{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T03:13:59Z","timestamp":1788405239937,"version":"build-2803163510"},"reference-count":76,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2019,7,10]],"date-time":"2019-07-10T00:00:00Z","timestamp":1562716800000},"content-version":"vor","delay-in-days":7,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_ccbyncnd_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01 HL118440"],"award-info":[{"award-number":["R01 HL118440"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01 HL125703"],"award-info":[{"award-number":["R01 HL125703"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R01 CA155432"],"award-info":[{"award-number":["R01 CA155432"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["016.136.324"],"award-info":[{"award-number":["016.136.324"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["016.156.327"],"award-info":[{"award-number":["016.156.327"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["016.176.622"],"award-info":[{"award-number":["016.176.622"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["15851"],"award-info":[{"award-number":["15851"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Sensi Pharma B.V.","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,12,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Atherosclerosis is associated with a compromised endothelial barrier, facilitating the accumulation of immune cells and macromolecules in atherosclerotic lesions. In this study, we investigate endothelial barrier integrity and the enhanced permeability and retention (EPR) effect during atherosclerosis progression and therapy in Apoe\u2013\/\u2013 mice using hyaluronan nanoparticles (HA-NPs). Utilizing ultrastructural and en face plaque imaging, we uncover a significantly decreased junction continuity in the atherosclerotic plaque-covering endothelium compared to the normal vessel wall, indicative of disrupted endothelial barrier. Intriguingly, the plaque advancement had a positive effect on junction stabilization, which correlated with a 3-fold lower accumulation of in vivo administrated HA-NPs in advanced plaques compared to early counterparts. Furthermore, by using super-resolution and correlative light and electron microscopy, we trace nanoparticles in the plaque microenvironment. We find nanoparticle-enriched endothelial junctions, containing 75% of detected HA-NPs, and a high HA-NP accumulation in the endothelium-underlying extracellular matrix, which suggest an endothelial junctional traffic of HA-NPs to the plague. Finally, we probe the EPR effect by HA-NPs in the context of metabolic therapy with a glycolysis inhibitor, 3PO, proposed as a vascular normalizing strategy. The observed trend of attenuated HA-NP uptake in aortas of 3PO-treated mice coincides with the endothelial silencing activity of 3PO, demonstrated in vitro. Interestingly, the therapy also reduced the plaque inflammatory burden, while activating macrophage metabolism. Our findings shed light on natural limitations of nanoparticle accumulation in atherosclerotic plaques and provide mechanistic insight into nanoparticle trafficking across the atherosclerotic endothelium. Furthermore, our data contribute to the rising field of endothelial barrier modulation in atherosclerosis.<\/jats:p>","DOI":"10.1021\/acsnano.8b08875","type":"journal-article","created":{"date-parts":[[2019,7,3]],"date-time":"2019-07-03T16:51:51Z","timestamp":1562172711000},"page":"13759-13774","source":"Crossref","is-referenced-by-count":106,"title":["Nanoparticle-Aided\nCharacterization of Arterial Endothelial\nArchitecture during Atherosclerosis Progression and Metabolic Therapy"],"prefix":"10.1021","volume":"13","author":[{"given":"Thijs J.","family":"Beldman","sequence":"first","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsveta S.","family":"Malinova","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Desclos","sequence":"additional","affiliation":[{"name":"Academic Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita