{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:20:23Z","timestamp":1788348023509,"version":"build-2803163510"},"reference-count":41,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T00:00:00Z","timestamp":1596412800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671596"],"award-info":[{"award-number":["81671596"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010877","name":"Shenzhen Science and Technology Innovation Commission","doi-asserted-by":"publisher","award":["JCYJ20170307095606266"],"award-info":[{"award-number":["JCYJ20170307095606266"]}],"id":[{"id":"10.13039\/501100010877","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Guangdong Province","doi-asserted-by":"publisher","award":["2017B020209001"],"award-info":[{"award-number":["2017B020209001"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Young Innovative Talents Project of General Colleges and Universities in Guangdong Province","doi-asserted-by":"publisher","award":["2018KQNCX010"],"award-info":[{"award-number":["2018KQNCX010"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Guangdong Province","doi-asserted-by":"publisher","award":["2019B020229001"],"award-info":[{"award-number":["2019B020229001"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"First Affiliated Hospital, Jinan University","doi-asserted-by":"publisher","award":["809001"],"award-info":[{"award-number":["809001"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,8,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ankylosing spondylitis (AS) is a chronic immune-mediated disease. Various immune cells play an essential role in the AS pathogenesis. However, the specific pathogenesis of AS has not been well understood. Proteomic profiles of peripheral blood mononuclear cells (PBMCs) were applied to reveal the specific pathogenesis of AS. Quantitative proteomic analyses were performed using liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS)-based methods to investigate the protein profiling of PBMCs from new-onset AS patients (n = 9) and healthy controls (n = 9). We identified 782 differentially expressed proteins (DEPs) and 527 differentially phosphorylated proteins (DPPs) between AS patients and healthy controls. The subcellular location of DEPs and DPPs showed that most of the DEPs were from the cytoplasm (n = 296, 38%), were extracellular (n = 141, 18%), and from the nucleus (n = 114, 15%); most of the DPPs were from the cytoplasm (n = 37, 34%), nucleus (n = 35, 32%), and plasma membrane (n = 10, 9%). We further identified 89 proteins with both expression and phosphorylation differences. The functional annotation of the 89 differentially expressed and phosphorylated proteins enriched in the antigen processing and presentation pathway. Four DEPs with six phosphorylated positions were found in the antigen processing and presentation pathway. The differentially expressed and phosphorylated proteins may be helpful to uncover the pathogenesis of AS. The six AS-specific proteins may serve as candidate markers for AS diagnosis and new treatment targets.<\/jats:p>","DOI":"10.1021\/acsomega.0c01776","type":"journal-article","created":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T20:10:56Z","timestamp":1596485456000},"page":"20153-20161","source":"Crossref","is-referenced-by-count":10,"title":["Quantitative Proteomic Analyses To Reveal the Key Features of Proteins\nin New Onset Ankylosing Spondylitis Patients"],"prefix":"10.1021","volume":"5","author":[{"given":"Yong-Ping","family":"Lu","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Jinan University , , ,","place":["Guangzhou, China, CN 510632"]},{"name":"University of Heidelberg , , , ,","place":["Mannheim, Baden-Wu\u0308rttemberg, Germany, DE 68135"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Heidelberg , , , ,","place":["Mannheim, Baden-Wu\u0308rttemberg, Germany, DE 68135"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengping","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shenzhen People\u2019s Hospital , , ,","place":["Shenzhen, China, CN 518020"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Yun","sequence":"additional","affiliation":[{"name":"Charite\u0301\u2212Universita\u0308tsmedizin Berlin , , Campus Mitte , ,","place":["Berlin, Germany, DE 10117"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengxin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shenzhen People\u2019s Hospital , , ,","place":["Shenzhen, China, CN 518020"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanxia","family":"Cai","sequence":"additional","affiliation":[{"name":"Shenzhen People\u2019s Hospital , , ,","place":["Shenzhen, China, CN 518020"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongzhou","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenzhen People\u2019s Hospital , , ,","place":["Shenzhen, China, CN 518020"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Hong","sequence":"additional","affiliation":[{"name":"Shenzhen