{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T20:41:59Z","timestamp":1792356119289,"version":"4.4.0"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"15","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_ccbyncnd_termsofuse.html"}],"funder":[{"DOI":"10.13039\/501100004578","name":"Ministerstvo Prumyslu a Obchodu","doi-asserted-by":"publisher","award":["FV10416"],"award-info":[{"award-number":["FV10416"]}],"id":[{"id":"10.13039\/501100004578","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,21]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Electrospun materials made from biodegradable polycaprolactone are used widely in various tissue engineering and regenerative medicine applications because of their morphological similarity to the extracellular matrix. However, the main prerequisite for the use of such materials in clinical practice consists of the selection of the appropriate sterilization technique. This study is devoted to the study of the impact of traditional sterilization and disinfection methods on a nanofibrous polycaprolactone layer constructed by means of the needleless electrospinning technique. It was determined that hydrogen peroxide plasma treatment led to the loss of fibrous morphology and the creation of a foil. However, certain sterilization (ethylene oxide, gamma irradiation, and peracetic acid) and disinfection techniques (ethanol and UV irradiation) were found not to lead to a change in morphology; thus, the study investigates their impact on thermal properties, molecular weight, and interactions with a fibroblast cell line. It was determined that the surface properties that guide cell adhesion and proliferation were affected more than the bulk properties. The highest proliferation rate of fibroblasts seeded on nanofibrous scaffolds was observed with respect to gamma-irradiated polycaprolactone, while the lowest proliferation rate was observed following ethylene oxide sterilization.<\/jats:p>","DOI":"10.1021\/acsomega.0c00503","type":"journal-article","created":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T07:43:08Z","timestamp":1586245388000},"page":"8885-8892","source":"Crossref","is-referenced-by-count":64,"title":["Impact of Various Sterilization and\nDisinfection Techniques on Electrospun Poly-\u03b5-caprolactone"],"prefix":"10.1021","volume":"5","author":[{"given":"Jana","family":"Horakova","sequence":"first","affiliation":[{"name":"Technical University of Liberec , , Studentska 1402\/2 , ,","place":["Liberec, Czech Republic, 460 01"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marketa","family":"Klicova","sequence":"additional","affiliation":[{"name":"Technical University of Liberec , , Studentska 1402\/2 , ,","place":["Liberec, Czech Republic, 460 01"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9856-1604","authenticated-orcid":true,"given":"Jakub","family":"Erben","sequence":"additional","affiliation":[{"name":"Technical University of Liberec , , Studentska 1402\/2 , ,","place":["Liberec, Czech Republic, 460 01"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Klapstova","sequence":"additional","affiliation":[{"name":"Technical University of Liberec , , Studentska 1402\/2 , ,","place":["Liberec, Czech Republic, 460 01"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vit","family":"Novotny","sequence":"additional","affiliation":[{"name":"Technical University of Liberec , , Studentska 1402\/2 , ,","place":["Liberec, Czech Republic, 460 01"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubos","family":"Behalek","sequence":"additional","affiliation":[{"name":"Technical University of Liberec , , Studentska 1402\/2 , ,","place":["Liberec, Czech Republic, 460 01"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Chvojka","sequence":"additional","affiliation":[{"name":"Technical University of Liberec , , Studentska 1402\/2 , ,","place":["Liberec, Czech Republic, 460 01"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"2026092109075683600_cit1","first-page":"163","volume-title":"Guideline\nfor Disinfection and Sterilization in Healthcare Facilities","author":"Rutala","year":"2008"},{"key":"2026092109075683600_cit2","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.eurpolymj.2016.07.016","article-title":"Impact of Sterilization Methods on Electrospun Scaffolds\nfor Tissue Engineering","volume":"82","author":"Rediguieri","year":"2016","journal-title":"Eur. Polym. J."},{"key":"2026092109075683600_cit3","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.actbio.2013.08.035","article-title":"Effect of\nSterilization on Structural and Material Properties of 3-D Silk Fibroin\nScaffolds","volume":"10","author":"Hofmann","year":"2014","journal-title":"Acta Biomater."},{"key":"2026092109075683600_cit4","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1002\/mabi.201500013","article-title":"The Effect of Sterilization\non Silk Fibroin Biomaterial Properties","volume":"15","author":"Rnjak-Kovacina","year":"2015","journal-title":"Macromol.\nBiosci."