E.","family":"Grootemaat","sequence":"additional","affiliation":[{"name":"Academic Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aresh L. S.","family":"Misiak","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"van der Velden","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy P. A. A.","family":"van Roomen","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Beckers","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henk A.","family":"van Veen","sequence":"additional","affiliation":[{"name":"Academic Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Przemyslaw M.","family":"Krawczyk","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ron A.","family":"Hoebe","sequence":"additional","affiliation":[{"name":"Academic Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith C.","family":"Sluimer","sequence":"additional","affiliation":[{"name":"Maastricht University Medical Center , , ,","place":["Maastricht, The Netherlands, 6229 ER"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette E.","family":"Neele","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menno P. J.","family":"de Winther","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]},{"name":"Ludwig Maximilians University , , ,","place":["Munich, Germany, 80336"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole N.","family":"van der Wel","sequence":"additional","affiliation":[{"name":"Academic Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Lutgens","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]},{"name":"Ludwig Maximilians University , , ,","place":["Munich, Germany, 80336"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8665-3878","authenticated-orcid":true,"given":"Willem J. M.","family":"Mulder","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]},{"name":"Icahn School of Medicine at Mount Sinai , , , ,","place":["New York, New York, United States, 10029"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Huveneers","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7040-0427","authenticated-orcid":true,"given":"Ewelina","family":"Kluza","sequence":"additional","affiliation":[{"name":"Amsterdam University Medical Center , , ,","place":["Amsterdam, The Netherlands, 1105 AZ"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2019,7,3]]},"reference":[{"key":"2026090222272594600_cit1","doi-asserted-by":"publisher","first-page":"3065","DOI":"10.1038\/ncomms4065","article-title":"A Statin-Loaded Reconstituted High-Density Lipoprotein Nanoparticle\nInhibits Atherosclerotic Plaque Inflammation","volume":"5","author":"Duivenvoorden","year":"2014","journal-title":"Nat. Commun."},{"key":"2026090222272594600_cit2","doi-asserted-by":"publisher","first-page":"2020","DOI":"10.1021\/mp100309y","article-title":"Multimodal Clinical\nImaging To Longitudinally Assess a Nanomedical Anti-Inflammatory Treatment\nin Experimental Atherosclerosis","volume":"7","author":"Lobatto","year":"2010","journal-title":"Mol. Pharmaceutics"},{"key":"2026090222272594600_cit3","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1038\/nrc.2016.108","article-title":"Cancer\nNanomedicine: Progress, Challenges and Opportunities","volume":"17","author":"Shi","year":"2017","journal-title":"Nat. Rev. Cancer"},{"key":"2026090222272594600_cit4","doi-asserted-by":"publisher","first-page":"1003","DOI":"10.1016\/j.nano.2014.12.003","article-title":"Therapeutic Applications of Nanomedicine\nin Autoimmune Diseases: From Immunosuppression to Tolerance Induction","volume":"11","author":"Gharagozloo","year":"2015","journal-title":"Nanomedicine (N. Y., NY, U. S.)"},{"key":"2026090222272594600_cit5","doi-asserted-by":"publisher","first-page":"239sr1","DOI":"10.1126\/scitranslmed.3005101","article-title":"Imaging and\nNanomedicine in Inflammatory Atherosclerosis","volume":"6","author":"Mulder","year":"2014","journal-title":"Sci. Transl. Med."