People\u2019s Hospital , , ,","place":["Shenzhen, China, CN 518020"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Li","sequence":"additional","affiliation":[{"name":"Dongguan Hospital of Traditional Chinese Medicine , , ,","place":["Dongguan, China, CN 523000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Hu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Jinan University , , ,","place":["Guangzhou, China, CN 510632"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donge","family":"Tang","sequence":"additional","affiliation":[{"name":"Shenzhen People\u2019s Hospital , , ,","place":["Shenzhen, China, CN 518020"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang-Hong","family":"Yin","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Jinan University , , ,","place":["Guangzhou, China, CN 510632"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6438-1311","authenticated-orcid":true,"given":"Yong","family":"Dai","sequence":"additional","affiliation":[{"name":"Shenzhen People\u2019s Hospital , , ,","place":["Shenzhen, China, CN 518020"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,8,3]]},"reference":[{"key":"2026090207114945500_cit1","doi-asserted-by":"publisher","first-page":"1373","DOI":"10.1136\/annrheumdis-2014-205253","article-title":"The epidemiology\nof extra-articular manifestations in ankylosing spondylitis: a population-based\nmatched cohort study","volume":"74","author":"Stolwijk","year":"2015","journal-title":"Ann. Rheum. Dis."},{"key":"2026090207114945500_cit2","first-page":"314","article-title":"HL-A27 and ankylosing spondylitis in B.C. Indians","volume":"2","author":"Gofton","year":"1975","journal-title":"J. Rheumatol."},{"key":"2026090207114945500_cit3","doi-asserted-by":"publisher","first-page":"536","DOI":"10.1590\/s0482-50042012000400006","article-title":"The association\nbetween serum levels of potential biomarkers with the presence of\nfactors related to the clinical activity and poor prognosis in spondyloarthritis","volume":"52","author":"Londono","year":"2012","journal-title":"Rev. Bras. Reumatol."},{"key":"2026090207114945500_cit4","doi-asserted-by":"publisher","first-page":"171573","DOI":"10.1155\/2013\/171573","article-title":"Allicin attenuates\ninflammation and suppresses HLA-B27 protein expression in ankylosing\nspondylitis mice","volume":"2013","author":"Gu","year":"2013","journal-title":"BioMed Res. Int."},{"key":"2026090207114945500_cit5","doi-asserted-by":"publisher","first-page":"601","DOI":"10.1016\/j.berh.2006.03.004","article-title":"Major histocompatibility\ngenes and ankylosing spondylitis","volume":"20","author":"Reveille","year":"2006","journal-title":"Best Pract.\nRes., Clin. Rheumatol."},{"key":"2026090207114945500_cit6","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1093\/intimm\/dxh270","article-title":"The involvement\nof NK cells in ankylosing spondylitis","volume":"17","author":"Azuz-Lieberman","year":"2005","journal-title":"Int.\nImmunol."},{"key":"2026090207114945500_cit7","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1016\/s0161-5890(02)00030-5","article-title":"A structural perspective\non MHC class I recognition by killer cell immunoglobulin-like receptors","volume":"38","author":"Boyington","year":"2002","journal-title":"Mol. Immunol."},{"key":"2026090207114945500_cit8","doi-asserted-by":"publisher","first-page":"R569","DOI":"10.1186\/ar1698","article-title":"Synovial histopathology\nof psoriatic arthritis, both oligo- and polyarticular, resembles spondyloarthropathy\nmore than it does rheumatoid arthritis","volume":"7","author":"Kruithof","year":"2005","journal-title":"Arthritis\nRes. Ther."},{"key":"2026090207114945500_cit9","doi-asserted-by":"publisher","first-page":"820","DOI":"10.1016\/j.autrev.2016.06.001","article-title":"Detection of novel diagnostic antibodies in ankylosing\nspondylitis: An overview","volume":"15","author":"Quaden","year":"2016","journal-title":"Autoimmun. Rev."},{"key":"2026090207114945500_cit10","doi-asserted-by":"publisher","first-page":"4309","DOI":"10.1007\/s00018-015-2005-0","article-title":"Human dendritic\ncell subsets and function in health and disease","volume":"72","author":"O\u2019Keeffe","year":"2015","journal-title":"Cell. Mol. Life Sci."},{"key":"2026090207114945500_cit11","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1093\/rheumatology\/kev245","article-title":"Ankylosing spondylitis patients display altered dendritic\ncell and T cell populations that implicate pathogenic roles for the\nIL-23 cytokine axis and intestinal inflammation","volume":"55","author":"Wright","year":"2016","journal-title":"Rheumatology"},{"key":"2026090207114945500_cit12","doi-asserted-by":"publisher","first-page":"5864","DOI":"10.1021\/ac800337v","article-title":"Quantification of\nprotein phosphorylation by liquid chromatography-mass spectrometry","volume":"80","author":"Previs","year":"2008","journal-title":"Anal. Chem."