{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:46:10Z","timestamp":1792784770581,"version":"4.5.0"},"reference-count":43,"publisher":"American Chemical Society (ACS)","issue":"42","license":[{"start":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T00:00:00Z","timestamp":1728518400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001774","name":"University of Sydney","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Department of Education and Training","doi-asserted-by":"publisher","award":["DP210103160"],"award-info":[{"award-number":["DP210103160"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Department of Education and Training","doi-asserted-by":"publisher","award":["LP180100309"],"award-info":[{"award-number":["LP180100309"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chuangqi Shidai Qingdao Technology Co. Ltd"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The concentration of nonesterified fatty acids (NEFAs) in biological media is associated with metabolic and cardiovascular disorders (e.g., diabetes, cancer, and cystic fibrosis) and in food products is indicative of their quality. Therefore, the early identification of NEFAs is crucial for both medical diagnosis and food quality assessment. However, the development of a portable and scalable sensor capable of detecting these compounds at a low cost presents challenges due to their considerable chemical and physical stability. This research endeavors to illustrate the viability of detecting linoleic acid using a chemiresistive bienzymatic sensor constructed with cotton thread. The sensor\u2019s design incorporates the conductive polymer poly\u00ad(3,4-ethylenedioxythiophene):polystyrenesulfonate (PEDOT:PSS) within the thread, alongside the enzymes horseradish peroxidase (HRP) and lipoxygenase (LOX). By implementing this technology, a sensitive detection range spanning from 161 nM to 16.1 \u03bcM is achieved when the PEDOT:PSS\/HRP\/LOX system is integrated into a single thread. The sensor exhibits exceptional selectivity toward linoleic acid, owing to the specific enzymatic reaction between LOX and linoleic acid. This selectivity is upheld even in the presence of other unsaturated fatty acids. This system can be used for future designs with the capability to detect polyunsaturated fatty acids and other intricate biomolecules.<\/jats:p>","DOI":"10.1021\/acsomega.4c07394","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T21:40:47Z","timestamp":1728596447000},"page":"43184-43192","source":"Crossref","is-referenced-by-count":10,"title":["Thread-Based Bienzymatic\nBiosensor for Linoleic Acid\nDetection"],"prefix":"10.1021","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0006-2132","authenticated-orcid":true,"given":"Jacopo","family":"Giaretta","sequence":"first","affiliation":[{"name":"The University of Sydney , , , ,","place":["Sydney, NSW, Australia, 2006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7464-8251","authenticated-orcid":true,"given":"Farshad","family":"Oveissi","sequence":"additional","affiliation":[{"name":"The University of Sydney , , , ,","place":["Sydney, NSW, Australia, 2006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9168-6746","authenticated-orcid":true,"given":"Sina","family":"Naficy","sequence":"additional","affiliation":[{"name":"The University of Sydney , , , ,","place":["Sydney, NSW, Australia, 2006"]},{"name":"The University of Sydney , , , ,","place":["Sydney, NSW, Australia, 2006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2997-5931","authenticated-orcid":true,"given":"Syamak","family":"Farajikhah","sequence":"additional","affiliation":[{"name":"The University of Sydney , , , ,","place":["Sydney, NSW, Australia, 2006"]},{"name":"The University of Sydney , , , ,","place":["Sydney, NSW, Australia, 2006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7805-8101","authenticated-orcid":true,"given":"Fariba","family":"Dehghani","sequence":"additional","affiliation":[{"name":"The University of Sydney , , , ,","place":["Sydney, NSW, Australia, 2006"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"2026081412134340800_cit1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/B978-0-12-809521-8.00002-7","article-title":"Naturally occurring\nfatty acids: Source, chemistry, and uses","volume-title":"Fatty acids","author":"Kenar","year":"2017"},{"issue":"1 Suppl","key":"2026081412134340800_cit2","doi-asserted-by":"publisher","first-page":"18S","DOI":"10.1177\/0148607115595980","article-title":"Functional\nRoles of Fatty Acids and Their Effects on Human Health","volume":"39","author":"Calder","year":"2015","journal-title":"J. 