},{"key":"2026090222272594600_cit6","first-page":"6387","article-title":"A New Concept for Macromolecular Therapeutics in Cancer Chemotherapy:\nMechanism of Tumoritropic Accumulation of Proteins and the Antitumor\nAgent Smancs","volume":"46","author":"Matsumura","year":"1986","journal-title":"Cancer Res."},{"key":"2026090222272594600_cit7","first-page":"243","article-title":"Effective Targeting\nof Solid Tumors\nin Patients with Locally Advanced Cancers by Radiolabeled Pegylated\nLiposomes","volume":"7","author":"Harrington","year":"2001","journal-title":"Clin. Cancer Res."},{"key":"2026090222272594600_cit8","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1080\/028418600430789","article-title":"High Intratumoral Accumulation of Stealth Liposomal\nDoxorubicin in Sarcomas: Rationale for Combination with Radiotherapy","volume":"39","author":"Koukourakis","year":"2000","journal-title":"Acta Oncol"},{"key":"2026090222272594600_cit9","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.jconrel.2016.11.015","article-title":"To Exploit\nthe Tumor Microenvironment: Since the EPR Effect Fails in the Clinic,\nWhat Is the Future of Nanomedicine?","volume":"244","author":"Danhier","year":"2016","journal-title":"J. Controlled\nRelease"},{"key":"2026090222272594600_cit10","doi-asserted-by":"publisher","first-page":"2412","DOI":"10.1158\/0008-5472.CAN-12-4561","article-title":"Challenges and Key Considerations\nof the Enhanced Permeability and Retention Effect for Nanomedicine\nDrug Delivery in Oncology","volume":"73","author":"Prabhakar","year":"2013","journal-title":"Cancer Res."},{"key":"2026090222272594600_cit11","doi-asserted-by":"publisher","first-page":"4190","DOI":"10.1158\/1078-0432.CCR-16-3193","article-title":"64 Cu-MM-302 Positron\nEmission Tomography Quantifies Variability of\nEnhanced Permeability and Retention of Nanoparticles in Relation to\nTreatment Response in Patients with Metastatic Breast Cancer","volume":"23","author":"Lee","year":"2017","journal-title":"Clin. Cancer Res."},{"key":"2026090222272594600_cit12","doi-asserted-by":"publisher","first-page":"1039","DOI":"10.1016\/j.nano.2015.02.021","article-title":"Prednisolone-Containing Liposomes\nAccumulate in Human Atherosclerotic Macrophages upon Intravenous Administration","volume":"11","author":"van der Valk","year":"2015","journal-title":"Nanomedicine (N. Y., NY, U. S.)"},{"key":"2026090222272594600_cit13","doi-asserted-by":"publisher","first-page":"8003","DOI":"10.1039\/C5NR01050K","article-title":"Silica-Gold Nanoparticles\nfor Atheroprotective Management\nof Plaques: Results of the NANOM-FIM Trial","volume":"7","author":"Kharlamov","year":"2015","journal-title":"Nanoscale"},{"key":"2026090222272594600_cit14","doi-asserted-by":"publisher","first-page":"1135","DOI":"10.1161\/hc0902.104353","article-title":"Inflammation\nand Atherosclerosis","volume":"105","author":"Libby","year":"2002","journal-title":"Circulation"},{"key":"2026090222272594600_cit15","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1161\/CIRCRESAHA.115.306301","article-title":"Endothelial\nCell Dysfunction and\nthe Pathobiology of Atherosclerosis","volume":"118","author":"Gimbrone","year":"2016","journal-title":"Circ. Res."},{"key":"2026090222272594600_cit16","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1002\/ccd.21088","article-title":"Heterogeneity\nof Atherosclerotic Plaque Characteristics in Human Coronary Artery\nDisease: A Three-Dimensional Intravascular Ultrasound Study. Catheter","volume":"70","author":"Box","year":"2007","journal-title":"Cardiovasc. Interv."},{"key":"2026090222272594600_cit17","doi-asserted-by":"publisher","first-page":"S3","DOI":"10.1016\/j.amjmed.2008.10.013","article-title":"The Pathology\nof Atherosclerosis: Plaque Development and Plaque Responses to Medical\nTreatment","volume":"122","author":"Insull","year":"2009","journal-title":"Am. J. Med."