{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T13:31:00Z","timestamp":1786973460877,"version":"build-2736575974"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81602773"],"award-info":[{"award-number":["81602773"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Androgenetic alopecia (AGA) is a major problem that can happen to people of all ages, leading to psychological problems, such as anxiety and depression. Topical Shen Bai hair growing decoction (TSBHGD) is based on the pathogenesis of AGA, combined with Traditional Chinese Medicine theory, improved by the Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital according to its clinical treatment experience. This study was designed to demonstrate the therapeutic efficacy of TSBHGD against AGA, analyze the chemical components of TSBHGD as well as the skin-retained and blood-retained components in mice after topical administration of TSBHGD, and clarify the mechanism of its therapeutic efficacy. It was demonstrated that TSBHGD could suppress TNF-\u03b1 and IL-6 levels and improve pathological phenomena such as hair loss, reduced follicle density, and dermal thickness caused by testosterone solution. Totally 35 components were identified in TSBHGD extracts, 12 skin-retained components were identified in drug-containing skin, and 7 blood-retained components were identified in drug-containing plasma, according to ultrahigh performance liquid chromatography quadrupole time-of-flight mass spectrometry. Transcriptomic sequencing revealed that some of the genes in AGA mice had altered expression patterns, which could be reversed by TSBHGD. Through network pharmacology analysis, it was found that TSBHGD mainly regulated eight signaling pathways, among which the apoptosis signaling pathway ranked first with a significance of 0.00149. Finally, both Bcl-2 and Caspase family proteins in the apoptosis signaling pathway were examined by Western blot. It was confirmed that TSBHGD could inhibit the apoptosis level in AGA mice\u2019s skin tissue to exert an anti-AGA effect. This will facilitate the development of new-generation herbal compound formulas with precise efficacy and provide novel ideas for AGA therapy.<\/jats:p>","DOI":"10.1021\/acsomega.3c09648","type":"journal-article","created":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:39:50Z","timestamp":1708907990000},"page":"10834-10851","source":"Crossref","is-referenced-by-count":6,"title":["Study on the Efficacy and Potential Mechanism of Topical\nShen Bai Hair Growing Decoction against Androgenetic Alopecia Based\non Ultrahigh Performance Liquid Chromatography Quadrupole Time-of-Flight\nMass Spectrometry and RNA-seq"],"prefix":"10.1021","volume":"9","author":[{"given":"Mingxi","family":"Li","sequence":"first","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine , , ,","place":["Tianjin, China, 300193"]},{"name":"Tianjin University of Traditional Chinese Medicine , , ,","place":["Tianjin, China, 300193"]},{"name":"Tianjin University of Traditional Chinese Medicine , ,","place":["Tianjin, China, 300193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital , ,","place":["Tianjin, China, 300120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1757-513X","authenticated-orcid":true,"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine , , ,","place":["Tianjin, China, 300193"]},{"name":"Tianjin University of Traditional Chinese Medicine , , ,","place":["Tianjin, China, 300193"]},{"name":"Tianjin University of Traditional Chinese Medicine , ,","place":["Tianjin, China, 300193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beibei","family":"Xiang","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine , ,","place":["Tianjin, China, 300193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoyi","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine , ,","place":["Tianjin, China, 300193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine , ,","place":["Tianjin, China, 300193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanming","family":"Liu","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine , ,","place":["Tianjin, China, 300193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoxi","family":"Guo","sequence":"additional","affiliation":[{"name":"Tianjin Shangmei Cosmetics Co., Ltd , ,","place":["Tianjin, China, 301617"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2024,2,26]]},"reference":[{"issue":"5","key":"2026081709164860500_cit1","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1007\/s00403-018-1826-8","article-title":"Androgen modulation of Wnt\/\u03b2-catenin signaling\nin androgenetic alopecia","volume":"310","author":"Premanand","year":"2018","journal-title":"Arch. 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Dermatol.\nSci."},{"issue":"8","key":"2026081709164860500_cit23","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/s11934-018-0814-z","article-title":"The Impact of 5\u03b1-Reductase\nInhibitor Use for Male Pattern Hair\nLoss on Men\u2019s Health","volume":"19","author":"Said","year":"2018","journal-title":"Curr. Neurol. Rep."},{"issue":"3","key":"2026081709164860500_cit24a","doi-asserted-by":"publisher","DOI":"10.1111\/dth.13379","article-title":"5-Alpha\nreductase inhibitors in androgenetic alopecia: Shifting paradigms,\ncurrent concepts, comparative efficacy, and safety","volume":"33","author":"Dhurat","year":"2020","journal-title":"Dermatol. Ther."},{"issue":"15","key":"2026081709164860500_cit24b","doi-asserted-by":"publisher","first-page":"5342","DOI":"10.3390\/ijms21155342","article-title":"Hormonal Effects\non Hair Follicles","volume":"21","author":"Grymowicz","year":"2020","journal-title":"Int. J. Mol. 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Dermatol."},{"issue":"1","key":"2026081709164860500_cit27","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1038\/cdd.2017.186","article-title":"BCL-2 family proteins: changing partners\nin the dance towards death","volume":"25","author":"Kale","year":"2018","journal-title":"Cell Death Differ."},{"key":"2026081709164860500_cit28","doi-asserted-by":"publisher","first-page":"112907","DOI":"10.1016\/j.jep.2020.112907","article-title":"A comprehensive review\nof biochemical factors in herbs and their\nconstituent compounds in experimental studies on alopecia","volume":"258","author":"Cho","year":"2020","journal-title":"J. Ethnopharmacol."},{"issue":"01\/02","key":"2026081709164860500_cit29","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1055\/s-0035-1557897","article-title":"Topical\nTreatment of Hair Loss with Formononetin by Modulating Apoptosis","volume":"82","author":"Kim","year":"2016","journal-title":"Planta Med."},{"key":"2026081709164860500_cit30","doi-asserted-by":"publisher","first-page":"115320","DOI":"10.1016\/j.jep.2022.115320","article-title":"Exploring the effects\nof Chinese herbal ingredients on the signaling pathway of alopecia\nand the screening of effective Chinese herbal compounds","volume":"294","author":"Dou","year":"2022","journal-title":"J. Ethnopharmacol."},{"key":"2026081709164860500_cit31","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.ddtec.2015.05.001","article-title":"Network-based\npharmacology through systems biology","volume":"15","author":"Westerhoff","year":"2015","journal-title":"Drug Discovery\nToday: Technol."},{"issue":"3","key":"2026081709164860500_cit32a","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.jdermsci.2019.02.004","article-title":"Evaluating\nhair growth promoting effects of candidate substance: A review of\nresearch methods","volume":"93","author":"Ohn","year":"2019","journal-title":"J. Dermatol. 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