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Here, we develop a bispecific immune-bridging platform (NVMB) to address these limitations. NVMB consists of tumor cell membrane-derived nanovesicles enriched with damage-associated molecular patterns (DAMPs) that activate DCs and support T-cell responses. The vesicle surface is further functionalized with two aptamers targeting DCs and CD8+ T cells, respectively, thereby enhancing their spatial proximity and facilitating functional DC\u2013T cell interactions. This biomimetic design combines colloidal stability with programmable surface functionalization. Mechanistically, NVMB promotes DC\u2013T interactions and enhances activation-associated phenotypes in both cell populations. In multiple immunocompetent mouse tumor models, NVMB suppresses tumor growth, increases intratumoral accumulation of mature DCs and activated CD8+ T cells, and reshapes the tumor microenvironment transcriptome toward enhanced antitumor immune responses. Moreover, combination with immune checkpoint blockade (ICB) further improves antitumor efficacy compared with either monotherapy, supporting the potential of NVMB as a complementary strategy for enhancing ICB responses. In patient-derived cocultures of circulating tumor cells (CTCs) and autologous peripheral blood mononuclear cells (PBMCs), NVMB enhances T-cell-mediated clearance of CTCs. These findings support NVMB as a stable and modular nanoplatform that integrates immunogenic signaling with immune-cell spatial coordination, providing a promising strategy for strengthening antitumor immunity and improving the efficacy of existing immunotherapies.<\/jats:p>","DOI":"10.1021\/acsnano.6c11994","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:02:18Z","timestamp":1791460938000},"page":"27890-27907","source":"Crossref","is-referenced-by-count":0,"title":["A Bispecific Nanobridge\nDerived from Immunogenic Tumor\nMembranes Restores Dendritic Cell\u2013T Cell Crosstalk for Enhanced\nSolid Tumor Immunotherapy"],"prefix":"10.1021","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8206-3487","authenticated-orcid":true,"given":"Xueqian","family":"Li","sequence":"first","affiliation":[{"name":"Anhui Medical University , , ,","place":["Hefei, P. R. China, 230011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Qiao","sequence":"additional","affiliation":[{"name":"Anhui Normal University , , ,","place":["Wuhu, P. R. China, 241000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanrong","family":"Wang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Anhui Medical University , , ,","place":["Hefei, P. R. China, 230022"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Xiong","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , ,","place":["Hefei, P. R. China, 230011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Zhang","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , ,","place":["Hefei, P. R. China, 230011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guisong","family":"Shan","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , ,","place":["Hefei, P. R. China, 230011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuting","family":"Sun","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , ,","place":["Hefei, P. R. China, 230011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Ding","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , ,","place":["Hefei, P. R. China, 230011"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2086-563X","authenticated-orcid":true,"given":"Xiaoyan","family":"He","sequence":"additional","affiliation":[{"name":"Anhui Medical University , , ,","place":["Hefei, P. R. 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Commun."},{"issue":"6","key":"2026102008171298300_cit3","doi-asserted-by":"publisher","first-page":"982","DOI":"10.1016\/j.immuni.2022.04.016","article-title":"Dendritic Cells Can\nPrime Anti-Tumor CD8+ T Cell Responses\nthrough Major Histocompatibility Complex Cross-Dressing","volume":"55","author":"MacNabb","year":"2022","journal-title":"Immunity"},{"key":"2026102008171298300_cit4","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1038\/s41590-022-01132-2","article-title":"Immunogenic\nCell Stress\nand Death","volume":"23","author":"Kroemer","year":"2022","journal-title":"Nat. Immunol."},{"key":"2026102008171298300_cit5","doi-asserted-by":"publisher","first-page":"4066","DOI":"10.1038\/s41467-023-39920-5","article-title":"ARIH1\nActivates STING-Mediated T-Cell Activation and Sensitizes Tumors\nto Immune Checkpoint Blockade","volume":"14","author":"Liu","year":"2023","journal-title":"Nat. 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Immunother Cancer"},{"key":"2026102008171298300_cit26","doi-asserted-by":"publisher","DOI":"10.1126\/science.adq1405","article-title":"A Distinct Priming Phase\nRegulates CD8 T Cell\nImmunity by Orchestrating Paracrine Il-2 Signals","volume":"388","author":"Jobin","year":"2025","journal-title":"Science"},{"key":"2026102008171298300_cit27","doi-asserted-by":"publisher","first-page":"2087","DOI":"10.1038\/s41467-023-37825-x","article-title":"Expansion\nof Circulating Stem-Like CD8+ T\nCells by Adding Cd122-Directed Il-2 Complexes to Radiation and Anti-Pd1\nTherapies in Mice","volume":"14","author":"Onyshchenko","year":"2023","journal-title":"Nat. Commun."},{"key":"2026102008171298300_cit28","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1038\/s43018-023-00668-y","article-title":"Cytokine-Armed Dendritic Cell Progenitors for Antigen-Agnostic\nCancer Immunotherapy","volume":"5","author":"Ghasemi","year":"2024","journal-title":"Nat. Cancer"},{"key":"2026102008171298300_cit29","doi-asserted-by":"publisher","first-page":"4755","DOI":"10.1038\/s41467-021-24902-2","article-title":"Synergistic Effect of\nTumor Chemo-Immunotherapy Induced by Leukocyte-Hitchhiking\nThermal-Sensitive Micelles","volume":"12","author":"Qi","year":"2021","journal-title":"Nat. Commun."},{"key":"2026102008171298300_cit30","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1158\/2326-6066.CIR-20-0785","article-title":"Site-Dependent\nImmune Escape Due\nto Impaired Dendritic Cell Cross-Priming","volume":"9","author":"Diamond","year":"2021","journal-title":"Cancer\nImmunol. Res."},{"key":"2026102008171298300_cit31","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.1038\/s43018-021-00247-z","article-title":"Low Neoantigen Expression\nand Poor T-Cell Priming Underlie Early Immune Escape in Colorectal\nCancer","volume":"2","author":"Westcott","year":"2021","journal-title":"Nat. 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Res."},{"key":"2026102008171298300_cit34","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1038\/s41586-022-04735-9","article-title":"Neoantigen\nQuality Predicts Immunoediting in Survivors\nof Pancreatic Cancer","volume":"606","author":"Luksza","year":"2022","journal-title":"Nature"},{"key":"2026102008171298300_cit35","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1038\/s41591-018-0014-x","article-title":"Understanding the\nTumor Immune Microenvironment (Time) for Effective Therapy","volume":"24","author":"Binnewies","year":"2018","journal-title":"Nat. Med."},{"issue":"6","key":"2026102008171298300_cit36","doi-asserted-by":"publisher","first-page":"1389","DOI":"10.1038\/s41591-023-02345-0","article-title":"Intratumoral dendritic\ncell\u2013CD4+ T helper cell\nniches enable CD8+ T cell differentiation following PD-1 blockade\nin hepatocellular carcinoma","volume":"29","author":"Magen","year":"2023","journal-title":"Nat. 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Sci. 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