},{"key":"2026090222272594600_cit18","doi-asserted-by":"publisher","first-page":"5785","DOI":"10.1021\/acsnano.7b01385","article-title":"Hyaluronan Nanoparticles\nSelectively Target Plaque-Associated Macrophages and Improve Plaque\nStability in Atherosclerosis","volume":"11","author":"Beldman","year":"2017","journal-title":"ACS Nano"},{"key":"2026090222272594600_cit19","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1016\/j.ccell.2016.10.006","article-title":"Inhibition\nof the Glycolytic Activator PFKFB3 in Endothelium Induces Tumor Vessel\nNormalization, Impairs Metastasis, and Improves Chemotherapy","volume":"30","author":"Cantelmo","year":"2016","journal-title":"Cancer Cell"},{"key":"2026090222272594600_cit20","doi-asserted-by":"publisher","first-page":"1078","DOI":"10.1073\/pnas.1322725111","article-title":"Probing Nanoparticle\nTranslocation across the Permeable Endothelium in Experimental Atherosclerosis","volume":"111","author":"Kim","year":"2014","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026090222272594600_cit21","doi-asserted-by":"publisher","first-page":"1837","DOI":"10.1021\/nn506750r","article-title":"Atherosclerotic\nPlaque Targeting\nMechanism of Long-Circulating Nanoparticles Established by Multimodal\nImaging","volume":"9","author":"Lobatto","year":"2015","journal-title":"ACS Nano"},{"key":"2026090222272594600_cit22","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCIMAGING.116.005813","article-title":"Atheroma Susceptible to Thrombosis Exhibit Impaired Endothelial Permeability In Vivo as Assessed by Nanoparticle-Based Fluorescence Molecular\nImaging. Circ","volume":"10","author":"Stein-Merlob","year":"2017","journal-title":"Cardiovascular\nImaging"},{"key":"2026090222272594600_cit23","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1002\/iub.485","article-title":"Delivery\nof Nanoparticle-Complexed Drugs across the Vascular Endothelial Barrier via Caveolae","volume":"63","author":"Wang","year":"2011","journal-title":"IUBMB Life"},{"key":"2026090222272594600_cit24","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/j.nantod.2014.04.008","article-title":"Challenges\nAssociated with Penetration of Nanoparticles\nacross Cell and Tissue Barriers: A Review of Current Status and Future\nProspects","volume":"9","author":"Barua","year":"2014","journal-title":"Nano Today"},{"key":"2026090222272594600_cit25","doi-asserted-by":"publisher","first-page":"3437","DOI":"10.1007\/s12274-016-1220-9","article-title":"Uptake of Citrate-Coated Iron Oxide Nanoparticles into\nAtherosclerotic Lesions in Mice Occurs via Accelerated\nTranscytosis through Plaque Endothelial Cells","volume":"9","author":"Poller","year":"2016","journal-title":"Nano Res."},{"key":"2026090222272594600_cit26","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1161\/01.ATV.14.1.133","article-title":"ApoE-Deficient Mice Develop Lesions of All Phases of Atherosclerosis\nthroughout the Arterial Tree","volume":"14","author":"Nakashima","year":"1994","journal-title":"Arterioscler.\nThromb."},{"key":"2026090222272594600_cit27","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1161\/ATVBAHA.107.158014","article-title":"VE-Cadherin:\nThe Major Endothelial Adhesion Molecule Controlling Cellular Junctions\nand Blood Vessel Formation","volume":"28","author":"Vestweber","year":"2008","journal-title":"Arterioscler., Thromb.,\nVasc. Biol."},{"key":"2026090222272594600_cit28","doi-asserted-by":"publisher","first-page":"2115","DOI":"10.1242\/jcs.017897","article-title":"The Role of Adherens Junctions and\nVE-Cadherin in the Control of Vascular Permeability","volume":"121","author":"Dejana","year":"2008","journal-title":"J. Cell Sci."},{"key":"2026090222272594600_cit29","doi-asserted-by":"publisher","first-page":"2059","DOI":"10.1161\/ATVBAHA.114.304180","article-title":"F-Actin\u2013Anchored Focal Adhesions\nDistinguish Endothelial Phenotypes of Human Arteries and Veins","volume":"34","author":"van Geemen","year":"2014","journal-title":"Arterioscler., Thromb., Vasc. Biol."