{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T17:26:17Z","timestamp":1787073977316,"version":"build-2736575974"},"reference-count":37,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100016349","name":"Sichuan Normal University","doi-asserted-by":"publisher","award":["KY2025014"],"award-info":[{"award-number":["KY2025014"]}],"id":[{"id":"10.13039\/501100016349","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010855","name":"Jiangsu Provincial Department of Human Resources and Social Security","doi-asserted-by":"publisher","award":["2025ZB395"],"award-info":[{"award-number":["2025ZB395"]}],"id":[{"id":"10.13039\/501100010855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China (NSFC)","doi-asserted-by":"crossref","award":["22404119"],"award-info":[{"award-number":["22404119"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China (NSFC)","doi-asserted-by":"crossref","award":["32501984"],"award-info":[{"award-number":["32501984"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"crossref","award":["2024NSFSC1129"],"award-info":[{"award-number":["2024NSFSC1129"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Laccase is an environmentally friendly catalyst with water as the sole catalytic byproduct, yet its biomedical detection potential remains underexplored. Herein, a ligand engineering strategy was employed to synthesize Mn-cysteine nanoflowers (Mn-Cys NF) with laccase-mimicking activity via a one-pot method, using manganese (Mn) with rich valence variations as the active center and cysteine (Cys) as the ligand. Spectroscopic characterizations confirmed Cys-modulated Mn electronic structure, and theoretical calculations validated enhanced substrate adsorption and reduced reaction barriers. The specific activity of Mn-Cys NF is approximately 3.56 times that of natural laccase and exhibited excellent stability across pH, temperature, ionic strength, and organic solvent conditions. Leveraging this high-performance nanozyme, a CRISPR\/Cas12a electrochemical biosensor was constructed with a DNA triangular prism interface, where a target-triggered catalytic hairpin assembly (CHA)\u2013DNAzyme cascade regulated Cas12a cleavage to enable signal-on detection. This biosensor achieved quantification of hepatocellular carcinoma (HCC) biomarkers alpha-fetoprotein (AFP) and microRNA-122 (miRNA-122), with detection limits as low as 4.47 fg\/mL and 6.21 aM, respectively. It also effectively discriminated HCC patients from healthy individuals in clinical serum samples. This work offers a ligand engineering strategy for designing high-performance laccase-mimicking nanozymes and expands the application scope of laccase nanozymes from environmental remediation to biomedical biosensing.<\/jats:p>","DOI":"10.1021\/acsnano.6c07904","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T15:01:49Z","timestamp":1786028509000},"page":"22787-22803","source":"Crossref","is-referenced-by-count":0,"title":["Ligand-Engineered\nMn-Cysteine as a Potent Laccase\nMimic for CRISPR\/Cas12a Electrochemical Biosensing of Hepatocellular\nCarcinoma Biomarkers"],"prefix":"10.1021","volume":"20","author":[{"given":"Ya","family":"Zhou","sequence":"first","affiliation":[{"name":"Sichuan Normal University , , ,","place":["Chengdu, China, 610066"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Li","sequence":"additional","affiliation":[{"name":"Sichuan Normal University , , ,","place":["Chengdu, China, 610066"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjie","family":"Chen","sequence":"additional","affiliation":[{"name":"Sichuan Normal University , , ,","place":["Chengdu, China, 610066"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0234-5395","authenticated-orcid":true,"given":"Jing","family":"Ye","sequence":"additional","affiliation":[{"name":"Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences , , ,","place":["Changchun, China, 130102"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Yan","sequence":"additional","affiliation":[{"name":"Sichuan Normal University , , ,","place":["Chengdu, China, 610066"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Yang","sequence":"additional","affiliation":[{"name":"Sichuan Normal University , , ,","place":["Chengdu, China, 610066"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Meng","sequence":"additional","affiliation":[{"name":"College of Energy, Soochow University , ,","place":["Suzhou, China, 215006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongxu","family":"Jiao","sequence":"additional","affiliation":[{"name":"Northeast Forestry University , , ,","place":["Harbin, China, 150040"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dewen","family":"Wang","sequence":"additional","affiliation":[{"name":"Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , , ,","place":["Changchun, China, 130022"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6482-9286","authenticated-orcid":true,"given":"Liping","family":"Zhu","sequence":"additional","affiliation":[{"name":"Sichuan Normal University , , ,","place":["Chengdu, China, 610066"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0021-5135","authenticated-orcid":true,"given":"Xiurong","family":"Yang","sequence":"additional","affiliation":[{"name":"Changchun Institute of Applied Chemistry, Chinese Academy of Sciences , , ,","place":["Changchun, China, 130022"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"2026081810305385500_cit1","doi-asserted-by":"publisher","first-page":"1440","DOI":"10.1038\/s41467-018-03903-8","article-title":"In Vivo\nGuiding Nitrogen-Doped Carbon\nNanozyme for Tumor Catalytic Therapy","volume":"9","author":"Fan","year":"2018","journal-title":"Nat. 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