{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T17:54:45Z","timestamp":1793382885700,"version":"4.5.0"},"reference-count":29,"publisher":"American Chemical Society (ACS)","issue":"28","license":[{"start":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T00:00:00Z","timestamp":1719532800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100012554","name":"Hubei Provincial Department of Education","doi-asserted-by":"publisher","award":["D20222702"],"award-info":[{"award-number":["D20222702"]}],"id":[{"id":"10.13039\/100012554","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018902","name":"Xiaogan Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["XGKJ2021010013"],"award-info":[{"award-number":["XGKJ2021010013"]}],"id":[{"id":"10.13039\/100018902","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52275427"],"award-info":[{"award-number":["52275427"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2022CFB533"],"award-info":[{"award-number":["2022CFB533"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003819","name":"Natural Science Foundation of Hubei Province","doi-asserted-by":"publisher","award":["2023AFB976"],"award-info":[{"award-number":["2023AFB976"]}],"id":[{"id":"10.13039\/501100003819","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018621","name":"Program for Changjiang Scholars and Innovative Research Team in University","doi-asserted-by":"publisher","award":["IRT_17R41"],"award-info":[{"award-number":["IRT_17R41"]}],"id":[{"id":"10.13039\/501100018621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,7,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Biomachining is an eco-friendly metal processing method with broad application potential. Nevertheless, the bacterial culture methods that are currently involved in biomachining require the intensive use of chemical reagents, especially FeSO4, specialized equipment, and professional-level skills in the field of biology. Herein, the differences between two cultures with and without sterilization were evaluated. Acidithiobacillus ferrooxidans was cultured with iron instead of FeSO4 in the culture medium. The chemical and biochemical parameters of the culture were analyzed by studying the area of exposed iron and continuously regulating the pH. Eliminating the sterilization and sterile inoculation of the medium is feasible for culturing A. ferrooxidans. The key to achieving a high bacterial density in culture with iron was to maintain the solution pH. The possibility of mass culturing A. ferrooxidans with steel cuttings was evaluated in a custom bioreactor, and the bacterial concentration reached 9 \u00d7 107 cells\/mL.<\/jats:p>","DOI":"10.1021\/acsomega.4c04257","type":"journal-article","created":{"date-parts":[[2024,6,28]],"date-time":"2024-06-28T16:54:05Z","timestamp":1719593645000},"page":"30998-31005","source":"Crossref","is-referenced-by-count":9,"title":["Simplification\nof the\n                    <i>Acidithiobacillus\nferrooxidans<\/i>\n                    Culture Process for Expanding the Field\nof Biomachining"],"prefix":"10.1021","volume":"9","author":[{"given":"Fei","family":"Ma","sequence":"first","affiliation":[{"name":"Hubei Engineering University , , , ,","place":["Xiaogan, Hubei Province, P. R. China, 432000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5724-5493","authenticated-orcid":true,"given":"Meie","family":"Zheng","sequence":"additional","affiliation":[{"name":"Hubei Engineering University , , , ,","place":["Xiaogan, Hubei Province, P. R. China, 432000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Huang","sequence":"additional","affiliation":[{"name":"Huaqiao University , , , ,","place":["Xiamen, Fujian Province, P.\nR. China, 361021"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang","family":"Xu","sequence":"additional","affiliation":[{"name":"Hubei Engineering University , , , ,","place":["Xiaogan, Hubei Province, P. R. China, 432000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumei","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Engineering University , , , ,","place":["Xiaogan, Hubei Province, P. R. China, 432000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Xiong","sequence":"additional","affiliation":[{"name":"Hubei Engineering University , , , ,","place":["Xiaogan, Hubei Province, P. R. China, 432000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Hubei Engineering University , , , ,","place":["Xiaogan, Hubei Province, P. R. China, 432000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2024,6,28]]},"reference":[{"issue":"2","key":"2026081708333717600_cit1","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1007\/BF02882720","article-title":"Possibility of Biological Micromachining Used for Metal Removal","volume":"41","author":"Zhang","year":"1998","journal-title":"Sci. China, Ser. 