},{"key":"2026090222272594600_cit30","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1016\/j.tcb.2017.11.002","article-title":"Sensing of\nCytoskeletal Forces by Asymmetric Adherens\nJunctions","volume":"28","author":"Malinova","year":"2018","journal-title":"Trends Cell Biol."},{"key":"2026090222272594600_cit31","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.113.000241","article-title":"Hypercholesterolemia\nInduces Oxidant Stress That Accelerates the Ageing of Hematopoietic\nStem Cells","volume":"3","author":"Tie","year":"2014","journal-title":"J. Am. Heart Assoc."},{"key":"2026090222272594600_cit32","first-page":"99","article-title":"Endothelial Permeability in Atherosclerosis","volume":"76","author":"Friedman","year":"1963","journal-title":"Arch. Pathol."},{"key":"2026090222272594600_cit33","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1161\/CIRCULATIONAHA.112.092098","article-title":"Noninvasive\nMagnetic Resonance Imaging Evaluation of Endothelial Permeability\nin Murine Atherosclerosis Using an Albumin-Binding Contrast Agent","volume":"126","author":"Phinikaridou","year":"2012","journal-title":"Circulation"},{"key":"2026090222272594600_cit34","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1160\/th15-09-0765","article-title":"Endothelial\nErosion of Plaques as\na Substrate for Coronary Thrombosis","volume":"115","author":"White","year":"2016","journal-title":"Thromb.\nHaemostasis"},{"key":"2026090222272594600_cit35","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1097\/MOL.0000000000000440","article-title":"Mechanisms\nof Erosion of Atherosclerotic Plaques","volume":"28","author":"Quillard","year":"2017","journal-title":"Curr.\nOpin. Lipidol."},{"key":"2026090222272594600_cit36","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1093\/cvr\/cvn099","article-title":"Early Atherosclerosis\nin Humans:\nRole of Diffuse Intimal Thickening and Extracellular Matrix Proteoglycans","volume":"79","author":"Nakashima","year":"2008","journal-title":"Cardiovasc. Res."},{"key":"2026090222272594600_cit37","doi-asserted-by":"publisher","first-page":"16014","DOI":"10.1038\/natrevmats.2016.14","article-title":"Analysis of Nanoparticle Delivery\nto Tumours","volume":"1","author":"Wilhelm","year":"2016","journal-title":"Nat. Rev. Mater."},{"key":"2026090222272594600_cit38","doi-asserted-by":"publisher","first-page":"1726","DOI":"10.1161\/01.CIR.99.13.1726","article-title":"Angiogenesis Inhibitors\nEndostatin or TNP-470 Reduce\nIntimal Neovascularization and Plaque Growth in Apolipoprotein E-Deficient\nMice","volume":"99","author":"Moulton","year":"1999","journal-title":"Circulation"},{"key":"2026090222272594600_cit39","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1016\/0046-8177(95)90148-5","article-title":"Intimal Neovascularization in Human\nCoronary Atherosclerosis: Its Origin and Pathophysiological Significance","volume":"26","author":"Kumamoto","year":"1995","journal-title":"Hum. Pathol."},{"key":"2026090222272594600_cit40","doi-asserted-by":"publisher","first-page":"2032","DOI":"10.1161\/01.CIR.0000143233.87854.23","article-title":"Plaque\nNeovascularization Is Increased in Ruptured Atherosclerotic Lesions\nof Human Aorta","volume":"110","author":"Moreno","year":"2004","journal-title":"Circulation"},{"key":"2026090222272594600_cit41","doi-asserted-by":"publisher","first-page":"2129","DOI":"10.1161\/CIRCULATIONAHA.111.030627","article-title":"Intravital\nMicroscopy on Atherosclerosis in Apolipoprotein E\u2013Deficient\nMice Establishes Microvessels as Major Entry Pathways for Leukocytes\nto Advanced Lesions","volume":"124","author":"Eriksson","year":"2011","journal-title":"Circulation"},{"key":"2026090222272594600_cit42","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1161\/01.ATV.0000196565.38679.6d","article-title":"Correlation of Vasa\nVasorum Neovascularization and Plaque Progression in Aortas of Apolipoprotein\nE \u2013\/\u2013 \/Low-Density Lipoprotein \u2013\/\u2013 Double Knockout Mice","volume":"26","author":"Langheinrich","year":"2006","journal-title":"Arterioscler., Thromb.,\nVasc. Biol."