{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T12:49:32Z","timestamp":1792500572597,"version":"4.5.0"},"reference-count":37,"publisher":"American Chemical Society (ACS)","issue":"41","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100003347","name":"Fudan University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YFA0923503"],"award-info":[{"award-number":["2025YFA0923503"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52322305"],"award-info":[{"award-number":["52322305"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52473098"],"award-info":[{"award-number":["52473098"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["T2321003"],"award-info":[{"award-number":["T2321003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["T2596012"],"award-info":[{"award-number":["T2596012"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Xiaomi Young Scholar Program"},{"name":"Ruiqing Young Scholar Program"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Spatial organization underpins efficient multistep catalysis in nature, yet recreating such organization on living cell surfaces using mild, enzyme-compatible and catalytically functional materials remains challenging. Here we report an l-cysteine-triggered silk fibroin\/lysozyme ultrathin film (SLF) that assembles directly on living microbial cells as a catalytic surface layer. SLF forms continuous nanoscale coatings on Saccharomyces cerevisiae, Gluconobacter oxydans and Escherichia coli Nissle 1917. Spectroscopic and structural analyses show that film formation proceeds through electrostatic complexation, restricted conformational rearrangement, increased hydrophobic exposure, \u03b2-sheet-rich ordering and network formation, rather than simple deposition. The coating maintains cell viability, enhances stress tolerance and provides size-selective permeability for small-molecule transport. SLF also incorporates exogenous enzymes efficiently during assembly. As a proof of concept, \u03c9-transaminase-integrated SLF on G. oxydans couples host glycerol oxidation with surface transamination, enabling one-pot conversion of glycerol to serinol with a titer of 2.7 g L\u20131 within 12 h, up to 37-fold higher than a stepwise process. This work establishes ultrathin protein films as living catalytic interfaces for single-cell biocatalyst design and cascade biomanufacturing.<\/jats:p>","DOI":"10.1021\/acsnano.6c12754","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T10:01:56Z","timestamp":1791540116000},"page":"27949-27959","source":"Crossref","is-referenced-by-count":0,"title":["Ultrathin Protein\nFilms Create Living Catalytic Interfaces\non Microbial Cells"],"prefix":"10.1021","volume":"20","author":[{"given":"Mindi","family":"Ming","sequence":"first","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menglin","family":"Xiao","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 201102"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Xu","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gangfeng","family":"Tang","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Ren","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianliang","family":"Miao","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0868-2934","authenticated-orcid":true,"given":"Jinrong","family":"Yao","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7706-4166","authenticated-orcid":true,"given":"Xin","family":"Chen","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]},{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5334-4008","authenticated-orcid":true,"given":"Zhengzhong","family":"Shao","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]},{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1156-0479","authenticated-orcid":true,"given":"Shengjie","family":"Ling","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]},{"name":"Fudan University , , ,","place":["Shanghai, China, 200433"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"2026102008164009600_cit1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adu2454","article-title":"Computational Design of Serine Hydrolases","volume":"388","author":"Lauko","year":"2025","journal-title":"Science"},{"issue":"4","key":"2026102008164009600_cit2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202416556","article-title":"A Supramolecular\nApproach to Engineering Living Cells with Enzymes for Adaptive and\nRecyclable Cascade Synthesis","volume":"64","author":"Wang","year":"2025","journal-title":"Angew. 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Protoc."