{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:12:32Z","timestamp":1789992752415,"version":"4.0.1"},"reference-count":35,"publisher":"American Chemical Society (ACS)","issue":"50","license":[{"start":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T00:00:00Z","timestamp":1607904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_ccbyncnd_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100011531","name":"University of Alabama","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011531","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,12,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The \u03b2-galactosidase enzyme is a common reporter enzyme that has been used extensively in microbiological and synthetic biology research. Here, we demonstrate that caffeine and theophylline, common natural methylxanthine products found in many foods and pharmaceuticals, negatively impact both the expression and activity of \u03b2-galactosidase in Escherichia coli. The \u03b2-galactosidase activity in E. coli grown with increasing concentrations of caffeine and theophylline was reduced over sixfold in a dose-dependent manner. We also observed decreasing lacZ mRNA transcript levels with increasing methylxanthine concentrations in the growth media. Similarly, caffeine and theophylline inhibit the activity of the purified \u03b2-galactosidase enzyme, with an approximately 1.7-fold increase in KM toward o-nitrophenyl-\u03b2-galactoside and a concomitant decrease in vmax. The authors recommend the use of alternative reporter systems when such methylxanthines are expected to be present.<\/jats:p>","DOI":"10.1021\/acsomega.0c03909","type":"journal-article","created":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T00:51:28Z","timestamp":1607907088000},"page":"32250-32255","source":"Crossref","is-referenced-by-count":17,"title":["Caffeine and Theophylline Inhibit \u03b2-Galactosidase\nActivity and Reduce Expression in\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1021","volume":"5","author":[{"given":"Jesse","family":"Horne","sequence":"first","affiliation":[{"name":"The University of Alabama , , , ,","place":["Tuscaloosa, Alabama, United States, 35487"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Beddingfield","sequence":"additional","affiliation":[{"name":"The University of Alabama , , , ,","place":["Tuscaloosa, Alabama, United States, 35487"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madison","family":"Knapp","sequence":"additional","affiliation":[{"name":"The University of Alabama , , , ,","place":["Tuscaloosa, Alabama, United States, 35487"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Mitchell","sequence":"additional","affiliation":[{"name":"The University of Alabama , , , ,","place":["Tuscaloosa, Alabama, United States, 35487"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Logan","family":"Crawford","sequence":"additional","affiliation":[{"name":"The University of Alabama , , , ,","place":["Tuscaloosa, Alabama, United States, 35487"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelby Brooks","family":"Mills","sequence":"additional","affiliation":[{"name":"The University of Alabama , , , ,","place":["Tuscaloosa, Alabama, United States, 35487"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Wrist","sequence":"additional","affiliation":[{"name":"The University of Alabama , , , ,","place":["Tuscaloosa, Alabama, United States, 35487"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"The University of Alabama , , , ,","place":["Tuscaloosa, Alabama, United States, 35487"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1358-6605","authenticated-orcid":true,"given":"Ryan M.","family":"Summers","sequence":"additional","affiliation":[{"name":"The University of Alabama , , , ,","place":["Tuscaloosa, Alabama, United States, 35487"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,12,14]]},"reference":[{"key":"2026092106131929300_cit1","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1021\/es020125z","article-title":"Caffeine, an anthropogenic\nmarker for wastewater contamination of surface waters","volume":"37","author":"Buerge","year":"2003","journal-title":"Environ. 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