},{"key":"2026090222272594600_cit43","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1161\/ATVBAHA.112.300087","article-title":"Plaque-Associated\nVasa Vasorum in Aged Apolipoprotein E\u2013Deficient Mice Exhibit\nProatherogenic Functional Features In Vivo","volume":"33","author":"Rademakers","year":"2013","journal-title":"Arterioscler., Thromb., Vasc. Biol."},{"key":"2026090222272594600_cit44","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1172\/JCI29950","article-title":"Ly-6Chi Monocytes Dominate Hypercholesterolemia-Associated\nMonocytosis and Give Rise to Macrophages in Atheromata","volume":"117","author":"Swirski","year":"2007","journal-title":"J. Clin. Invest."},{"key":"2026090222272594600_cit45","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1038\/nri3908","article-title":"How Leukocytes\nCross the Vascular Endothelium","volume":"15","author":"Vestweber","year":"2015","journal-title":"Nat. Rev. Immunol."},{"key":"2026090222272594600_cit46","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1161\/CIRCULATIONAHA.107.741181","article-title":"Nanoparticle\nPET-CT Imaging of Macrophages in Inflammatory Atherosclerosis","volume":"117","author":"Nahrendorf","year":"2008","journal-title":"Circulation"},{"key":"2026090222272594600_cit47","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1021\/bc500517k","article-title":"HDL-Mimetic PLGA Nanoparticle To Target Atherosclerosis\nPlaque Macrophages","volume":"26","author":"Sanchez-Gaytan","year":"2015","journal-title":"Bioconjugate Chem."},{"key":"2026090222272594600_cit48","doi-asserted-by":"publisher","first-page":"E6731","DOI":"10.1073\/pnas.1609629113","article-title":"Immune Cell Screening\nof a Nanoparticle Library Improves Atherosclerosis Therapy","volume":"113","author":"Tang","year":"2016","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026090222272594600_cit49","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1161\/01.CIR.84.2.755","article-title":"Hypercholesterolemia Enhances Macrophage\nRecruitment and Dysfunction of Regenerated Endothelium after Balloon\nInjury of the Rabbit Iliac Artery","volume":"84","author":"Weidinger","year":"1991","journal-title":"Circulation"},{"key":"2026090222272594600_cit50","doi-asserted-by":"publisher","first-page":"1517","DOI":"10.1016\/j.jacc.2008.12.056","article-title":"Thin-Walled\nMicrovessels in Human\nCoronary Atherosclerotic Plaques Show Incomplete Endothelial Junctions\nRelevance of Compromised Structural Integrity for Intraplaque Microvascular\nLeakage","volume":"53","author":"Sluimer","year":"2009","journal-title":"J. Am. Coll. Cardiol."},{"key":"2026090222272594600_cit51","first-page":"713","article-title":"Ultrastructural\nAlterations of Endothelium Covering Advanced Atherosclerotic Plaque\nin Human Carotid Artery Visualised by Scanning Electron Microscope","volume":"53","author":"Walski","year":"2002","journal-title":"J. Physiol. Pharmacol."},{"key":"2026090222272594600_cit52","doi-asserted-by":"crossref","first-page":"53","DOI":"10.36253\/ijae-1058","article-title":"Atherosclerotic Alterations in Human Carotid Observed\nby Scanning Electron Microscopy","volume":"115","author":"Carlo","year":"2010","journal-title":"Ital. J. Anat.\nEmbryol."},{"key":"2026090222272594600_cit53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2013\/916254","article-title":"Targeted\nDrug Delivery to Endothelial Adhesion Molecules","volume":"2013","author":"Muzykantov","year":"2013","journal-title":"ISRN Vasc. Med."},{"key":"2026090222272594600_cit54","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1042\/bj2340653","article-title":"Binding of\nHyaluronate and Chondroitin Sulphate to Liver Endothelial Cells","volume":"234","author":"Laurent","year":"1986","journal-title":"Biochem. J."