},{"issue":"37","key":"2026102008164009600_cit25","doi-asserted-by":"publisher","first-page":"33164","DOI":"10.1021\/acsnano.5c06669","article-title":"All-Water-Based\nNanofabrication of Multilayer Biopolymer\/Inorganic Reflectors with\nReconfigurable Structural Color Patterns","volume":"19","author":"Kim","year":"2025","journal-title":"ACS\nNano"},{"issue":"16","key":"2026102008164009600_cit26","doi-asserted-by":"publisher","first-page":"2312324","DOI":"10.1002\/adfm.202312324","article-title":"An Interfacial-Assembled Self-Supporting\nNanofilm Induced by Strong Intermolecular Interaction between Silk\nFibroin and Lysozyme","volume":"34","author":"Miao","year":"2024","journal-title":"Adv. Funct. Mater."},{"key":"2026102008164009600_cit27","doi-asserted-by":"publisher","first-page":"102908","DOI":"10.1016\/j.mtbio.2026.102908","article-title":"Modulating\nCell Surface\nChemistry through Mild Reduction Reinforces Extracellular-to-Intracellular\nTransmission Forces and Mechano-Signaling","volume":"37","author":"Jeong","year":"2026","journal-title":"Mater.\nToday Bio"},{"issue":"2","key":"2026102008164009600_cit28","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202500914","article-title":"Cell Surface\nThiol Engineering Mechanoregulates\nMyogenic Differentiation via the FAK\u2013PI3K\u2013AKT Axis","volume":"15","author":"Kim","year":"2026","journal-title":"Adv. Healthcare Mater."},{"issue":"7","key":"2026102008164009600_cit29","doi-asserted-by":"publisher","first-page":"2850","DOI":"10.1002\/anie.201912848","article-title":"The Synthesis of a 2D Ultra-Large Protein Supramolecular Nanofilm\nby Chemoselective Thiol\u2013Disulfide Exchange and Its Emergent\nFunctions","volume":"59","author":"Xu","year":"2020","journal-title":"Angew. Chem., Int. Ed."},{"issue":"18","key":"2026102008164009600_cit30","doi-asserted-by":"publisher","first-page":"3128","DOI":"10.1002\/smll.201202992","article-title":"Cell Surface\nEngineering with Edible\nProtein Nanoshells","volume":"9","author":"Drachuk","year":"2013","journal-title":"Small"},{"issue":"5","key":"2026102008164009600_cit31","doi-asserted-by":"publisher","first-page":"1552","DOI":"10.1073\/pnas.56.5.1552","article-title":"Protein Conformations in Cellular\nMembranes","volume":"56","author":"Wallach","year":"1966","journal-title":"Proc. Natl. Acad. Sci. 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Basic Microbiol."},{"issue":"1","key":"2026102008164009600_cit33","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.fm.2011.10.010","article-title":"Effect of Lysozyme\non \u201cFlor\u201d Velum Yeasts in the Biological Aging of Sherry\nWines","volume":"30","author":"Rolda\u0301n","year":"2012","journal-title":"Food Microbiol."},{"issue":"5","key":"2026102008164009600_cit34","doi-asserted-by":"publisher","first-page":"3673","DOI":"10.1128\/AEM.72.5.3673-3677.2006","article-title":"Effects of Quaternary Ammonium Silane\nCoatings on Mixed Fungal and Bacterial Biofilms on Tracheoesophageal\nShunt Prostheses","volume":"72","author":"Oosterhof","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"issue":"6","key":"2026102008164009600_cit35","doi-asserted-by":"publisher","first-page":"2378","DOI":"10.1039\/D2GC04566D","article-title":"Cascading and Precise Regulation\nof the Selective Bio-Production of 2- or 5-Ketogluconic Acid from\nGlucose with Whole-Cell Catalysis Technology","volume":"25","author":"Hua","year":"2023","journal-title":"Green Chem."},{"issue":"32","key":"2026102008164009600_cit36","doi-asserted-by":"publisher","first-page":"10809","DOI":"10.1021\/acssuschemeng.1c02511","article-title":"Coculture of Gluconobacter oxydans and Escherichia coli for 3,4-Dihydroxybutyric Acid\nProduction from Xylose","volume":"9","author":"Zhang","year":"2021","journal-title":"ACS Sustainable Chem.\nEng."},{"issue":"3","key":"2026102008164009600_cit37","doi-asserted-by":"publisher","first-page":"1140","DOI":"10.1039\/D0GC03918G","article-title":"One-Pot Biotransformation\nof Glycerol into Serinol\nCatalysed by Biocatalytic Composites Made of Whole Cells and Immobilised\nEnzymes","volume":"23","author":"Ripoll","year":"2021","journal-title":"Green Chem."}],"container-title":["ACS Nano"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsnano.6c12754","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/20\/41\/27949\/68796570\/acsnano.6c12754.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/20\/41\/27949\/68796570\/acsnano.6c12754.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T12:30:45Z","timestamp":1792499445000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancac3\/article\/20\/41\/27949\/5447583\/Ultrathin-Protein-Films-Create-Living-Catalytic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,9]]},"references-count":37,"journal-issue":{"issue":"41","published-online":{"date-parts":[[2026,10,9]]},"published-print":{"date-parts":[[2026,10,20]]}},"URL":"https:\/\/doi.org\/10.1021\/acsnano.6c12754","relation":{},"ISSN":["1936-0851","1936-086X"],"issn-type":[{"value":"1936-0851","type":"print"},{"value":"1936-086X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,9]]}}}