},{"key":"2026092109075683600_cit5","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.1002\/jbm.820291209","article-title":"Polyvinylidene\nFluoride (PVDF) as a Biomaterial: From Polymeric Raw Material to Monofilament\nVascular Suture","volume":"29","author":"Laroche","year":"1995","journal-title":"J. Biomed. Mater. Res."},{"key":"2026092109075683600_cit6","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1080\/00914037.2014.977900","article-title":"Dose-Dependent\nEffects of Gamma Irradiation on the Materials Properties and Cell\nProliferation of Electrospun Polycaprolactone Tissue Engineering Scaffolds","volume":"64","author":"Augustine","year":"2015","journal-title":"Int. J. Polym. Mater. Polym. Biomater."},{"key":"2026092109075683600_cit7","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1080\/714975831","article-title":"Effects of Gamma\nRadiation on a Plastic Material Based on Bean Protein","volume":"51","author":"Gonzalez","year":"2002","journal-title":"Int. J. Polym. Mater."},{"key":"2026092109075683600_cit8","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1089\/ten.tec.2013.0624","article-title":"Peracetic\nAcid: A Practical Agent for Sterilizing Heat-Labile Polymeric Tissue-Engineering\nScaffolds","volume":"20","author":"Yoganarasimha","year":"2014","journal-title":"Tissue Eng., Part C"},{"key":"2026092109075683600_cit9","doi-asserted-by":"publisher","first-page":"12351","DOI":"10.1039\/c5nr02922h","article-title":"A Battery-Operated\nPortable Handheld Electrospinning Apparatus","volume":"7","author":"Xu","year":"2015","journal-title":"Nanoscale"},{"key":"2026092109075683600_cit10","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1111\/iwj.12929","article-title":"Cellular Interactions\nwith Bacterial Cellulose: Polycaprolactone Nanofibrous Scaffolds Produced\nby a Portable Electrohydrodynamic Gun for Point-of-Need Wound Dressing","volume":"15","author":"Aydogdu","year":"2018","journal-title":"Int. Wound J."},{"key":"2026092109075683600_cit11","doi-asserted-by":"publisher","first-page":"19166","DOI":"10.1039\/c9nr02802a","article-title":"Advances in Portable Electrospinning\nDevices for in Situ Delivery of Personalized Wound\nCare","volume":"11","author":"Yan","year":"2019","journal-title":"Nanoscale"},{"key":"2026092109075683600_cit12","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1007\/s13204-015-0439-1","article-title":"Electrospun PCL Membranes Incorporated\nwith Biosynthesized Silver Nanoparticles as Antibacterial Wound Dressings","volume":"6","author":"Augustine","year":"2016","journal-title":"Appl. Nanosci."},{"key":"2026092109075683600_cit13","doi-asserted-by":"publisher","first-page":"730","DOI":"10.1111\/iwj.13090","article-title":"Anti-fungal\nbandages containing cinnamon extract","volume":"16","author":"Ahmed","year":"2019","journal-title":"Int. Wound\nJ."},{"key":"2026092109075683600_cit14","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/j.mtcomm.2019.02.009","article-title":"Recent Advances in\nElectrospun Polycaprolactone Based Scaffolds for Wound Healing and\nSkin Bioengineering Applications","volume":"19","author":"Joseph","year":"2019","journal-title":"Mater. Today\nCommun."},{"key":"2026092109075683600_cit15","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1177\/1528083714540701","article-title":"Design of Polycaprolactone Vascular\nGrafts","volume":"45","author":"Yalcin","year":"2016","journal-title":"J. Ind. Text."},{"key":"2026092109075683600_cit16","doi-asserted-by":"publisher","first-page":"3228","DOI":"10.1021\/bm100476r","article-title":"Cartilage Tissue\nEngineering Using Electrospun PCL Nanofiber Meshes and MSCs","volume":"11","author":"Alves da Silva","year":"2010","journal-title":"Biomacromolecules"},{"key":"2026092109075683600_cit17","doi-asserted-by":"publisher","first-page":"1134","DOI":"10.1038\/s41598-020-58113-4","article-title":"Experimental\nFortification of Intestinal Anastomoses with Nanofibrous Materials\nin a Large Animal Model","volume":"10","author":"Rosendorf","year":"2020","journal-title":"Sci. Rep."},{"key":"2026092109075683600_cit18","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1016\/j.progpolymsci.2010.04.002","article-title":"The return of a forgotten polymer-Polycaprolactone\nin the 21st century","volume":"35","author":"Woodruff","year":"2010","journal-title":"Prog. Polym. Sci."},{"key":"2026092109075683600_cit19","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.msec.2018.06.041","article-title":"The Effect of Ethylene\nOxide Sterilization on Electrospun Vascular Grafts Made from Biodegradable\nPolyesters","volume":"92","author":"Horakova","year":"2018","journal-title":"Mater. Sci. Eng. C"},{"key":"2026092109075683600_cit20","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1002\/jbm.a.35911","article-title":"Cell response to sterilized electrospun\npoly(\u03b5-caprolactone) scaffolds to aid tendon regenerationin\nvivo","volume":"105","author":"Bhaskar","year":"2017","journal-title":"J. Biomed. Mater. Res., Part A"},{"key":"2026092109075683600_cit21","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1002\/polb.23072","article-title":"Gamma Irradiation of Electrospun\nPoly(\u03b5-Caprolactone) Fibers Affects Material Properties but\nNot Cell Response","volume":"50","author":"Bosworth","year":"2012","journal-title":"J. Polym. Sci., Part B: Polym.