},{"key":"2026090222272594600_cit55","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1080\/10717544.2018.1450910","article-title":"Application of Hyaluronic Acid as Carriers in Drug Delivery","volume":"25","author":"Huang","year":"2018","journal-title":"Drug Delivery"},{"key":"2026090222272594600_cit56","doi-asserted-by":"publisher","first-page":"1599","DOI":"10.1242\/jcs.00367","article-title":"A Novel Endocytic Pathway\nInduced\nby Clustering Endothelial ICAM-1 or PECAM-1","volume":"116","author":"Muro","year":"2003","journal-title":"J. Cell Sci."},{"key":"2026090222272594600_cit57","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.ymthe.2005.07.687","article-title":"Lysosomal Enzyme Delivery by ICAM-1-Targeted\nNanocarriers Bypassing Glycosylation- and Clathrin-Dependent Endocytosis","volume":"13","author":"Muro","year":"2006","journal-title":"Mol. Ther."},{"key":"2026090222272594600_cit58","doi-asserted-by":"publisher","first-page":"1246","DOI":"10.1124\/jpet.105.097139","article-title":"Covalent Linkage\nof\nApolipoprotein E to Albumin Nanoparticles Strongly Enhances Drug Transport\ninto the Brain","volume":"317","author":"Michaelis","year":"2006","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"2026090222272594600_cit59","doi-asserted-by":"publisher","first-page":"950","DOI":"10.1016\/j.jcmg.2016.01.020","article-title":"In Vivo PET Imaging of HDL in Multiple Atherosclerosis Models","volume":"9","author":"Pe\u0301rez-Medina","year":"2016","journal-title":"JACC Cardiovasc. Imaging"},{"key":"2026090222272594600_cit60","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1016\/j.immuni.2018.09.008","article-title":"Inhibiting\nInflammation with Myeloid Cell-Specific\nNanobiologics Promotes Organ Transplant Acceptance","volume":"49","author":"Braza","year":"2018","journal-title":"Immunity"},{"key":"2026090222272594600_cit61","doi-asserted-by":"publisher","first-page":"1707","DOI":"10.1007\/s002590100626","article-title":"FDG-PET for Clinical Use","volume":"28","author":"Reske","year":"2001","journal-title":"Eur.\nJ. Nucl. Med."},{"key":"2026090222272594600_cit62","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1006\/exmp.2001.2368","article-title":"TNF Signaling in Vascular Endothelial Cells","volume":"70","author":"Madge","year":"2001","journal-title":"Exp. Mol. Pathol."},{"key":"2026090222272594600_cit63","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1083\/jcb.201108120","article-title":"Vinculin Associates\nwith Endothelial VE-Cadherin Junctions to Control Force-Dependent\nRemodeling","volume":"196","author":"Huveneers","year":"2012","journal-title":"J. Cell Biol."},{"key":"2026090222272594600_cit64","doi-asserted-by":"publisher","first-page":"L1232","DOI":"10.1152\/ajplung.00109.2006","article-title":"TNF-\u03b1\nIncreases Tyrosine Phosphorylation of Vascular Endothelial Cadherin\nand Opens the Paracellular Pathway through Fyn Activation in Human\nLung Endothelia","volume":"291","author":"Angelini","year":"2006","journal-title":"Am. J. Physiol."},{"key":"2026090222272594600_cit65","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1002\/(SICI)1097-4652(199807)176:1&lt;150::AID-JCP17&gt;3.0.CO;2-B","article-title":"Regulation of TNF-\u03b1-Induced\nReorganization of the Actin Cytoskeleton and Cell-Cell Junctions by\nRho, Rac, and Cdc42 in Human Endothelial Cells","volume":"176","author":"Wo\u0301jciak-Stothard","year":"1998","journal-title":"J. Cell. Physiol."},{"key":"2026090222272594600_cit66","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1080\/14728222.2017.1282465","article-title":"Vessel Pruning\nor Healing: Endothelial Metabolism as a Novel Target?","volume":"21","author":"Cantelmo","year":"2017","journal-title":"Expert Opin. Ther. Targets"},{"key":"2026090222272594600_cit67","doi-asserted-by":"publisher","first-page":"2708","DOI":"10.1161\/01.CIR.0000020548.60110.76","article-title":"Imaging\nAtherosclerotic Plaque Inflammation With [18F]-Fluorodeoxyglucose\nPositron Emission Tomography","volume":"105","author":"Rudd","year":"2002","journal-title":"Circulation"},{"key":"2026090222272594600_cit68","doi-asserted-by":"publisher","first-page":"1257","DOI":"10.1038\/nm1102-1257","article-title":"Stabilization of Atherosclerotic\nPlaques: New Mechanisms\nand Clinical Targets","volume":"8","author":"Libby","year":"2002","journal-title":"Nat. Med. (N. Y., NY,\nU. S.)"