy for hypertrophic\nobstructive cardiomyopathy","volume":"85","author":"Smedira","year":"2008","journal-title":"Ann. Thoracic Surg."},{"issue":"3","key":"2026090306572924500_cit43","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1016\/j.jacc.2005.02.090","article-title":"Long-term effects of surgical septal myectomy\non survival in patients with obstructive hypertrophic cardiomyopathy","volume":"46","author":"Ommen","year":"2005","journal-title":"J. Am. College Cardiol."},{"issue":"35","key":"2026090306572924500_cit44","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1809540115","article-title":"Deciphering the super relaxed state of human \u03b2-cardiac myosin and the mode of action of mavacamten\nfrom myosin molecules to muscle fibers","volume":"115","author":"Anderson","year":"2018","journal-title":"Proc.\nNatl. Acad. Sci. U. S. A."},{"key":"2026090306572924500_cit45","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1016\/j.yjmcc.2018.01.012","article-title":"Localization of transcripts, translation, and degradation for spatiotemporal\nsarcomere maintenance","volume":"116","author":"Lewis","year":"2018","journal-title":"J. Mol. Cell. Cardiol."},{"key":"2026090306572924500_cit46","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.yjmcc.2020.08.018","article-title":"Under construction:\nThe dynamic assembly, maintenance,\nand degradation of the cardiac sarcomere","volume":"148","author":"Martin","year":"2020","journal-title":"J.\nMol. Cell. Cardiol."},{"key":"2026090306572924500_cit47","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.ijcard.2021.09.009","article-title":"Stress\nactivated signalling\nimpaired protein quality control pathways in human hypertrophic cardiomyopathy","volume":"344","author":"Hassoun","year":"2021","journal-title":"Int. J. Cardiol."},{"issue":"6215","key":"2026090306572924500_cit48","doi-asserted-by":"publisher","first-page":"1377","DOI":"10.1126\/science.1259530","article-title":"Ultrastable gold substrates for electron\ncryomicroscopy","volume":"346","author":"Russo","year":"2014","journal-title":"Science"},{"issue":"11","key":"2026090306572924500_cit49","doi-asserted-by":"publisher","first-page":"115018","DOI":"10.1088\/1361-6439\/ab419a","article-title":"Extracellular\nmatrix micropatterning technology for whole cell cryogenic electron\nmicroscopy studies","volume":"29","author":"Engel","year":"2019","journal-title":"J. Micromech. Microeng."},{"issue":"4","key":"2026090306572924500_cit50","doi-asserted-by":"publisher","first-page":"107791","DOI":"10.1016\/j.jsb.2021.107791","article-title":"Lattice\nmicropatterning for cryo-electron\ntomography studies of cell-cell contacts","volume":"213","author":"Engel","year":"2021","journal-title":"J.\nStruct. Biol."},{"key":"2026090306572924500_cit51","doi-asserted-by":"publisher","DOI":"10.17504\/protocols.io.bz22p8ge","article-title":"Micropatterning\nEM grids for\ncryo-electron tomography of cells","author":"Engel","year":"2022","journal-title":"protocols.io"},{"issue":"1","key":"2026090306572924500_cit52","doi-asserted-by":"publisher","first-page":"9802","DOI":"10.1038\/s41467-025-64795-z","article-title":"Extracellular\nfilaments revealed by affinity capture cryogenic-electron tomography","volume":"16","author":"Engel","year":"2025","journal-title":"Nat. Commun."},{"key":"2026090306572924500_cit53","doi-asserted-by":"publisher","first-page":"860","DOI":"10.1107\/S205225252000929X","article-title":"Electron-event\nrepresentation data enable efficient\ncryoEM file storage with full preservation of spatial and temporal\nresolution","volume":"7","author":"Guo","year":"2020","journal-title":"IUCrJ"},{"key":"2026090306572924500_cit54","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.01456","article-title":"Collaboration gets\nthe most out of software","volume":"2","author":"Morin","year":"2013","journal-title":"eLife"}],"container-title":["ACS Nano"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsnano.6c03369","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/20\/34\/23748\/66809783\/acsnano.6c03369.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/20\/34\/23748\/66809783\/acsnano.6c03369.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:57:41Z","timestamp":1788433061000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancac3\/article\/20\/34\/23748\/5272743\/Cryo-Electron-Tomography-Reveals-Nanoscale-Thick"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,18]]},"references-count":54,"journal-issue":{"issue":"34","published-online":{"date-parts":[[2026,8,18]]},"published-print":{"date-parts":[[2026,9,1]]}},"URL":"https:\/\/doi.org\/10.1021\/acsnano.6c03369","relation":{},"ISSN":["1936-0851","1936-086X"],"issn-type":[{"value":"1936-0851","type":"print"},{"value":"1936-086X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,18]]}}}