\nPhys."},{"key":"2026092109075683600_cit22","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1177\/0883911515598795","article-title":"Optimization of sterilization\nmethods for electrospun poly(\u03b5-caprolactone) to enhance pre-osteoblast\ncell behaviors for guided bone regeneration","volume":"31","author":"Dai","year":"2016","journal-title":"J. Bioact. Compat. Polym."},{"key":"2026092109075683600_cit23","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1016\/0014-3057(72)90109-7","article-title":"Thermodynamics of Fusion of Poly-\u03b2-Propiolactone and Poly-\u03f5-Caprolactone.\nComparative Analysis of the Melting of Aliphatic Polylactone and Polyester\nChains","volume":"8","author":"Crescenzi","year":"1972","journal-title":"Eur. Polym. J."},{"key":"2026092109075683600_cit24","first-page":"234","article-title":"Nanovla\u0301kna v Hojeni\u0301\nKoz\u030cni\u0301ch Ran","volume":"4","author":"Krchova","year":"2014","journal-title":"C\u030ces. Dermatovenerol.\nC\u030casopis C\u030ces. Akad. Dermatovenerol."},{"key":"2026092109075683600_cit25","doi-asserted-by":"publisher","first-page":"025012","DOI":"10.1088\/2057-1976\/ab756f","article-title":"The Assessment\nof Electrospun Scaffolds Fabricated from Polycaprolactone with the\nAddition of L-Arginine","volume":"6","author":"Horakova","year":"2020","journal-title":"Biomed. Phys. Eng. Express"},{"key":"2026092109075683600_cit26","doi-asserted-by":"publisher","first-page":"1864","DOI":"10.1166\/jbn.2016.2308","article-title":"Designing of Combined Nano and Microfiber\nNetwork by Immobilization of Oxidized Cellulose Nanofiber on Polycaprolactone\nFibrous Scaffold","volume":"12","author":"Amirian","year":"2016","journal-title":"J. Biomed. Nanotechnol."},{"key":"2026092109075683600_cit27","doi-asserted-by":"publisher","first-page":"2466","DOI":"10.1016\/j.matlet.2009.08.037","article-title":"Fabrication of a Novel Micro\u2013nano\nFibrous Nonwoven Scaffold with Antheraea Assama Silk Fibroin for Use\nin Tissue Engineering","volume":"63","author":"Kasoju","year":"2009","journal-title":"Mater. Lett."},{"key":"2026092109075683600_cit28","doi-asserted-by":"publisher","first-page":"041401","DOI":"10.1063\/1.5110965","article-title":"Experimental\nand Theoretical Investigation of the Fluid Behavior during Polymeric\nFiber Formation with and without Pressure","volume":"6","author":"Alenezi","year":"2019","journal-title":"Appl.\nPhys. Rev."},{"key":"2026092109075683600_cit29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2017\/8952390","article-title":"Crystallinity of Electrospun and\nCentrifugal Spun Polycaprolactone Fibers: A Comparative Study","volume":"2017","author":"Kuzelova Kostakova","year":"2017","journal-title":"J. Nanomater."},{"key":"2026092109075683600_cit30","doi-asserted-by":"publisher","first-page":"43232","DOI":"10.1002\/app.43232","article-title":"Rapid Fabrication\nof Poly(\u03b5-Caprolactone) Nanofibers Using Needleless Alternating\nCurrent Electrospinning","volume":"133","author":"Lawson","year":"2016","journal-title":"J. Appl. Polym. Sci."},{"key":"2026092109075683600_cit31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1163\/092050611x612296","article-title":"Effects of Low-Temperature Hydrogen Peroxide Gas Plasma\nSterilization on In Vitro Cytotoxicity of Poly(\u03f5-Caprolactone)\n(PCL)","volume":"23","author":"Franklin","year":"2012","journal-title":"J. Biomater. Sci., Polym. Ed."},{"key":"2026092109075683600_cit32","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1016\/j.biotechadv.2011.06.004","article-title":"Modulation\nof Cell Adhesion, Proliferation and Differentiation on Materials Designed\nfor Body Implants","volume":"29","author":"Bacakova","year":"2011","journal-title":"Biotechnol. Adv."},{"key":"2026092109075683600_cit33","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.medengphy.2008.07.005","article-title":"Effect of sterilisation by gamma irradiation on the\nability of polycaprolactone (PCL) to act as a scaffold material","volume":"31","author":"Cottam","year":"2009","journal-title":"Med. Eng. Phys."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.0c00503","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/5\/15\/8885\/67719162\/acsomega.0c00503.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/5\/15\/8885\/67719162\/acsomega.0c00503.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:09:43Z","timestamp":1789996183000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/5\/15\/8885\/619446\/Impact-of-Various-Sterilization-and-Disinfection"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,7]]},"references-count":33,"journal-issue":{"issue":"15","published-online":{"date-parts":[[2020,4,7]]},"published-print":{"date-parts":[[2020,4,21]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.0c00503","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,7]]}}}