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Precis. Eng.\nManuf. -Green Technol."},{"key":"2026081708333717600_cit5","doi-asserted-by":"publisher","first-page":"376","DOI":"10.16356\/j.1005-1120.2019.03.002","volume-title":"Parameter Optimization\nfor Improvement in Biomachining Performance","author":"Hui","year":"2019"},{"issue":"4","key":"2026081708333717600_cit6","doi-asserted-by":"publisher","first-page":"105283","DOI":"10.1016\/j.jece.2021.105283","article-title":"Immobilization\nof Acidithiobacillus Ferrooxidans in Bacterial Cellulose for a More\nSustainable Bioleaching Process","volume":"9","author":"Santaolalla","year":"2021","journal-title":"J. Environ.\nChem. Eng."},{"issue":"5","key":"2026081708333717600_cit7","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1002\/bab.1464","article-title":"Immobilization\nof Acidithiobacillus Ferrooxidans on Cotton Gauze\nfor Biological Oxidation of Ferrous Ions in a Batch Bioreactor","volume":"64","author":"Zhu","year":"2017","journal-title":"Biotechnol. Appl. 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Chem."},{"issue":"13","key":"2026081708333717600_cit22","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2300085120","article-title":"Generating Dual-Active\nSpecies by Triple-Atom Sites through Peroxymonosulfate\nActivation for Treating Micropollutants in Complex Water","volume":"120","author":"Zhou","year":"2023","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2026081708333717600_cit23","doi-asserted-by":"publisher","first-page":"136387","DOI":"10.1016\/j.jclepro.2023.136387","article-title":"Preparation and Model\nConstruction of Novel 2D Nanocomposite\nof Zn-P-GCNN and Its Mechanisms of Synergistic Adsorption for Cu\u00ad(II)\nand Methylene Blue","volume":"395","author":"Zheng","year":"2023","journal-title":"J. Cleaner Prod."},{"key":"2026081708333717600_cit24","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.hydromet.2013.03.005","article-title":"Isolation\nand Characterization\nof Acidithiobacillus Ferrooxidans Strain QXS-1 Capable of Unusual\nFerrous Iron and Sulfur Utilization","volume":"136","author":"Zhang","year":"2013","journal-title":"Hydrometallurgy"},{"key":"2026081708333717600_cit25","doi-asserted-by":"publisher","first-page":"102801","DOI":"10.1016\/j.eti.2022.102801","article-title":"Bioleaching\nof Carbide Waste Using Spent Culture of Acidithiobacillus Bacteria:\nEffective Factor Evaluation and Ecological Risk Assessment","volume":"28","author":"Pirsaheb","year":"2022","journal-title":"Environ. Technol. Innovation"},{"key":"2026081708333717600_cit26","doi-asserted-by":"publisher","first-page":"159078","DOI":"10.1016\/j.scitotenv.2022.159078","article-title":"Mineral Weathering of Iron Ore Tailings\nPrimed by Acidithiobacillus Ferrooxidans and Elemental Sulfur under\nContrasting pH Conditions","volume":"856","author":"Yi","year":"2023","journal-title":"Sci. Total Environ."},{"key":"2026081708333717600_cit27","doi-asserted-by":"publisher","first-page":"122549","DOI":"10.1016\/j.jclepro.2020.122549","article-title":"Viability\nof Two Alternatives for Treating Waste Solutions from the Biomachining\nProcess","volume":"270","author":"Santaolalla","year":"2020","journal-title":"J. Cleaner Prod."},{"issue":"1","key":"2026081708333717600_cit28","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/S1003-6326(23)66401-5","article-title":"Thiourea Leaching of Gold in Presence of Jarosite and Role of Oxalate","volume":"34","author":"Li","year":"2024","journal-title":"Trans. Nonferrous Met. Soc. China"},{"key":"2026081708333717600_cit29","doi-asserted-by":"publisher","first-page":"106144","DOI":"10.1016\/j.hydromet.2023.106144","article-title":"Influence of Solution\npH on the Properties of Biogenic\nJarosite Produced from Ferrous Ion Bio-Oxidation in a Bioreactor","volume":"221","author":"Oladipo","year":"2023","journal-title":"Hydrometallurgy"}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.4c04257","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/9\/28\/30998\/19608243\/ao4c04257.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/9\/28\/30998\/19608243\/ao4c04257.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T12:33:44Z","timestamp":1786970024000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/9\/28\/30998\/1762012\/Simplification-of-the-Acidithiobacillus"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,28]]},"references-count":29,"journal-issue":{"issue":"28","published-online":{"date-parts":[[2024,6,28]]},"published-print":{"date-parts":[[2024,7,16]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.4c04257","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,28]]}}}