},{"key":"2026090222272594600_cit69","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1161\/CIRCRESAHA.116.302855","article-title":"Endothelial\nCell Metabolism in Normal and Diseased Vasculature","volume":"116","author":"Eelen","year":"2015","journal-title":"Circ. Res."},{"key":"2026090222272594600_cit70","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.cmet.2013.11.008","article-title":"Partial and Transient\nReduction of Glycolysis by PFKFB3 Blockade Reduces Pathological Angiogenesis","volume":"19","author":"Schoors","year":"2014","journal-title":"Cell Metab."},{"key":"2026090222272594600_cit71","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1161\/ATVBAHA.115.305551","article-title":"HIF-1\u03b1 and PFKFB3Mediate a Tight Relationship\nBetween Proinflammatory Activation and Anerobic Metabolism in Atherosclerotic\nMacrophages","volume":"35","author":"Tawakol","year":"2015","journal-title":"Arterioscler., Thromb., Vasc. Biol."},{"key":"2026090222272594600_cit72","doi-asserted-by":"publisher","first-page":"871","DOI":"10.2967\/jnumed.107.050294","article-title":"Atherosclerosis\nInflammation Imaging with 18F-FDG PET: Carotid, Iliac, and Femoral\nUptake Reproducibility, Quantification Methods, and Recommendations","volume":"49","author":"Rudd","year":"2008","journal-title":"J. Nucl. Med."},{"key":"2026090222272594600_cit73","doi-asserted-by":"publisher","first-page":"2785","DOI":"10.1158\/1078-0432.CCR-04-2626","article-title":"Progress and Promise\nof FDG-PET Imaging for Cancer Patient Management and Oncologic Drug\nDevelopment","volume":"11","author":"Kelloff","year":"2005","journal-title":"Clin. Cancer Res."},{"key":"2026090222272594600_cit74","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1016\/j.jacc.2011.03.044","article-title":"Hypoxia but Not Inflammation\nAugments Glucose Uptake in Human Macrophages: Implications for Imaging\nAtherosclerosis with 18fluorine-Labeled 2-Deoxy-D-Glucose Positron\nEmission Tomography","volume":"58","author":"Folco","year":"2011","journal-title":"J. Am. Coll. Cardiol."},{"key":"2026090222272594600_cit75","doi-asserted-by":"publisher","first-page":"2389","DOI":"10.1093\/bioinformatics\/btu202","article-title":"ThunderSTORM: A\nComprehensive ImageJ Plug-in for PALM\nand STORM Data Analysis and Super-Resolution Imaging","volume":"30","author":"Ovesny\u0301","year":"2014","journal-title":"Bioinformatics"},{"key":"2026090222272594600_cit76","doi-asserted-by":"publisher","first-page":"1374","DOI":"10.1177\/0271678X16655550","article-title":"Paravascular Channels,\nCisterns,\nand the Subarachnoid Space in the Rat Brain: A Single Compartment\nwith Preferential Pathways","volume":"37","author":"Bedussi","year":"2017","journal-title":"J. Cereb. Blood\nFlow Metab."}],"container-title":["ACS Nano"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsnano.8b08875","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/13\/12\/13759\/67098581\/acsnano.8b08875.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/ancac3\/article-pdf\/13\/12\/13759\/67098581\/acsnano.8b08875.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:27:55Z","timestamp":1788402475000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/ancac3\/article\/13\/12\/13759\/801814\/Nanoparticle-Aided-Characterization-of-Arterial"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,3]]},"references-count":76,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2019,7,3]]},"published-print":{"date-parts":[[2019,12,24]]}},"URL":"https:\/\/doi.org\/10.1021\/acsnano.8b08875","relation":{},"ISSN":["1936-0851","1936-086X"],"issn-type":[{"value":"1936-0851","type":"print"},{"value":"1936-086X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,3]]}}}