},{"key":"2026090207114945500_cit13","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1002\/art.1780270401","article-title":"Evaluation of diagnostic criteria for ankylosing spondylitis. A proposal\nfor modification of the New York criteria","volume":"27","author":"Linden","year":"1984","journal-title":"Arthritis\nRheum."},{"key":"2026090207114945500_cit14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-1-4939-6747-6_1","article-title":"A Robust Protocol\nfor Protein Extraction and Digestion","volume":"1550","author":"Atallah","year":"2017","journal-title":"Methods\nMol. Biol."},{"key":"2026090207114945500_cit15","first-page":"209","article-title":"Study on the association of transporter\nassociated with antigen processing gene with ankylosing spondylitis\nand HLA-B27 in Han nationality","volume":"18","author":"Wang","year":"2001","journal-title":"Zhonghua Yixue\nYichuanxue Zazhi"},{"key":"2026090207114945500_cit16","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1007\/s00011-017-1047-1","article-title":"Genetic association between TAP1\nand TAP2 polymorphisms and ankylosing spondylitis: a systematic review\nand meta-analysis","volume":"66","author":"Qian","year":"2017","journal-title":"Inflammation Res."},{"key":"2026090207114945500_cit17","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.mehy.2012.10.003","article-title":"Association\nof ankylosing spondylitis with HLA-B27 and ERAP1: pathogenic role\nof antigenic peptide","volume":"80","author":"Chen","year":"2013","journal-title":"Med. Hypotheses"},{"key":"2026090207114945500_cit18","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1136\/annrheumdis-2016-209512","article-title":"Position 97 of HLA-B, a residue implicated in pathogenesis\nof ankylosing spondylitis, plays a key role in cell surface free heavy\nchain expression","volume":"76","author":"Chen","year":"2017","journal-title":"Ann. Rheum. Dis."},{"key":"2026090207114945500_cit19","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1007\/978-1-4419-0298-6_15","article-title":"Biochemical\nfeatures of HLA-B27 and antigen processing","volume":"649","author":"Powis","year":"2009","journal-title":"Adv. Exp. Med. Biol."},{"key":"2026090207114945500_cit20","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1136\/ard.53.1.58","article-title":"Analysis of the MHC class II encoded components of\nthe HLA class I antigen processing pathway in ankylosing spondylitis","volume":"53","author":"Burney","year":"1994","journal-title":"Ann. Rheum. Dis."},{"key":"2026090207114945500_cit21","first-page":"79","article-title":"KIR3DL1 interaction with HLA-B27 is altered by ankylosing\nspondylitis associated ERAP1 and enhanced by MHC class I cross-linking","volume":"20","author":"Abdullah","year":"2015","journal-title":"Discov. Med."},{"key":"2026090207114945500_cit22","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1007\/s00296-005-0614-x","article-title":"HLA-B27 polymorphism\nin Turkish patients with ankylosing spondylitis","volume":"26","author":"Birinci","year":"2006","journal-title":"Rheumatol. Int."},{"key":"2026090207114945500_cit23","doi-asserted-by":"publisher","first-page":"1943","DOI":"10.3892\/mmr.2017.6248","article-title":"Role of HLA-B27 in\nthe pathogenesis of ankylosing spondylitis","volume":"15","author":"Chen","year":"2017","journal-title":"Mol. Med. Rep."},{"key":"2026090207114945500_cit24","doi-asserted-by":"publisher","first-page":"266","DOI":"10.4081\/reumatismo.2002.266","article-title":"The role of HLA-B27 molecules in\nthe pathogenesis of ankylosing spondylitis","volume":"54","author":"Cauli","year":"2002","journal-title":"Reumatismo"},{"key":"2026090207114945500_cit25","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1002\/art.20948","article-title":"HLA-B27-restricted\nCD8+ T cell response to cartilage-derived self peptides in ankylosing\nspondylitis","volume":"52","author":"Atagunduz","year":"2005","journal-title":"Arthritis Rheum."},{"key":"2026090207114945500_cit26","doi-asserted-by":"publisher","DOI":"10.1136\/rmdopen-2017-000597","article-title":"Expression\nanalysis of HLA-E and NKG2A and NKG2C receptors points at a role for\nnatural killer function in ankylosing spondylitis","volume":"4","author":"Cauli","year":"2018","journal-title":"RMD Open"},{"key":"2026090207114945500_cit27","doi-asserted-by":"publisher","first-page":"R171","DOI":"10.1186\/ar2860","article-title":"HLA-E gene\npolymorphism associates with ankylosing spondylitis in Sardinia","volume":"11","author":"Paladini","year":"2009","journal-title":"Arthritis Res. Ther."},{"key":"2026090207114945500_cit28","doi-asserted-by":"publisher","DOI":"10.1136\/rmdopen-2018-000677","article-title":"Non-classical\nhuman leucocyte antigens in ankylosing spondylitis: possible association\nwith HLA-E and HLA-F","volume":"4","author":"Santos","year":"2018","journal-title":"RMD Open"},{"key":"2026090207114945500_cit29","doi-asserted-by":"publisher","first-page":"5496","DOI":"10.3390\/ijms20215496","article-title":"Dimorphism of HLA-E and its Disease\nAssociation","volume":"20","author":"Kanevskiy","year":"2019","journal-title":"Int. J. Mol. Sci."