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However, prokaryotic organisms lack subcellular organelles, and protein localization based on subcellular locations like cytoplasm, inner membrane, periplasm, and outer membrane can be accounted for functional characterization. We have identified 131 acetylated, 1182 citrullinated, 72 glutarylated, 5 palmitoylated, and 139 phosphorylated proteins from Triton X-114 fractionated proteins of Leptospira, the pathogen of re-emerging zoonotic disease leptospirosis. In total, 74.7% of proteins were found exclusively in different Triton X-114 fractions. Additionally, 21.9% of proteins in multiple fractions had one or more PTM specific to different Triton X-114 fractions. Altogether, 96.6% of proteins showed exclusiveness to different Triton X-114 fractions either due to the presence of the entire protein or with a specific PTM type or position. Further, the PTM distribution within Triton X-114 fractions showed higher acetylation in aqueous, glutarylation in detergent, phosphorylation in pellet, and citrullination in wash fractions representing cytoplasmic, outer membrane, inner membrane, and extracellular locations, respectively. Identification of PTMs in proteins with respect to the subcellular localization will help to characterize candidate proteins before developing novel drugs and vaccines rationally to combat leptospirosis.<\/jats:p>","DOI":"10.1021\/acsomega.2c01245","type":"journal-article","created":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T12:19:21Z","timestamp":1653481161000},"page":"18569-18576","source":"Crossref","is-referenced-by-count":7,"title":["Unique Posttranslational Modification Sites of Acetylation, Citrullination,\nGlutarylation, and Phosphorylation Are Found to Be Specific to the\nProteins Partitioned in the Triton X\u2011114 Fractions of\n                    <i>Leptospira<\/i>"],"prefix":"10.1021","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8781-8870","authenticated-orcid":true,"given":"Homen","family":"Phukan","sequence":"first","affiliation":[{"name":"ICMR-Regional Medical Research Centre , , ,","place":["Port Blair, Andaman and Nicobar\nIslands, India, 744103"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhijit","family":"Sarma","sequence":"additional","affiliation":[{"name":"ICMR-Regional Medical Research Centre , , ,","place":["Port Blair, Andaman and Nicobar\nIslands, India, 744103"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9556-3150","authenticated-orcid":true,"given":"Devasahayam Arokia\nBalaya","family":"Rex","sequence":"additional","affiliation":[{"name":"Yenepoya (Deemed to be University) , , , ,","place":["Mangaluru, Karnataka, India, 575018"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhila Balakrishna","family":"Rai","sequence":"additional","affiliation":[{"name":"Yenepoya (Deemed to be University) , , , ,","place":["Mangaluru, Karnataka, India, 575018"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6206-2384","authenticated-orcid":true,"given":"Thottethodi Subrahmanya\nKeshava","family":"Prasad","sequence":"additional","affiliation":[{"name":"Yenepoya (Deemed to be University) , , , ,","place":["Mangaluru, Karnataka, India, 575018"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5119-0081","authenticated-orcid":true,"given":"Madathiparambil Gopalakrishnan","family":"Madanan","sequence":"additional","affiliation":[{"name":"ICMR-Regional Medical Research Centre , , ,","place":["Port Blair, Andaman and Nicobar\nIslands, India, 744103"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"2026081710182672500_cit1","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1111\/j.1469-0691.2011.03474.x","article-title":"Emergence, Control and Re-Emerging\nLeptospirosis: Dynamics of Infection in the Changing World","volume":"17","author":"Hartskeerl","year":"2011","journal-title":"Clin. 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