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However, most EV isolation methods often require large sample volumes and specialized instruments or involve trade-offs between purity, yield, cost, and scalability. We developed MagPEG, a workflow that combines poly(ethylene glycol) (PEG)-mediated EV aggregation with magnetic beads to provide a simple, reproducible alternative to ultracentrifugation, size-exclusion chromatography, and commercial precipitation kits. Our optimization experiments clarified the PEG concentration, ionic strength, and bead surface chemistry that collectively influence EV aggregation, capture efficiency, and contaminant coprecipitation, allowing us to define conditions that improve purity while maintaining high recovery. Compared with commonly used methods, MagPEG produced EVs with comparable size distribution, EV markers, and proteomic profiles while relying only on standard laboratory supplies. A key feature of the platform is that EVs and EV-associated DNA, RNA, and proteins can be sequentially extracted from the same bead-bound material, reducing sample loss and hands-on time and enabling multiomic analysis for limited clinical or small animal samples. MagPEG is compatible with downstream applications including proteomics, bead-based assays, and miRNA quantification. When applied to human serum, the method supported high-throughput EV proteomic profiling and enabled the identification of Alzheimer\u2019s disease-associated protein signatures, illustrating its utility for biomarker discovery. Overall, our results establish MagPEG as a powerful, rapid, scalable, and high-throughput solution for translational applications in biomarker discovery.<\/jats:p>","DOI":"10.1021\/acsnano.6c09351","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T12:36:18Z","timestamp":1788266178000},"page":"24492-24511","source":"Crossref","is-referenced-by-count":0,"title":["A\nRapid Poly(ethylene glycol)-Assisted Magnetic Isolation\nApproach for High-Throughput Extracellular Vesicle Isolation and Subsequent\nBiomarker Analysis"],"prefix":"10.1021","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8228-7487","authenticated-orcid":true,"given":"Li","family":"Sun","sequence":"first","affiliation":[{"name":"Florida State University College of Medicine , , , ,","place":["Tallahassee, Florida, United States, 32306"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leanne","family":"Duke","sequence":"additional","affiliation":[{"name":"Florida State University College of Medicine , , , ,","place":["Tallahassee, Florida, United States, 32306"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Vied","sequence":"additional","affiliation":[{"name":"Florida State University , , , ,","place":["Tallahassee, Florida, United States, 32306"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grace","family":"Hammel","sequence":"additional","affiliation":[{"name":"Florida State University College of Medicine , , , ,","place":["Tallahassee, Florida, United States, 32306"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brandon","family":"Pate","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Wang","sequence":"additional","affiliation":[{"name":"Florida State University College of Medicine , , , ,","place":["Tallahassee, Florida, United States, 32306"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dai-Shi","family":"Tian","sequence":"additional","affiliation":[{"name":"Huazhong University of Science and Technology , , ,","place":["Wuhan, China, 430030"]},{"name":"Huazhong University of Science and Technology , , ,","place":["Wuhan, China, 430030"]},{"name":"Huazhong University of Science and Technology , , ,","place":["Wuhan, China, 430030"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianqing","family":"Fan","sequence":"additional","affiliation":[{"name":"Princeton University , , , ,","place":["Princeton, New Jersey, United States, 08544"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0176-493X","authenticated-orcid":true,"given":"Yi","family":"Ren","sequence":"additional","affiliation":[{"name":"Florida State University College of Medicine , , , ,","place":["Tallahassee, Florida, United States, 32306"]},{"name":"Guangzhou Medical University , , ,","place":["Guangzhou, China, 511436"]},{"name":"The First Affiliated Hospital of Guangzhou Medical University , , ,","place":["Guangzhou, China, 511436"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,26]]},"reference":[{"issue":"5","key":"2026090809091015400_cit1","doi-asserted-by":"publisher","first-page":"359","DOI":"10.3390\/biology10050359","article-title":"Extracellular\nVesicles as Biomarkers\nand Therapeutic Tools: From Pre-Clinical to Clinical Applications","volume":"10","author":"Ciferri","year":"2021","journal-title":"Biology (Basel)"},{"issue":"1","key":"2026090809091015400_cit2","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1038\/s41392-024-01735-1","article-title":"Extracellular vesicles as tools and targets in therapy\nfor diseases","volume":"9","author":"Kumar","year":"2024","journal-title":"Signal Transduction and Targeted\nTherapy"},{"issue":"3\u20134","key":"2026090809091015400_cit3","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1002\/prca.201400114","article-title":"Exosomes in bodily fluids are a highly stable resource\nof disease biomarkers","volume":"9","author":"Boukouris","year":"2015","journal-title":"Proteomics Clin Appl."},{"issue":"1","key":"2026090809091015400_cit4","doi-asserted-by":"publisher","first-page":"12377","DOI":"10.1038\/s41598-018-30786-y","article-title":"Extracellular vesicles protect glucuronidase model\nenzymes during freeze-drying","volume":"8","author":"Frank","year":"2018","journal-title":"Sci. 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