},{"key":"2026090207114945500_cit30","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/s0049-0172(05)80028-9","article-title":"Heat shock (stress) proteins and autoimmunity in rheumatic\ndiseases","volume":"22","author":"Schultz","year":"1993","journal-title":"Semin. Arthritis Rheum."},{"key":"2026090207114945500_cit31","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/j.autrev.2008.12.004","article-title":"Heat-shock proteins can promote as\nwell as regulate autoimmunity","volume":"8","author":"Rajaiah","year":"2009","journal-title":"Autoimmun. Rev."},{"key":"2026090207114945500_cit32","doi-asserted-by":"publisher","first-page":"621417","DOI":"10.1155\/2013\/621417","article-title":"Heat-shock proteins in autoimmunity","volume":"2013","author":"Moudgil","year":"2013","journal-title":"Autoimmune Dis."},{"key":"2026090207114945500_cit33","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.semarthrit.2009.10.002","article-title":"The involvement\nof heat-shock proteins in the pathogenesis of autoimmune arthritis:\na critical appraisal","volume":"40","author":"Huang","year":"2010","journal-title":"Semin. Arthritis Rheum."},{"key":"2026090207114945500_cit34","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1186\/1471-2474-15-253","article-title":"Inhibition\nof adjuvant-induced arthritis by nasal administration of novel synthetic\npeptides from heat shock protein 65","volume":"15","author":"Shi","year":"2014","journal-title":"BMC Musculoskeletal\nDisord."},{"key":"2026090207114945500_cit35","doi-asserted-by":"publisher","first-page":"55","DOI":"10.2147\/oarrr.s162512","article-title":"Serum heat-shock protein-65\nantibody levels are elevated but not associated with disease activity\nin patients with rheumatoid arthritis and ankylosing spondylitis","volume":"10","author":"Ulusoy","year":"2018","journal-title":"Open Access Rheumatol.: Res. Rev."},{"key":"2026090207114945500_cit36","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1158\/2326-6066.cir-18-0124","article-title":"Calnexin Impairs the Antitumor Immunity\nof CD4(+) and CD8(+) T Cells","volume":"7","author":"Chen","year":"2019","journal-title":"Cancer Immunol.\nRes."},{"key":"2026090207114945500_cit37","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1016\/j.ymeth.2004.10.005","article-title":"In vitro\nand in vivo assays to assess the functions of calnexin and calreticulin\nin ER protein folding and quality control","volume":"35","author":"Paquet","year":"2005","journal-title":"Methods"},{"key":"2026090207114945500_cit38","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1002\/kjm2.12184","article-title":"Aberrant distribution\nand function of plasmacytoid dendritic cells in patients with ankylosing\nspondylitis are associated with unfolded protein response","volume":"36","author":"Liu","year":"2020","journal-title":"Kaohsiung J. Med. Sci."},{"key":"2026090207114945500_cit39","doi-asserted-by":"publisher","first-page":"2438","DOI":"10.4049\/jimmunol.175.4.2438","article-title":"HLA-B27 misfolding in transgenic\nrats is associated with activation of the unfolded protein response","volume":"175","author":"Turner","year":"2005","journal-title":"J. Immunol."},{"key":"2026090207114945500_cit40","doi-asserted-by":"publisher","first-page":"3807","DOI":"10.1002\/art.30593","article-title":"Ankylosing spondylitis macrophage production of higher\nlevels of interleukin-23 in response to lipopolysaccharide without\ninduction of a significant unfolded protein response","volume":"63","author":"Zeng","year":"2011","journal-title":"Arthritis Rheum."},{"key":"2026090207114945500_cit41","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1080\/14397595.2016.1259716","article-title":"Ankylosing\nspondylitis M-CSF-derived macrophages are undergoing unfolded protein\nresponse (UPR) and express higher levels of interleukin-23","volume":"27","author":"Rezaiemanesh","year":"2017","journal-title":"Mod. Rheumatol."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.0c01776","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/5\/32\/20153\/67701073\/acsomega.0c01776.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/5\/32\/20153\/67701073\/acsomega.0c01776.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:12:07Z","timestamp":1788347527000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/5\/32\/20153\/500734\/Quantitative-Proteomic-Analyses-To-Reveal-the-Key"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,3]]},"references-count":41,"journal-issue":{"issue":"32","published-online":{"date-parts":[[2020,8,3]]},"published-print":{"date-parts":[[2020,8,18]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.0c01776","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,3]]}}}