{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:02:24Z","timestamp":1789581744200,"version":"build-2803163510"},"reference-count":81,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":15,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R35CA263815"],"award-info":[{"award-number":["R35CA263815"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001315","name":"Sid W. 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To address this limitation, we developed a microfluidic electrophoresis (MEP) device for the label-free, charge-based enrichment of EVs from the serum of patients with pancreatic cancer (PaCa). We first validated the principle of charge-based cancer EV enrichment using cell-line-derived EVs, demonstrating that increased signaling through mutant Kirsten rat sarcoma viral oncogene homologue (KRAS), particularly KrasG12D, was associated with enhanced EV secretion and a shift toward more negative \u03b6-potential values. We then evaluated EVs derived from the serum of patients with PaCa to assess the clinical relevance and translational potential of the MEP platform. Further analyses identified phosphatidylserine and extraluminal DNA as part of the molecular determinants of the enhanced anionic nature of the PaCa-derived EVs. Overall, this proof-of-concept study introduces a promising microfluidic platform for enriching circulating cancer EVs with potential applications in the rapid detection of PaCa.<\/jats:p>","DOI":"10.1021\/acsnano.6c08263","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T09:57:39Z","timestamp":1788429459000},"page":"24605-24620","source":"Crossref","is-referenced-by-count":0,"title":["Oncogenic\nKRAS Regulates Secretion of Extracellular\nVesicles and Surface Membrane Charge via Regulation of Phosphatidylserine\nin Pancreatic Cancer"],"prefix":"10.1021","volume":"20","author":[{"given":"Paul A.","family":"Spezza","sequence":"first","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center , , , ,","place":["Houston, Texas, United States, 77030"]},{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9079-7373","authenticated-orcid":true,"given":"Kshipra S.","family":"Kapoor","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center , , , ,","place":["Houston, Texas, United States, 77030"]},{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrice P.","family":"Pforr","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center , , , ,","place":["Houston, Texas, United States, 77030"]},{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3100-3847","authenticated-orcid":true,"given":"Xin","family":"Luo","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson 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77005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6173-388X","authenticated-orcid":true,"given":"Seoyun","family":"Kong","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center , , , ,","place":["Houston, Texas, United States, 77030"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V.","family":"Ramirez","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center , , , ,","place":["Houston, Texas, United States, 77030"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda G.","family":"Kugeratski","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center , , , ,","place":["Houston, Texas, United States, 77030"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0610-835X","authenticated-orcid":true,"given":"Sibani Lisa","family":"Biswal","sequence":"additional","affiliation":[{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4092-4991","authenticated-orcid":true,"given":"Kathleen M.","family":"McAndrews","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center , , , ,","place":["Houston, Texas, United States, 77030"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Gebauer","sequence":"additional","affiliation":[{"name":"University of Witten\/Herdecke, Helios University Hospital Wuppertal , , ,","place":["Wuppertal, Germany, 42283"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Kahlert","sequence":"additional","affiliation":[{"name":"Heidelberg University, Heidelberg University Hospital , , ,","place":["Heidelberg, Germany, 69120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3509-3054","authenticated-orcid":true,"given":"Jacob T.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]},{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]},{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]},{"name":"Baylor College of Medicine , , , ,","place":["Houston, Texas, United States, 77030"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2190-547X","authenticated-orcid":true,"given":"Raghu","family":"Kalluri","sequence":"additional","affiliation":[{"name":"The University of Texas MD Anderson Cancer Center , , , ,","place":["Houston, Texas, United States, 77030"]},{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]},{"name":"Rice University , , , ,","place":["Houston, Texas, United States, 77005"]},{"name":"The University of Texas Medical Branch , , , ,","place":["Galveston, Texas, United States, 77555"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"issue":"22","key":"2026091613064994700_cit1","doi-asserted-by":"publisher","first-page":"2112","DOI":"10.1056\/NEJMoa2119662","article-title":"Neoantigen T-Cell Receptor\nGene Therapy in Pancreatic 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