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Super-resolution microscopy was recently proposed as an effective tool to unveil one-dimensional nanoscale structures in aqueous media upon chemical functionalization with suitable fluorescent probes. Building upon our previous work, which enabled photoactivation localization microscopy in organic solvents, herein, we present the imaging of one-dimensional supramolecular polymers in their native environment by interface point accumulation for imaging in nanoscale topography (iPAINT). The noncovalent staining, typical of iPAINT, allows the investigation of supramolecular polymers\u2019 structure in situ without any chemical modification. The quasi-permanent adsorption of the dye to the polymer is exploited to identify block-like arrangements within supramolecular fibers, which were obtained upon mixing homopolymers that were prestained with different colors. The staining of the blocks, maintained by the lack of exchange of the dyes, permits the imaging of complex structures for multiple days. This study showcases the potential of PAINT-like strategies such as iPAINT to visualize multicomponent dynamic systems in their native environment with an easy, synthesis-free approach and high spatial resolution.<\/jats:p>","DOI":"10.1021\/acsnano.8b00396","type":"journal-article","created":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T12:55:37Z","timestamp":1524747337000},"page":"4431-4439","source":"Crossref","is-referenced-by-count":44,"title":["Painting\nSupramolecular Polymers in Organic Solvents\nby Super-resolution Microscopy"],"prefix":"10.1021","volume":"12","author":[{"given":"Beatrice","family":"Adelizzi","sequence":"first","affiliation":[{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]},{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Aloi","sequence":"additional","affiliation":[{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]},{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]},{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7899-9163","authenticated-orcid":true,"given":"Nathan J.","family":"Van Zee","sequence":"additional","affiliation":[{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]},{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7201-1548","authenticated-orcid":true,"given":"Anja R. A.","family":"Palmans","sequence":"additional","affiliation":[{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]},{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4126-7492","authenticated-orcid":true,"given":"E. W.","family":"Meijer","sequence":"additional","affiliation":[{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]},{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3543-4821","authenticated-orcid":true,"given":"Ilja K.","family":"Voets","sequence":"additional","affiliation":[{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]},{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]},{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]},{"name":"Eindhoven University of Technology , , , P.O. Box 513, ,","place":["Eindhoven, The Netherlands, 5600 MB"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2018,4,26]]},"reference":[{"key":"2026090404243289700_cit1","doi-asserted-by":"publisher","first-page":"2400","DOI":"10.1126\/science.1071063","article-title":"Toward\nSelf-organization and Complex Matter","volume":"295","author":"Lehn","year":"2002","journal-title":"Science"},{"key":"2026090404243289700_cit2","doi-asserted-by":"publisher","first-page":"4759","DOI":"10.1021\/acs.macromol.5b00890","article-title":"Coding\nMacromolecules: Inputting Information in Polymers Using Monomer-Based\nAlphabets","volume":"48","author":"Lutz","year":"2015","journal-title":"Macromolecules"},{"key":"2026090404243289700_cit3","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1038\/nature11875","article-title":"Towards Practical,\nHigh-Capacity,\nLow-Maintenance Information Storage in Synthesized DNA","volume":"494","author":"Goldman","year":"2013","journal-title":"Nature"},{"key":"2026090404243289700_cit4","doi-asserted-by":"publisher","first-page":"4763","DOI":"10.1073\/pnas.072065599","article-title":"Toward\nComplex Matter: Supramolecular Chemistry and Self-Organization","volume":"99","author":"Lehn","year":"2002","journal-title":"Proc. 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