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Chem.\nB"},{"issue":"72","key":"2026081412134340800_cit26","doi-asserted-by":"publisher","first-page":"10002","DOI":"10.1039\/C7CC04894G","article-title":"A highly efficient\n2D exfoliated metal dichalcogenide\nfor the on-farm rapid monitoring of non-esterified fatty acids","volume":"53","author":"Tuteja","year":"2017","journal-title":"Chem. Commun."},{"issue":"22","key":"2026081412134340800_cit27","doi-asserted-by":"publisher","first-page":"9075","DOI":"10.1109\/JSEN.2018.2870071","article-title":"Simultaneous Electrochemical Detection of Glucose and Non-Esterified\nFatty Acids (NEFAs) for Diabetes Management","volume":"18","author":"Sahin","year":"2018","journal-title":"IEEE Sens. 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Acta"},{"issue":"3","key":"2026081412134340800_cit30a","doi-asserted-by":"publisher","first-page":"390","DOI":"10.1016\/j.bbalip.2013.08.005","article-title":"The role of\nlipoxygenases in epidermis","volume":"1841","author":"Krieg","year":"2014","journal-title":"Biochim. Biophys.\nActa"},{"issue":"10","key":"2026081412134340800_cit30b","doi-asserted-by":"publisher","first-page":"5866","DOI":"10.1021\/cr200246d","article-title":"Lipoxygenase and Leukotriene Pathways:\nBiochemistry, Biology, and Roles in Disease","volume":"111","author":"Haeggstro\u0308m","year":"2011","journal-title":"Chem. Rev."},{"issue":"2","key":"2026081412134340800_cit31","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116195","article-title":"Comprehensive profiling of plasma\nfatty acid concentrations in young healthy Canadian adults","volume":"10","author":"Abdelmagid","year":"2015","journal-title":"PLoS One"},{"issue":"9","key":"2026081412134340800_cit32","doi-asserted-by":"publisher","first-page":"3690","DOI":"10.3168\/jds.S0022-0302(06)72409-2","article-title":"Estimating\nFatty Acid Content in Cow Milk Using Mid-Infrared Spectrometry","volume":"89","author":"Soyeurt","year":"2006","journal-title":"J. Dairy Sci."},{"issue":"11","key":"2026081412134340800_cit33","doi-asserted-by":"publisher","DOI":"10.1002\/admt.201800137","article-title":"Printed, Flexible pH Sensor Hydrogels\nfor Wet Environments","volume":"3","author":"Naficy","year":"2018","journal-title":"Adv. Mater. 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Technol."},{"key":"2026081412134340800_cit36","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.mattod.2022.07.015","article-title":"PEDOT:PSS-modified\ncotton conductive thread for mass manufacturing of textile-based electrical\nwearable sensors by computerized embroidery","volume":"59","author":"Alshabouna","year":"2022","journal-title":"Mater. Today"},{"key":"2026081412134340800_cit37a","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/j.snb.2015.10.009","article-title":"Electrical properties of multi-walled\ncarbon nanotubes\/PEDOT:PSS nanocomposites thin films under temperature\nand humidity effects","volume":"224","author":"Benchirouf","year":"2016","journal-title":"Sens. Actuators B Chem."},{"key":"2026081412134340800_cit37b","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/j.snb.2019.05.018","article-title":"Dopant passivation\nby adsorbed water monomers causes high humidity sensitivity in PEDOT:\nPSS thin films at ppm-level humidity","volume":"293","author":"Hossein-Babaei","year":"2019","journal-title":"Sens.\nActuators B Chem."},{"key":"2026081412134340800_cit38","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1016\/j.bios.2015.11.058","article-title":"Lipoxygenase-modified\nRu-bpy\/graphene\noxide: Electrochemical biosensor for on-farm monitoring of non-esterified\nfatty acid","volume":"78","author":"Veerapandian","year":"2016","journal-title":"Biosens. Bioelectron."}],"container-title":["ACS Omega"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsomega.4c07394","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/9\/42\/43184\/4116320\/ao4c07394.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/acsodf\/article-pdf\/9\/42\/43184\/4116320\/ao4c07394.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:30:42Z","timestamp":1786725042000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/acsodf\/article\/9\/42\/43184\/409806\/Thread-Based-Bienzymatic-Biosensor-for-Linoleic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,10]]},"references-count":43,"journal-issue":{"issue":"42","published-online":{"date-parts":[[2024,10,10]]},"published-print":{"date-parts":[[2024,10,22]]}},"URL":"https:\/\/doi.org\/10.1021\/acsomega.4c07394","relation":{},"ISSN":["2470-1343"],"issn-type":[{"value":"2470-1343","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,10]]}}}